Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,453,174 C→T 5.4% G33S (GGT→AGT)  rpsJ ← 30S ribosomal subunit protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,1740CT 5.4% 548.7 / 11.0 163G33S (GGT→AGT) rpsJ30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base C (80/74);  new base T (4/5);  total (84/79)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 164 total aligned reads displayed.

TCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCC  >  NC_000913/3453027‑3453322
                                                                                                                                                   |                                                                                                                                                    
 ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAg                                                                                                                                                    >  3:1494684/1‑149 (MQ=255)
  tcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGt                                                                                                                                                   <  3:1248969/149‑1 (MQ=255)
   cGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTg                                                                                                                                                  <  4:491185/149‑1 (MQ=255)
       tGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgca                                                                                                                                              <  2:344320/149‑2 (MQ=255)
        ggCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcag                                                                                                                                             <  4:287856/149‑3 (MQ=255)
        ggCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCtt                                                                                                                                             <  2:303932/149‑1 (MQ=255)
         gCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcagg                                                                                                                                            <  2:618277/149‑4 (MQ=255)
          cTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTgg                                                                                                                                           <  2:229674/149‑1 (MQ=255)
          cTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTgg                                                                                                                                           <  1:1479502/149‑1 (MQ=255)
           tCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcag                                                                                                                                             <  2:494689/146‑3 (MQ=255)
           tCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                                                                                                                                          >  2:944555/1‑149 (MQ=255)
            cAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCa                                                                                                                                         <  4:1566471/149‑1 (MQ=255)
              aCGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGt                                                                                                                                       >  4:631292/1‑149 (MQ=255)
               cGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGtc                                                                                                                                      <  4:1343245/149‑1 (MQ=255)
                  tGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcg                                                                                                                                   >  1:1234318/1‑149 (MQ=255)
                    tCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgac                                                                                                                                 >  3:387645/1‑149 (MQ=255)
                      aaCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga                                                                                                                               >  1:1095692/1‑149 (MQ=255)
                      aaCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga                                                                                                                               >  1:217999/1‑149 (MQ=255)
                      aaCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga                                                                                                                               >  1:436856/1‑149 (MQ=255)
                      aaCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga                                                                                                                               >  1:395257/1‑149 (MQ=255)
                      aaCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga                                                                                                                               >  4:267172/1‑149 (MQ=255)
                       aCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCtt                                                                                                                                             >  3:595912/1‑134 (MQ=255)
                       aCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCtt                                                                                                                                             <  4:595912/134‑1 (MQ=255)
                          aGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTc                                                                                                                           <  4:625462/149‑1 (MQ=255)
                           gACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCtt                                                                                                                                             >  3:1053717/1‑130 (MQ=255)
                             cGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCcgc                                                                                                                        <  1:1167744/149‑1 (MQ=255)
                                aaGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGtct                                                                                                                                     >  1:144156/1‑133 (MQ=255)
                                aaGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGtct                                                                                                                                     <  2:144156/133‑1 (MQ=255)
                                 aGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGtt                                                                                                                    <  1:224972/149‑1 (MQ=255)
                                 aGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGtt                                                                                                                    >  2:313806/1‑149 (MQ=255)
                                       tACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTga                                                                                                              >  1:497202/1‑149 (MQ=255)
                                        aCGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgat                                                                                                             >  3:589153/1‑149 (MQ=255)
                                        aCGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgat                                                                                                             <  2:1172102/149‑1 (MQ=255)
                                         cGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatc                                                                                                            <  3:443425/149‑1 (MQ=255)
                                          ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcg                                                                                                           >  3:1282337/1‑149 (MQ=255)
                                            aTTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                                                         <  2:848321/149‑1 (MQ=255)
                                             tttCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgatc                                                                                                        >  2:1011593/1‑149 (MQ=255)
                                             tttCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgatc                                                                                                        <  4:589153/149‑1 (MQ=255)
                                             tttCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgatc                                                                                                        >  1:1317754/1‑149 (MQ=255)
                                               tCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAg                                                                                                      <  1:71889/149‑1 (MQ=255)
                                                cGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGa                                                                                                     <  2:1551247/149‑1 (MQ=255)
                                                   aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACga                                                                                                  <  3:267172/149‑1 (MQ=255)
                                                     tGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACgatg                                                                                                >  2:1191373/1‑149 (MQ=255)
                                                       aTCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACgatgat                                                                                              <  1:313806/149‑1 (MQ=255)
                                                          gcgcgcgTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCaa                                                                                           >  4:36503/1‑149 (MQ=255)
                                                                    ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAg                                                                                 >  2:403150/1‑149 (MQ=255)
                                                                       ttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCg                                                                              >  1:186038/1‑149 (MQ=255)
                                                                        tgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCgg                                                                             <  4:1494684/149‑1 (MQ=255)
                                                                         gACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGa                                                                            <  1:1541275/149‑1 (MQ=255)
                                                                         gACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGa                                                                            <  1:608619/149‑1 (MQ=255)
                                                                            gTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGATCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                                                         >  1:523851/1‑149 (MQ=255)
                                                                            gTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                                                         >  4:754758/1‑149 (MQ=255)
                                                                               cGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGa                                                                      <  4:672540/149‑1 (MQ=255)
                                                                                  ggAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTc                                                                   >  3:471879/1‑149 (MQ=255)
                                                                                  ggAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCAAGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTc                                                                   <  4:1429020/149‑1 (MQ=255)
                                                                                   gagaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                                                         <  3:1407735/110‑1 (MQ=255)
                                                                                    agaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCtt                                                                 <  4:1282797/149‑1 (MQ=255)
                                                                                    agaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCtt                                                                 <  4:1282337/149‑1 (MQ=255)
                                                                                    agaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCtc                                                                 <  4:1535012/149‑2 (MQ=255)
                                                                                     gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCttt                                                                <  3:1297405/149‑1 (MQ=255)
                                                                                     gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCttt                                                                <  4:272214/149‑1 (MQ=255)
                                                                                     gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCttt                                                                <  2:1876/149‑1 (MQ=255)
                                                                                      aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACgatga                                                                                               >  1:710173/1‑117 (MQ=255)
                                                                                      aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACgatga                                                                                               <  2:710173/117‑1 (MQ=255)
                                                                                      aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTg                                                               >  1:768482/1‑149 (MQ=255)
                                                                                      aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTg                                                               >  1:1265793/1‑149 (MQ=255)
                                                                                      aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTg                                                               >  4:902990/1‑149 (MQ=255)
                                                                                       tCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                                              <  1:843582/149‑1 (MQ=255)
                                                                                       tCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                                              >  3:690299/1‑149 (MQ=255)
                                                                                         aGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                                                            >  2:616450/1‑149 (MQ=255)
                                                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCgg                                                                             <  3:999744/131‑1 (MQ=255)
                                                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                                                           >  4:98748/1‑149 (MQ=255)
                                                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                                                           >  2:188993/1‑149 (MQ=255)
                                                                                              aGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                                                       <  2:1563784/149‑1 (MQ=255)
                                                                                                tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                                                     >  1:488833/1‑149 (MQ=255)
                                                                                                 gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATga                                                    <  3:770127/149‑1 (MQ=255)
                                                                                                 gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATga                                                    <  3:372539/149‑1 (MQ=255)
                                                                                                 gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATga                                                    <  1:644400/149‑1 (MQ=255)
                                                                                                  aaGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATgag                                                   >  1:184195/1‑149 (MQ=255)
                                                                                                      gcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAgg                                                                                <  3:123498/116‑1 (MQ=255)
                                                                                                      gcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTGTCAgg                                                                                >  4:123498/1‑116 (MQ=255)
                                                                                                       ctctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCag                                              >  2:1482413/1‑149 (MQ=255)
                                                                                                          tttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc                                           >  2:107055/1‑149 (MQ=255)
                                                                                                           ttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTg                                                               >  4:373217/1‑128 (MQ=255)
                                                                                                               gtgtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCaa                                      >  2:4296/1‑149 (MQ=255)
                                                                                                                  tCGGCAGCGGGATCGGACCTCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatta                                   >  3:1385576/1‑149 (MQ=255)
                                                                                                                    ggCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGGTTCTTTGGTTCTGCATGAGTCCag                                              <  1:1353170/136‑1 (MQ=255)
                                                                                                                    ggCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCag                                              >  2:1353170/1‑136 (MQ=255)
                                                                                                                       aGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTta                              >  1:1242829/1‑149 (MQ=255)
                                                                                                                         cGGGATCGGACCACGGGCCTGCGCTCTAGTGCGCTTGGAAGTCTCGACGATTTCAGGGGTTGCTTGATCGATCAGACGATGATCAAACGCTGTCAGGTGGATGCGGATTCTTTGGTTCTGCATGAGACCAGATCTCCATTTATTTtata                            >  2:85635/1‑149 (MQ=255)
                                                                                                                                ggACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAg                                                                                 >  3:293034/1‑89 (MQ=255)
                                                                                                                                ggACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGaaa                     >  1:9650/1‑149 (MQ=255)
                                                                                                                                    cACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGa                 >  4:1473481/1‑149 (MQ=255)
                                                                                                                                     aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGAt                <  4:1166933/149‑1 (MQ=255)
                                                                                                                                       ggACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTa              >  2:985683/1‑149 (MQ=255)
                                                                                                                                          caagcgcACTGGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTActc           >  2:1164421/4‑149 (MQ=255)
                                                                                                                                           cTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTActcc          >  3:448285/1‑149 (MQ=255)
                                                                                                                                            tGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                                                           <  3:869671/99‑1 (MQ=255)
                                                                                                                                                cACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGa     <  4:945398/149‑1 (MQ=255)
                                                                                                                                                 aCCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAc    >  1:1558360/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCC  >  NC_000913/3453027‑3453322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: