Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,747,027 T→A 9.0% intergenic (+22/‑104) ydhR → / → ydhS putative monooxygenase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,747,0270TA 9.0% 522.3 / 31.2 155intergenic (+22/‑104)ydhR/ydhSputative monooxygenase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (72/69);  new base A (5/9);  total (77/78)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 156 total aligned reads displayed.

AGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTA  >  NC_000913/1746880‑1747174
                                                                                                                                                   |                                                                                                                                                   
aGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTa                                                                                                                                                    <  4:1374535/149‑1 (MQ=255)
aGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTa                                                                                                                                                    >  2:877122/1‑149 (MQ=255)
     ccTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGcc                                                                                                                                               <  1:1316862/149‑1 (MQ=255)
         gCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCttt                                                                                                                                           >  2:1432037/1‑149 (MQ=255)
         gCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                           <  3:191439/149‑2 (MQ=255)
         gCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                           <  1:442476/149‑2 (MQ=255)
          ccTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttga                                                                                                                                          <  2:1251994/149‑3 (MQ=255)
            tATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCa                                                                                                                                        >  3:61628/1‑149 (MQ=255)
              tCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATc                                                                                                                                      >  1:1203888/1‑149 (MQ=255)
                 ggAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCt                                                                                                                                   >  3:455052/1‑149 (MQ=255)
                 ggAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCt                                                                                                                                   >  1:976305/1‑149 (MQ=255)
                 ggAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCt                                                                                                                                   <  3:1198921/149‑1 (MQ=255)
                  gAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTg                                                                                                                                  >  4:1101568/1‑149 (MQ=255)
                            cTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAAttt                                                                                                                        <  1:877122/149‑1 (MQ=255)
                                     tGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGt                                                                                                                                 >  1:252258/1‑131 (MQ=255)
                                     tGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGt                                                                                                                                 <  2:252258/131‑1 (MQ=255)
                                         aaaTCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTaaa                                                                                                           <  3:817273/149‑1 (MQ=255)
                                             ctcGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtc                                                                                                       <  3:1101568/149‑1 (MQ=255)
                                              tcGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtct                                                                                                      >  1:186486/1‑149 (MQ=255)
                                               cGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGTCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtctc                                                                                                     <  2:1063933/149‑1 (MQ=255)
                                               cGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtctc                                                                                                     <  2:611260/149‑1 (MQ=255)
                                                 gCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATATCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTGAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCaa                                                                                                   >  4:1003717/1‑149 (MQ=255)
                                                         gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTa                                                                                                             <  4:88445/131‑1 (MQ=255)
                                                         gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTa                                                                                                             >  3:88445/1‑131 (MQ=255)
                                                            gTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCACCCAAATTCATc                                                                                        <  4:1110689/149‑1 (MQ=255)
                                                             ttGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCt                                                                                       <  1:378979/149‑1 (MQ=255)
                                                                tCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGAt                                                                                    <  3:33850/149‑1 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                                  >  2:1009313/1‑149 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                                  <  2:857872/149‑1 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttga                                                                                                                                          >  1:123683/1‑91 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                           >  1:206986/1‑91 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                           <  2:206986/92‑2 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAAttt                                                                                                                        <  3:124759/110‑1 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAac                                                                                 >  1:472127/1‑149 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAac                                                                                 >  3:1118512/1‑149 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAac                                                                                 >  2:510322/1‑149 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCGCAACCAAACTTATCTGATAac                                                                                 >  4:1137343/1‑149 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttgat                                                                                                                                         >  3:913086/1‑90 (MQ=255)
                                                                   ccAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttgat                                                                                                                                         <  4:913086/93‑4 (MQ=255)
                                                                    cAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGTGGTTAgc                                                                                                                                                  <  2:1269217/83‑1 (MQ=255)
                                                                    cAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAaca                                                                                >  3:327607/1‑149 (MQ=255)
                                                                      aaGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACt                                                                              >  3:1304108/1‑149 (MQ=255)
                                                                        gTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCACATTCTAAAAATCTCAACCAAACTTATCTGATAACACTaa                                                                            <  2:1360781/149‑1 (MQ=255)
                                                                         ttttCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTaaa                                                                           >  3:287430/1‑149 (MQ=255)
                                                                              gATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGa                                                                      >  2:467642/1‑149 (MQ=255)
                                                                                tGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaaa                                                                    <  2:1022520/149‑1 (MQ=255)
                                                                                tGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaaa                                                                    <  4:789207/149‑1 (MQ=255)
                                                                                   cAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGaa                                                                 <  1:1500736/149‑1 (MQ=255)
                                                                                   cAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGaa                                                                 <  2:1229044/149‑1 (MQ=255)
                                                                                   cAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGaa                                                                 <  4:1000807/149‑1 (MQ=255)
                                                                                   cAATGAGCCAATTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGaa                                                                 <  3:1510270/149‑1 (MQ=255)
                                                                                    aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCaaa                                                                                                                  <  1:1122302/99‑1 (MQ=255)
                                                                                    aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCaaa                                                                                                                  >  2:1122302/1‑99 (MQ=255)
                                                                                    aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAAt                                                                >  1:229257/1‑149 (MQ=255)
                                                                                    aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAAt                                                                >  3:1005951/1‑149 (MQ=255)
                                                                                      tGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGc                                                              <  4:1156624/149‑1 (MQ=255)
                                                                                       gAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCaa                                                                                                   <  1:1398829/111‑1 (MQ=255)
                                                                                       gAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCaa                                                                                                   >  2:1398829/1‑111 (MQ=255)
                                                                                         gCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGccc                                                                                                                                              >  2:89872/1‑66 (MQ=255)
                                                                                         gCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGccc                                                                                                                                              <  1:89872/66‑1 (MQ=255)
                                                                                         gCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTa                                                           <  4:1118512/149‑1 (MQ=255)
                                                                                         gCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTa                                                           >  3:1106551/1‑149 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACa                                                         <  2:118533/149‑1 (MQ=255)
                                                                                            aCTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAg                                                        <  2:357139/149‑1 (MQ=255)
                                                                                             cTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATGTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGc                                                              <  3:1025392/142‑1 (MQ=255)
                                                                                              ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                      <  1:10653/149‑1 (MQ=255)
                                                                                              ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                      <  2:186486/149‑1 (MQ=255)
                                                                                              ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                      >  4:379462/1‑149 (MQ=255)
                                                                                                aGTCAAATCAATCAGGCAAAACCCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAATCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaa                                                    <  3:961409/149‑1 (MQ=255)
                                                                                                 gTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaag                                                   >  2:1311266/1‑149 (MQ=255)
                                                                                                 gTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTAATAACACTAAATTCGAAAGAATGCGTACAGgtaag                                                   >  2:283276/1‑149 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCaaa                                                                                              <  1:66588/105‑1 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCaaa                                                                                              >  2:66588/1‑105 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                                  >  3:501440/1‑149 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                                  <  4:298447/149‑1 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGCGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                                  >  1:518387/1‑149 (MQ=255)
                                                                                                    aaaTCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagtaa                                                <  4:220220/149‑1 (MQ=255)
                                                                                                    aaaTCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagtaa                                                >  4:578930/1‑149 (MQ=255)
                                                                                                     aatcaatcAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAAc                                               >  4:591005/1‑149 (MQ=255)
                                                                                                     aatcaatcAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAAc                                               >  4:434788/1‑149 (MQ=255)
                                                                                                     aatcaatcAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAAc                                               <  1:1432037/149‑1 (MQ=255)
                                                                                                           tcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGaa                                         >  2:763739/1‑149 (MQ=255)
                                                                                                                cAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGaaaaaaa                                    <  2:976305/149‑1 (MQ=255)
                                                                                                                  aaaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTATGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAAtt                                  <  1:764635/149‑1 (MQ=255)
                                                                                                                   aaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTg                                 >  4:1563093/1‑149 (MQ=255)
                                                                                                                     cTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCt                               >  2:134833/1‑149 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCg                                                             <  3:1567296/118‑1 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTa                              >  3:1436280/1‑149 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTa                              >  2:269521/1‑149 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACCTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTa                              <  3:669128/149‑1 (MQ=255)
                                                                                                                        gCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTAtt                            <  1:510322/149‑1 (MQ=255)
                                                                                                                          cTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATGGCTAtttt                          >  3:617172/1‑147 (MQ=255)
                                                                                                                            gACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTgg                        <  3:1140563/149‑1 (MQ=255)
                                                                                                                                gAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCt                                                                                       <  4:151935/82‑1 (MQ=255)
                                                                                                                                  aTTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCg                  >  1:326224/1‑149 (MQ=255)
                                                                                                                                      aaTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGcc              >  1:1328587/1‑149 (MQ=255)
                                                                                                                                            gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACggg        <  4:521505/149‑1 (MQ=255)
                                                                                                                                              gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa      <  1:32723/149‑1 (MQ=255)
                                                                                                                                               cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAACCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt     <  4:1280527/149‑1 (MQ=255)
                                                                                                                                               cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt     <  2:229257/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTA  >  NC_000913/1746880‑1747174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: