Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,297 C→A 6.3% intergenic (‑177/+88) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,2970CA 6.3% 500.7 / 14.4 145intergenic (‑177/+88)yfcJ/fabBputative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base C (77/58);  new base A (3/6);  total (81/64)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 146 total aligned reads displayed.

GTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTTA  >  NC_000913/2440153‑2440445
                                                                                                                                                |                                                                                                                                                     
gTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGt                                                                                                                                                  <  1:435432/149‑1 (MQ=255)
    ccccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                              <  2:1490163/149‑1 (MQ=255)
     cccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCt                                                                                                                                             >  2:1467820/1‑149 (MQ=255)
      ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtt                                                                                                                                            >  3:1569228/1‑149 (MQ=255)
        gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttt                                                                                                                                          <  1:1017775/149‑1 (MQ=255)
        gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                          <  4:1565233/149‑1 (MQ=255)
        gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                          <  1:1467820/149‑1 (MQ=255)
         tctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                         >  4:776242/1‑149 (MQ=255)
           tcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCg                                                                                                                                       >  1:670288/1‑149 (MQ=255)
            cATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttttcac                                                                                                                                      <  4:818935/149‑5 (MQ=255)
            cATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGt                                                                                                                                      <  1:239166/149‑1 (MQ=255)
             aTCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                         <  4:1346743/145‑2 (MQ=255)
             aTCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGtt                                                                                                                                     >  3:255418/1‑149 (MQ=255)
             aTCTCTCTGGTTTCAGGGTTACGGTGCGCTGGCAGGATTTAACGCGTACGTCTTTTTAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                         >  3:1346743/1‑144 (MQ=255)
              tctctcTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                    >  4:899966/1‑149 (MQ=255)
               ctctctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGTCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                    <  4:1482642/148‑1 (MQ=255)
                tctctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGt                                                                                                                                  >  1:1133612/1‑149 (MQ=255)
                  tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGc                                                                                                                                >  1:645907/1‑149 (MQ=255)
                           aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCg                                                                                                                       >  2:11180/1‑149 (MQ=255)
                            gggTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGc                                                                                                                      >  3:597418/1‑149 (MQ=255)
                             ggTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCa                                                                                                                     >  1:1590413/1‑149 (MQ=255)
                                tACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCa                                                                                                                     <  2:853306/146‑1 (MQ=255)
                                tACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCAACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCa                                                                                                                     >  1:853306/1‑146 (MQ=255)
                                   ggTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcg                                                                                                               >  3:765407/1‑149 (MQ=255)
                                    gTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcgc                                                                                                              <  2:1099562/149‑1 (MQ=255)
                                        gTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTaa                                                                                                          <  4:519646/149‑1 (MQ=255)
                                            gCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                      <  3:1452728/149‑1 (MQ=255)
                                              aGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCgg                                                                                                    >  2:69597/1‑149 (MQ=255)
                                              aGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCgg                                                                                                    >  1:408611/1‑149 (MQ=255)
                                                gATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGAt                                                                                                  >  1:832680/1‑149 (MQ=255)
                                                  tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                                <  1:69597/149‑1 (MQ=255)
                                                       cgcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGc                                                                                           <  3:170778/149‑1 (MQ=255)
                                                                      aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                            >  3:246813/1‑149 (MQ=255)
                                                                       gAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCg                                                                           >  3:971270/1‑149 (MQ=255)
                                                                          ggAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                        >  1:950709/1‑149 (MQ=255)
                                                                          ggAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                        <  3:943121/149‑1 (MQ=255)
                                                                                   cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGaa                                                               <  3:1436169/149‑1 (MQ=255)
                                                                                   cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGaa                                                               <  2:1459521/149‑1 (MQ=255)
                                                                                   cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGaa                                                               <  1:1102304/149‑1 (MQ=255)
                                                                                    aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                              >  3:1563594/1‑149 (MQ=255)
                                                                                        cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                          <  2:1340823/149‑1 (MQ=255)
                                                                                         gggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATc                                                         <  4:332341/149‑1 (MQ=255)
                                                                                          ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                        <  3:1191151/149‑1 (MQ=255)
                                                                                              gTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                    <  1:1320030/149‑1 (MQ=255)
                                                                                              gTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                    <  2:832680/149‑1 (MQ=255)
                                                                                               tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAg                                                   >  3:812731/1‑149 (MQ=255)
                                                                                               tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAg                                                   >  4:317010/1‑149 (MQ=255)
                                                                                               tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAg                                                   >  4:481069/1‑149 (MQ=255)
                                                                                               tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAg                                                   >  2:141603/1‑149 (MQ=255)
                                                                                                     tGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgcg                                             >  2:1415210/1‑149 (MQ=255)
                                                                                                      ggAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgcgc                                            >  1:303054/1‑149 (MQ=255)
                                                                                                             gcggcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCa                                     <  1:1260353/149‑1 (MQ=255)
                                                                                                             gcggcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCa                                     >  3:1387447/1‑149 (MQ=255)
                                                                                                              cggcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAg                                    <  4:1386055/149‑1 (MQ=255)
                                                                                                                 cgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGt                                 <  3:317010/149‑1 (MQ=255)
                                                                                                                  gATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTg                                <  3:649657/149‑1 (MQ=255)
                                                                                                                     tGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCg                             >  1:1342773/1‑149 (MQ=255)
                                                                                                                      gTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                           >  4:590246/1‑118 (MQ=255)
                                                                                                                      gTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                           <  3:590246/118‑1 (MQ=255)
                                                                                                                       tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                           >  2:443106/1‑149 (MQ=255)
                                                                                                                       tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                           <  1:504673/149‑1 (MQ=255)
                                                                                                                       tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                           >  2:1540397/1‑149 (MQ=255)
                                                                                                                       tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                           >  2:379506/1‑149 (MQ=255)
                                                                                                                        cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                          <  1:1587323/149‑1 (MQ=255)
                                                                                                                        cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                          <  1:1300318/149‑1 (MQ=255)
                                                                                                                         aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATc                                                         <  1:1079173/117‑1 (MQ=255)
                                                                                                                         aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATc                                                         >  2:1079173/1‑117 (MQ=255)
                                                                                                                         aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                         >  3:1306654/1‑149 (MQ=255)
                                                                                                                         aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                         >  3:902252/1‑149 (MQ=255)
                                                                                                                         aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                         <  3:904404/149‑1 (MQ=255)
                                                                                                                          atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                        >  4:709588/1‑149 (MQ=255)
                                                                                                                           tgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGCATCCGACgt                                                                                           >  4:196805/1‑80 (MQ=255)
                                                                                                                                tgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgcc                  >  3:1219948/1‑149 (MQ=255)
                                                                                                                                tgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgcc                  >  1:795101/1‑149 (MQ=255)
                                                                                                                                 gcaaaaGGGAACCTGATGGTTCCCTTTTTCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                           <  3:648819/105‑1 (MQ=38)
                                                                                                                                 gcaaaaGGGAACCTGATGGTTCCCTTTTTCGTGAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                           >  4:648819/3‑107 (MQ=38)
                                                                                                                                 gAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                        <  2:146487/110‑1 (MQ=255)
                                                                                                                                 gAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                        >  1:146487/1‑110 (MQ=255)
                                                                                                                                  aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTGATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga                >  1:970111/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTc                                                     >  4:98896/1‑112 (MQ=255)
                                                                                                                                  aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTc                                                     <  3:98896/112‑1 (MQ=255)
                                                                                                                                  aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga                >  2:1211551/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga                >  2:883307/1‑149 (MQ=255)
                                                                                                                                   aaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa               >  3:1315073/1‑149 (MQ=255)
                                                                                                                                   aaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa               <  3:481069/149‑1 (MQ=255)
                                                                                                                                   aaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa               >  2:1307986/1‑149 (MQ=255)
                                                                                                                                      aGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagcc            >  1:136100/1‑149 (MQ=255)
                                                                                                                                       gggAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccg           >  2:874834/1‑149 (MQ=255)
                                                                                                                                        ggAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccga          >  1:389786/1‑149 (MQ=255)
                                                                                                                                           aCCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAATGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCcgaagccgaagc       <  4:1569228/149‑1 (MQ=255)
                                                                                                                                            ccATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCt      <  2:439089/149‑1 (MQ=255)
                                                                                                                                             cATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTg     >  4:1458817/1‑149 (MQ=255)
                                                                                                                                              aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt    <  2:389786/149‑1 (MQ=255)
                                                                                                                                              aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt    <  4:255418/149‑1 (MQ=255)
                                                                                                                                              aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt    >  4:752204/1‑149 (MQ=255)
                                                                                                                                              aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt    >  2:928616/1‑149 (MQ=255)
                                                                                                                                               tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccgaa         <  2:669707/143‑1 (MQ=255)
                                                                                                                                               tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccgaa         >  1:669707/1‑143 (MQ=255)
                                                                                                                                               tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGtt   >  4:628407/1‑149 (MQ=255)
                                                                                                                                                cAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTTa  <  1:493445/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTTA  >  NC_000913/2440153‑2440445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: