Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,898,541 A→T 6.5% I293N (ATT→AAT)  ygcU ← putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,898,5410AT 6.5% 533.3 / 21.7 155I293N (ATT→AAT) ygcUputative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (75/69);  new base T (3/7);  total (79/76)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-01

Only 100 of 157 total aligned reads displayed.

GAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGC  >  NC_000913/2898396‑2898688
                                                                                                                                                 |                                                                                                                                                    
  ggATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGa                                                                                                                                                 <  3:526464/149‑1 (MQ=255)
   gATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAg                                                                                                                                                <  3:186499/149‑1 (MQ=255)
   gATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAg                                                                                                                                                <  3:927406/149‑1 (MQ=255)
    aTCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAgg                                                                                                                                               <  3:819983/149‑1 (MQ=255)
    aTCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAgg                                                                                                                                               <  1:705542/149‑1 (MQ=255)
     tCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTAACCCTCGCATTGCGAagg                                                                                                                                              <  3:635353/149‑1 (MQ=255)
      cTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGt                                                                                                                                             <  1:1041980/149‑1 (MQ=255)
       tGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                            <  3:1109486/149‑1 (MQ=255)
       tGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                            <  3:975183/149‑1 (MQ=255)
       tGCACACGTTCGGCAGCCACTTTAACCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                            >  2:1428548/1‑149 (MQ=255)
        gCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGTGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTa                                                                                                                                           >  2:112187/1‑149 (MQ=255)
         cacaCGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGt                                                                                                                                             >  3:1373675/1‑146 (MQ=255)
            acGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCtt                                                                                                                                       >  4:1180778/1‑149 (MQ=255)
              gTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCa                                                                                                                                     >  4:1141212/1‑149 (MQ=255)
              gTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCa                                                                                                                                     >  2:1575491/1‑149 (MQ=255)
                      gCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCAACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCACGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATaaa                                                                                                                             <  4:836378/149‑1 (MQ=255)
                       ccACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAg                                                                                                                            >  3:549632/1‑149 (MQ=255)
                       ccACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGATGTCCACGCGCTCGCATTGCGGTTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAg                                                                                                                            >  2:157943/1‑149 (MQ=255)
                           tttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                        <  4:821364/149‑1 (MQ=255)
                            ttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCCATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                        <  4:1489595/148‑1 (MQ=255)
                            ttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                        >  3:1489595/1‑148 (MQ=255)
                               tCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATaaa                                                                                                                             >  4:649145/1‑140 (MQ=255)
                               tCCGGTCCCAAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGc                                                                                                                                   <  3:1147957/134‑1 (MQ=255)
                               tCCGGTCCCAAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGc                                                                                                                                   >  4:1147957/1‑134 (MQ=255)
                                 cGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGc                                                                                                                  >  1:420699/1‑149 (MQ=255)
                                      ccaaGTTCAGGTTGTTAATCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACTTTCGCAATGTGAGGGTTACCTTCAGCCATAAAGATCAGCACGCAtttt                                                                                                             >  4:121145/4‑149 (MQ=255)
                                       ccAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTc                                                                                                           >  3:1162106/1‑149 (MQ=255)
                                       ccAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTc                                                                                                           >  3:863228/1‑149 (MQ=255)
                                       ccAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTc                                                                                                           >  2:1424046/1‑149 (MQ=255)
                                         aGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCg                                                                                                         <  1:1424046/149‑1 (MQ=255)
                                          gTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAgg                                                                                                                                               <  4:415004/111‑1 (MQ=255)
                                          gTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGt                                                                                                        >  3:1119046/1‑149 (MQ=255)
                                          gTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGt                                                                                                        >  3:275773/1‑149 (MQ=255)
                                               ggTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCa                                                                                                   >  2:257818/1‑149 (MQ=255)
                                                  tgttAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCaaaa                                                                                                <  4:1445357/149‑1 (MQ=255)
                                                   gttAAACCAGGATTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAAt                                                                                               >  3:1013666/1‑149 (MQ=255)
                                                    ttAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCGAAATg                                                                                              >  3:90051/1‑149 (MQ=255)
                                                      aaaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATggg                                                                                            <  1:1575491/149‑1 (MQ=255)
                                                       aaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCGCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGt                                                                                           <  2:420699/149‑1 (MQ=255)
                                                       aaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCTAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGt                                                                                           <  2:708715/149‑1 (MQ=255)
                                                       aaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACCCATTTTGCCGACGGAAAATGCGt                                                                                           <  2:1524306/149‑1 (MQ=255)
                                                        aCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTg                                                                                          >  1:306452/1‑149 (MQ=255)
                                                         ccAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGa                                                                                         >  1:1004202/1‑149 (MQ=255)
                                                          cAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                        <  2:132454/149‑1 (MQ=255)
                                                             gTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAgtg                                                                                     >  1:158710/1‑149 (MQ=255)
                                                              tttCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAgtgt                                                                                    >  1:587055/1‑149 (MQ=255)
                                                               ttCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGtt                                                                                   <  1:897739/149‑1 (MQ=255)
                                                                 cGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                 <  4:139647/149‑1 (MQ=255)
                                                                 cGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                 <  2:443616/149‑1 (MQ=255)
                                                                  gATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTggg                                                                                >  1:1081507/1‑149 (MQ=255)
                                                                   aTCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGt                                                                               <  2:629134/149‑1 (MQ=255)
                                                                   aTCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGt                                                                               <  2:193035/149‑1 (MQ=255)
                                                                   aTCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGt                                                                               >  1:527490/1‑149 (MQ=255)
                                                                    tCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                        <  2:67820/108‑1 (MQ=255)
                                                                    tCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                        >  1:67820/1‑108 (MQ=255)
                                                                    tCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTg                                                                              >  4:546382/1‑149 (MQ=255)
                                                                      aGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGcc                                                                            <  1:1428548/149‑1 (MQ=255)
                                                                             tGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGa                                                                                                                                                 >  4:1287714/1‑74 (MQ=255)
                                                                             tGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGa                                                                                                                                                 <  3:1287714/74‑1 (MQ=255)
                                                                                 cACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAg                                                                                       >  1:1514337/1‑127 (MQ=255)
                                                                                  aCGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCACTGCGAGGGTTACCTTCAGCCATAAAGATCAGCCCGCATTTTCCGTCGGCAAAATGGGAGAAGTGTTGGGTGCCGTCTTCAGCGTc                                                                >  2:1247020/1‑149 (MQ=255)
                                                                                  aCGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTc                                                                >  3:349742/1‑149 (MQ=255)
                                                                                         ggCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                 >  3:1546374/1‑125 (MQ=255)
                                                                                         ggCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                 <  4:1546374/125‑1 (MQ=255)
                                                                                          gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                        >  2:1527253/1‑117 (MQ=255)
                                                                                          gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                        <  1:1527253/117‑1 (MQ=255)
                                                                                          gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAAc                                                        >  3:55020/1‑149 (MQ=255)
                                                                                            aTTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGa                                                      >  4:890757/1‑149 (MQ=255)
                                                                                            aTTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGa                                                      <  2:1158126/149‑1 (MQ=255)
                                                                                             ttGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCa                                                               <  3:1416414/139‑1 (MQ=255)
                                                                                             ttGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCa                                                               >  4:1416414/1‑139 (MQ=255)
                                                                                                cGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGa                                                  <  4:1537425/149‑1 (MQ=255)
                                                                                                 gggTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGAt                                                 >  2:376982/1‑149 (MQ=255)
                                                                                                  ggTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGAtt                                                <  2:537193/149‑1 (MQ=255)
                                                                                                     aGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgac                                             <  3:121145/149‑1 (MQ=255)
                                                                                                      gCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacg                                            <  2:327731/149‑1 (MQ=255)
                                                                                                         ggCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac                                         >  4:137205/1‑149 (MQ=255)
                                                                                                         ggCAACGATTTCCGCAATCCCCTCGCCCGCCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgaccgac                                         <  2:1048007/149‑1 (MQ=255)
                                                                                                           cAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGt                                                                                                        <  1:199697/84‑1 (MQ=255)
                                                                                                            aaCGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGAt                                      >  4:1474094/1‑149 (MQ=255)
                                                                                                             aCGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATa                                     >  2:797084/1‑149 (MQ=255)
                                                                                                                 tttCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCt                                 >  1:1463294/1‑149 (MQ=255)
                                                                                                                      gCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTccac                            >  1:301613/1‑149 (MQ=255)
                                                                                                                      gCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTccac                            >  4:565627/1‑149 (MQ=255)
                                                                                                                       cAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTccacc                           <  3:1521607/149‑1 (MQ=255)
                                                                                                                           cccTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGa                       <  2:121552/149‑1 (MQ=255)
                                                                                                                             cTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAtt                     >  3:755488/1‑149 (MQ=255)
                                                                                                                              ttCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt                    >  1:824664/1‑149 (MQ=255)
                                                                                                                                     gTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAg             >  1:799107/1‑149 (MQ=255)
                                                                                                                                     gTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAg             <  4:466902/149‑1 (MQ=255)
                                                                                                                                     gTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAg             <  1:112187/149‑1 (MQ=255)
                                                                                                                                        aCCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGAt          >  3:41949/1‑149 (MQ=255)
                                                                                                                                           ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                        >  1:949652/1‑68 (MQ=255)
                                                                                                                                           ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                        <  2:949652/68‑1 (MQ=255)
                                                                                                                                           ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt       >  2:613883/1‑149 (MQ=255)
                                                                                                                                           ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt       >  2:838657/1‑149 (MQ=255)
                                                                                                                                           ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt       >  3:770712/1‑149 (MQ=255)
                                                                                                                                              gCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa    <  2:800955/149‑1 (MQ=255)
                                                                                                                                                aaTGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGc  >  2:239366/1‑149 (MQ=255)
                                                                                                                                                aaTGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGc  >  1:1446016/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGC  >  NC_000913/2898396‑2898688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: