Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,966 T→A 5.8% intergenic (‑39/+18) ralR ← / ← recT Rac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9660TA 5.8% 586.1 / 21.2 171intergenic (‑39/+18)ralR/recTRac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base T (74/87);  new base A (6/4);  total (80/91)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01

Only 100 of 173 total aligned reads displayed.

TCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAT  >  NC_000913/1413820‑1414113
                                                                                                                                                  |                                                                                                                                                   
tCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTa                                                                                                                                                   <  1:599192/149‑1 (MQ=255)
       tCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGcc                                                                                                                                            <  1:1171048/149‑1 (MQ=255)
       tCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATAGTTCAAGTTGGCGGTGTAATGCACCGcc                                                                                                                                            <  3:820947/149‑1 (MQ=255)
        cGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCa                                                                                                                                           <  2:349702/149‑1 (MQ=255)
         gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGAAGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaa                                                                                                                                          <  1:279636/149‑2 (MQ=255)
               gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGaa                                                                                                                                    <  1:1423454/149‑1 (MQ=255)
                ttACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAat                                                                                                                                   >  4:1095314/1‑149 (MQ=255)
                      ttCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                             >  4:1022956/1‑149 (MQ=255)
                           tCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGa                                                                                                                        >  4:52295/1‑149 (MQ=255)
                           gcGGTAATATCCTGAGATGGCATTCACCGTTACTGATGGACAACCACAAAATGGACAGGGTTTAACATTGTCATATCTCATAATTTTGCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGa                                                                                                                        >  4:369913/2‑149 (MQ=255)
                            cGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGaa                                                                                                                       <  1:742259/149‑1 (MQ=255)
                                 atatCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACCCCGCCAGGCTGAATTATTCCTCTGAATTATc                                                                                                                  <  4:1212877/149‑1 (MQ=255)
                                 atatCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                                                                                  <  2:873982/149‑1 (MQ=255)
                                 atatCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                                                                                  <  4:1527192/149‑1 (MQ=255)
                                    tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                               <  3:372103/149‑1 (MQ=255)
                                    tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                               >  3:1433860/1‑149 (MQ=255)
                                     ccTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt                                                                                                              >  1:1191649/1‑149 (MQ=255)
                                      cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                             >  2:1145444/1‑133 (MQ=255)
                                      cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                             <  1:1145444/133‑1 (MQ=255)
                                       tGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTaca                                                                                                                                                 >  4:1389739/1‑112 (MQ=255)
                                       tGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTaca                                                                                                                                                 <  3:1389739/112‑1 (MQ=255)
                                         aaaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                          <  3:137120/149‑1 (MQ=255)
                                          aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                         <  1:1235952/149‑1 (MQ=255)
                                           aTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTg                                                                                                        >  4:1110146/1‑149 (MQ=255)
                                             ggCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTa                                                                                                      >  4:661036/1‑149 (MQ=255)
                                                tttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                          >  3:1103144/1‑142 (MQ=255)
                                                  tCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                 <  3:1022956/149‑1 (MQ=255)
                                                    aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTac                                                                                                                                                  >  1:389184/1‑98 (MQ=255)
                                                    aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTac                                                                                                                                                  <  2:389184/98‑1 (MQ=255)
                                                    aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCg                                                                                               <  4:1001624/149‑1 (MQ=255)
                                                    aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCg                                                                                               >  1:695748/1‑149 (MQ=255)
                                                        ttACTGATGGACAATCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTa                                                                                           >  3:147417/1‑149 (MQ=255)
                                                         tACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTACTCCCCGGTTaa                                                                                          <  2:186280/149‑1 (MQ=255)
                                                           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATCATCGATTACACTGTATTCCCCGGTTAATa                                                                                        <  1:976306/149‑1 (MQ=255)
                                                            tGATGGACAACCACAAAATGGATATGGTTTAACATTGTCATATCTCATAATGTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATAc                                                                                       >  2:1282640/1‑149 (MQ=255)
                                                            tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATAc                                                                                       >  3:543942/1‑149 (MQ=255)
                                                             gATGGACAACCACCAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACa                                                                                      <  4:994799/149‑1 (MQ=255)
                                                             gATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACa                                                                                      <  2:1531265/149‑1 (MQ=255)
                                                             gATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACa                                                                                      >  1:963803/1‑149 (MQ=255)
                                                              aTGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACag                                                                                     >  1:1556913/1‑149 (MQ=255)
                                                               tGGACAATCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                    <  4:147417/149‑1 (MQ=255)
                                                               tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATCCaga                                                                                    <  2:949852/149‑1 (MQ=255)
                                                               tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                    <  1:109107/149‑1 (MQ=255)
                                                                ggACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACCCTGTATTCCCCGGTTAATACagag                                                                                   <  2:867090/149‑1 (MQ=255)
                                                                    aaCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                                                                               >  1:1417302/1‑149 (MQ=255)
                                                                    aaCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                                                                               >  3:422474/1‑149 (MQ=255)
                                                                    aaCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                                                                               <  2:1244109/149‑1 (MQ=255)
                                                                     aCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATc                                                                              <  2:574088/149‑1 (MQ=255)
                                                                       cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                                                                            >  2:784076/1‑149 (MQ=255)
                                                                       cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                                                                            >  4:1058658/1‑149 (MQ=255)
                                                                          aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                         <  2:695748/149‑1 (MQ=255)
                                                                          aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                         <  4:1178403/149‑1 (MQ=255)
                                                                          aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                         <  2:354457/149‑1 (MQ=255)
                                                                                 aCATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                  <  3:661036/149‑1 (MQ=255)
                                                                                  cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAtt                                                                 >  1:846472/1‑149 (MQ=255)
                                                                                     ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTc                                                              <  1:544200/149‑1 (MQ=255)
                                                                                          aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                  >  2:993632/1‑140 (MQ=255)
                                                                                             aTTGTCATGTCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTTCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTc                                                      >  1:1040525/1‑149 (MQ=255)
                                                                                             aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTc                                                      >  1:1333895/1‑149 (MQ=255)
                                                                                              ttGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc                                                     <  2:1417302/149‑1 (MQ=255)
                                                                                                gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCtt                                                   <  4:1471487/149‑1 (MQ=255)
                                                                                                gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTACCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCtt                                                   <  3:369913/149‑1 (MQ=255)
                                                                                                 tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctc                                                                                                                           <  4:695630/76‑1 (MQ=255)
                                                                                                 tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCttt                                                  >  3:123429/1‑149 (MQ=255)
                                                                                                   atatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTc                                                >  1:1059084/1‑149 (MQ=255)
                                                                                                      tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                    >  1:664366/1‑110 (MQ=255)
                                                                                                      tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATc                                             >  1:1115715/1‑149 (MQ=255)
                                                                                                      tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATc                                             <  3:1110146/149‑1 (MQ=255)
                                                                                                       ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                        >  3:141252/1‑105 (MQ=255)
                                                                                                        tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCa                                           <  3:607633/149‑1 (MQ=255)
                                                                                                         cATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAt                                          >  2:998912/1‑149 (MQ=255)
                                                                                                          aTAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAtt                                         >  3:893508/1‑149 (MQ=255)
                                                                                                            aaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGa                                       <  3:552215/149‑1 (MQ=255)
                                                                                                             aTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGAt                                      <  4:478628/149‑1 (MQ=255)
                                                                                                              tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                    <  2:679027/102‑1 (MQ=255)
                                                                                                                    tcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAtt                                                                 >  2:1333625/1‑115 (MQ=255)
                                                                                                                       tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCt                            <  4:1433860/149‑1 (MQ=255)
                                                                                                                        aaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                    <  3:1059849/92‑1 (MQ=255)
                                                                                                                        aaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTg                           <  3:259014/149‑1 (MQ=255)
                                                                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTGTCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                          >  2:1582143/1‑149 (MQ=255)
                                                                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                          >  3:11949/1‑149 (MQ=255)
                                                                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                          >  1:506595/1‑149 (MQ=255)
                                                                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                          >  4:240912/1‑149 (MQ=255)
                                                                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                          >  3:1431792/1‑149 (MQ=255)
                                                                                                                                tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCaa                   >  4:776557/1‑149 (MQ=255)
                                                                                                                                tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCaa                   >  2:36482/1‑149 (MQ=255)
                                                                                                                                 ttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAt                  >  1:657007/1‑149 (MQ=255)
                                                                                                                                 ttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAt                  <  2:1067587/149‑1 (MQ=255)
                                                                                                                                 ttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAt                  <  4:575000/149‑1 (MQ=255)
                                                                                                                                    aaGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGa               >  1:254390/1‑149 (MQ=255)
                                                                                                                                     aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGAt              <  2:254390/149‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATAc            <  1:1007702/149‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATAc            <  2:681105/149‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATAc            >  3:483133/1‑149 (MQ=255)
                                                                                                                                        tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATAc            >  4:1451124/1‑148 (MQ=255)
                                                                                                                                        tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACg           >  4:545222/1‑149 (MQ=255)
                                                                                                                                        tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACg           >  2:455468/1‑149 (MQ=255)
                                                                                                                                             gTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCaa      >  2:433057/1‑149 (MQ=255)
                                                                                                                                                cATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAata   >  1:1241184/1‑149 (MQ=255)
                                                                                                                                                 aTTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAatat  >  4:1238479/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAT  >  NC_000913/1413820‑1414113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: