Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 191,739 | T→C | 6.3% | intergenic (+31/‑116) | tsf → / → pyrH | translation elongation factor EF‑Ts/uridylate kinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 191,739 | 0 | T | C | 6.3% | 612.4 / 22.2 | 190 | intergenic (+31/‑116) | tsf/pyrH | translation elongation factor EF‑Ts/uridylate kinase |
Reads supporting (aligned to +/- strand): ref base T (96/82); new base C (6/6); total (102/88) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-02 |
Only 100 of 197 total aligned reads displayed.
TGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAAC > NC_000913/191593‑191885 | gAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGcttctt < 1:691686/149‑1 (MQ=255) gAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGcttctt > 3:345414/1‑149 (MQ=255) aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttt < 4:675750/149‑1 (MQ=255) aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttt > 1:594363/1‑149 (MQ=255) aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttt < 1:387256/149‑1 (MQ=255) aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttt > 3:167992/1‑149 (MQ=255) agagCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttttt < 2:366805/149‑1 (MQ=255) agagCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttttt < 2:1446049/149‑1 (MQ=255) agagCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCttttt < 2:1100368/149‑1 (MQ=255) agagCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGAAGCCG‑CCCTCAGGCGGCTCCttttt < 2:1358700/149‑1 (MQ=255) cATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGc < 3:193565/149‑1 (MQ=255) cATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGAAGCCG‑CCCTCAGGCGGCTCCTTTTtgata < 3:1332906/149‑4 (MQ=38) aTAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGcc > 3:246191/1‑149 (MQ=255) aTAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGcc > 2:1044596/1‑149 (MQ=255) cGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGcttct < 3:874578/136‑1 (MQ=255) gAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTTTTTTTGTGCCCATCTTGt < 1:1053642/149‑1 (MQ=255) aaGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTa > 1:760658/1‑149 (MQ=255) gACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAAtt > 1:1028214/1‑149 (MQ=255) aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTc < 3:291749/149‑1 (MQ=255) aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTc > 2:100671/1‑149 (MQ=255) tGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCTATCTTGTAAATTCAg < 2:1327540/149‑1 (MQ=255) ggCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGc > 2:698938/1‑149 (MQ=255) ggCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGc > 2:983545/1‑149 (MQ=255) ggCTTCATCCGCGTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGc < 2:1236677/149‑1 (MQ=255) tCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAAc > 4:846145/1‑149 (MQ=255) tCATCCGCTTCGAAGGGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCAAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAAc > 4:171339/1‑149 (MQ=255) gCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgt < 4:688311/149‑1 (MQ=255) gCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgt < 3:967625/149‑1 (MQ=255) gCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgt < 2:707948/149‑1 (MQ=255) cTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgtg > 1:1084875/1‑149 (MQ=255) gAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGc > 4:536172/1‑149 (MQ=255) ggTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCt > 4:390411/1‑149 (MQ=255) gTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTg > 4:337642/1‑149 (MQ=255) tGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGa > 4:1223260/1‑149 (MQ=255) aGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGaaaa < 1:36706/149‑1 (MQ=255) aGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGaaaa > 1:281263/1‑149 (MQ=255) gCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGc < 4:1350054/149‑1 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc < 2:726535/149‑1 (MQ=255) tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc > 3:368815/1‑149 (MQ=255) gagaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGt > 3:337171/1‑149 (MQ=255) gagaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGt > 4:498099/1‑149 (MQ=255) agaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTa > 3:271756/1‑149 (MQ=255) gTTGAGACTGACTTTGCAGCAGCAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATg > 4:1232387/1‑149 (MQ=255) gTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATg > 3:842931/1‑149 (MQ=255) ttGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGt > 4:190865/1‑149 (MQ=255) ttGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGt < 2:762647/149‑1 (MQ=255) tGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTc < 4:1286404/149‑1 (MQ=255) agaCTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGc < 1:1369492/149‑1 (MQ=255) actgactTTGCAGCAGAAGTTGCTGCGATGTCCGAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTa < 1:872118/149‑1 (MQ=255) ctgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAg > 3:920930/1‑149 (MQ=255) tgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACaa > 3:589695/1‑139 (MQ=255) tgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACaa < 4:589695/139‑1 (MQ=255) ctTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGGGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTAtt < 4:604123/149‑1 (MQ=255) ttGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTaa < 4:225235/149‑1 (MQ=255) gTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAAt > 3:992285/1‑149 (MQ=255) ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt < 3:158635/149‑1 (MQ=255) ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt < 2:1084875/149‑1 (MQ=255) ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt < 2:803537/149‑1 (MQ=255) tgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt < 3:507337/148‑1 (MQ=255) tCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTTAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtct > 4:905063/1‑149 (MQ=255) tCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtct > 3:665259/1‑149 (MQ=255) tCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtct < 1:111953/149‑1 (MQ=255) ccAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTgc < 4:92671/91‑1 (MQ=255) ccAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc < 2:1536046/149‑1 (MQ=255) cAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGtt > 2:47085/1‑139 (MQ=255) cAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCa > 1:837591/1‑149 (MQ=255) cAAGCAGTCCTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCa > 3:496485/1‑149 (MQ=255) aaGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTTGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAg < 4:368815/149‑1 (MQ=255) aaGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc < 2:1207249/147‑1 (MQ=255) aGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaa < 3:1415821/149‑1 (MQ=255) aGTCTTAATTATCAAAAAGGAGCAG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAACAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGCCTCAGgaaa < 4:1423878/149‑1 (MQ=255) cTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaa < 4:351622/149‑1 (MQ=255) ttAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaaa > 1:1198200/1‑149 (MQ=255) aTTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACAt > 2:1150407/1‑149 (MQ=255) aTTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACAt > 3:601680/1‑149 (MQ=255) tATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATgg < 3:1223260/149‑1 (MQ=255) aTCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGc > 1:1554081/1‑149 (MQ=255) cAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTa > 3:224699/1‑149 (MQ=255) cAAAAAGAAGCCGCCCTCA‑GGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc < 1:557934/86‑1 (MQ=21) cAAAAAGAAGCCGCCCTCA‑GGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc > 2:557934/1‑86 (MQ=21) aaaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAc > 4:1494440/1‑149 (MQ=255) aaaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAc > 2:145485/1‑149 (MQ=255) aaaaaGAAGCCGCCCTCA‑GGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGc < 4:279504/82‑1 (MQ=21) aaaaaGAAGCCG‑CCCTCAGGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAc > 4:1312496/1‑149 (MQ=255) aaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTgg > 1:1319969/1‑65 (MQ=255) aaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAAt < 1:1224348/116‑1 (MQ=255) aaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc > 4:310681/1‑149 (MQ=255) aaaaGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAAAGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc > 3:1484947/1‑149 (MQ=255) aaaaGAAGCCG‑CCCTCAGGCGGCTCCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc > 1:649529/1‑149 (MQ=255) aGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAAt < 3:317671/149‑1 (MQ=255) aGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAAt < 1:166314/149‑1 (MQ=255) ccg‑ccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCaaa > 2:1491955/1‑149 (MQ=255) ccg‑ccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCaaa > 1:150794/1‑149 (MQ=255) ccg‑ccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCaaa > 2:1520591/1‑149 (MQ=255) ggcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTGACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGa < 4:109164/102‑1 (MQ=255) ggcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGa > 3:109164/1‑102 (MQ=255) gcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaa < 2:1294846/114‑1 (MQ=255) gcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaa > 1:1294846/1‑114 (MQ=255) gcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATaaa > 1:710538/1‑149 (MQ=255) cttcttTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAAc > 1:1025065/1‑149 (MQ=255) | TGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCG‑CCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAAC > NC_000913/191593‑191885 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |