Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,770,205 T→A 5.5% intergenic (+19/‑167) ydiK → / → rprA UPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,770,2050TA 5.5% 512.2 / 15.0 146intergenic (+19/‑167)ydiK/rprAUPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (71/67);  new base A (2/6);  total (73/73)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

Only 100 of 151 total aligned reads displayed.

GTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770062‑1770352
                                                                                                                                               |                                                                                                                                                   
gTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGTTGGAAGAAGTCCCGACGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgc                                                                                                                                                >  2:1216574/1‑149 (MQ=255)
 tCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgcc                                                                                                                                               >  1:1303097/1‑149 (MQ=255)
    ggTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGa                                                                                                                                            <  3:1283490/149‑1 (MQ=255)
     gTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAt                                                                                                                                           <  2:476371/149‑1 (MQ=255)
      ttCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGAtt                                                                                                                                          <  2:640159/149‑1 (MQ=255)
          gTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTa                                                                                                                                      >  1:1084008/1‑149 (MQ=255)
          gTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTa                                                                                                                                      <  1:1128628/149‑1 (MQ=255)
           ttAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     >  2:686009/1‑149 (MQ=255)
           ttAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     <  2:819722/149‑1 (MQ=255)
            tAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAac                                                                                                                                    <  2:97169/149‑1 (MQ=255)
            tAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAac                                                                                                                                    <  3:216166/149‑1 (MQ=255)
             aGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAtgaga                                                                                                                                       >  3:1365059/1‑141 (MQ=255)
             aGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAtgaga                                                                                                                                       <  4:1365059/145‑5 (MQ=255)
               ccGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAgccac                                                                                                                                 >  4:1368938/1‑149 (MQ=255)
               ccGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccac                                                                                                                                 >  1:1286870/1‑149 (MQ=255)
                 gTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCg                                                                                                                               >  2:204203/1‑149 (MQ=255)
                  tttCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGc                                                                                                                              <  2:1084008/149‑1 (MQ=255)
                      cTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCTCCTGATTGTTAACCACCGCTAAt                                                                                                                          >  2:875500/1‑149 (MQ=255)
                       tGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         >  1:306892/1‑149 (MQ=255)
                       tGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         >  2:140237/1‑149 (MQ=255)
                       tGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         >  4:1461887/1‑149 (MQ=255)
                       tGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAACTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         <  4:857328/149‑1 (MQ=255)
                         gCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGa                                                                                                                       <  4:697045/149‑1 (MQ=255)
                         gCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGa                                                                                                                       >  2:1466742/1‑149 (MQ=255)
                           gTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGata                                                                                                                     >  1:1095586/1‑149 (MQ=255)
                            tCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    >  3:663767/1‑149 (MQ=255)
                             cTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATAtt                                                                                                                   <  2:740935/149‑1 (MQ=255)
                              tGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATAttt                                                                                                                  <  3:438105/149‑1 (MQ=255)
                                tttGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc                                                                                                                >  4:1276854/1‑149 (MQ=255)
                                 ttGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgct                                                                                                               <  3:1389115/149‑1 (MQ=255)
                                 ttGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgct                                                                                                               <  1:1144364/149‑1 (MQ=255)
                                  tGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgctt                                                                                                              <  1:459675/149‑1 (MQ=255)
                                     cgcgTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTtgcttgct                                                                                                           <  3:51062/149‑1 (MQ=255)
                                      gcgTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc                                                                                                          <  4:663767/149‑1 (MQ=255)
                                      gcgTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc                                                                                                          >  2:752136/1‑149 (MQ=255)
                                            gtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcccc                                                                                                    <  1:752136/149‑1 (MQ=255)
                                              ggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCat                                                                                                  >  1:575427/1‑149 (MQ=255)
                                               gaagaaGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc                                                                                                          >  3:702581/1‑140 (MQ=255)
                                               gaagaaGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc                                                                                                          <  4:702581/140‑1 (MQ=255)
                                               gaagaaGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCata                                                                                                 >  1:1331500/1‑149 (MQ=255)
                                                aagaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                                                                                                >  4:339180/1‑149 (MQ=255)
                                                        ccgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGc                                                                                        >  3:40618/1‑149 (MQ=255)
                                                        ccgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGc                                                                                        >  2:915816/1‑149 (MQ=255)
                                                                gACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                                                                                     <  3:1266990/128‑1 (MQ=255)
                                                                gACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                                                                                     >  4:1266990/1‑128 (MQ=255)
                                                                gACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGcc                                                                                >  4:417930/1‑149 (MQ=255)
                                                                   ggACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAtt                                                                             <  2:1341318/149‑1 (MQ=255)
                                                                       cAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGc                                                                         >  3:519150/1‑149 (MQ=255)
                                                                        aaCCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCt                                                                        <  1:1552133/149‑1 (MQ=255)
                                                                         aCCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     >  4:1002437/1‑87 (MQ=255)
                                                                         aCCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     <  3:1002437/87‑1 (MQ=255)
                                                                          ccGGAAGAAATTCTAGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg                                                                      >  1:1195208/1‑149 (MQ=255)
                                                                           cGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt                                                                     <  3:1412250/149‑1 (MQ=255)
                                                                            ggAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgta                                                                    <  3:762422/149‑1 (MQ=255)
                                                                            ggAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgta                                                                    >  3:909801/1‑149 (MQ=255)
                                                                                gaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAc                                                                <  2:1095586/149‑1 (MQ=255)
                                                                                 aaaTTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAcc                                                               >  3:934257/1‑149 (MQ=255)
                                                                                  aaTTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc                                                                                                      >  1:294129/1‑109 (MQ=255)
                                                                                  aaTTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCt                                                              >  2:1595123/1‑149 (MQ=255)
                                                                                     tctcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTc                                                           <  1:1352893/149‑1 (MQ=255)
                                                                                     tctcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTc                                                           <  4:683170/149‑1 (MQ=255)
                                                                                       tcGAAGAACTTGGCGAAATCGACAAACCGAATAAGTAATTTCTCATCGGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCa                                                                               <  3:999876/127‑1 (MQ=255)
                                                                                         gaagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCaa                                                       >  2:1099829/1‑149 (MQ=255)
                                                                                          aagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCaaa                                                      >  2:569647/1‑149 (MQ=255)
                                                                                          aagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCaaa                                                      >  2:1340656/1‑149 (MQ=255)
                                                                                              aCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATAttt                                                                                                                  >  2:1304551/1‑85 (MQ=255)
                                                                                               cTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCtt                                                 <  4:239490/149‑1 (MQ=255)
                                                                                                ttGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCttt                                                <  1:1466742/149‑1 (MQ=255)
                                                                                                 tGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTa                                               <  4:519150/149‑1 (MQ=255)
                                                                                                     gAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGt                                           <  3:681170/149‑1 (MQ=255)
                                                                                                      aaaTCGAGAAACAGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGACTGATTGTTAACCACCGCTAATTGATATTTGTTTGCTCTTCCCCATATTTCCCTGCTTACCGCCCTTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGtt                                          <  4:467321/149‑1 (MQ=255)
                                                                                                       aaTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    <  2:411768/74‑1 (MQ=255)
                                                                                                       aaTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    >  1:411768/1‑74 (MQ=255)
                                                                                                        aTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGtttt                                        >  1:992038/1‑149 (MQ=255)
                                                                                                           gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaa                                     >  2:1316358/1‑149 (MQ=255)
                                                                                                           gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaa                                     >  2:1029848/1‑149 (MQ=255)
                                                                                                            agaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    <  1:1029848/149‑1 (MQ=255)
                                                                                                            agaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    <  3:728830/149‑1 (MQ=255)
                                                                                                            agaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    <  1:1316358/149‑1 (MQ=255)
                                                                                                            agaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    >  3:237164/1‑149 (MQ=255)
                                                                                                                aCCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTaa                                <  2:575427/149‑1 (MQ=255)
                                                                                                                   gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATga                             >  4:1534702/1‑149 (MQ=255)
                                                                                                                   gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATga                             <  4:237164/149‑1 (MQ=255)
                                                                                                                   gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATga                             <  3:1465465/149‑1 (MQ=255)
                                                                                                                   gAATAAGTAATATCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAAGTCTTTACAGTTTTTAAACTAATga                             >  3:1034252/1‑149 (MQ=255)
                                                                                                                    aaTAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATgag                            >  3:151541/1‑149 (MQ=255)
                                                                                                                      tAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGAc                          <  1:601614/149‑1 (MQ=255)
                                                                                                                         gTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGaa                       >  4:1178389/1‑149 (MQ=255)
                                                                                                                          tAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAAt                      >  3:1333683/1‑149 (MQ=255)
                                                                                                                            aTTTCTCATCAGGCGGCTCTGCCTCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCt                    <  1:875500/149‑1 (MQ=255)
                                                                                                                                  aatcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATc                <  3:44811/146‑1 (MQ=255)
                                                                                                                                   atcaGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGAc             <  1:1385780/149‑1 (MQ=255)
                                                                                                                                      aGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCa          >  4:1180046/1‑149 (MQ=255)
                                                                                                                                      aGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCa          >  1:1552618/1‑149 (MQ=255)
                                                                                                                                           gcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAg     >  1:1407486/1‑149 (MQ=255)
                                                                                                                                            ctctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGt    <  2:1286870/149‑1 (MQ=255)
                                                                                                                                            ctctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGt    <  1:686009/149‑1 (MQ=255)
                                                                                                                                             tctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTc   >  3:779081/1‑149 (MQ=255)
                                                                                                                                              ctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  3:339180/149‑1 (MQ=255)
                                                                                                                                              ctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  3:1217313/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770062‑1770352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: