Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,017,216:1 +A 7.3% intergenic (+24/‑117) udp → / → rmuC uridine phosphorylase/DNA recombination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,017,2161.A 7.3% 718.5 / 55.4 178intergenic (+24/‑117)udp/rmuCuridine phosphorylase/DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base . (86/79);  new base A (7/6);  total (93/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

Only 100 of 180 total aligned reads displayed.

CGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTA  >  NC_000913/4017069‑4017363
                                                                                                                                                    |                                                                                                                                                   
cGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGa                                                                                                                                                    >  1:645912/1‑149 (MQ=255)
       gTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTc                                                                                                                                             <  2:917104/149‑1 (MQ=255)
          gCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGc                                                                                                                                          <  2:751613/149‑1 (MQ=255)
            gggTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCt                                                                                                                                        >  1:671028/1‑149 (MQ=255)
             ggTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtt                                                                                                                                       >  4:736924/1‑149 (MQ=255)
              gtgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGCCttc                                                                                                                                      <  4:236313/149‑2 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                     <  1:77040/149‑1 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                     >  4:131106/1‑149 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                     <  4:1025894/149‑1 (MQ=255)
                 ttATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGt                                                                                                                                   <  3:85936/149‑1 (MQ=255)
                  tATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTa                                                                                                                                  >  3:609176/1‑149 (MQ=255)
                   aTCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAt                                                                                                                                 >  4:355255/1‑149 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                >  2:368124/1‑149 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                >  1:950027/1‑149 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                <  2:950027/149‑1 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                >  4:344998/1‑149 (MQ=255)
                      gTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtttt                                                                                                                              <  1:884418/149‑1 (MQ=255)
                       ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAttttt                                                                                                                             <  3:776253/149‑1 (MQ=255)
                       ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAttttt                                                                                                                             >  2:493917/1‑149 (MQ=255)
                        tAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTg                                                                                                                            <  3:355255/149‑1 (MQ=255)
                           ccGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGt                                                                                                                         >  4:507800/1‑149 (MQ=255)
                                   aGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTc                                                                                                                 >  3:1029217/1‑149 (MQ=255)
                                    gCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGCCttc                                                                                                                                      >  4:578995/1‑126 (MQ=255)
                                           aTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                     <  1:1074934/121‑1 (MQ=255)
                                            tCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtttt                                                                                                                              <  2:1383963/127‑1 (MQ=255)
                                            tCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAAt                                                                                                        >  4:1037395/1‑149 (MQ=255)
                                            tCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAAt                                                                                                        <  4:1529171/149‑1 (MQ=255)
                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGc                                                                                                      <  1:336741/149‑1 (MQ=255)
                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCAGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGc                                                                                                      <  4:1130052/149‑1 (MQ=255)
                                                  aTGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCttt                                                                                                  <  3:1221890/149‑1 (MQ=255)
                                                       gagaCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAAc                                                                                             <  3:736924/149‑1 (MQ=255)
                                                       gagaCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAAc                                                                                             <  4:1235119/149‑1 (MQ=255)
                                                              tGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                      <  1:691403/149‑1 (MQ=255)
                                                              cgAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                      <  3:138715/148‑1 (MQ=255)
                                                               gAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCCACTGGACGtt                                                                                     >  2:624389/1‑149 (MQ=255)
                                                                aaacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACCGGACGttt                                                                                    <  4:330449/149‑1 (MQ=255)
                                                                   caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTa                                                                                 <  2:1537794/149‑1 (MQ=255)
                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGcc                                                                                                     >  3:989997/1‑128 (MQ=255)
                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTAc                                                                                >  1:761563/1‑149 (MQ=255)
                                                                     aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACa                                                                               >  2:575118/1‑149 (MQ=255)
                                                                       ccGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                                    <  4:945004/142‑1 (MQ=255)
                                                                        cGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                            >  4:220393/1‑149 (MQ=255)
                                                                         gAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGcac                                                                           <  3:507800/149‑1 (MQ=255)
                                                                              ccATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTc                                                                      >  3:1198516/1‑149 (MQ=255)
                                                                                  gCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTAtt                                                                  <  4:616387/149‑1 (MQ=255)
                                                                                  gCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTAtt                                                                  <  2:1154053/149‑1 (MQ=255)
                                                                                      tGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtgt                                                              >  1:1523722/1‑149 (MQ=255)
                                                                                        aaaaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGc                                                            <  2:1426770/149‑1 (MQ=255)
                                                                                         aaaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCACAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCg                                                           <  3:131106/149‑1 (MQ=255)
                                                                                          aaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCgg                                                          >  1:1111232/1‑149 (MQ=255)
                                                                                          aaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCgg                                                          <  2:960625/149‑1 (MQ=255)
                                                                                               tggtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAg                                                     >  1:1175608/1‑149 (MQ=255)
                                                                                                 gtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgtt                                                   >  4:4861/1‑149 (MQ=255)
                                                                                                  tggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttg                                                  >  3:1487985/1‑149 (MQ=255)
                                                                                                   ggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgt                                                 >  1:149470/1‑149 (MQ=255)
                                                                                                    gAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgtt                                                <  3:1252903/149‑1 (MQ=255)
                                                                                                    gAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgtt                                                <  3:4861/149‑1 (MQ=255)
                                                                                                     aaGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                                    <  2:1125212/112‑1 (MQ=255)
                                                                                                          gcgcGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCa                                          <  2:172570/149‑1 (MQ=255)
                                                                                                               tcgtcTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggagg                                     >  3:88735/1‑149 (MQ=255)
                                                                                                                   ctgctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGtt                                 <  1:183999/149‑1 (MQ=255)
                                                                                                                   ctgctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGtt                                 <  3:585474/149‑1 (MQ=255)
                                                                                                                    tgctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtg                                >  2:594965/1‑149 (MQ=255)
                                                                                                                      ctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgtg                              <  2:671028/149‑1 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                             <  4:869526/149‑1 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                             >  4:1379259/1‑149 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                             >  2:1031048/1‑149 (MQ=255)
                                                                                                                         tAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGACGCGTTGTGGAt                           <  3:916725/149‑1 (MQ=255)
                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCGACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAttt                         <  1:1403070/149‑1 (MQ=255)
                                                                                                                             tctcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGTGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtct                       >  1:1288746/1‑149 (MQ=255)
                                                                                                                             tctcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtct                       <  1:1150502/149‑1 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCa                     >  2:213033/1‑149 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCa                     >  2:207135/1‑149 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCa                     >  1:1223115/1‑149 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCa                     >  2:655138/1‑149 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGGATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgtt                                                <  4:888470/122‑1 (MQ=255)
                                                                                                                                cttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCaa                    <  4:640559/149‑1 (MQ=255)
                                                                                                                                cttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCaa                    <  1:575118/149‑1 (MQ=255)
                                                                                                                                cttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCaa                    <  3:460358/149‑1 (MQ=255)
                                                                                                                                 ttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAAt                   >  4:587607/1‑149 (MQ=255)
                                                                                                                                 ttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAAt                   >  3:656449/1‑149 (MQ=255)
                                                                                                                                 ttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAAt                   <  3:572684/149‑1 (MQ=255)
                                                                                                                                    tcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGTGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCAt                <  2:1277561/149‑1 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                      <  2:1207087/125‑1 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                      >  1:1207087/1‑125 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATgg              >  4:1365573/1‑149 (MQ=255)
                                                                                                                                        gTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGtt            >  2:734792/1‑149 (MQ=255)
                                                                                                                                           tGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCt                                                                                                    <  3:1109422/58‑1 (MQ=255)
                                                                                                                                           aaaaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc         >  1:447455/3‑149 (MQ=255)
                                                                                                                                            gAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                      <  3:776068/119‑1 (MQ=255)
                                                                                                                                            gAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                      >  4:776068/1‑119 (MQ=255)
                                                                                                                                            aaaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTa                                                                    >  1:12253/2‑89 (MQ=255)
                                                                                                                                            aaaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTa                                                                    <  2:12253/88‑1 (MQ=255)
                                                                                                                                             aaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCag                                         >  2:308622/1‑115 (MQ=255)
                                                                                                                                             aaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCag                                         <  1:308622/115‑1 (MQ=255)
                                                                                                                                             aaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGc       >  3:1310104/1‑149 (MQ=255)
                                                                                                                                             aaGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGc       >  1:1519232/1‑149 (MQ=255)
                                                                                                                                              aGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCa      >  4:660487/1‑149 (MQ=255)
                                                                                                                                               ggCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACATGTCTATTTGGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAACCATGGTTTACGCAg     <  2:255600/149‑1 (MQ=255)
                                                                                                                                                  cGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTa  >  3:609376/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTA  >  NC_000913/4017069‑4017363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: