Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,744 A→T 5.9% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7440AT 5.9% 580.1 / 21.6 168intergenic (‑109/+20)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (75/83);  new base T (5/5);  total (80/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-02

Only 100 of 174 total aligned reads displayed.

ATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTA  >  NC_000913/2980596‑2980892
                                                                                                                                                    |                                                                                                                                                    
aTTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACa                                                                                                                                                      <  1:1186898/149‑1 (MQ=255)
 ttCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAg                                                                                                                                                     >  4:115516/1‑149 (MQ=255)
    ccAGCTCATTCCTCTCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGAAAAACGCCTCAACGGCCAAGCGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                                                  >  3:383817/1‑149 (MQ=255)
    ccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                                                  >  4:169886/1‑149 (MQ=255)
     cAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                                                                                 >  3:704688/1‑149 (MQ=255)
       gCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATc                                                                                                                                               <  2:1095325/149‑1 (MQ=255)
        cTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                                                                              <  3:1213607/149‑1 (MQ=255)
         tCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCg                                                                                                                                             >  1:485818/1‑149 (MQ=255)
          cATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCgg                                                                                                                                            <  2:1049559/149‑1 (MQ=255)
            ttCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTGACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                                                                          <  4:410655/149‑1 (MQ=255)
            ttCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGcc                                                                                                                                          >  2:1417395/1‑149 (MQ=255)
                tccCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTg                                                                                                                                      >  3:854307/1‑149 (MQ=255)
                  ccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaa                                                                                                                                    >  1:834911/1‑149 (MQ=255)
                   cAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCg                                                                                                                                             <  2:1235706/139‑1 (MQ=255)
                    aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                  >  1:1554794/1‑149 (MQ=255)
                    aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                  <  1:1187347/149‑1 (MQ=255)
                    aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                  <  4:1008050/149‑1 (MQ=255)
                     gCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATc                                                                                                                                 <  3:1438246/149‑1 (MQ=255)
                      cAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCa                                                                                                                                <  4:562016/149‑1 (MQ=255)
                       aaaCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaaa                                                                                                                                   >  1:926397/1‑145 (MQ=255)
                       aaaCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                                                                               >  4:1558229/1‑149 (MQ=255)
                        aaCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                                                                              >  4:258311/1‑149 (MQ=255)
                        aaCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                                                                              <  3:1558229/149‑1 (MQ=255)
                            aaTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAATCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGcc                                                                                                                          >  4:904581/1‑149 (MQ=255)
                            aaTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGcc                                                                                                                          >  4:1290995/1‑149 (MQ=255)
                                 ttttCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGatat                                                                                                                     >  4:749661/1‑149 (MQ=255)
                                    tCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtc                                                                                                                  <  2:1545345/149‑1 (MQ=255)
                                     cATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcccttttt                                                                                                                                    >  1:212248/1‑125 (MQ=39)
                                     cATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcccttttt                                                                                                                                    <  2:212248/130‑6 (MQ=39)
                                     cATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtct                                                                                                                 <  4:420876/149‑1 (MQ=255)
                                     cATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtct                                                                                                                 >  4:607859/1‑149 (MQ=255)
                                          ctctctCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACt                                                                                                            >  3:642587/1‑149 (MQ=255)
                                           tctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACtt                                                                                                           >  4:1369867/1‑149 (MQ=255)
                                             tctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtct                                                                                                         >  4:1275922/1‑149 (MQ=255)
                                               tcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                                                                       >  1:305954/1‑149 (MQ=255)
                                               tcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                                                                       >  3:232001/1‑149 (MQ=255)
                                                  tGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCt                                                                                                    >  3:1402434/1‑149 (MQ=255)
                                                  tGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCt                                                                                                    >  3:106379/1‑149 (MQ=255)
                                                    aTGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGc                                                                                                  <  1:407130/149‑1 (MQ=255)
                                                     tgtgtgTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                                                                 <  2:834911/149‑1 (MQ=255)
                                                      gtgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                                                                >  3:748211/1‑149 (MQ=255)
                                                          gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                            <  1:1059189/149‑1 (MQ=255)
                                                             aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGttt                                                                                         <  1:1203685/149‑1 (MQ=255)
                                                              ccGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                                                                        >  3:1525900/1‑149 (MQ=255)
                                                                        gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGatat                                                                              >  1:1160377/1‑149 (MQ=255)
                                                                           taaTAAGCAACGAGACAAACGCCTAAACGGCCAAGTGCCCAATCCCTATTAACAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                                                                           <  4:704688/148‑1 (MQ=255)
                                                                           gAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                                                                           >  1:1011410/1‑149 (MQ=255)
                                                                            aaTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCg                                                                                                                         <  1:979834/102‑1 (MQ=255)
                                                                               aaGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                                                                       >  4:146505/1‑149 (MQ=255)
                                                                               aaGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                                                                       >  4:67042/1‑149 (MQ=255)
                                                                                        gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                                                                              >  1:1467992/1‑85 (MQ=255)
                                                                                        gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                                                                              <  2:1467992/85‑1 (MQ=255)
                                                                                        gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt                                                              <  1:1504277/149‑1 (MQ=255)
                                                                                           aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                           <  1:1417395/149‑1 (MQ=255)
                                                                                           aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                           <  1:27963/149‑1 (MQ=255)
                                                                                           aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                           <  3:42064/149‑1 (MQ=255)
                                                                                           aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                           <  3:1565756/149‑1 (MQ=255)
                                                                                            aaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTg                                                          <  3:67042/149‑1 (MQ=255)
                                                                                            aaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTg                                                          <  3:146505/149‑1 (MQ=255)
                                                                                             aCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTgg                                                         >  3:943074/1‑149 (MQ=255)
                                                                                               gCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCa                                                                                             >  4:335125/1‑111 (MQ=255)
                                                                                                 cTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc                                                     >  1:75877/1‑149 (MQ=255)
                                                                                                 cTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc                                                     >  3:120959/1‑149 (MQ=255)
                                                                                                  tgaaCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc                                                    <  3:1279508/147‑1 (MQ=255)
                                                                                                  tCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc                                                    <  2:1212783/149‑1 (MQ=255)
                                                                                                     aCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGa                                                 >  1:1254280/1‑149 (MQ=255)
                                                                                                      cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                <  2:9277/149‑1 (MQ=255)
                                                                                                      cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                <  4:1402434/149‑1 (MQ=255)
                                                                                                       ggCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAAt                                               <  3:519702/149‑1 (MQ=255)
                                                                                                         ccAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGa                                             <  2:525298/149‑1 (MQ=255)
                                                                                                         ccAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGa                                             <  1:1425777/149‑1 (MQ=255)
                                                                                                            aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGcc                                          <  2:783551/149‑1 (MQ=255)
                                                                                                                 ccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                                                                         >  1:300228/1‑113 (MQ=255)
                                                                                                                 ccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                                                                         <  2:300228/113‑1 (MQ=255)
                                                                                                                 ccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaaa                                     <  3:161105/149‑1 (MQ=255)
                                                                                                                  cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGt                                                        >  2:1545140/1‑129 (MQ=255)
                                                                                                                  cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGt                                                        <  1:1545140/129‑1 (MQ=255)
                                                                                                                  cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg                                    <  4:748211/149‑1 (MQ=255)
                                                                                                                  cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg                                    <  3:1031175/149‑1 (MQ=255)
                                                                                                                     tctcTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTaa                                 <  3:1331870/149‑1 (MQ=255)
                                                                                                                      ctctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                <  4:7108/149‑1 (MQ=255)
                                                                                                                      ctctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                <  4:854942/149‑1 (MQ=255)
                                                                                                                        ctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGc                              <  3:259033/149‑1 (MQ=255)
                                                                                                                         tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGat                                                                                <  4:1302499/98‑1 (MQ=255)
                                                                                                                           ttAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCca                           <  2:808527/149‑1 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                      <  3:868222/149‑1 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                      >  2:1165759/1‑149 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                      >  3:715551/1‑149 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAATTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                      >  4:1496096/1‑149 (MQ=255)
                                                                                                                                 aaaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACg                     >  4:25526/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGc                    >  4:107577/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGc                    >  2:910590/1‑149 (MQ=255)
                                                                                                                                     aaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAt                 <  4:420589/149‑1 (MQ=255)
                                                                                                                                     aaGGGCCGGATGTACAGCACATCCGGCCCGTGAAACCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCCGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAt                 <  2:366684/149‑1 (MQ=255)
                                                                                                                                       gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                <  2:955815/116‑1 (MQ=255)
                                                                                                                                       gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGt               >  2:470328/1‑149 (MQ=255)
                                                                                                                                          ccGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa            <  3:25526/149‑1 (MQ=255)
                                                                                                                                           cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAg           <  4:642587/149‑1 (MQ=255)
                                                                                                                                                 tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgt     <  4:1026596/149‑1 (MQ=255)
                                                                                                                                                    aGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTa  >  2:388614/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTA  >  NC_000913/2980596‑2980892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: