| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,536,047 | T→G | 100% | F72V (TTT→GTT) | crr → | glucose‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,536,047 | 0 | T | G | 97.2% | 593.2 / 3.4 | 161 | F72V (TTT→GTT) | crr | glucose‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base G (81/75); total (84/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 168 total aligned reads displayed.
TCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGT > NC_000913/2535901‑2536192 | tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCgtt < 3:1487171/149‑1 (MQ=255)tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCgtt < 2:1326737/149‑1 (MQ=255)tCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCgtt < 4:1094333/149‑1 (MQ=255) aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGa < 2:146703/149‑1 (MQ=255) tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGaaa < 4:1199662/149‑1 (MQ=255) cTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAaacc > 2:427393/1‑149 (MQ=255) cTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCG‑TTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAaacca > 1:1214637/1‑149 (MQ=255) tctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCAc > 3:567444/1‑149 (MQ=255) tctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGc > 1:407200/1‑149 (MQ=255) ctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCa < 3:729790/149‑1 (MQ=255) gCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATtc < 3:707781/149‑1 (MQ=255) gaTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTa < 3:134429/149‑1 (MQ=255) aTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTAt < 3:1386458/149‑1 (MQ=255) aTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCCGAAAAAATCATTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCAAt < 4:515515/149‑1 (MQ=255) tCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATc < 1:427393/149‑1 (MQ=255) gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCTGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGa > 2:1029158/1‑149 (MQ=255) gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa > 4:617394/1‑145 (MQ=255) gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGa > 3:571009/1‑149 (MQ=255) cAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAAt > 4:383743/1‑149 (MQ=255) tCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGAt > 4:1529012/1‑149 (MQ=255) tCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGAt > 4:230514/1‑149 (MQ=255) cGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAgcggcg < 4:1057062/149‑1 (MQ=255) tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt < 1:802799/149‑1 (MQ=255) tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt < 3:230514/149‑1 (MQ=255) gATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACt > 1:159942/1‑149 (MQ=255) tCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTc > 1:1105664/1‑149 (MQ=255) gTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt > 4:107194/1‑149 (MQ=255) gTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt > 2:852046/1‑149 (MQ=255) tttttGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTc > 1:1202146/1‑149 (MQ=255) gAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTcc > 1:488701/1‑142 (MQ=255) gAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCgg > 1:341704/1‑149 (MQ=255) aaaaaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGt > 1:679681/1‑149 (MQ=255) ataaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTa < 2:461502/147‑1 (MQ=255) cGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGaca < 2:378295/149‑1 (MQ=255) aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCg > 3:916672/1‑132 (MQ=255) aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCg < 4:916672/132‑1 (MQ=255) tATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGa < 1:1014243/149‑1 (MQ=255) ttGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGaaa < 1:999622/149‑1 (MQ=255) ttGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGaaa < 2:1305722/149‑1 (MQ=255) tGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAg < 4:588694/149‑1 (MQ=255) cTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGc < 4:567444/149‑1 (MQ=255) cTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGc > 1:240242/1‑149 (MQ=255) tATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCg > 2:1532611/1‑149 (MQ=255) tATCAAACCAACGGGTAACAAAATGGTCGCACCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGc > 4:7142/1‑148 (MQ=255) aTCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATc < 2:974329/89‑1 (MQ=255) aTCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATc > 1:974329/1‑89 (MQ=255) aTCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGa > 1:1277101/1‑149 (MQ=255) tCAAACCAACGGGTAACAAAATGGTCGCGCCGGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa > 3:1408405/1‑149 (MQ=255) tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa > 2:556348/1‑149 (MQ=255) tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa > 3:1551405/1‑149 (MQ=255) tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa < 4:839806/149‑1 (MQ=255) tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa > 3:840116/1‑149 (MQ=255) aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc < 4:1488695/123‑1 (MQ=255) aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc > 3:1488695/1‑123 (MQ=255) aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAgg > 2:1214221/1‑149 (MQ=255) aaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGc > 4:673999/1‑149 (MQ=255) ccAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCtt < 3:611060/149‑1 (MQ=255) cAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt < 1:846000/111‑1 (MQ=255) cAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTc > 1:1451960/1‑149 (MQ=255) cGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAg < 3:819343/149‑1 (MQ=255) gggTAACACAATGGTCGCGCCAGTAGACGGCACCCTTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCTACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc < 2:141281/149‑1 (MQ=255) gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc > 3:1464018/1‑149 (MQ=255) gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc < 2:889077/149‑1 (MQ=255) gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACAAACCTCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc > 2:1155486/1‑149 (MQ=255) gTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCAt > 3:256714/1‑63 (MQ=255) gTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCAt < 4:256714/63‑1 (MQ=255) gTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAgcg < 3:334949/84‑1 (MQ=255) tAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTa < 3:1020243/149‑1 (MQ=255) aaCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAt > 4:1303213/1‑149 (MQ=255) aCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGtt < 2:336176/86‑1 (MQ=255) aCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAtt > 3:884602/1‑149 (MQ=255) aCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAtt < 2:198537/149‑1 (MQ=255) aCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAtt > 4:490604/1‑149 (MQ=255) aaaaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc > 4:1364183/1‑108 (MQ=255) aaaaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTg > 2:834828/1‑118 (MQ=255) aaaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCt > 1:25470/1‑149 (MQ=255) aaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTg > 3:1161162/1‑149 (MQ=255) cgcgCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag < 1:675974/149‑1 (MQ=255) cgcgCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag < 3:1031999/149‑1 (MQ=255) cgcgCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag < 1:1158001/149‑1 (MQ=255) gcgcCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag > 4:1144412/1‑148 (MQ=255) gcgcCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag < 3:1144412/148‑1 (MQ=255) gcgcCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAgg > 2:1163870/1‑149 (MQ=255) tAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGaa > 1:93131/1‑71 (MQ=255) gACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt < 4:1408405/149‑1 (MQ=255) gACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt < 3:673999/149‑1 (MQ=255) aCGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc > 3:1200852/1‑83 (MQ=255) ggCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaa > 4:725502/1‑149 (MQ=255) gCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaaa < 4:173223/149‑1 (MQ=255) cATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTg > 2:547628/1‑149 (MQ=255) aTTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAATGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGCCAGCGCGTGAAAGTTgg > 4:1324181/1‑149 (MQ=255) aTTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTgg > 4:20670/1‑149 (MQ=255) aTTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTgg < 2:1187872/149‑1 (MQ=255) ttGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc < 2:240242/149‑1 (MQ=255) ttGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc < 1:852046/149‑1 (MQ=255) ggTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa > 3:130666/1‑102 (MQ=255) aaaaTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATAc < 3:812765/149‑1 (MQ=255) aaaTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACt > 2:1550456/1‑149 (MQ=255) aTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc < 1:1463431/106‑1 (MQ=255) aTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGt < 4:1226796/149‑1 (MQ=255) | TCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGT > NC_000913/2535901‑2536192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |