Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,680 T→A 7.1% intergenic (+24/+17) yniA → / ← yniB fructosamine kinase family protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,6800TA 7.1% 672.8 / 31.0 196intergenic (+24/+17)yniA/yniBfructosamine kinase family protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (92/90);  new base A (9/5);  total (101/95)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 198 total aligned reads displayed.

CTGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808532‑1808828
                                                                                                                                                    |                                                                                                                                                    
    cGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGt                                                                                                                                                  >  4:691089/1‑149 (MQ=255)
    cGATTTCCTTGAACGTCAACCGGTTTAACAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGt                                                                                                                                                  >  3:1050616/1‑149 (MQ=255)
     gATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTg                                                                                                                                                 >  4:646659/1‑149 (MQ=255)
         tCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGc                                                                                                                                             <  2:1126621/149‑1 (MQ=255)
          ccTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGcc                                                                                                                                            >  3:368826/1‑149 (MQ=255)
           cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCt                                                                                                                                           >  1:395394/1‑149 (MQ=255)
           cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCt                                                                                                                                           >  4:1237896/1‑149 (MQ=255)
            ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                          >  4:1430746/1‑149 (MQ=255)
            ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                          <  2:1562019/149‑1 (MQ=255)
            ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                          >  1:494979/1‑149 (MQ=255)
             tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttt                                                                                                                                         <  1:1325367/149‑1 (MQ=255)
              gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                        <  3:1561277/149‑1 (MQ=255)
              gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                        <  1:637444/149‑1 (MQ=255)
              gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCatta                                                                                                                                        <  4:767916/149‑2 (MQ=255)
                  gTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAtt                                                                                                                                    >  1:778903/1‑149 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCTGCCTTTTGATtaaa                                                                                                                                 <  3:112335/149‑1 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  4:267871/1‑149 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  1:873069/1‑149 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  1:891008/1‑149 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGcca                                                                                                                                           <  2:617850/139‑2 (MQ=255)
                      aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                >  2:200379/1‑149 (MQ=255)
                      aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                >  4:50813/1‑149 (MQ=255)
                      aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                >  1:961372/1‑149 (MQ=255)
                      aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                <  1:1271037/149‑1 (MQ=255)
                         ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataaa                                                                                                                             >  2:1300475/1‑149 (MQ=255)
                         ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataaa                                                                                                                             >  4:444958/1‑149 (MQ=255)
                               ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAg                                                                                                                       >  3:997659/1‑149 (MQ=255)
                                cAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                       <  3:1035183/132‑1 (MQ=255)
                                cAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                      >  3:814094/1‑149 (MQ=255)
                                cAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                      >  3:944009/1‑149 (MQ=255)
                                 aaCTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCa                                                                                                                     >  2:1519555/1‑149 (MQ=255)
                                       acacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaa                                                                                                               <  3:546295/149‑1 (MQ=255)
                                       acacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaa                                                                                                               >  3:382607/1‑149 (MQ=255)
                                        cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                              <  4:924161/149‑1 (MQ=255)
                                        cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                              <  3:267871/149‑1 (MQ=255)
                                         acGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAg                                                                                                             >  3:1258536/1‑149 (MQ=255)
                                             tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                         >  1:771814/1‑149 (MQ=255)
                                              gTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAacac                                                                                                        >  3:603932/1‑149 (MQ=255)
                                               ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  2:431160/1‑149 (MQ=255)
                                                  aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATaa                                                                                                    <  4:382607/149‑1 (MQ=255)
                                                  aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATaa                                                                                                    <  2:762390/149‑1 (MQ=255)
                                                    tCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCATCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAAcc                                                                                                  <  1:1300475/149‑1 (MQ=255)
                                                       tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                               <  2:923309/149‑1 (MQ=255)
                                                       tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                               <  1:1514599/149‑1 (MQ=255)
                                                        gCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgca                                                                                              >  1:1053014/1‑149 (MQ=255)
                                                              ttATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTCGATAGATTATTAGCAGCATGATATGGGCTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGTAGAGAAAAGAACACATAATCTGCAGCAATa                                                                                        >  4:1312407/1‑149 (MQ=255)
                                                                  ttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                    <  2:395394/149‑1 (MQ=255)
                                                                     gCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATaa                                                                                 <  2:494979/149‑1 (MQ=255)
                                                                        gTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACGAATAACCg                                                                              >  3:30618/1‑149 (MQ=255)
                                                                         tCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCaga                                                                                                                   >  1:204215/1‑111 (MQ=255)
                                                                          cAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGc                                                                            >  4:1097566/1‑149 (MQ=255)
                                                                            gCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                          <  3:646659/149‑1 (MQ=255)
                                                                             cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                         <  1:351660/149‑1 (MQ=255)
                                                                              agttGGTTATTTCTAAGAAGTGATTGGAGAGACTAGTAGTAGAATTATATGGGTTGAGGATAATGGGCGCTCTGTGCGGCCTTTTGAATAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAAACGGCAggg                                                                        <  4:17306/147‑2 (MQ=255)
                                                                               tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGccg                                                                          <  1:744932/146‑3 (MQ=255)
                                                                               tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                          >  2:744932/1‑146 (MQ=255)
                                                                                    ttATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACa                                                                  <  3:40851/149‑1 (MQ=255)
                                                                                     tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                 <  1:1412700/149‑1 (MQ=255)
                                                                                      aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACGAATAACCGGCAGGATATACAGt                                                                <  4:30618/149‑1 (MQ=255)
                                                                                             agcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaaa                                                         >  4:1527913/1‑149 (MQ=255)
                                                                                              gcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaaaa                                                        <  4:1050616/149‑1 (MQ=255)
                                                                                                agTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACt                                                      >  1:632921/1‑149 (MQ=255)
                                                                                                  tCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGc                                                    >  3:139821/1‑149 (MQ=255)
                                                                                                   cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAGATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   <  2:109780/149‑1 (MQ=255)
                                                                                                   cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                   <  3:877036/149‑1 (MQ=255)
                                                                                                    aTTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATAc                                                                                       <  3:763157/112‑1 (MQ=255)
                                                                                                       ggATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaa                                               >  2:223143/1‑149 (MQ=255)
                                                                                                        gATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaa                                              >  4:1282719/1‑149 (MQ=255)
                                                                                                         aTAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaaa                                             <  2:891008/149‑1 (MQ=255)
                                                                                                           aGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                           <  3:50813/149‑1 (MQ=255)
                                                                                                                  taGCAGCATGATATCGGTTGAGGATAATGGCTGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACGTGCAGCAATACAAATAACTGGCCGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGt                                    <  3:1312407/149‑1 (MQ=255)
                                                                                                                    gcagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATg                                      <  4:1172885/145‑1 (MQ=255)
                                                                                                                    gcagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATg                                      >  3:1172885/1‑145 (MQ=255)
                                                                                                                      agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                           >  1:731101/1‑106 (MQ=255)
                                                                                                                      agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                           <  2:731101/106‑1 (MQ=255)
                                                                                                                      agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCgg                                >  3:102439/1‑149 (MQ=255)
                                                                                                                       gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGa                               >  1:1312379/1‑149 (MQ=255)
                                                                                                                        caTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                              <  2:604255/149‑1 (MQ=255)
                                                                                                                                  gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGCAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                          >  4:412802/1‑95 (MQ=255)
                                                                                                                                  gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                          <  3:412802/95‑1 (MQ=255)
                                                                                                                                  gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACg                    <  4:603932/149‑1 (MQ=255)
                                                                                                                                  gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACg                    <  3:70428/149‑1 (MQ=255)
                                                                                                                                   ttGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                   <  3:1485362/149‑1 (MQ=255)
                                                                                                                                   ttGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                   <  3:166541/149‑1 (MQ=255)
                                                                                                                                   ttGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                   <  4:368826/149‑1 (MQ=255)
                                                                                                                                     gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGa                 >  2:1382706/1‑149 (MQ=255)
                                                                                                                                        tcaaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGAAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGCCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTc              >  1:1132867/7‑149 (MQ=255)
                                                                                                                                        gATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAAc                                                                                <  2:1254655/83‑1 (MQ=255)
                                                                                                                                          tAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCaa            <  4:889779/149‑1 (MQ=255)
                                                                                                                                          aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                           >  4:593039/5‑118 (MQ=255)
                                                                                                                                          aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                           <  3:593039/114‑1 (MQ=255)
                                                                                                                                          aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCaa            >  3:244840/5‑149 (MQ=255)
                                                                                                                                           aaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAt           >  1:1081734/4‑149 (MQ=255)
                                                                                                                                            aTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAAt                                                                                   <  3:44974/76‑1 (MQ=255)
                                                                                                                                             aggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt         >  1:1239014/2‑149 (MQ=255)
                                                                                                                                              ggCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTg        <  4:160466/149‑1 (MQ=255)
                                                                                                                                                ccGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGtt      <  4:617758/149‑1 (MQ=255)
                                                                                                                                                 cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc     <  2:778903/149‑1 (MQ=255)
                                                                                                                                                 cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc     <  4:947268/149‑1 (MQ=255)
                                                                                                                                                  gCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaa                           <  1:1213171/126‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808532‑1808828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: