Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,736,914 T→A 5.7% intergenic (+31/‑240) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,736,9140TA 5.7% 488.4 / 15.0 140intergenic (+31/‑240)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base T (75/57);  new base A (2/6);  total (77/63)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 141 total aligned reads displayed.

ACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAA  >  NC_000913/2736767‑2737061
                                                                                                                                                    |                                                                                                                                                   
aCCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTg                                                                                                                                                    >  3:397945/1‑149 (MQ=255)
  ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                  >  1:1074592/1‑149 (MQ=255)
   cAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGc                                                                                                                                                 >  1:1170430/1‑149 (MQ=255)
    aGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCt                                                                                                                                                >  3:1065320/1‑149 (MQ=255)
    aGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCt                                                                                                                                                <  2:1470834/149‑1 (MQ=255)
    aGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCt                                                                                                                                                >  2:1414294/1‑149 (MQ=255)
      gCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGc                                                                                                                                              >  1:1290569/1‑149 (MQ=255)
          cgcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCAAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtt                                                                                                                                          <  1:76829/149‑1 (MQ=255)
           gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttt                                                                                                                                         >  3:625732/1‑149 (MQ=255)
           gcgTGATGAGCTGCGGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACGTAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttt                                                                                                                                         <  4:966302/149‑1 (MQ=255)
             gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                       >  4:535136/1‑149 (MQ=255)
              tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                      <  3:1107205/149‑1 (MQ=255)
              tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                      <  2:820445/149‑1 (MQ=255)
                  gcgcTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTAtt                                                                                                                                  <  1:1053038/149‑1 (MQ=255)
                    gcTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAAACGGCAGCTCAAGGGCTGCCGttttgtgtttc                                                                                                                                 <  2:1487947/149‑8 (MQ=38)
                      tGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                              <  4:1008357/149‑1 (MQ=255)
                       gCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTc                                                                                                                             <  1:815405/149‑1 (MQ=255)
                       gCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTc                                                                                                                             <  1:1414294/149‑1 (MQ=255)
                          gCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGt                                                                                                                          >  2:626524/1‑149 (MQ=255)
                          gCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGt                                                                                                                          >  3:919980/1‑149 (MQ=255)
                            tGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTtg                                                                                                                        >  2:1519929/1‑149 (MQ=255)
                             taTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTtgt                                                                                                                       <  3:136952/148‑1 (MQ=255)
                                ggAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaa                                                                                                                    >  1:425920/1‑149 (MQ=255)
                                 gAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                              >  4:250430/1‑138 (MQ=255)
                                 gAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                              <  3:250430/138‑1 (MQ=255)
                                      tGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGaa                                                                                                              <  4:1422138/149‑1 (MQ=255)
                                      tGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGaa                                                                                                              <  3:1201031/149‑1 (MQ=255)
                                         aTACCGTCAGATGCAGATTAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATCCATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGaa                                                                                                              >  2:1176411/1‑146 (MQ=255)
                                         aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGaa                                                                                                              <  1:1176411/146‑1 (MQ=255)
                                         aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                           >  2:501873/1‑149 (MQ=255)
                                         aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                           >  2:303305/1‑149 (MQ=255)
                                           aCCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAt                                                                                                         <  2:750122/149‑1 (MQ=255)
                                            ccGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAtt                                                                                                        <  4:247323/149‑1 (MQ=255)
                                               tCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaaa                                                                                                                   <  3:542280/135‑1 (MQ=255)
                                                 agatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTc                                                                                                   <  4:1291049/149‑1 (MQ=255)
                                                 agatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTc                                                                                                   <  3:265857/149‑1 (MQ=255)
                                                    tgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                              <  1:736445/119‑1 (MQ=255)
                                                     gcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTa                                                                                               >  1:940888/1‑149 (MQ=255)
                                                     gcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTa                                                                                               >  1:64026/1‑149 (MQ=255)
                                                       agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGCCATCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGCTGTGATACTGAAGCGATTTAGTCGCTATc                                                                                             >  4:548513/1‑149 (MQ=255)
                                                        gatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                            >  2:743651/1‑149 (MQ=255)
                                                          tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                          <  2:940888/149‑1 (MQ=255)
                                                          tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                          <  3:874338/149‑1 (MQ=255)
                                                           gAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtc                                                                                         >  4:899995/1‑149 (MQ=255)
                                                                 gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCATTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                                   >  4:1247297/1‑149 (MQ=255)
                                                                     gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAata                                                                               <  2:64026/149‑1 (MQ=255)
                                                                     gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAata                                                                               >  2:940373/1‑149 (MQ=255)
                                                                      cTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                              <  2:425920/149‑1 (MQ=255)
                                                                             aGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTc                                                                                                                                 <  3:309821/91‑1 (MQ=255)
                                                                             aGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTc                                                                                                                                 >  4:309821/1‑91 (MQ=255)
                                                                                aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCttt                                                                    >  1:137356/1‑149 (MQ=255)
                                                                                 gCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACGAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTg                                                                                                                >  2:841785/1‑104 (MQ=255)
                                                                                 gCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACGAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTg                                                                                                                <  1:841785/104‑1 (MQ=255)
                                                                                 gCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                   <  4:1021147/149‑1 (MQ=255)
                                                                                   gAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                 >  1:506361/1‑149 (MQ=255)
                                                                                    aaaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgaga                                                                >  2:511175/1‑149 (MQ=255)
                                                                                     aaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATgg                                                                            >  4:554353/1‑136 (MQ=255)
                                                                                     aaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATgg                                                                            <  3:554353/136‑1 (MQ=255)
                                                                                           tcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                         >  1:54581/1‑149 (MQ=255)
                                                                                           tcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                         >  2:1268932/1‑149 (MQ=255)
                                                                                              ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                      <  1:911444/149‑1 (MQ=255)
                                                                                              ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                      >  3:1025063/1‑149 (MQ=255)
                                                                                              ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                      <  1:809568/149‑1 (MQ=255)
                                                                                                gcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                    >  3:353870/1‑149 (MQ=255)
                                                                                                          gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAt                                          <  2:1170430/149‑1 (MQ=255)
                                                                                                                 aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAtt                                                                                                        >  1:284472/1‑80 (MQ=255)
                                                                                                                 aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACa                                           >  2:1209455/1‑141 (MQ=255)
                                                                                                                 aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACa                                           <  1:1209455/141‑1 (MQ=255)
                                                                                                                   aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGTTATTCCTCAAGTAAAAAAACATCTATTCCta                                 >  2:952097/1‑148 (MQ=255)
                                                                                                                   aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTg                                 >  3:248393/1‑149 (MQ=255)
                                                                                                                   aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTg                                 >  4:14501/1‑149 (MQ=255)
                                                                                                                    aCAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                  >  3:44347/1‑35 (MQ=255)
                                                                                                                    aCAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                  <  4:44347/35‑1 (MQ=255)
                                                                                                                    aCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGc                                >  2:284223/1‑149 (MQ=255)
                                                                                                                      aGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTc                                    >  2:167438/1‑143 (MQ=255)
                                                                                                                      aGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGa                              >  2:649274/1‑149 (MQ=255)
                                                                                                                       gAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGAt                             <  1:1555606/149‑1 (MQ=255)
                                                                                                                         aaCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGAttt                           >  1:213061/1‑149 (MQ=255)
                                                                                                                          aCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGCTTtc                          >  4:1415215/1‑149 (MQ=255)
                                                                                                                          aCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtc                          <  2:213061/149‑1 (MQ=255)
                                                                                                                           ccTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtct                         <  4:919980/149‑1 (MQ=255)
                                                                                                                             tGAAACACAAAACGGCAGCCCTTGGGCTGCCGATTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                       >  4:313098/1‑149 (MQ=255)
                                                                                                                             tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                       <  2:832323/149‑1 (MQ=255)
                                                                                                                                 acacAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaa                   <  1:649274/149‑1 (MQ=255)
                                                                                                                                   acaAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaa                 >  3:202115/1‑149 (MQ=255)
                                                                                                                                    caAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa                <  4:1164457/149‑1 (MQ=255)
                                                                                                                                    caAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa                <  2:1074592/149‑1 (MQ=255)
                                                                                                                                      aaaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACATAAAAg               >  3:897753/1‑149 (MQ=255)
                                                                                                                                      aaaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAg               >  2:1409536/1‑149 (MQ=255)
                                                                                                                                      aaaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAg               >  1:872705/1‑149 (MQ=255)
                                                                                                                                          cGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTc           <  1:1569318/149‑1 (MQ=255)
                                                                                                                                            gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGt         >  4:389243/1‑149 (MQ=255)
                                                                                                                                            gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGt         >  4:1173232/1‑149 (MQ=255)
                                                                                                                                              aGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg       >  2:956449/1‑149 (MQ=255)
                                                                                                                                                 ccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt             >  3:60656/1‑140 (MQ=255)
                                                                                                                                                 ccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt             <  4:60656/140‑1 (MQ=255)
                                                                                                                                                 ccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa    >  3:62410/1‑149 (MQ=255)
                                                                                                                                                 ccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa    >  1:191737/1‑149 (MQ=255)
                                                                                                                                                  cTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaa   >  2:1007417/1‑149 (MQ=255)
                                                                                                                                                   ttGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaaa  >  4:707561/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAA  >  NC_000913/2736767‑2737061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: