Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,370,026 | T→A | 6.1% | intergenic (+23/‑190) | pspE → / → ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,370,026 | 0 | T | A | 6.1% | 503.6 / 17.7 | 147 | intergenic (+23/‑190) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
Reads supporting (aligned to +/- strand): ref base T (74/63); new base A (6/3); total (81/66) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.13e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 |
Only 100 of 151 total aligned reads displayed.
AAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAG > NC_000913/1369879‑1370174 | aaGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt > 1:308231/1‑149 (MQ=255) aaGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCtt < 4:1392297/149‑1 (MQ=255) aGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTa < 3:98700/149‑1 (MQ=255) gtgtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAt < 4:729355/149‑1 (MQ=255) gtgtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAt < 4:565479/149‑1 (MQ=255) tgtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATg > 4:720828/1‑149 (MQ=255) gtATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATgc < 3:864078/149‑1 (MQ=255) aTTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATgcgc > 2:825897/1‑149 (MQ=255) ttGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGcc > 4:472372/1‑149 (MQ=255) gCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t > 1:1315245/1‑149 (MQ=255) cAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tt < 3:1186112/149‑1 (MQ=255) aaTGCCGGACGCCAGATAGGGCAAGCAGAAGATATCCTTAGCGAGATGGGCTATACCCACGATGAGAACGCCGGTGGCCTGAAAAACATCGCAATGCCGAAGGTCAAAGGCTAAAAGAATTCACCATGAGCGGCGCGTACGAGCCG‑‑ttt > 2:1314579/1‑149 (MQ=255) tGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑ttttt > 1:359181/1‑149 (MQ=255) ccGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑ttttttt < 3:90151/149‑1 (MQ=255) ggACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑ttttttttt > 3:473908/1‑149 (MQ=255) tCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCacac < 2:575267/149‑1 (MQ=255) cAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACAcc < 4:288880/149‑1 (MQ=255) ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTa > 1:372551/1‑149 (MQ=255) gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTAt > 3:48821/1‑149 (MQ=255) gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTAt > 4:805953/1‑149 (MQ=255) aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTAttt > 3:169001/1‑149 (MQ=255) gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTAc > 1:1581395/1‑149 (MQ=255) aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATgcgc > 4:338717/1‑118 (MQ=255) aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATgcgc < 3:338717/118‑1 (MQ=255) aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTAttt > 3:941401/1‑145 (MQ=255) aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACAt > 4:1035089/1‑149 (MQ=255) aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATa > 2:470013/1‑149 (MQ=255) aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATa > 3:792425/1‑149 (MQ=255) aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAAACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATa < 2:967532/149‑1 (MQ=255) tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAg < 1:669386/149‑1 (MQ=255) tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAg > 1:1397608/1‑149 (MQ=255) ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGa > 2:1139140/1‑149 (MQ=255) ttAGCGAGATGGGATATACCCACGTTTAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTACGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGatt > 1:956870/1‑149 (MQ=255) ttAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAAGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGatt > 4:1218513/1‑149 (MQ=255) cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTTTCTTTTACACCTTATTTACATCCCCATAAattat > 4:945808/1‑149 (MQ=255) agaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGc > 2:1370978/1‑149 (MQ=255) gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc > 1:832645/1‑149 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTTTTTCTGTAAACCCTATTTTCCTTCCCCATAGTTTTTTTGCTTCAGCTccaaa > 4:900303/1‑145 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTca > 3:1338660/1‑105 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa > 1:110313/1‑149 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa > 4:79433/1‑149 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa > 2:1041577/1‑149 (MQ=255) cACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt > 4:380051/1‑149 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATAc < 4:758437/149‑1 (MQ=255) gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGc < 3:960032/132‑1 (MQ=255) gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGc > 4:960032/1‑132 (MQ=255) ttGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc < 1:1121712/149‑1 (MQ=255) ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt < 2:1315245/149‑1 (MQ=255) ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt < 1:746479/149‑1 (MQ=255) ggTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg < 2:846246/149‑1 (MQ=255) ggTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg < 3:217541/149‑1 (MQ=255) cggAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGGGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCCCAAATACGCTTTTTCCCTGCTaaaaa > 2:474197/3‑149 (MQ=255) tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa > 3:978596/1‑149 (MQ=255) tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa > 2:185475/1‑149 (MQ=255) tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa > 4:32278/1‑149 (MQ=255) gAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑ttttttttt > 1:932139/1‑75 (MQ=255) aGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAt < 2:956870/149‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTccc < 4:921835/131‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTcc > 1:1003063/1‑149 (MQ=255) tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGa > 3:1202505/1‑143 (MQ=255) tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCt < 4:1348366/149‑1 (MQ=255) cGCAATGCCGAAGGTC‑AAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc < 4:765761/149‑1 (MQ=255) gCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc < 1:594950/149‑1 (MQ=255) cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg > 3:287584/1‑149 (MQ=255) cGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACt > 2:1464132/1‑149 (MQ=255) cGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa > 4:330249/1‑149 (MQ=255) aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa < 4:169001/149‑1 (MQ=255) aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa < 2:830562/149‑1 (MQ=255) gTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAcc > 4:196741/1‑149 (MQ=255) tCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc > 4:940188/1‑149 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTCTTTCCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt > 1:1029069/1‑149 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt < 4:287584/149‑1 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt > 2:1368888/1‑149 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc > 3:637940/1‑134 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc < 4:637940/134‑1 (MQ=255) aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt < 3:919427/149‑1 (MQ=255) aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtg < 1:1370978/149‑1 (MQ=255) aGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTGCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt < 4:978596/149‑1 (MQ=255) ggTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgtg < 3:1218513/149‑1 (MQ=255) aaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa > 3:646877/1‑149 (MQ=255) aaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa < 2:561632/149‑1 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt < 3:1333529/149‑1 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCACCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCTCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt > 4:16086/1‑149 (MQ=255) aGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAtt < 3:834203/149‑1 (MQ=255) gAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAtt < 1:377889/149‑1 (MQ=255) aaTTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGa < 2:856214/149‑1 (MQ=255) ttCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa > 4:113596/1‑149 (MQ=255) ttCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACa > 4:203713/1‑149 (MQ=255) aCCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGtt > 4:411048/1‑149 (MQ=255) cATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTa > 1:727389/1‑149 (MQ=255) aaaaaacggcgCATAAGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACa < 3:773800/138‑1 (MQ=255) gAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAttt > 4:902941/1‑149 (MQ=255) aaacggcgCATAAGCGCCGTTTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 4:1334359/4‑149 (MQ=255) aaacggcgCATAAGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAttt > 1:1245788/4‑149 (MQ=255) aaacggcgCATAAGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGAATCTTTGCGTCCGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCTTGACTAAAAACCCTTCGCTCAATTGACAGTTTAttt > 1:8696/4‑149 (MQ=255) acggcgCATAAGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt > 2:1484640/2‑127 (MQ=255) acggcgCATAAGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt < 1:1484640/126‑1 (MQ=255) cggcgCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCt < 2:252995/149‑1 (MQ=255) ggcgCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg > 3:1055378/1‑149 (MQ=255) ttATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGag > 3:1492105/1‑149 (MQ=255) | AAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAG > NC_000913/1369879‑1370174 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |