Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,898,547 G→A 5.3% P291L (CCT→CTT)  ygcU ← putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,898,5470GA 5.3% 503.2 / 11.8 151P291L (CCT→CTT) ygcUputative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (77/66);  new base A (3/5);  total (80/71)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 162 total aligned reads displayed.

GATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTT  >  NC_000913/2898399‑2898695
                                                                                                                                                    |                                                                                                                                                     
gATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAg                                                                                                                                                       <  3:927406/149‑1 (MQ=255)
 aTCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAgg                                                                                                                                                      <  1:705542/149‑1 (MQ=255)
  tCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTAACCCTCGCATTGCGAagg                                                                                                                                                     <  3:635353/149‑1 (MQ=255)
  tCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGACCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAggg                                                                                                                                                     >  1:1488974/1‑149 (MQ=255)
   cTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGt                                                                                                                                                    <  1:1041980/149‑1 (MQ=255)
    tGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                                   <  3:975183/149‑1 (MQ=255)
    tGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                                   <  3:1109486/149‑1 (MQ=255)
    tGCACACGTTCGGCAGCCACTTTAACCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGtt                                                                                                                                                   >  2:1428548/1‑149 (MQ=255)
     gCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGTGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTa                                                                                                                                                  >  2:112187/1‑149 (MQ=255)
      cacaCGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGt                                                                                                                                                    >  3:1373675/1‑146 (MQ=255)
      cacaCGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGACTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGTGAc                                                                                                                                                 <  1:1577593/149‑1 (MQ=255)
       acacGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCCTCGCATTGCGAAGGTGAcg                                                                                                                                                <  3:1517527/149‑2 (MQ=255)
         acGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCtt                                                                                                                                              >  4:1180778/1‑149 (MQ=255)
           gTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCa                                                                                                                                            >  2:1575491/1‑149 (MQ=255)
           gTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCa                                                                                                                                            <  2:1273308/149‑1 (MQ=255)
           gTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCa                                                                                                                                            >  3:203070/1‑149 (MQ=255)
              cGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGcc                                                                                                                                         >  2:41836/1‑149 (MQ=255)
                   gCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCAACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCACGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATaaa                                                                                                                                    <  4:836378/149‑1 (MQ=255)
                    ccACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGATGTCCACGCGCTCGCATTGCGGTTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAg                                                                                                                                   >  2:157943/1‑149 (MQ=255)
                      aCTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGAt                                                                                                                                 <  2:1188174/149‑1 (MQ=255)
                      aCTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGAt                                                                                                                                 <  3:806750/149‑1 (MQ=255)
                        tttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                               >  1:121552/1‑149 (MQ=255)
                        tttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                               <  4:821364/149‑1 (MQ=255)
                         ttATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCCATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                               <  4:1489595/148‑1 (MQ=255)
                            tCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATaaa                                                                                                                                    <  3:649145/140‑1 (MQ=255)
                            tCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATaaa                                                                                                                                    >  4:649145/1‑140 (MQ=255)
                            tCCGGTCCCAAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGc                                                                                                                                          <  3:1147957/134‑1 (MQ=255)
                            tCCGGTCCCAAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGc                                                                                                                                          >  4:1147957/1‑134 (MQ=255)
                                gTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCAt                                                                                                                       <  2:83551/149‑1 (MQ=255)
                                   ccaaGTTCAGGTTGTTAATCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACTTTCGCAATGTGAGGGTTACCTTCAGCCATAAAGATCAGCACGCAtttt                                                                                                                    >  4:121145/4‑149 (MQ=255)
                                    ccAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTc                                                                                                                  >  3:863228/1‑149 (MQ=255)
                                      aGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCg                                                                                                                <  1:1424046/149‑1 (MQ=255)
                                            ggTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCa                                                                                                          >  2:257818/1‑149 (MQ=255)
                                               tgttAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCaaaa                                                                                                       <  4:1445357/149‑1 (MQ=255)
                                                gttAAACCAGGATTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAAt                                                                                                      >  3:1013666/1‑149 (MQ=255)
                                                 ttAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCGAAATg                                                                                                     >  3:90051/1‑149 (MQ=255)
                                                   aaaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATggg                                                                                                   <  1:257818/149‑1 (MQ=255)
                                                   aaaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATggg                                                                                                   <  3:1141212/149‑1 (MQ=255)
                                                    aaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCGCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGt                                                                                                  <  2:420699/149‑1 (MQ=255)
                                                    aaCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACCCATTTTGCCGACGGAAAATGCGt                                                                                                  <  2:1524306/149‑1 (MQ=255)
                                                     aCCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTg                                                                                                 >  1:306452/1‑149 (MQ=255)
                                                      ccAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGa                                                                                                >  1:1004202/1‑149 (MQ=255)
                                                       cAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                               <  4:1119046/149‑1 (MQ=255)
                                                           tttCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAgtgt                                                                                           >  1:587055/1‑149 (MQ=255)
                                                            ttCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGtt                                                                                          <  1:897739/149‑1 (MQ=255)
                                                              cGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                        <  4:139647/149‑1 (MQ=255)
                                                               gATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTggg                                                                                       >  1:1081507/1‑149 (MQ=255)
                                                                aTCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGt                                                                                      <  3:1180778/149‑1 (MQ=255)
                                                                aTCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGt                                                                                      <  2:193035/149‑1 (MQ=255)
                                                                 tCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCa                                                                                                                               <  2:67820/108‑1 (MQ=255)
                                                                 tCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTg                                                                                     >  4:546382/1‑149 (MQ=255)
                                                                           gTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAg                                                                           >  1:905836/1‑149 (MQ=255)
                                                                            tCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGc                                                                          <  2:527490/149‑1 (MQ=255)
                                                                               aCGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTc                                                                       >  3:349742/1‑149 (MQ=255)
                                                                                 gcgcTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCAt                                                                     >  2:133582/1‑149 (MQ=255)
                                                                                   gcTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATAc                                                                   >  1:60272/1‑149 (MQ=255)
                                                                                      ggCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                        <  4:1546374/125‑1 (MQ=255)
                                                                                      ggCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTgg                                                                                        >  3:1546374/1‑125 (MQ=255)
                                                                                       gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                               <  1:1527253/117‑1 (MQ=255)
                                                                                       gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                               >  2:1527253/1‑117 (MQ=255)
                                                                                       gCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAAc                                                               >  3:55020/1‑149 (MQ=255)
                                                                                         aTTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGa                                                             >  4:890757/1‑149 (MQ=255)
                                                                                          ttGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCa                                                                      <  3:1416414/139‑1 (MQ=255)
                                                                                          ttGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAg                                                            >  1:448648/1‑149 (MQ=255)
                                                                                             cGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGa                                                         <  4:1537425/149‑1 (MQ=255)
                                                                                               ggTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGAtt                                                       <  2:537193/149‑1 (MQ=255)
                                                                                                  aGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgac                                                    <  3:1552205/149‑1 (MQ=255)
                                                                                                  aGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgac                                                    <  3:121145/149‑1 (MQ=255)
                                                                                                   gCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacg                                                   <  2:327731/149‑1 (MQ=255)
                                                                                                      ggCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac                                                >  4:137205/1‑149 (MQ=255)
                                                                                                      ggCAACGATTTCCGCAATCCCCTCGCCCGCCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgaccgac                                                <  2:1048007/149‑1 (MQ=255)
                                                                                                        cAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGt                                                                                                               <  1:199697/84‑1 (MQ=255)
                                                                                                         aaCGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCACCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTTAAGTGTTGGGTGCCATCTTCAGCGACATACAAACGAGCGATTGACGGACGAt                                             >  2:649292/1‑149 (MQ=255)
                                                                                                          aCGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATa                                            >  2:797084/1‑149 (MQ=255)
                                                                                                                     aaTCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTccacca                                 >  1:1443103/1‑149 (MQ=255)
                                                                                                                        cccTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGa                              <  2:121552/149‑1 (MQ=255)
                                                                                                                          cTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTcca                                    >  3:1400701/1‑141 (MQ=255)
                                                                                                                          cTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTcca                                    <  4:1400701/141‑1 (MQ=255)
                                                                                                                           ttCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt                           >  1:824664/1‑149 (MQ=255)
                                                                                                                                  gTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAg                    >  1:799107/1‑149 (MQ=255)
                                                                                                                                    aaCCCTCGCATTGCGAAGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGa                  >  4:424856/2‑149 (MQ=255)
                                                                                                                                     aCCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGAt                 >  3:41949/1‑149 (MQ=255)
                                                                                                                                       cTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGt               >  1:15271/1‑149 (MQ=255)
                                                                                                                                        ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGaa                                                                                               <  2:949652/68‑1 (MQ=255)
                                                                                                                                        ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt              >  2:838657/1‑149 (MQ=255)
                                                                                                                                        ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt              >  2:613883/1‑149 (MQ=255)
                                                                                                                                        ttCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGtt              >  3:770712/1‑149 (MQ=255)
                                                                                                                                         tCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTg             <  4:549632/149‑1 (MQ=255)
                                                                                                                                           gCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTCGaa           >  4:146214/1‑149 (MQ=255)
                                                                                                                                             aaTGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGc         >  2:239366/1‑149 (MQ=255)
                                                                                                                                             aaTGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGc         >  1:1446016/1‑149 (MQ=255)
                                                                                                                                               tGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCg       >  3:235993/1‑149 (MQ=255)
                                                                                                                                                gCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTc                                                                       <  3:446528/84‑1 (MQ=255)
                                                                                                                                                gCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTc                                                                       >  4:446528/1‑84 (MQ=255)
                                                                                                                                                gCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCgg      <  4:349742/149‑1 (MQ=255)
                                                                                                                                                  gAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGTCATc                                                                                <  1:1385592/73‑1 (MQ=255)
                                                                                                                                                  gAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGTCATc                                                                                >  2:1385592/1‑73 (MQ=255)
                                                                                                                                                  gAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCa                                                                      >  1:1100105/1‑83 (MQ=255)
                                                                                                                                                  gAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCa                                                                      <  2:1100105/83‑1 (MQ=255)
                                                                                                                                                  gAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtt    <  1:309554/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCCCGTCACCTTCGCAATGCGAGGGTTACCTTCAGCCATAAAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTT  >  NC_000913/2898399‑2898695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: