Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,716,030 T→G 6.9% intergenic (+20/+36) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,716,0300TG 6.9% 533.7 / 23.5 156intergenic (+20/+36)eamB/grcAcysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
Reads supporting (aligned to +/- strand):  ref base T (71/74);  new base G (4/7);  total (75/81)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

CGTTTGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATATTTTT  >  NC_000913/2715883‑2716178
                                                                                                                                                   |                                                                                                                                                    
cGTTTGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTc                                                                                                                                                     <  4:779355/149‑1 (MQ=255)
  tttGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGc                                                                                                                                                   <  4:92169/149‑1 (MQ=255)
  tttGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGc                                                                                                                                                   <  2:422346/149‑1 (MQ=255)
  tttGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGc                                                                                                                                                   >  4:345471/1‑149 (MQ=255)
    tGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGccc                                                                                                                                                 <  2:370852/149‑1 (MQ=255)
        aaTGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCtt                                                                                                                                             <  2:1590308/149‑1 (MQ=255)
         aTGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTc                                                                                                                                            >  2:1511691/1‑149 (MQ=255)
          tgtgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTcc                                                                                                                                           <  2:969044/149‑1 (MQ=255)
           gtgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCTTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCg                                                                                                                                          >  4:1130626/1‑149 (MQ=255)
            tgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                                                                         <  2:79192/149‑1 (MQ=255)
            tgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                                                                         >  3:1208079/1‑149 (MQ=255)
            tgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGc                                                                                                                                         <  4:491843/149‑1 (MQ=255)
              tgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCtt                                                                                                                                       >  2:851067/1‑149 (MQ=255)
               gCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTa                                                                                                                                      <  4:206962/149‑1 (MQ=255)
               gCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTa                                                                                                                                      <  4:1090237/149‑1 (MQ=255)
                     cgcTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACt                                                                                                                                >  1:977895/1‑149 (MQ=255)
                       cTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTg                                                                                                                              <  1:1511691/149‑1 (MQ=255)
                        tGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGc                                                                                                                             <  4:728205/149‑1 (MQ=255)
                          gCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTa                                                                                                                           >  3:1247792/1‑149 (MQ=255)
                          gCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTa                                                                                                                           <  3:471922/149‑1 (MQ=255)
                            ggggCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACt                                                                                                                         >  4:455251/1‑149 (MQ=255)
                               gCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTaa                                                                                                                      <  1:72918/149‑1 (MQ=255)
                               gCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTaa                                                                                                                      <  1:301482/149‑1 (MQ=255)
                               gCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTcc                                                                                                                                           <  1:1391615/128‑1 (MQ=255)
                               gCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTcc                                                                                                                                           >  2:1391615/1‑128 (MQ=255)
                                cATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAg                                                                                                                     <  1:685394/149‑1 (MQ=255)
                                 aTCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGc                                                                                                                    >  1:94295/1‑149 (MQ=255)
                                             gATTGTTTCGCCAGTATGGTCGCCTGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCttt                                                                                                        >  1:681755/1‑149 (MQ=255)
                                             gATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCttt                                                                                                        >  3:655341/1‑149 (MQ=255)
                                             gATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCttt                                                                                                        >  3:570163/1‑149 (MQ=255)
                                               ttGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCa                                                                                                      <  3:1130626/149‑1 (MQ=255)
                                               ttGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCa                                                                                                      >  2:715677/1‑149 (MQ=255)
                                                  tttCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCg                                                                                                                                          <  2:164285/110‑1 (MQ=255)
                                                    tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaaa                                                                                                 <  3:299759/149‑1 (MQ=255)
                                                    tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaaa                                                                                                 <  4:869248/149‑1 (MQ=255)
                                                     cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                                                                <  1:337213/149‑1 (MQ=255)
                                                     cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                                                                <  3:459691/149‑1 (MQ=255)
                                                     cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                                                                <  2:977895/149‑1 (MQ=255)
                                                     cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                                                                <  3:677806/149‑1 (MQ=255)
                                                              ggTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCg                                                                                       >  2:951949/1‑149 (MQ=255)
                                                                tCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAt                                                                                     >  1:756263/1‑149 (MQ=255)
                                                                  gCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATaa                                                                                   >  2:1077957/1‑149 (MQ=255)
                                                                  gCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATaa                                                                                   >  2:211223/1‑149 (MQ=255)
                                                                        tAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTcgc                                                                             <  3:345471/149‑1 (MQ=255)
                                                                          aaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTAc                                                                                                               >  2:554656/1‑113 (MQ=255)
                                                                          aaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTAc                                                                                                               <  1:554656/113‑1 (MQ=255)
                                                                           atatCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCa                                                                                                      <  2:305627/121‑1 (MQ=255)
                                                                              tCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaa                                                                                                       <  1:314616/117‑3 (MQ=255)
                                                                                  gCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTc                                                                   <  4:561221/149‑1 (MQ=255)
                                                                                   cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                                                                  <  2:7450/149‑1 (MQ=255)
                                                                                      gCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGc                                                                                        >  4:1105635/1‑124 (MQ=255)
                                                                                      gCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGa                                                               >  2:58906/1‑149 (MQ=255)
                                                                                        ccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt                                                             >  2:358828/1‑149 (MQ=255)
                                                                                        ccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaag                                                                                                      >  3:820584/1‑105 (MQ=255)
                                                                                            ttGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCaga                                                         <  4:1410573/149‑1 (MQ=255)
                                                                                                tGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGt                                                     >  3:1036819/1‑149 (MQ=255)
                                                                                                 ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGtt                                                    <  1:19241/149‑1 (MQ=255)
                                                                                                 ggTCTATTACGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGtt                                                    <  4:734406/149‑1 (MQ=255)
                                                                                                   tCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGa                                                               >  1:1581164/1‑136 (MQ=255)
                                                                                                   tCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGa                                                               <  2:1581164/136‑1 (MQ=255)
                                                                                                   acTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTaa                                                  >  4:1354360/2‑149 (MQ=255)
                                                                                                     tATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAg                                                <  3:149482/149‑1 (MQ=255)
                                                                                                      aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGc                                               <  3:812717/149‑1 (MQ=255)
                                                                                                       ttGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTTCAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCg                                              >  2:206242/1‑149 (MQ=255)
                                                                                                         gcgcGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGaa                                            <  3:224104/149‑1 (MQ=255)
                                                                                                          cgcgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAAc                                           <  1:631080/149‑1 (MQ=255)
                                                                                                           gcgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACt                                          <  2:756263/149‑1 (MQ=255)
                                                                                                               tACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCAt                                      >  2:1397555/1‑149 (MQ=255)
                                                                                                                      tttCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGa                               <  4:1036819/149‑1 (MQ=255)
                                                                                                                          tATTAACGAAAAAAAGCGGAAGTGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATaca                           >  4:1338455/1‑149 (MQ=255)
                                                                                                                          tATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGc                                               >  3:945450/1‑129 (MQ=255)
                                                                                                                          tATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGc                                               <  4:945450/129‑1 (MQ=255)
                                                                                                                            ttAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagaga                                                       <  2:823573/119‑1 (MQ=255)
                                                                                                                              aaCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGa                       >  1:526428/1‑149 (MQ=255)
                                                                                                                               aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCa                                                           <  4:837363/112‑1 (MQ=255)
                                                                                                                               aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGAt                      >  1:82042/1‑149 (MQ=255)
                                                                                                                               aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGAt                      <  2:675475/149‑1 (MQ=255)
                                                                                                                                 gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATgg                    >  1:847363/1‑149 (MQ=255)
                                                                                                                                 gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATgg                    >  2:506185/1‑149 (MQ=255)
                                                                                                                                 gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATgg                    >  1:201379/1‑149 (MQ=255)
                                                                                                                                 gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACGTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATgg                    >  3:1073832/1‑149 (MQ=255)
                                                                                                                                  aaaaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGt                   >  1:1099693/1‑149 (MQ=255)
                                                                                                                                   aaaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTc                  >  1:520308/1‑149 (MQ=255)
                                                                                                                                      aaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGc               >  4:1160723/1‑149 (MQ=255)
                                                                                                                                       aaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                                                                  >  3:851239/1‑97 (MQ=255)
                                                                                                                                       aaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                                                                  <  4:851239/97‑1 (MQ=255)
                                                                                                                                       aaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCt              <  1:1077957/149‑1 (MQ=255)
                                                                                                                                        aGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTg             <  1:546301/149‑1 (MQ=255)
                                                                                                                                        aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGt                                                                                <  1:919770/82‑1 (MQ=255)
                                                                                                                                           ggAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCgg          >  3:1199209/1‑149 (MQ=255)
                                                                                                                                           ggAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCgg          >  3:841412/1‑149 (MQ=255)
                                                                                                                                            gAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGa         >  3:1408353/1‑149 (MQ=255)
                                                                                                                                            gAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGa         <  1:821248/149‑1 (MQ=255)
                                                                                                                                             aaGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGat        >  3:1385433/1‑149 (MQ=255)
                                                                                                                                             aaGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGATACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGat        >  4:1494546/1‑149 (MQ=255)
                                                                                                                                               gagGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGatat      >  3:1111968/1‑149 (MQ=255)
                                                                                                                                                agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGa                               <  2:1550545/123‑1 (MQ=255)
                                                                                                                                                agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGa                               >  1:1550545/1‑123 (MQ=255)
                                                                                                                                                 ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATAttt    <  4:1111968/149‑1 (MQ=255)
                                                                                                                                                   tCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATAttttt  <  3:733053/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTTTGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATATTTTT  >  NC_000913/2715883‑2716178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: