Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,684 T→A 7.1% intergenic (+28/+13) yniA → / ← yniB fructosamine kinase family protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,6840TA 7.1% 671.2 / 27.4 195intergenic (+28/+13)yniA/yniBfructosamine kinase family protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (89/92);  new base A (9/5);  total (98/97)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

CGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTT  >  NC_000913/1808536‑1808832
                                                                                                                                                    |                                                                                                                                                    
cGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGt                                                                                                                                                      >  4:691089/1‑149 (MQ=255)
cGATTTCCTTGAACGTCAACCGGTTTAACAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGt                                                                                                                                                      >  3:1050616/1‑149 (MQ=255)
 gATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTg                                                                                                                                                     >  4:646659/1‑149 (MQ=255)
       cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCt                                                                                                                                               >  1:395394/1‑149 (MQ=255)
        ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGGTAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                              >  3:1537124/1‑149 (MQ=255)
        ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                              <  2:1562019/149‑1 (MQ=255)
        ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                              >  1:494979/1‑149 (MQ=255)
         tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttt                                                                                                                                             <  1:1325367/149‑1 (MQ=255)
          gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                            <  2:1116317/149‑1 (MQ=255)
          gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                            <  1:637444/149‑1 (MQ=255)
          gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttct                                                                                                                                            <  4:1077521/149‑3 (MQ=255)
          gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCatta                                                                                                                                            <  4:767916/149‑2 (MQ=255)
              gTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAtt                                                                                                                                        >  1:778903/1‑149 (MQ=255)
               tCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATta                                                                                                                                       >  3:609981/1‑149 (MQ=255)
                cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccattatc                                                                                                                                          >  3:241325/1‑139 (MQ=255)
                 aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                     <  3:567400/149‑1 (MQ=255)
                 aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGcca                                                                                                                                               <  2:617850/139‑2 (MQ=255)
                  aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                    <  1:1271037/149‑1 (MQ=255)
                  aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                    >  2:200379/1‑149 (MQ=255)
                  aCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                    >  4:50813/1‑149 (MQ=255)
                     ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataaa                                                                                                                                 >  2:1300475/1‑149 (MQ=255)
                     ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataaa                                                                                                                                 >  4:444958/1‑149 (MQ=255)
                      gTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                                <  3:26301/149‑1 (MQ=255)
                           ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAg                                                                                                                           >  3:160466/1‑149 (MQ=255)
                            cAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                           >  4:1035183/1‑132 (MQ=255)
                            cAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                           <  3:1035183/132‑1 (MQ=255)
                             aaCTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                                >  1:893910/1‑142 (MQ=255)
                             aaCTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCa                                                                                                                         >  2:1519555/1‑149 (MQ=255)
                               ctctACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCaga                                                                                                                       <  4:195672/149‑1 (MQ=255)
                                  tACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaa                                                                                                                    >  3:1294766/1‑149 (MQ=255)
                                    cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                                  <  4:924161/149‑1 (MQ=255)
                                     acGCTGTTAAATTGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                          >  3:115803/1‑124 (MQ=255)
                                     acGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                          <  4:115803/124‑1 (MQ=255)
                                     acGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAtt                                                                                                                                        >  3:537110/1‑126 (MQ=255)
                                       gCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                               <  3:309693/149‑1 (MQ=255)
                                       gCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                               <  4:1537124/149‑1 (MQ=255)
                                         tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                             <  3:412240/149‑1 (MQ=255)
                                         tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                             >  1:771814/1‑149 (MQ=255)
                                           ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                           >  3:1479518/1‑149 (MQ=255)
                                           ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                           >  2:431160/1‑149 (MQ=255)
                                              aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTCTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATaa                                                                                                        >  4:1485362/1‑149 (MQ=255)
                                              aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATaa                                                                                                        <  2:762390/149‑1 (MQ=255)
                                              aaTCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATaa                                                                                                        <  4:31333/149‑1 (MQ=255)
                                                tCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCATCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAAcc                                                                                                      <  1:1300475/149‑1 (MQ=255)
                                                tCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAAcc                                                                                                      <  3:444958/149‑1 (MQ=255)
                                                   tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                                   <  2:923309/149‑1 (MQ=255)
                                                   tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                                   <  1:1514599/149‑1 (MQ=255)
                                                    gCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgca                                                                                                  >  1:1053014/1‑149 (MQ=255)
                                                      aaGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgcagc                                                                                                >  4:27681/1‑149 (MQ=255)
                                                          ttATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATa                                                                                            >  1:865854/1‑149 (MQ=255)
                                                              ttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                        <  2:395394/149‑1 (MQ=255)
                                                              ttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                        <  3:27681/149‑1 (MQ=255)
                                                              ttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                        >  3:467683/1‑149 (MQ=255)
                                                                 gCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATaa                                                                                     <  2:494979/149‑1 (MQ=255)
                                                                     tCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCaga                                                                                                                       >  1:204215/1‑111 (MQ=255)
                                                                         cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                             <  1:351660/149‑1 (MQ=255)
                                                                          aTTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGa                                                                            <  4:1374398/149‑1 (MQ=255)
                                                                           tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGccg                                                                              <  1:744932/146‑3 (MQ=255)
                                                                           tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                              >  2:744932/1‑146 (MQ=255)
                                                                                 tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                     <  1:1412700/149‑1 (MQ=255)
                                                                                 tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                     <  4:1258536/149‑1 (MQ=255)
                                                                                  aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGt                                                                    >  3:656366/1‑149 (MQ=255)
                                                                                         agcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaaa                                                             >  4:166541/1‑149 (MQ=255)
                                                                                            agTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACt                                                          >  1:632921/1‑149 (MQ=255)
                                                                                               cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAAGACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCa                                                       <  4:1294766/149‑1 (MQ=255)
                                                                                                 ttGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAgg                                                     >  2:1036057/1‑149 (MQ=255)
                                                                                                   ggATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaa                                                   >  2:223143/1‑149 (MQ=255)
                                                                                                    gATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaa                                                  >  3:889779/1‑149 (MQ=255)
                                                                                                     aTAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaaa                                                 <  2:891008/149‑1 (MQ=255)
                                                                                                           tattaGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTAt                                           >  4:1212228/1‑149 (MQ=255)
                                                                                                              taGCAGCATGATATCGGTTGAGGATAATGGCTGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACGTGCAGCAATACAAATAACTGGCCGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGt                                        <  3:1312407/149‑1 (MQ=255)
                                                                                                                gcagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                              >  3:603641/1‑109 (MQ=255)
                                                                                                                gcagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGc                                      >  3:790776/1‑149 (MQ=255)
                                                                                                                  agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                               <  2:731101/106‑1 (MQ=255)
                                                                                                                  agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                               >  1:731101/1‑106 (MQ=255)
                                                                                                                  agcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCgg                                    >  3:102439/1‑149 (MQ=255)
                                                                                                                   gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGa                                   >  1:1312379/1‑149 (MQ=255)
                                                                                                                    caTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                                  <  2:604255/149‑1 (MQ=255)
                                                                                                                    caTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                                  <  3:1097566/149‑1 (MQ=255)
                                                                                                                    caTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                                  <  3:1212228/149‑1 (MQ=255)
                                                                                                                     aTGATATGGGTTGAGGATAATGGCCGCTCCGTGGGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaac                                 >  3:992568/1‑149 (MQ=255)
                                                                                                                       gATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaa                               <  3:70763/149‑1 (MQ=255)
                                                                                                                           tGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAAt                           >  3:819243/1‑149 (MQ=255)
                                                                                                                              gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGCAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAg                                                                              >  4:412802/1‑95 (MQ=255)
                                                                                                                              gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACg                        <  4:603932/149‑1 (MQ=255)
                                                                                                                                 gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGa                     >  2:1382706/1‑149 (MQ=255)
                                                                                                                                    tcaaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGAAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGCCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTc                  >  1:1132867/7‑149 (MQ=255)
                                                                                                                                      tAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCaa                <  4:889779/149‑1 (MQ=255)
                                                                                                                                      aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATa                                               >  4:593039/5‑118 (MQ=255)
                                                                                                                                       aaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAt               >  1:1081734/4‑149 (MQ=255)
                                                                                                                                        aaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAtt              >  3:400079/3‑149 (MQ=255)
                                                                                                                                         aggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt             >  1:1239014/2‑149 (MQ=255)
                                                                                                                                           gCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGata                                                                          >  4:378219/1‑86 (MQ=255)
                                                                                                                                           gCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGata                                                                          <  3:378219/86‑1 (MQ=255)
                                                                                                                                            ccGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGtt          <  4:617758/149‑1 (MQ=255)
                                                                                                                                             cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc         <  2:778903/149‑1 (MQ=255)
                                                                                                                                             cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTc         <  4:947268/149‑1 (MQ=255)
                                                                                                                                              gCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaa                               >  2:1213171/1‑126 (MQ=255)
                                                                                                                                              gCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaa                               <  1:1213171/126‑1 (MQ=255)
                                                                                                                                                tCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCAcg      <  1:890730/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTT  >  NC_000913/1808536‑1808832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: