Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,114,881 A→C 6.1% L1411R (CTG→CGG) ‡ sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,8810AC 6.1% 628.2 / 26.8 180L1411R (CTG→CGG) ‡sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base A (90/79);  new base C (5/6);  total (95/85)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 181 total aligned reads displayed.

CTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCG  >  NC_000913/3114733‑3115026
                                                                                                                                                    |                                                                                                                                                 
cTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACa                                                                                                                                                   <  4:844688/149‑1 (MQ=255)
  ccGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAgg                                                                                                                                                 <  4:283795/149‑1 (MQ=255)
  ccGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAgg                                                                                                                                                 <  2:617267/149‑1 (MQ=255)
   cGAAAGATGCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGAGGCATCAACTGGAACAGGt                                                                                                                                                >  2:326235/1‑149 (MQ=255)
   cGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGt                                                                                                                                                >  2:1401200/1‑149 (MQ=255)
   cGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGt                                                                                                                                                >  3:1387982/1‑149 (MQ=255)
   cGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGt                                                                                                                                                >  3:1373615/1‑149 (MQ=255)
    gAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGtt                                                                                                                                               <  3:318296/149‑1 (MQ=255)
         aTCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGc                                                                                                                                          <  4:1242312/149‑1 (MQ=255)
            cGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCACCAACTGGAACATGTTCCAGCCtt                                                                                                                                       <  3:916906/149‑1 (MQ=255)
            cGTCTCGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCTTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGGCCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACCTGTTCCAgttga                                                                                                                                       <  1:828403/149‑5 (MQ=255)
                 gggCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATc                                                                                                                                  <  3:1053119/149‑1 (MQ=255)
                 gggCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATc                                                                                                                                  <  4:1570122/149‑1 (MQ=255)
                  ggCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATcc                                                                                                                                 >  1:895571/1‑149 (MQ=255)
                        ccAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAc                                                                                                                           >  1:879010/1‑149 (MQ=255)
                        ccAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAc                                                                                                                           >  4:570801/1‑149 (MQ=255)
                         cAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcg                                                                                                                          >  3:717859/1‑149 (MQ=255)
                         cAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcg                                                                                                                          <  1:1511164/149‑1 (MQ=255)
                            gaggCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcgctc                                                                                                                       <  2:73534/149‑1 (MQ=255)
                               gCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcgctcgct                                                                                                                    >  4:344864/1‑149 (MQ=255)
                               gCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcgctcgct                                                                                                                    >  1:767104/1‑149 (MQ=255)
                                 acacaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGt                                                                                                                  >  3:371487/1‑149 (MQ=255)
                                 acacaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGt                                                                                                                  >  3:671841/1‑149 (MQ=255)
                                  cacaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCa                                                                                                                                    >  3:1393506/1‑130 (MQ=255)
                                   acaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTaa                                                                                                                >  4:1188085/1‑149 (MQ=255)
                                      gCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAAc                                                                                                             >  2:1485043/1‑149 (MQ=255)
                                        aTCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAActc                                                                                                           >  2:968338/1‑149 (MQ=255)
                                         tCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAActct                                                                                                          >  1:1258173/1‑149 (MQ=255)
                                          cAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTg                                                                                                         <  2:399171/149‑1 (MQ=255)
                                           aGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTgg                                                                                                        <  2:652161/149‑1 (MQ=255)
                                                 tCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagg                                                                                                  >  3:953400/1‑149 (MQ=255)
                                                  ccGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagga                                                                                                 <  2:853519/149‑1 (MQ=255)
                                                    gCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGt                                                                                               <  4:679738/149‑1 (MQ=255)
                                                      tGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGc                                                                                             <  4:1387982/149‑1 (MQ=255)
                                                      tGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCACCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGc                                                                                             >  4:890165/1‑149 (MQ=255)
                                                         gTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCAt                                                                                          >  3:1211252/1‑149 (MQ=255)
                                                               gTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGAt                                                                                    >  4:258444/1‑149 (MQ=255)
                                                                ttGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCTGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATa                                                                                   >  1:1388711/1‑149 (MQ=255)
                                                                ttGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATa                                                                                   <  1:15145/149‑1 (MQ=255)
                                                                 tGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATAc                                                                                  <  4:274675/149‑1 (MQ=255)
                                                                  ggATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATAcc                                                                                 <  3:1169413/149‑1 (MQ=255)
                                                                   gATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCa                                                                                >  4:59416/1‑149 (MQ=255)
                                                                     ttCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                              >  1:647938/1‑149 (MQ=255)
                                                                        aGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCt                                                                           <  2:54609/149‑1 (MQ=255)
                                                                           acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                        <  2:1571286/149‑1 (MQ=255)
                                                                           acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                        <  1:450133/149‑1 (MQ=255)
                                                                           acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                        <  2:12258/149‑1 (MQ=255)
                                                                           acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATACATCAATTTGACCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGCGGAGTGCCATCTGGATACCATTTCTTGa                                                                        <  2:1138353/149‑1 (MQ=255)
                                                                            caGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGat                                                                       <  2:1321088/149‑1 (MQ=255)
                                                                                 aTTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCaa                                                                  >  1:421531/1‑149 (MQ=255)
                                                                                  ttCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCaaa                                                                 <  3:344864/149‑1 (MQ=255)
                                                                                   tCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAg                                                                >  2:1181990/1‑149 (MQ=255)
                                                                                     ttGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGtt                                                              >  2:1315406/1‑149 (MQ=255)
                                                                                        ccgccAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGttttt                                                           <  1:274751/149‑1 (MQ=255)
                                                                                          gccAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTtct                                                         >  1:1581145/1‑149 (MQ=255)
                                                                                            cAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTctct                                                       <  1:1315406/149‑1 (MQ=255)
                                                                                             aaaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTg                                                      >  3:1208839/1‑149 (MQ=255)
                                                                                              aaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGc                                                     >  2:1081548/1‑149 (MQ=255)
                                                                                                cTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGccc                                                   >  4:761334/1‑149 (MQ=255)
                                                                                                      aTTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCt                                             >  1:1340144/1‑149 (MQ=255)
                                                                                                         gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                          <  2:104743/149‑1 (MQ=255)
                                                                                                         gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                          <  2:879010/149‑1 (MQ=255)
                                                                                                         gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                          <  1:748060/149‑1 (MQ=255)
                                                                                                              ccTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgt                                     >  4:549803/1‑149 (MQ=255)
                                                                                                              ccTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgt                                     >  2:1006636/1‑149 (MQ=255)
                                                                                                               cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCGCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                    >  2:1367188/1‑149 (MQ=255)
                                                                                                               cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                    >  4:933240/1‑149 (MQ=255)
                                                                                                               cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                    >  1:1423081/1‑149 (MQ=255)
                                                                                                               cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                    >  3:146500/1‑149 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCt                                                                           >  4:385971/1‑109 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                   <  1:1006636/149‑1 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                   <  4:647126/149‑1 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                   <  2:341960/149‑1 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                   >  4:748953/1‑149 (MQ=255)
                                                                                                                tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                   <  4:1057277/149‑1 (MQ=255)
                                                                                                                 cATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                 >  4:22213/1‑149 (MQ=255)
                                                                                                                  aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagga                                                                                                 >  3:578810/1‑85 (MQ=255)
                                                                                                                  aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATGAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                 >  1:1031919/1‑149 (MQ=255)
                                                                                                                  aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                 <  4:1160269/149‑1 (MQ=255)
                                                                                                                   tCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTa                                >  3:29152/1‑149 (MQ=255)
                                                                                                                        gcgTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATca                           >  4:1008144/1‑149 (MQ=255)
                                                                                                                              tttACGATGCATCAGCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                     <  1:559304/149‑1 (MQ=255)
                                                                                                                              tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCTCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                     <  3:1301541/149‑1 (MQ=255)
                                                                                                                              tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                     >  3:421976/1‑149 (MQ=255)
                                                                                                                              tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGAGATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                     >  2:1309710/1‑149 (MQ=255)
                                                                                                                                  cGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc                 <  1:1309710/149‑1 (MQ=255)
                                                                                                                                        aTCAACTGGATCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGACGATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc           >  4:1363127/1‑149 (MQ=255)
                                                                                                                                        aTCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc           >  2:1464925/1‑149 (MQ=255)
                                                                                                                                         tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCa          >  3:1257723/1‑149 (MQ=255)
                                                                                                                                         tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCa          >  2:1132875/1‑149 (MQ=255)
                                                                                                                                          aggcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                   <  4:871212/136‑1 (MQ=255)
                                                                                                                                          aggcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                   <  4:23778/136‑1 (MQ=255)
                                                                                                                                          aggcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                   >  3:23778/4‑139 (MQ=255)
                                                                                                                                          aggcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                   >  3:871212/4‑139 (MQ=255)
                                                                                                                                           aaCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa                      >  2:1330206/1‑135 (MQ=255)
                                                                                                                                           aaCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa                      <  1:1330206/135‑1 (MQ=255)
                                                                                                                                            gcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCAtt                                                <  4:1453410/107‑1 (MQ=255)
                                                                                                                                              tGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgcc     >  1:1191409/1‑149 (MQ=255)
                                                                                                                                                 aaCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCcgcg  >  3:437367/1‑149 (MQ=255)
                                                                                                                                                  aCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc                 <  2:71011/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCG  >  NC_000913/3114733‑3115026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: