Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,977,223:1 +A 7.5% intergenic (‑84/+43) motA ← / ← flhC proton conductor component of flagella motor/flagellar class II regulon transcriptional activator, with FlhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,977,2231.A 7.5% 650.7 / 51.4 161intergenic (‑84/+43)motA/flhCproton conductor component of flagella motor/flagellar class II regulon transcriptional activator, with FlhD
Reads supporting (aligned to +/- strand):  ref base . (74/75);  new base A (6/6);  total (80/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

Only 100 of 166 total aligned reads displayed.

CCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAA  >  NC_000913/1977075‑1977370
                                                                                                                                                     |                                                                                                                                                   
ccGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGt                                                                                                                                                      >  3:1297203/1‑149 (MQ=255)
ccGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGt                                                                                                                                                      <  3:1087419/149‑1 (MQ=255)
 cGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTa                                                                                                                                                    >  3:411395/1‑149 (MQ=255)
  ggTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAc                                                                                                                                                   <  2:813606/149‑1 (MQ=255)
     catcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCTATTGGGGCAAAAAAAAGCAGCGGTAcgtc                                                                                                                                                <  3:936108/149‑1 (MQ=255)
     catcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGTGGTAcgtc                                                                                                                                                <  4:1507643/149‑1 (MQ=255)
      atcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtcg                                                                                                                                               >  2:905644/1‑149 (MQ=255)
      atcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtcg                                                                                                                                               >  4:175655/1‑149 (MQ=255)
        caAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATCGGGGCAAAAAAAAGCAGCGGTaacgacgt                                                                                                                                             >  3:1155605/1‑142 (MQ=255)
         aaaTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTa                                                                                                                                            >  2:1248792/1‑149 (MQ=255)
         aaaTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTa                                                                                                                                            <  2:999668/149‑1 (MQ=255)
         aaaTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTa                                                                                                                                            >  1:1337009/1‑149 (MQ=255)
          aaTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTAc                                                                                                                                           <  3:123673/149‑1 (MQ=255)
           aTAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTAcc                                                                                                                                          <  2:1340688/149‑1 (MQ=255)
            tAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCg                                                                                                                                         >  4:352995/1‑149 (MQ=255)
                cgccgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgctgc                                                                                                                                     <  2:963801/149‑1 (MQ=255)
                     aaaaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAACGACGTACCgctgcttttt                                                                                                                                <  1:1423509/149‑5 (MQ=255)
                     aaaaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGaa                                                                                                                                <  4:299108/149‑1 (MQ=255)
                         cTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGtt                                                                                                                            <  4:1374962/149‑1 (MQ=255)
                           gTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgc                                                                                                                          >  1:303413/1‑149 (MQ=255)
                            tACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAACGACGTACCgctgctt                                                                                                                                   >  1:391988/1‑138 (MQ=255)
                                   aacaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCAcc                                                                                                                  <  1:991320/149‑1 (MQ=255)
                                      aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTa                                                                                                               >  3:826984/1‑149 (MQ=255)
                                      aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTa                                                                                                               >  2:211956/1‑149 (MQ=255)
                                         cAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTGACCGTATCa                                                                                                            <  3:607182/149‑1 (MQ=255)
                                          aGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAg                                                                                                           >  2:32284/1‑149 (MQ=255)
                                            gTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAc                                                                                                                                                   <  4:1192821/107‑1 (MQ=255)
                                              aaCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaa                                                                                                       >  3:1450487/1‑149 (MQ=255)
                                               aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                                                      >  2:72061/1‑149 (MQ=255)
                                               aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                                                      <  2:78419/149‑1 (MQ=255)
                                               aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                                                      >  3:1439243/1‑149 (MQ=255)
                                                  taataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAg                                                                                                   <  4:1479916/149‑1 (MQ=255)
                                                  taataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAg                                                                                                   >  4:839402/1‑149 (MQ=255)
                                                   aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAACGACGTACCgctgctttttt                                                                                                                               >  1:1050908/1‑115 (MQ=38)
                                                   aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAACGACGTACCgctgctttttt                                                                                                                               <  2:1050908/120‑6 (MQ=38)
                                                   aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                                                  <  2:665801/149‑1 (MQ=255)
                                                        gataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtcgt                                                                                                                                              >  4:1239977/1‑100 (MQ=255)
                                                         ataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTAc                                                                                            <  3:352995/149‑1 (MQ=255)
                                                         ataagCACGACATAATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTAc                                                                                            >  1:152564/1‑149 (MQ=255)
                                                             gCACGACATCATCCTTCCACTGTTGACCATAACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctc                                                                                        <  4:583109/149‑1 (MQ=255)
                                                             gCACGAAATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGGTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctc                                                                                        >  4:1421818/1‑149 (MQ=255)
                                                              cACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctct                                                                                       <  3:1529709/149‑1 (MQ=255)
                                                              cACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctct                                                                                       <  3:175655/149‑1 (MQ=255)
                                                              cACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctct                                                                                       <  2:1194450/149‑1 (MQ=255)
                                                                    atcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAg                                                                                                           <  2:617880/123‑1 (MQ=255)
                                                                    atcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAg                                                                                                           >  1:617880/1‑123 (MQ=255)
                                                                     tcatcCTTCCCCTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATc                                                                                <  2:575023/149‑1 (MQ=255)
                                                                     tcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATc                                                                                <  2:156940/149‑1 (MQ=255)
                                                                       atcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCca                                                                              >  4:1145249/1‑149 (MQ=255)
                                                                        tcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcag                                                                             <  3:545996/149‑1 (MQ=255)
                                                                          cTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagca                                                                           >  1:1349139/1‑149 (MQ=255)
                                                                              cACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctc                                                                                        <  1:769350/132‑1 (MQ=255)
                                                                              cACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctc                                                                                        >  2:769350/1‑132 (MQ=255)
                                                                               aCTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtgt                                                                      >  1:361726/1‑149 (MQ=255)
                                                                               aCTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtgt                                                                      <  2:306032/149‑1 (MQ=255)
                                                                                 tGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTgg                                                                    <  1:364166/149‑1 (MQ=255)
                                                                                    tGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTTGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGat                                                                 <  1:1214134/149‑1 (MQ=255)
                                                                                    tGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGat                                                                 <  1:232417/149‑1 (MQ=255)
                                                                                     gACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGata                                                                >  1:980177/1‑149 (MQ=255)
                                                                                     gACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGata                                                                <  3:1262654/149‑1 (MQ=255)
                                                                                      aCCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataa                                                               >  3:175001/1‑149 (MQ=255)
                                                                                        cATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataata                                                             >  1:1242533/1‑149 (MQ=255)
                                                                                        cATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataata                                                             >  1:681478/1‑149 (MQ=255)
                                                                                         aTGACAGGATGTTCAGTAGTCAGGCGTTAACGCGCGATTAGGGCAAAAAAAAGCAGCGGTACGCCGTTAACGCTGCTGGCATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCCGTCGTGGGATAatat                                                            >  2:1130639/1‑149 (MQ=255)
                                                                                          tGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATc                                                           <  2:1349139/149‑1 (MQ=255)
                                                                                          tGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAATTAAACAGCCTGTCCTCTCTGTTCAGCGAGCAGTTGTGGGATAATATc                                                           <  4:1538345/149‑1 (MQ=255)
                                                                                           gACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtc                                                                                                                                                <  1:1124784/63‑1 (MQ=255)
                                                                                            aCAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCgg                                                         <  2:43384/149‑1 (MQ=255)
                                                                                               ggATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAg                                                      <  2:361726/149‑1 (MQ=255)
                                                                                                gATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGTAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                                                  <  1:713384/104‑1 (MQ=255)
                                                                                                gATATTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTTAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAgg                                                     >  3:682499/1‑149 (MQ=255)
                                                                                                    ttCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgc                                                                                                                          <  4:437799/76‑1 (MQ=255)
                                                                                                    ttCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTc                                                 >  1:80244/1‑149 (MQ=255)
                                                                                                    ttCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTc                                                 >  1:173490/1‑149 (MQ=255)
                                                                                                        gtcgtcAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTggg                                             >  2:12652/1‑149 (MQ=255)
                                                                                                         tcgtcAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtcgt                                                                                                                                              >  2:442634/1‑51 (MQ=255)
                                                                                                         tcgtcAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtcgt                                                                                                                                              <  1:442634/51‑1 (MQ=255)
                                                                                                           gtcAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGaaa                                          >  3:1341825/1‑149 (MQ=255)
                                                                                                            tcAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTAAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAg                                         >  2:1506038/1‑149 (MQ=255)
                                                                                                              aGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGtt                                       >  3:334564/1‑149 (MQ=255)
                                                                                                                  gTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACg                                   >  4:894883/1‑149 (MQ=255)
                                                                                                                       cgcgcgATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCttt                              <  4:1341825/149‑1 (MQ=255)
                                                                                                                       cgcgcgATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAACTTTACGTCttt                              <  1:1183059/149‑1 (MQ=255)
                                                                                                                         cgcgATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCttttt                            <  3:264409/149‑1 (MQ=255)
                                                                                                                            gATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAGTGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACt                         >  3:1164585/1‑149 (MQ=255)
                                                                                                                            gATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACt                         >  2:919709/1‑149 (MQ=255)
                                                                                                                                   gCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGGCTGTActctct                                                                                       >  2:1552570/1‑80 (MQ=255)
                                                                                                                                   gCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActctct                                                                                       <  1:1552570/80‑1 (MQ=255)
                                                                                                                                   gCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCggg                  >  1:114768/1‑149 (MQ=255)
                                                                                                                                        aaaaaGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGACTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGc             <  1:124018/149‑1 (MQ=255)
                                                                                                                                         aaaaGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCg            <  1:12652/149‑1 (MQ=255)
                                                                                                                                            agcagcGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGtt         <  3:812431/149‑1 (MQ=255)
                                                                                                                                            agcagcGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGtt         <  4:759311/149‑1 (MQ=255)
                                                                                                                                             gcagcGGTAACGACGTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATc                                                           >  4:1029849/1‑98 (MQ=38)
                                                                                                                                             gcagcGGTAACGACGTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCtttt                             <  4:782427/128‑1 (MQ=255)
                                                                                                                                             gcagcGGTAACGACGTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTg        >  2:1239169/1‑149 (MQ=255)
                                                                                                                                             gcagcGGTAACGACGTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTg        >  1:1489958/1‑149 (MQ=255)
                                                                                                                                             gcagcGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTg        >  4:1331299/1‑149 (MQ=255)
                                                                                                                                               agcGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGAc      <  4:1264159/149‑1 (MQ=255)
                                                                                                                                               agcGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGAc      <  2:1311892/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAA  >  NC_000913/1977075‑1977370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: