Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,901,038:1 +T 5.8% coding (548/1557 nt) yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,901,0381.T 5.8% 729.7 / 41.2 172coding (548/1557 nt)yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base . (81/81);  new base T (4/6);  total (85/87)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

Only 100 of 186 total aligned reads displayed.

ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAGGA  >  NC_000913/1900890‑1901185
                                                                                                                                                     |                                                                                                                                                   
aCGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg                                                                                                                                                      >  1:1001310/1‑149 (MQ=255)
aCGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg                                                                                                                                                      <  2:150220/149‑1 (MQ=255)
 cgcgCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                                                                                                                                    <  3:1217420/149‑1 (MQ=255)
 cgcgCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                                                                                                                                    <  1:280475/149‑1 (MQ=255)
 cgcgCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                                                                                                                                    <  1:1537851/149‑1 (MQ=255)
  gcgcAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgggg                                                                                                                                                   >  2:964730/1‑149 (MQ=255)
   cgcAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCTTGGGGt                                                                                                                                                  <  2:1128088/149‑1 (MQ=255)
    gcAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTg                                                                                                                                                 >  2:374672/1‑149 (MQ=255)
    gcAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTg                                                                                                                                                 >  1:947705/1‑149 (MQ=255)
      aaTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCt                                                                                                                                               >  1:250549/1‑149 (MQ=255)
      aaTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCt                                                                                                                                               <  1:517919/149‑1 (MQ=255)
       aTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGATAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCCCACGGTGGTGGTGCtc                                                                                                                                              <  2:1312889/149‑2 (MQ=255)
        tCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTgg                                                                                                                                             >  2:591214/1‑149 (MQ=255)
        tCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTgg                                                                                                                                             >  2:1568053/1‑149 (MQ=255)
          tGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGtt                                                                                                                                           <  3:1051546/149‑1 (MQ=255)
            gTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTaa                                                                                                                                         >  4:1565624/1‑149 (MQ=255)
            gTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCCCACGGTGGTGGTGCtctgtct                                                                                                                                         <  1:1295324/149‑7 (MQ=37)
                aaCACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGaa                                                                                                                                     <  2:1250111/149‑1 (MQ=255)
                aaCACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGaa                                                                                                                                     >  1:978173/1‑149 (MQ=255)
                 acacTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAAt                                                                                                                                    <  3:1188990/149‑1 (MQ=255)
                  cacTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATc                                                                                                                                   <  4:1439607/149‑1 (MQ=255)
                    cTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATcgc                                                                                                                                 <  1:1219948/149‑1 (MQ=255)
                    cTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATcgc                                                                                                                                 <  2:1265413/149‑1 (MQ=255)
                     ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCCCACGGTGGTGGTGCtct                                                                                                                                             <  1:394842/136‑3 (MQ=37)
                     ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCGCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATcgcg                                                                                                                                <  1:1101291/149‑1 (MQ=255)
                              gatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAAc                                                                                                                                        <  2:971710/132‑1 (MQ=255)
                              gatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTc                                                                                                                              <  4:1469884/142‑1 (MQ=255)
                              gatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTc                                                                                                                              >  3:1469884/1‑142 (MQ=255)
                              gatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGtt                                                                                                                       <  3:845751/149‑1 (MQ=255)
                              gatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGtt                                                                                                                       <  3:1401009/149‑1 (MQ=255)
                               atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                      <  2:1339144/149‑1 (MQ=255)
                               atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                      <  4:83340/149‑1 (MQ=255)
                               atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                      <  2:686360/149‑1 (MQ=255)
                                      aGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                               <  3:781793/149‑1 (MQ=255)
                                       gCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa                                                                                                              <  4:699537/149‑1 (MQ=255)
                                       gCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAACACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa                                                                                                              >  4:1169301/1‑149 (MQ=255)
                                          aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCa                                                                                                           >  4:1229748/1‑149 (MQ=255)
                                                 gCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAc                                                                                                    <  1:1193792/149‑1 (MQ=255)
                                                   aTACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGTGGTTTGGATGCCAGCAACATAACCg                                                                                                  >  1:837406/1‑149 (MQ=255)
                                                     aCAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGcc                                                                                                <  4:178554/149‑1 (MQ=255)
                                                       aGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                                   >  2:907075/1‑128 (MQ=255)
                                                        ggTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGTACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATc                                                                                             <  1:1591062/149‑1 (MQ=255)
                                                         gTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCg                                                                                            >  3:1387253/1‑149 (MQ=255)
                                                               tttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCTCCATCGCaa                                                                                         >  1:1259241/1‑146 (MQ=255)
                                                               tttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCa                                                                                      >  2:1008594/1‑149 (MQ=255)
                                                               gttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCCCCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCACCATCGCAATCa                                                                                      >  4:1360982/2‑149 (MQ=255)
                                                                   ggAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGTGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAc                                                                                  <  2:837406/149‑1 (MQ=255)
                                                                   ggAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAc                                                                                  <  3:533265/149‑1 (MQ=255)
                                                                      aTGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAccgc                                                                               <  4:908446/149‑1 (MQ=255)
                                                                       tgtgGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAccgcc                                                                              >  4:344163/1‑149 (MQ=255)
                                                                         tgGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACcgccgc                                                                            <  1:1008594/149‑1 (MQ=255)
                                                                           gaaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAAc                                                                                                                                        >  1:824966/1‑87 (MQ=255)
                                                                           gaaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAAc                                                                                                                                        <  2:824966/87‑1 (MQ=255)
                                                                            aaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                                                                                                                                    <  1:209106/74‑1 (MQ=255)
                                                                            aaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTggg                                                                                                                                                    >  2:209106/1‑74 (MQ=255)
                                                                             aaccgaaaccTTCTGCCACCAGACTCAGACCAGTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCcatc                                                                        >  3:858813/1‑149 (MQ=255)
                                                                             aaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCcatc                                                                        <  1:374672/149‑1 (MQ=255)
                                                                                cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCatcatc                                                                     >  4:211576/1‑149 (MQ=255)
                                                                                 gaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCAtcatca                                                                    >  4:902671/1‑149 (MQ=255)
                                                                                       ttCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                              >  3:690450/1‑149 (MQ=255)
                                                                                            ccaccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATgg                                                         <  2:17354/149‑1 (MQ=255)
                                                                                            ccaccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATgg                                                         <  4:1049365/149‑1 (MQ=255)
                                                                                              accaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGtt                                                       <  2:345989/149‑1 (MQ=255)
                                                                                               ccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGCTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTa                                                      >  1:78629/1‑149 (MQ=255)
                                                                                                caGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTaa                                                     <  1:663391/149‑1 (MQ=255)
                                                                                                caGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTaa                                                     <  3:368429/149‑1 (MQ=255)
                                                                                                  gACTCAGACCAATCATTATCAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAcc                                                   <  2:700087/149‑1 (MQ=255)
                                                                                                     tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                      >  4:411248/1‑79 (MQ=255)
                                                                                                     tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                      <  3:411248/79‑1 (MQ=255)
                                                                                                     tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATgg                                                         >  4:473320/1‑140 (MQ=255)
                                                                                                     tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATgg                                                         <  3:473320/140‑1 (MQ=255)
                                                                                                         aCCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                            <  1:1378407/149‑1 (MQ=255)
                                                                                                           cAATCATTATCAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAc                                                                                  >  1:1366689/1‑109 (MQ=255)
                                                                                                           cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTATCGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAc                                                                                  <  2:1366689/109‑1 (MQ=255)
                                                                                                             aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCa                                                                                      >  4:1029185/1‑103 (MQ=255)
                                                                                                                aTTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGCTAACCAGCCCTACTGCGGt                                     >  3:1247116/1‑149 (MQ=255)
                                                                                                                 ttAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGc                                                                                                 <  2:723421/88‑1 (MQ=255)
                                                                                                                 ttAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGc                                                                                                 >  1:723421/1‑88 (MQ=255)
                                                                                                                  tAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTaa                                   >  4:43917/1‑149 (MQ=255)
                                                                                                                    aCAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAAtt                                 >  3:628048/1‑149 (MQ=255)
                                                                                                                    aCAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAAtt                                 <  1:623752/149‑1 (MQ=255)
                                                                                                                       ggAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                                   <  2:769851/80‑1 (MQ=255)
                                                                                                                       ggAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                                   >  1:769851/1‑80 (MQ=255)
                                                                                                                       ggAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAcc                              <  1:1370631/149‑1 (MQ=255)
                                                                                                                       ggAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAcc                              <  2:21956/149‑1 (MQ=255)
                                                                                                                             tCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc                        >  4:495966/1‑149 (MQ=255)
                                                                                                                             tCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc                        >  4:1531683/1‑149 (MQ=255)
                                                                                                                               agacagaGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCATCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCaa                      >  1:719336/1‑149 (MQ=255)
                                                                                                                               agacagaGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCaa                      >  3:72580/1‑149 (MQ=255)
                                                                                                                                  cagaGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACga                   <  2:523168/149‑1 (MQ=255)
                                                                                                                                     agCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGagaa                <  4:72580/149‑1 (MQ=255)
                                                                                                                                        accaccaccGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAc             >  2:140219/1‑149 (MQ=255)
                                                                                                                                         ccaccaccGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCATCATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACg            >  1:1579259/1‑149 (MQ=255)
                                                                                                                                          caccaccGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACgg           >  3:262456/1‑149 (MQ=255)
                                                                                                                                           accaccGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGc          >  1:917842/1‑149 (MQ=255)
                                                                                                                                             caccGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGt        >  2:268192/1‑149 (MQ=255)
                                                                                                                                                 gTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAg    <  3:1531683/149‑1 (MQ=255)
                                                                                                                                                   ggggTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAGGa  <  4:84813/149‑1 (MQ=255)
                                                                                                                                                    gggTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAGGa  <  2:86865/148‑1 (MQ=255)
                                                                                                                                                    gggTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAGGa  >  1:86865/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGTCAAGGA  >  NC_000913/1900890‑1901185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: