Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,637,235 T→G 6.5% K41T (AAG→ACG) ‡ der ← GTPase; multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,2350TG 6.5% 578.4 / 27.8 168K41T (AAG→ACG) ‡derGTPase; multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base T (76/81);  new base G (8/3);  total (84/84)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 177 total aligned reads displayed.

ATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCAT  >  NC_000913/2637087‑2637383
                                                                                                                                                    |                                                                                                                                                    
aTCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACt                                                                                                                                                      >  3:1357241/1‑149 (MQ=255)
aTCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACt                                                                                                                                                      >  3:605950/1‑149 (MQ=255)
 tCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACtt                                                                                                                                                     >  4:1052162/1‑149 (MQ=255)
  ccaccaTAAACAGTCCGACGTCCGCTTCTTCAATCGCCAGCAGCGCCTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTa                                                                                                                                                    <  2:1419845/149‑1 (MQ=255)
    accaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACg                                                                                                                                                  >  4:6125/1‑149 (MQ=255)
     ccaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTActg                                                                                                                                                 >  4:1298362/1‑147 (MQ=255)
         aaaCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCAc                                                                                                                                             >  4:916736/1‑149 (MQ=255)
         aaaCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTAAGTACGGTcgt                                                                                                                                             <  2:555437/149‑3 (MQ=255)
           aCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGa                                                                                                                                           <  4:1257437/149‑1 (MQ=255)
           aCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGa                                                                                                                                           >  1:911902/1‑149 (MQ=255)
             aGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGGGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAGCTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGt                                                                                                                                         <  2:705469/149‑1 (MQ=255)
              gTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTc                                                                                                                                        <  3:349774/149‑1 (MQ=255)
               tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCa                                                                                                                                       >  2:54807/1‑149 (MQ=255)
               tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCa                                                                                                                                       >  1:351472/1‑149 (MQ=255)
                      tCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCgg                                                                                                                                >  4:830190/1‑149 (MQ=255)
                      tCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCgg                                                                                                                                <  3:669543/149‑1 (MQ=255)
                         gCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGaa                                                                                                                             >  1:17674/1‑149 (MQ=255)
                            tcttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATc                                                                                                                          >  3:846642/1‑149 (MQ=255)
                            tcttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATc                                                                                                                          <  3:236243/149‑1 (MQ=255)
                            tcttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATc                                                                                                                          >  1:29682/1‑149 (MQ=255)
                             cttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATcc                                                                                                                         <  3:474884/149‑1 (MQ=255)
                             cttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATcc                                                                                                                         <  3:559131/149‑1 (MQ=255)
                             cttcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATcc                                                                                                                         <  2:1393251/149‑1 (MQ=255)
                               tcAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGc                                                                                                                       >  1:317861/1‑149 (MQ=255)
                                      ccAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAgc                                                                                                                >  1:502046/1‑149 (MQ=255)
                                         ggagcGACTGTCCCGCCAGGCGGGTTTCTACACCGCCGTCTGTGCCACCAATCCCGCCCGTATCAATACAGATAAACTCACGGCCTACAATTTCCGCACGACCGTACTTACGGTCACAAGTCAGACCCGGGAAATCCGCAACCAGCGCa                                                                                                             <  4:1348280/147‑1 (MQ=255)
                                              gACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCg                                                                                                        <  2:177841/149‑1 (MQ=255)
                                                    tCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCg                                                                                                  >  1:1517875/1‑149 (MQ=255)
                                                     cctccATGCGGTTTTCTACACCGTCTTCTGTGCCATCGATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGa                                                                                                 <  4:594694/146‑1 (MQ=255)
                                                     ccGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGa                                                                                                 <  1:129756/149‑1 (MQ=255)
                                                       gCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCATCCAGCGCATCTCGGGTGCGAGt                                                                                               <  2:1276541/149‑1 (MQ=255)
                                                        ccATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGtt                                                                                              <  3:1486385/149‑1 (MQ=255)
                                                           tGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                                                                           <  4:361858/149‑1 (MQ=255)
                                                             cGGGTTTCTACCCCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACg                                                                                         <  2:787629/149‑1 (MQ=255)
                                                              gggTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCATACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACgg                                                                                        >  4:571580/1‑149 (MQ=255)
                                                                gTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGtt                                                                                      >  4:643748/1‑149 (MQ=255)
                                                                      acacCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATaa                                                                                >  3:1093212/1‑149 (MQ=255)
                                                                      acacCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATaa                                                                                >  2:188845/1‑149 (MQ=255)
                                                                        acCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACg                                                                              >  1:1173378/1‑149 (MQ=255)
                                                                        acCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACg                                                                              >  1:360784/1‑149 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCTCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGc                                                                                                   >  1:1269146/1‑126 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACTGATAAACACACGGCCTTCAATTTCCGGACGACCGTACTTAc                                                                                                                                                   >  3:979979/1‑78 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGc                                                                                                   <  2:1269146/126‑1 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATaa                                                                                <  3:977650/145‑1 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATaa                                                                                >  4:977650/1‑145 (MQ=255)
                                                                          cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTg                                                                            >  2:1010307/1‑149 (MQ=255)
                                                                                ctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTCCCGCACGACCGTACTTACGGTAACGAGTCAGACCCGGCAAATCCGCAACCAGCGCATCCCGGGTGCGAGTTAGACGGTTAAATAACGTGGAtttt                                                                      >  2:1066898/1‑149 (MQ=255)
                                                                                 tgtgCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTc                                                                     <  2:792490/149‑1 (MQ=255)
                                                                                      cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGt                                                                             <  2:445069/136‑1 (MQ=255)
                                                                                      cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGt                                                                             >  1:445069/1‑136 (MQ=255)
                                                                                       aTCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGt                                                               >  4:151746/1‑149 (MQ=255)
                                                                                        tCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGtt                                                              <  2:351472/149‑1 (MQ=255)
                                                                                          aaTCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAg                                                            <  4:846642/149‑1 (MQ=255)
                                                                                            tCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGGGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGCTTTTCCTACGTTAggc                                                          >  2:1384454/1‑148 (MQ=255)
                                                                                              ccgccgGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGgcg                                                        >  1:341952/1‑149 (MQ=255)
                                                                                                  cgGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCg                                                    >  3:824449/1‑149 (MQ=255)
                                                                                                   ggTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGa                                                   <  3:856062/149‑1 (MQ=255)
                                                                                                       tCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAttt                                                                       <  2:1285019/125‑1 (MQ=255)
                                                                                                       tCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTa                                                                  >  3:1058880/1‑130 (MQ=255)
                                                                                                        cAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgc                                              <  2:911902/149‑1 (MQ=255)
                                                                                                          aTACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgcgc                                            >  4:308350/1‑149 (MQ=255)
                                                                                                           tACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGa                                                                                                 >  4:1005929/1‑95 (MQ=255)
                                                                                                            aCAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGa                                          <  3:902071/149‑1 (MQ=255)
                                                                                                             cAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                         <  2:1173378/149‑1 (MQ=255)
                                                                                                              aGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAcc                                        >  2:590795/1‑149 (MQ=255)
                                                                                                               gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTc                                                                     <  1:163572/119‑1 (MQ=255)
                                                                                                               gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTc                                                                     >  2:163572/1‑119 (MQ=255)
                                                                                                               gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACca                                       <  1:188845/149‑1 (MQ=255)
                                                                                                               gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACca                                       >  2:751085/1‑149 (MQ=255)
                                                                                                                aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAt                                                                         <  3:101443/114‑1 (MQ=255)
                                                                                                                aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcac                                      >  1:395757/1‑149 (MQ=255)
                                                                                                                aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCCGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcac                                      >  4:443407/1‑149 (MQ=255)
                                                                                                                 tAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcaca                                     <  4:985577/149‑1 (MQ=255)
                                                                                                                 tAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcaca                                     <  1:72216/149‑1 (MQ=255)
                                                                                                                 tAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcaca                                     >  2:1515534/1‑149 (MQ=255)
                                                                                                                  aaaCTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAg                                    <  2:1472451/149‑1 (MQ=255)
                                                                                                                     cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTa                                 <  2:951079/149‑1 (MQ=255)
                                                                                                                      tCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACgg                                                                                        <  4:1135916/93‑1 (MQ=255)
                                                                                                                      tCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACgg                                                                                        >  3:1135916/1‑93 (MQ=255)
                                                                                                                      tCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAc                                >  3:1256652/1‑149 (MQ=255)
                                                                                                                         cGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCAt                             <  4:1271004/149‑1 (MQ=255)
                                                                                                                             cTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGttt                         <  2:50438/149‑1 (MQ=255)
                                                                                                                              ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCTTCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTa                        >  4:1074889/1‑149 (MQ=255)
                                                                                                                              ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAgg                                                           >  3:1229454/1‑114 (MQ=255)
                                                                                                                              ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTa                        >  1:1540486/1‑149 (MQ=255)
                                                                                                                              ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCACAACCAGCGCATCTCGGGTGCGAGTTAGACgg                                                                                        >  1:1455754/1‑85 (MQ=255)
                                                                                                                               tCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTaa                       >  1:1577835/1‑149 (MQ=255)
                                                                                                                                 aaTTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAg                     <  2:317861/149‑1 (MQ=255)
                                                                                                                                  aTTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCACCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGc                    <  1:1138079/149‑1 (MQ=255)
                                                                                                                                      ccGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                <  2:1224986/149‑1 (MQ=255)
                                                                                                                                       cGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGcc                   >  3:1271935/1‑145 (MQ=255)
                                                                                                                                        tcgtgACCGTAAGTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTaaa                      <  1:551608/137‑1 (MQ=255)
                                                                                                                                        tcgtgACCGTAAGTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAtt              >  3:220608/5‑149 (MQ=255)
                                                                                                                                          gtgACCGTAAGTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAtttt            >  4:1432581/3‑149 (MQ=255)
                                                                                                                                          gtgACCGTAAGTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAtttt            >  3:732527/3‑149 (MQ=255)
                                                                                                                                          aCGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAtttt            >  3:937767/1‑149 (MQ=255)
                                                                                                                                            gACCGTAAGTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTta          <  4:732527/149‑1 (MQ=255)
                                                                                                                                                   cTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatca   <  4:885310/149‑1 (MQ=255)
                                                                                                                                                    ttACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGCTTAAAGCCTCATTTTTATAAatcat  >  2:48197/1‑149 (MQ=255)
                                                                                                                                                    ttACGGTCACGAGTCAGACCCGGGAAATCCGCAACAGGCGGATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatcat  <  4:1022938/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCAT  >  NC_000913/2637087‑2637383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: