Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,360,643 T→A 5.8% intergenic (+27/‑533) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,6430TA 5.8% 485.7 / 14.7 137intergenic (+27/‑533)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (55/74);  new base A (3/5);  total (58/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 143 total aligned reads displayed.

ACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATT  >  NC_000913/3360497‑3360790
                                                                                                                                                  |                                                                                                                                                   
aCCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                   <  1:1436821/149‑1 (MQ=255)
  cAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTgtgt                                                                                                                                                 >  4:385920/1‑149 (MQ=255)
    gCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACt                                                                                                                                                     >  3:1502190/1‑143 (MQ=255)
    gCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACt                                                                                                                                                     <  4:1502190/143‑1 (MQ=255)
    gCAACCCGAAAATCTTTGCGGGCGGCGATATCGTCCGTGGTTGCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCt                                                                                                                                               >  3:924651/1‑149 (MQ=255)
       aCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGc                                                                                                                                            <  4:740419/149‑1 (MQ=255)
         ccGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGa                                                                                                                                          >  2:596239/1‑149 (MQ=255)
         ccGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGa                                                                                                                                          >  1:1558375/1‑149 (MQ=255)
           gAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAAt                                                                                                                                        <  3:1519836/149‑1 (MQ=255)
            aaaaTCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATc                                                                                                                                       >  4:51833/1‑149 (MQ=255)
              aaTCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaa                                                                                                                                     >  2:1245094/1‑149 (MQ=255)
               aTCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaaa                                                                                                                                    <  1:1194499/149‑1 (MQ=255)
                tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAAt                                                                                                                                   >  1:552947/1‑149 (MQ=255)
                tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGAAttcatt                                                                                                                                   <  4:267647/149‑6 (MQ=255)
                      cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGa                                                                                                                             <  1:477066/149‑1 (MQ=255)
                      cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGa                                                                                                                             <  2:671706/149‑1 (MQ=255)
                      cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGa                                                                                                                             <  2:676509/149‑1 (MQ=255)
                      cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGa                                                                                                                             <  1:1171888/149‑1 (MQ=255)
                       tGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGAc                                                                                                                            >  3:1530890/1‑149 (MQ=255)
                          cggcgATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGaa                                                                                                                         <  1:1445962/149‑1 (MQ=255)
                           ggcgATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                   <  1:241278/122‑1 (MQ=255)
                           ggcgATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                   >  2:241278/1‑122 (MQ=255)
                           ggcgATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAg                                                                                                                        >  4:426071/1‑149 (MQ=255)
                              gATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTgc                                                                                                                     >  4:206070/1‑149 (MQ=255)
                              gATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTgc                                                                                                                     <  1:1245094/149‑1 (MQ=255)
                                 atCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTAcgcg                                                                                                                  >  3:569974/1‑149 (MQ=255)
                                    gTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGc                                                                                                                                            >  1:1507987/1‑120 (MQ=255)
                                      ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgttgtt                                                                                                             <  4:499542/149‑1 (MQ=255)
                                      ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgttgtt                                                                                                             <  3:206070/149‑1 (MQ=255)
                                           gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTTGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTg                                                                                                        <  2:1331315/149‑1 (MQ=255)
                                           gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTg                                                                                                        <  4:668898/149‑1 (MQ=255)
                                                gATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                                          <  4:690325/142‑1 (MQ=255)
                                                gATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                   >  1:644599/1‑149 (MQ=255)
                                                 aTCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAAt                                                                                                  <  4:669396/149‑1 (MQ=255)
                                                   cTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGc                                                                                                <  3:51833/149‑1 (MQ=255)
                                                     ggtggtGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACAAAGTGCGCGTTGTTTTCTGGCAAATGCgg                                                                                              <  2:474503/149‑1 (MQ=255)
                                                            aCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTCAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTg                                                                                                        >  2:1039008/1‑132 (MQ=255)
                                                            aCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTCAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTg                                                                                                        <  1:1039008/132‑1 (MQ=255)
                                                              cGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                     <  2:552947/149‑1 (MQ=255)
                                                               gCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGtt                                                                                    <  2:1576531/149‑1 (MQ=255)
                                                                cTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGttt                                                                                   <  4:1307773/149‑1 (MQ=255)
                                                                  aTTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCAt                                                                                           <  3:756179/139‑1 (MQ=255)
                                                                  aTTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCAt                                                                                           >  4:756179/1‑139 (MQ=255)
                                                                   ttGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                                                                                                 <  3:915900/100‑1 (MQ=255)
                                                                   ttGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                                                                                                 >  4:915900/1‑100 (MQ=255)
                                                                       cGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCttt                                                                            >  4:1505125/1‑149 (MQ=255)
                                                                        gAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                           >  2:140088/1‑149 (MQ=255)
                                                                        gAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                           <  3:1007811/149‑1 (MQ=255)
                                                                        gAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                           <  1:1511609/149‑1 (MQ=255)
                                                                            gTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaa                                                                                                                                     >  3:74175/1‑87 (MQ=255)
                                                                            gTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGtt                                                                                    <  2:1492578/136‑1 (MQ=255)
                                                                                tAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataata                                                                   <  3:1468025/149‑1 (MQ=255)
                                                                                  aGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                 <  1:1092591/149‑1 (MQ=255)
                                                                                      ggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATa                                                             >  1:957366/1‑149 (MQ=255)
                                                                                          gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAttt                                                         <  4:204046/149‑1 (MQ=255)
                                                                                             ggTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCa                                                      >  3:1094877/1‑149 (MQ=255)
                                                                                              gTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCaa                                                     <  4:170368/149‑1 (MQ=255)
                                                                                               tattatGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAAt                                                    >  3:1322221/1‑149 (MQ=255)
                                                                                                  tatGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATaaa                                                 <  3:1059415/149‑1 (MQ=255)
                                                                                                   atGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAt                                                <  1:621671/149‑1 (MQ=255)
                                                                                                   atGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAt                                                <  2:1456569/149‑1 (MQ=255)
                                                                                                    tGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                                               <  1:140088/149‑1 (MQ=255)
                                                                                                           gctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCttt                                        <  4:1094877/149‑1 (MQ=255)
                                                                                                           gctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCttt                                        <  3:426071/149‑1 (MQ=255)
                                                                                                             tggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                      >  2:366072/1‑149 (MQ=255)
                                                                                                              ggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGtt                                     <  4:231155/149‑1 (MQ=255)
                                                                                                                   tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCa                                >  3:1167831/1‑149 (MQ=255)
                                                                                                                   tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCa                                >  3:356729/1‑149 (MQ=255)
                                                                                                                   tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCa                                >  1:790685/1‑149 (MQ=255)
                                                                                                                      aaGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGt                             <  3:1137396/149‑1 (MQ=255)
                                                                                                                       aGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTc                            >  3:1271694/1‑149 (MQ=255)
                                                                                                                        gCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCt                           >  1:1287459/1‑149 (MQ=255)
                                                                                                                          gAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtga                         <  2:446109/149‑1 (MQ=255)
                                                                                                                           aGGTAACAATGAATTCGCCAGACTGTGTCTGTCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat                        <  2:1531394/149‑1 (MQ=255)
                                                                                                                           aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat                        <  4:924651/149‑1 (MQ=255)
                                                                                                                           aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat                        <  3:385920/149‑1 (MQ=255)
                                                                                                                           aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat                        <  1:26631/149‑1 (MQ=255)
                                                                                                                            ggTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAAGGCGGCATGAGCGTTTGACCTTTCCTATGATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatg                       >  3:986080/1‑149 (MQ=255)
                                                                                                                              tAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAg                     <  1:688145/149‑1 (MQ=255)
                                                                                                                              tAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAg                     >  4:663229/1‑149 (MQ=255)
                                                                                                                               aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCtt                                         <  4:462829/128‑1 (MQ=255)
                                                                                                                                  aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGttt                                                                                   <  3:1464464/83‑1 (MQ=255)
                                                                                                                                  aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAcc                                 <  2:843545/133‑1 (MQ=255)
                                                                                                                                  aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAcc                                 >  1:843545/1‑133 (MQ=255)
                                                                                                                                  aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGaa                 >  3:1391816/1‑149 (MQ=255)
                                                                                                                                    tGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtga                         <  1:1103043/139‑1 (MQ=255)
                                                                                                                                    tGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtga                         >  2:1103043/1‑139 (MQ=255)
                                                                                                                                     gAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGca              <  2:257710/149‑1 (MQ=255)
                                                                                                                                        ttCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACAt           >  2:752541/1‑149 (MQ=255)
                                                                                                                                        ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACAt           >  2:1538095/1‑149 (MQ=255)
                                                                                                                                           gCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa        <  4:508495/149‑1 (MQ=255)
                                                                                                                                           gCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa        <  4:1391816/149‑1 (MQ=255)
                                                                                                                                           gCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa        <  1:1538095/149‑1 (MQ=255)
                                                                                                                                            ccAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAg       >  2:1114566/1‑149 (MQ=255)
                                                                                                                                             cAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGc      >  3:1065518/1‑149 (MQ=255)
                                                                                                                                              aGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataa                                                                     >  4:1062392/1‑85 (MQ=255)
                                                                                                                                              aGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataa                                                                     <  3:1062392/85‑1 (MQ=255)
                                                                                                                                              aGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCa     >  1:290903/1‑149 (MQ=255)
                                                                                                                                                aCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGCGTGAAGCACATCAAGCAAt   <  4:519911/149‑1 (MQ=255)
                                                                                                                                                 cTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAAtt  <  4:767378/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATT  >  NC_000913/3360497‑3360790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: