Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,124,932 G→C 5.0% C176W (TGC→TGG glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,9320GC 5.0% 426.9 / 11.5 121C176W (TGC→TGGglcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base G (63/51);  new base C (2/4);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

Only 100 of 126 total aligned reads displayed.

CCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCG  >  NC_000913/3124785‑3125080
                                                                                                                                                   |                                                                                                                                                    
ccGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGgcgc                                                                                                                                                     >  2:55493/1‑149 (MQ=255)
   tGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGc                                                                                                                                                  <  4:575996/149‑1 (MQ=255)
       aGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTc                                                                                                                                              >  2:1004216/1‑149 (MQ=255)
       aGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              <  1:1063285/149‑3 (MQ=255)
       aGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              <  2:1044353/149‑3 (MQ=255)
         cccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCa                                                                                                                                            >  1:491374/1‑149 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCaa                                                                                                                                           <  1:947905/149‑1 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              <  2:552356/146‑3 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              >  1:552356/1‑144 (MQ=255)
           cTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAAc                                                                                                                                          <  2:1397724/149‑1 (MQ=255)
             ttCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACAt                                                                                                                                        <  3:770988/149‑1 (MQ=255)
                tcCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTaa                                                                                                                                     >  3:829346/1‑149 (MQ=255)
                   tGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAAc                                                                                                                                  >  2:703228/1‑149 (MQ=255)
                     cgcATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAAccc                                                                                                                                <  4:440/149‑1 (MQ=255)
                        aTTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGAc                                                                                                                             >  1:785811/1‑149 (MQ=255)
                         ttAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACg                                                                                                                            >  1:317934/1‑149 (MQ=255)
                             gatgatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTa                                                                                                                        >  4:173380/1‑149 (MQ=255)
                             gatgatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTa                                                                                                                        >  2:305075/1‑149 (MQ=255)
                                gatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATgg                                                                                                                     >  2:844263/1‑149 (MQ=255)
                                gatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATgg                                                                                                                     >  1:448743/1‑149 (MQ=255)
                                gatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATgg                                                                                                                     >  3:121866/1‑149 (MQ=255)
                                 atAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTACCAACATTAAAACCCGACGCTTATGgc                                                                                                                    >  3:323682/1‑149 (MQ=255)
                                    gTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCa                                                                                                                 <  3:315466/149‑1 (MQ=255)
                                     tCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAg                                                                                                                >  1:1375599/1‑149 (MQ=255)
                                     tCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              >  1:1312725/1‑117 (MQ=255)
                                     tCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtac                                                                                                                                              <  2:1312725/119‑3 (MQ=255)
                                       cACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAgcg                                                                                                              >  1:205138/1‑149 (MQ=255)
                                        aCCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAAc                                                                                                                                  >  2:458516/1‑128 (MQ=255)
                                        aCCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAgcgg                                                                                                             >  4:773711/1‑149 (MQ=255)
                                        aCCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAgcgg                                                                                                             >  2:659770/1‑149 (MQ=255)
                                               cacaACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGAcgc                                                                                                      >  4:1547227/1‑149 (MQ=255)
                                                acaacaGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGAcgcg                                                                                                     <  1:1162207/149‑1 (MQ=255)
                                                  aacaGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGt                                                                                                   <  1:766603/149‑1 (MQ=255)
                                                   acaGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGtt                                                                                                  <  2:1375599/149‑1 (MQ=255)
                                                   acaGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGtt                                                                                                  <  4:631880/149‑1 (MQ=255)
                                                    caGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGttt                                                                                                 >  1:1121723/1‑149 (MQ=255)
                                                       ccTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGCCGCGGTTTAGc                                                                                              <  3:1357283/149‑1 (MQ=255)
                                                        cTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCt                                                                                             <  3:173380/149‑1 (MQ=255)
                                                        cTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCt                                                                                             <  1:305075/149‑1 (MQ=255)
                                                         tGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCtt                                                                                            >  1:608739/1‑149 (MQ=255)
                                                           cTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTc                                                                                          <  3:803131/149‑1 (MQ=255)
                                                            ttCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCa                                                                                         >  3:1250556/1‑149 (MQ=255)
                                                             tCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCAc                                                                                        <  3:1547227/149‑1 (MQ=255)
                                                                ttAGCTGGCATGACTCTGCTCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTGGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGt                                                                                     >  2:1058568/1‑149 (MQ=255)
                                                                      ggCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcagc                                                                               <  1:1473516/149‑1 (MQ=255)
                                                                       gCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcagca                                                                              <  4:156731/149‑1 (MQ=255)
                                                                        cATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcagcag                                                                             <  1:1004216/149‑1 (MQ=255)
                                                                        cATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcagcag                                                                             >  3:1554260/1‑149 (MQ=255)
                                                                         aTGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACa                                                                                                                                         <  3:659693/88‑1 (MQ=255)
                                                                          tGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAgcaggc                                                                           >  3:801213/1‑149 (MQ=255)
                                                                           gACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAgcaggca                                                                          <  2:205138/149‑1 (MQ=255)
                                                                              gCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACAt                                                                                                                                        <  2:1511898/84‑1 (MQ=255)
                                                                              gCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACAt                                                                                                                                        >  1:1511898/1‑84 (MQ=255)
                                                                                  aTCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGc                                                                   <  4:1201809/149‑1 (MQ=255)
                                                                                    ccccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctc                                                                 >  2:673382/1‑149 (MQ=255)
                                                                                     cccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGttt                                                                                   <  2:310217/130‑1 (MQ=255)
                                                                                     cccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                                <  2:279504/149‑1 (MQ=255)
                                                                                     cccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                                >  4:1418949/1‑149 (MQ=255)
                                                                                     cccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACACGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                                <  1:1538232/149‑1 (MQ=255)
                                                                                      ccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCgg                                                                                                    <  2:158804/112‑1 (MQ=255)
                                                                                      ccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCgg                                                                                                    >  1:158804/1‑112 (MQ=255)
                                                                                       cAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGa                                                              >  1:886409/1‑149 (MQ=255)
                                                                                        aGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGAc                                                             >  1:1142573/1‑149 (MQ=255)
                                                                                                cAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCg                                                     <  2:1121723/149‑1 (MQ=255)
                                                                                                cAGCACCCGCGCACTTGCCGCGCTGGAGTTGTGCGAAAGCGTAGTCTGGGAACAGCGTTCCAACATTAAAACCTTCCCCTTATATCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCg                                                     >  2:62412/1‑149 (MQ=255)
                                                                                                    aCTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTaa                                                 >  3:1301288/1‑149 (MQ=255)
                                                                                                      tCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTaaaa                                               >  1:697340/1‑149 (MQ=255)
                                                                                                      tCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTaaaa                                               <  2:94189/149‑1 (MQ=255)
                                                                                                        gcgcAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAAc                                             <  2:169027/149‑1 (MQ=255)
                                                                                                        gcgcAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAAc                                             >  1:2984/1‑149 (MQ=255)
                                                                                                        gcgcAGGTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAAc                                             <  2:833385/149‑1 (MQ=255)
                                                                                                          gcAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACgg                                           >  4:808205/1‑149 (MQ=255)
                                                                                                           cAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGt                                          <  2:1312779/149‑1 (MQ=255)
                                                                                                              ttGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCgc                                       >  3:1079818/1‑149 (MQ=255)
                                                                                                               tGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCgca                                      <  2:1037333/149‑1 (MQ=255)
                                                                                                                   gcgTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCAc                                  <  2:780425/149‑1 (MQ=255)
                                                                                                                     gTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCa                                >  1:418865/1‑149 (MQ=255)
                                                                                                                      ttGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAg                               >  4:1220020/1‑149 (MQ=255)
                                                                                                                      ttGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAg                               >  1:401622/1‑149 (MQ=255)
                                                                                                                      ttGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAg                               >  4:1015805/1‑149 (MQ=255)
                                                                                                                         gtgtTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGccc                            >  4:60192/1‑149 (MQ=255)
                                                                                                                           gtTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATg                                                                                                                      <  3:103712/57‑1 (MQ=255)
                                                                                                                           gtTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATg                                                                                                                      >  4:103712/1‑57 (MQ=255)
                                                                                                                              gggCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCt                       >  1:1057086/1‑149 (MQ=255)
                                                                                                                                gCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGc                     <  2:468746/149‑1 (MQ=255)
                                                                                                                                  gAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGt                   >  3:1231250/1‑149 (MQ=255)
                                                                                                                                    aaGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                                <  2:569199/102‑1 (MQ=255)
                                                                                                                                    aaGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                                >  1:569199/1‑102 (MQ=255)
                                                                                                                                      gCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAgc               >  4:1273903/1‑149 (MQ=255)
                                                                                                                                        gTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAgcgc             >  2:475564/1‑149 (MQ=255)
                                                                                                                                          aGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCAc           <  2:886409/149‑1 (MQ=255)
                                                                                                                                          aGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCAc           >  2:647430/1‑149 (MQ=255)
                                                                                                                                            gCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACgg         <  3:77216/149‑1 (MQ=255)
                                                                                                                                             cTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGa        <  2:785811/149‑1 (MQ=255)
                                                                                                                                              tgtgcgcAGCCTTCTAACATTAAAACCCGACGCTCATGGCGCAGCGGCGGACGCGGATTAGATTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTCAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGaa       <  4:1309737/146‑1 (MQ=255)
                                                                                                                                              tGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGaa       <  4:1079818/149‑1 (MQ=255)
                                                                                                                                              tGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGaa       <  4:874565/149‑1 (MQ=255)
                                                                                                                                                 gcgcAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGAc    <  2:1546732/149‑1 (MQ=255)
                                                                                                                                                 gcgcAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGAc    >  1:688023/1‑149 (MQ=255)
                                                                                                                                                   gcAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGAccg  <  1:1494458/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCG  >  NC_000913/3124785‑3125080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: