Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,114,882 G→T 6.6% L1411M (CTG→ATG) ‡ sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,8820GT 6.6% 618.6 / 20.5 177L1411M (CTG→ATG) ‡sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base G (88/76);  new base T (5/7);  total (94/83)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 181 total aligned reads displayed.

TCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCC  >  NC_000913/3114734‑3115030
                                                                                                                                                    |                                                                                                                                                    
 ccGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAgg                                                                                                                                                     <  2:617267/149‑1 (MQ=255)
  cGAAAGATGCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGAGGCATCAACTGGAACAGGt                                                                                                                                                    >  2:326235/1‑149 (MQ=255)
  cGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGt                                                                                                                                                    >  2:1401200/1‑149 (MQ=255)
  cGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGt                                                                                                                                                    >  3:1387982/1‑149 (MQ=255)
    aaaGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTc                                                                                                                                                  <  4:82384/149‑1 (MQ=255)
     aaGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCCCAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACCTGTTcc                                                                                                                                                 >  3:1186521/1‑149 (MQ=255)
           cGTCTCGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCTTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGGCCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACCTGTTCCAgttga                                                                                                                                           <  1:828403/149‑5 (MQ=255)
            gTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACCTGTTCCAgttgat                                                                                                                                          <  4:1186521/149‑6 (MQ=255)
                gggCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATc                                                                                                                                      <  3:1053119/149‑1 (MQ=255)
                 ggCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATcc                                                                                                                                     >  1:895571/1‑149 (MQ=255)
                   cGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCt                                                                                                                                   <  4:682716/149‑1 (MQ=255)
                   cGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATACTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCt                                                                                                                                   >  4:1074807/1‑149 (MQ=255)
                       ccAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAc                                                                                                                               >  1:879010/1‑149 (MQ=255)
                        cAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcg                                                                                                                              <  1:1511164/149‑1 (MQ=255)
                           gaggCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcgctc                                                                                                                           <  2:73534/149‑1 (MQ=255)
                              gCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCAcgctcgct                                                                                                                        >  1:767104/1‑149 (MQ=255)
                                acacaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGt                                                                                                                      >  3:671841/1‑149 (MQ=255)
                                 cacaGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCa                                                                                                                                        <  4:1393506/130‑1 (MQ=255)
                                     gCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAAc                                                                                                                 >  2:1485043/1‑149 (MQ=255)
                                       aTCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAActc                                                                                                               >  2:968338/1‑149 (MQ=255)
                                        tCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAActct                                                                                                              >  1:1258173/1‑149 (MQ=255)
                                         cAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTg                                                                                                             <  3:1293117/149‑1 (MQ=255)
                                         cAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTg                                                                                                             <  3:1219857/149‑1 (MQ=255)
                                         cAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTg                                                                                                             <  2:399171/149‑1 (MQ=255)
                                          aGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTgg                                                                                                            <  2:652161/149‑1 (MQ=255)
                                           gCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGc                                                                                                           >  3:1381981/1‑149 (MQ=255)
                                                 ccGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagga                                                                                                     <  2:853519/149‑1 (MQ=255)
                                                     tGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCACCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGc                                                                                                 >  4:890165/1‑149 (MQ=255)
                                                        gTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCAt                                                                                              >  3:274675/1‑149 (MQ=255)
                                                              gTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGAt                                                                                        >  4:258444/1‑149 (MQ=255)
                                                               ttGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCTGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATa                                                                                       >  1:1388711/1‑149 (MQ=255)
                                                               ttGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATa                                                                                       <  1:15145/149‑1 (MQ=255)
                                                                tGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATAc                                                                                      <  4:1211252/149‑1 (MQ=255)
                                                                    ttCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATa                                                                                       <  3:876441/144‑1 (MQ=255)
                                                                    ttCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                                  >  1:647938/1‑149 (MQ=255)
                                                                       aGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCt                                                                               <  2:54609/149‑1 (MQ=255)
                                                                        gCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCtt                                                                              <  4:275126/149‑1 (MQ=255)
                                                                        gCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCtt                                                                              <  3:336352/149‑1 (MQ=255)
                                                                          acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                            <  2:12258/149‑1 (MQ=255)
                                                                          acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                            <  1:450133/149‑1 (MQ=255)
                                                                          acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGa                                                                            <  2:1571286/149‑1 (MQ=255)
                                                                          acaGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATACATCAATTTGACCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGCGGAGTGCCATCTGGATACCATTTCTTGa                                                                            <  2:1138353/149‑1 (MQ=255)
                                                                           caGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGat                                                                           <  2:1321088/149‑1 (MQ=255)
                                                                                aTTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCaa                                                                      >  1:421531/1‑149 (MQ=255)
                                                                                 ttCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCaaa                                                                     <  3:344864/149‑1 (MQ=255)
                                                                                  tCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAg                                                                    >  2:1181990/1‑149 (MQ=255)
                                                                                    ttGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGtt                                                                  >  2:1315406/1‑149 (MQ=255)
                                                                                       ccgccAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGttttt                                                               <  1:274751/149‑1 (MQ=255)
                                                                                         gccAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTtct                                                             >  1:1581145/1‑149 (MQ=255)
                                                                                           cAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTctct                                                           <  1:1315406/149‑1 (MQ=255)
                                                                                            aaaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTg                                                          >  3:1208839/1‑149 (MQ=255)
                                                                                            aaaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTg                                                          >  3:956936/1‑149 (MQ=255)
                                                                                             aaCTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGc                                                         >  2:1081548/1‑149 (MQ=255)
                                                                                                ttGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCa                                                      >  4:190170/1‑149 (MQ=255)
                                                                                                ttGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCa                                                      >  4:13536/1‑149 (MQ=255)
                                                                                                  gTCATTGCTGACCTCATCGCCGCGTGGGTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAATCTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCAtt                                                    <  4:618892/149‑1 (MQ=255)
                                                                                                     aTTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCt                                                 >  1:1340144/1‑149 (MQ=255)
                                                                                                        gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                              <  1:748060/149‑1 (MQ=255)
                                                                                                        gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                              <  3:190170/149‑1 (MQ=255)
                                                                                                        gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                              <  2:104743/149‑1 (MQ=255)
                                                                                                        gCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCa                                              <  2:879010/149‑1 (MQ=255)
                                                                                                             ccTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgt                                         >  4:549803/1‑149 (MQ=255)
                                                                                                             ccTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgt                                         >  2:1006636/1‑149 (MQ=255)
                                                                                                              cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCGCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                        >  2:1367188/1‑149 (MQ=255)
                                                                                                              cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                        >  4:933240/1‑149 (MQ=255)
                                                                                                              cTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                        >  1:1423081/1‑149 (MQ=255)
                                                                                                               tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCt                                                                               <  3:385971/109‑1 (MQ=255)
                                                                                                               tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                       <  1:1006636/149‑1 (MQ=255)
                                                                                                               tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                       >  4:748953/1‑149 (MQ=255)
                                                                                                               tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                       >  3:796983/1‑149 (MQ=255)
                                                                                                               tCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc                                       <  2:341960/149‑1 (MQ=255)
                                                                                                                 aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagga                                                                                                     <  4:578810/85‑1 (MQ=255)
                                                                                                                 aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATGAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                     >  1:1031919/1‑149 (MQ=255)
                                                                                                                 aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                     >  4:479020/1‑149 (MQ=255)
                                                                                                                 aTCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                     <  4:371487/149‑1 (MQ=255)
                                                                                                                       gcgTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATca                               >  4:1008144/1‑149 (MQ=255)
                                                                                                                        cgTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcag                              <  4:25130/149‑1 (MQ=255)
                                                                                                                             tttACGATGCATCAGCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                         <  1:559304/149‑1 (MQ=255)
                                                                                                                             tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                         <  4:146500/149‑1 (MQ=255)
                                                                                                                             tttACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGAGATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc                         >  2:1309710/1‑149 (MQ=255)
                                                                                                                               tACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                       <  3:495996/149‑1 (MQ=255)
                                                                                                                                 cGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc                     <  1:1309710/149‑1 (MQ=255)
                                                                                                                                     gCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCAccc                 <  3:945970/149‑1 (MQ=255)
                                                                                                                                       aTCAACTGGATCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGACGATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc               >  4:1363127/1‑149 (MQ=255)
                                                                                                                                       aTCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc               >  2:1464925/1‑149 (MQ=255)
                                                                                                                                        tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCtt                                                <  4:300578/115‑1 (MQ=255)
                                                                                                                                        tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCtt                                                >  3:300578/1‑115 (MQ=255)
                                                                                                                                        tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCa              >  2:1132875/1‑149 (MQ=255)
                                                                                                                                        tCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCCTGATAACAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCa              >  4:233384/1‑149 (MQ=255)
                                                                                                                                         aggcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                       <  4:871212/136‑1 (MQ=255)
                                                                                                                                          aaCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa                          >  2:1330206/1‑135 (MQ=255)
                                                                                                                                          aaCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa                          <  1:1330206/135‑1 (MQ=255)
                                                                                                                                          aaCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAcc            >  3:430299/1‑149 (MQ=255)
                                                                                                                                           gcTGGAACCTGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCAtt                                                    >  3:1453410/2‑108 (MQ=255)
                                                                                                                                           aCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccg           >  4:651536/1‑149 (MQ=255)
                                                                                                                                           aCTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCACATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccg           >  3:1352519/1‑149 (MQ=255)
                                                                                                                                             tGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgcc         >  1:1191409/1‑149 (MQ=255)
                                                                                                                                                aaCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCcgcg      >  3:649115/1‑149 (MQ=255)
                                                                                                                                                 aCAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc                     <  2:71011/133‑1 (MQ=255)
                                                                                                                                                    ggTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAATACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc  <  3:1074807/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCC  >  NC_000913/3114734‑3115030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: