Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,498,892 A→T 7.5% intergenic (+17/‑46) ydcH → / → rimL DUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,498,8920AT 7.5% 506.6 / 24.5 146intergenic (+17/‑46)ydcH/rimLDUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (74/61);  new base T (3/8);  total (77/69)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 155 total aligned reads displayed.

AATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGT  >  NC_000913/1498746‑1499040
                                                                                                                                                  |                                                                                                                                                    
     ccAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggc                                                                                                                                               <  1:679677/149‑1 (MQ=255)
       aGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggcgg                                                                                                                                             >  3:845902/1‑149 (MQ=255)
        gAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggcggc                                                                                                                                            <  3:847409/149‑1 (MQ=255)
          aaggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAGAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCtt                                                                                                                                          <  2:1311126/149‑1 (MQ=255)
           aggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCtta                                                                                                                                         <  2:156404/149‑2 (MQ=255)
           aggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTGAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTggcggcgta                                                                                                                                         <  1:388730/149‑4 (MQ=255)
            ggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttt                                                                                                                                        >  4:789772/1‑149 (MQ=255)
             gaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttttt                                                                                                                                       >  2:1217122/1‑149 (MQ=255)
             gaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttttt                                                                                                                                       <  4:532927/149‑1 (MQ=255)
              aaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttttt                                                                                                                                      <  3:789772/149‑1 (MQ=255)
                         ggTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCAt                                                                                                                           <  2:1335787/149‑1 (MQ=255)
                          gTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttttt                                                                                                                                       >  2:1383059/1‑136 (MQ=255)
                          gTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttttt                                                                                                                                       <  1:1383059/136‑1 (MQ=255)
                             gAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGAt                                                                                                                       <  3:659611/149‑1 (MQ=255)
                              aGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGAtt                                                                                                                      <  2:544962/149‑1 (MQ=255)
                                ggTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGa                                                                                                                    <  2:1232600/149‑1 (MQ=255)
                                 gTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGAc                                                                                                                   <  2:117748/149‑1 (MQ=255)
                                  tACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACt                                                                                                                  >  4:1184861/1‑149 (MQ=255)
                                  tACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACt                                                                                                                  >  3:188054/1‑149 (MQ=255)
                                   aCAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTg                                                                                                                 <  1:1119936/149‑1 (MQ=255)
                                          ggAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATaa                                                                                                          >  3:463064/1‑149 (MQ=255)
                                          ggAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATaa                                                                                                          >  2:723350/1‑149 (MQ=255)
                                          ggAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATaa                                                                                                          <  1:723350/149‑1 (MQ=255)
                                            aaGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGa                                                                                                        <  1:871035/149‑1 (MQ=255)
                                               tGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGa                                                                                                     <  4:830968/149‑1 (MQ=255)
                                                ggTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGAc                                                                                                    <  2:53363/149‑1 (MQ=255)
                                                  tCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTg                                                                                                  >  2:1509818/1‑149 (MQ=255)
                                                     gCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTaacaac                                                                                                                                <  1:508077/116‑1 (MQ=255)
                                                     gCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGaaa                                                                                               <  3:365962/149‑1 (MQ=255)
                                                      cATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAAc                                                                                              >  1:1557496/1‑149 (MQ=255)
                                                      cATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAAc                                                                                              <  4:216006/149‑1 (MQ=255)
                                                          aaaaaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATa                                                                                          >  3:1308911/1‑149 (MQ=255)
                                                             aaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATaaaa                                                                                       <  3:771471/149‑1 (MQ=255)
                                                               aCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGga                                                                                                               >  2:601178/1‑123 (MQ=255)
                                                               aCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGt                                                                                     >  3:270952/1‑149 (MQ=255)
                                                                 aaaaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATg                                                                                                                          >  3:641271/1‑110 (MQ=255)
                                                                 aaaaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATg                                                                                                                          <  4:641271/110‑1 (MQ=255)
                                                                  aaaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAg                                                                                  >  2:1413877/1‑149 (MQ=255)
                                                                   aaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGc                                                                                 <  1:1413877/149‑1 (MQ=255)
                                                                     gCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCttag                                                                                                                                        <  2:1078649/92‑3 (MQ=255)
                                                                     gCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCttag                                                                                                                                        >  1:1078649/1‑90 (MQ=255)
                                                                     gCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCAt                                                                                                                           <  4:1319469/105‑1 (MQ=255)
                                                                      cTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGaa                                                                              >  4:920674/1‑149 (MQ=255)
                                                                           gTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACt                                                                         <  4:389341/149‑1 (MQ=255)
                                                                           gTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTATACAACTGCATGGATTGACTGGAGATAAGCTGACTGAAACGATAAAAGTAAGCGAAGCACt                                                                         <  2:797223/149‑1 (MQ=255)
                                                                            ttAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttttt                                                                                                                                      >  2:1513001/1‑87 (MQ=255)
                                                                            ttAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttttt                                                                                                                                      <  1:1513001/87‑1 (MQ=255)
                                                                            ttAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACtt                                                                        <  4:1274904/149‑1 (MQ=255)
                                                                            ttAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACtt                                                                        <  4:1399411/149‑1 (MQ=255)
                                                                            ttAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACtt                                                                        >  4:370039/1‑149 (MQ=255)
                                                                            atAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACtt                                                                        >  2:467439/2‑149 (MQ=255)
                                                                               aaaGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGaa                                                                     >  2:1117870/1‑149 (MQ=255)
                                                                               aaaGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGaa                                                                     <  3:1013769/149‑1 (MQ=255)
                                                                                   atgagatgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTAc                                                                 <  3:119156/149‑1 (MQ=255)
                                                                                          gCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCAt                                                                                                                           <  1:602457/84‑1 (MQ=255)
                                                                                             cAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGc                                                       >  3:569397/1‑149 (MQ=255)
                                                                                              aaaaTCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCa                                                      >  1:820700/1‑149 (MQ=255)
                                                                                              aaaaTCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCa                                                      >  2:60633/1‑149 (MQ=255)
                                                                                                aaTCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGa                                                    <  4:845902/149‑1 (MQ=255)
                                                                                                     tGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGa                                                                                                        <  1:557634/92‑1 (MQ=255)
                                                                                                     tGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATc                                               >  2:676148/1‑149 (MQ=255)
                                                                                                        agcagGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACg                                            <  2:198889/149‑1 (MQ=255)
                                                                                                        agcagGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACg                                            >  3:545556/1‑149 (MQ=255)
                                                                                                           agGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCa                                         >  4:742045/1‑149 (MQ=255)
                                                                                                             gagaGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCaaa                                       <  2:1557496/149‑1 (MQ=255)
                                                                                                              agagCGTCAAAGCGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAAc                                      <  1:1261881/149‑1 (MQ=255)
                                                                                                               gagCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAAcc                                     >  2:1332217/1‑149 (MQ=255)
                                                                                                                   gTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGtt                                                         <  1:831723/125‑1 (MQ=255)
                                                                                                                    tCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCttt                                >  2:493360/1‑149 (MQ=255)
                                                                                                                    tCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCttt                                >  4:44227/1‑149 (MQ=255)
                                                                                                                     cAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTa                               <  3:44227/149‑1 (MQ=255)
                                                                                                                            gtgtAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTa                        <  4:944580/149‑1 (MQ=255)
                                                                                                                              gtAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATa                                                                                          >  1:114137/1‑81 (MQ=255)
                                                                                                                              gtAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATa                                                                                          <  2:114137/81‑1 (MQ=255)
                                                                                                                              gtAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAAt                      >  1:663205/1‑149 (MQ=255)
                                                                                                                               tAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATc                     >  1:893654/1‑149 (MQ=255)
                                                                                                                                 aaCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTg                   >  3:1177620/1‑149 (MQ=255)
                                                                                                                                 aaCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTg                   >  3:20523/1‑149 (MQ=255)
                                                                                                                                 aaCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTg                   >  3:389892/1‑149 (MQ=255)
                                                                                                                                  aCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAGCTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGt                  <  3:958348/149‑1 (MQ=255)
                                                                                                                                  aCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGt                          >  3:285792/1‑141 (MQ=255)
                                                                                                                                  aCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGt                          <  4:285792/141‑1 (MQ=255)
                                                                                                                                    tttCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGAc                                                                                                    >  3:474461/1‑65 (MQ=255)
                                                                                                                                    tttCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGAc                                                                                                    <  4:474461/65‑1 (MQ=255)
                                                                                                                                    tttCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACAGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTaa                >  4:903496/1‑149 (MQ=255)
                                                                                                                                      tCCGAAGCCGCCAGCCAGGCGGCTATTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTaaaa              >  4:1057990/1‑149 (MQ=255)
                                                                                                                                       ccTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTaaaaa             <  2:91035/149‑1 (MQ=255)
                                                                                                                                        cTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAAt            >  3:924352/1‑149 (MQ=255)
                                                                                                                                        cTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAAt            >  1:1502277/1‑149 (MQ=255)
                                                                                                                                        cTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAAt            >  1:1045576/1‑149 (MQ=255)
                                                                                                                                         tAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATa           >  4:722019/1‑149 (MQ=255)
                                                                                                                                         tAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATa           >  4:784831/1‑149 (MQ=255)
                                                                                                                                         tAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGATAATCTGTAAAAATa           >  3:709053/1‑149 (MQ=255)
                                                                                                                                         aaaGCCGCCTGGCTGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATa           >  1:711360/2‑149 (MQ=255)
                                                                                                                                          aaGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaa          >  2:574551/1‑149 (MQ=255)
                                                                                                                                           aGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaaa         <  1:332179/149‑1 (MQ=255)
                                                                                                                                            ggcggcagccagGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTAAATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaaaa        <  2:780352/144‑1 (MQ=255)
                                                                                                                                                ccagccagGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTg    <  3:1254338/149‑1 (MQ=255)
                                                                                                                                                ccagccagGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTg    <  1:1317567/149‑1 (MQ=255)
                                                                                                                                                 cagccagGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTgg   <  4:491264/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGT  >  NC_000913/1498746‑1499040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: