Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,476,585 G→A 5.7% intergenic (‑145/+22) yheO ← / ← fkpA putative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,476,5850GA 5.7% 410.2 / 8.9 123intergenic (‑145/+22)yheO/fkpAputative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base G (58/58);  new base A (4/3);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 125 total aligned reads displayed.

ACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACA  >  NC_000913/3476437‑3476733
                                                                                                                                                    |                                                                                                                                                    
aCATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTgg                                                                                                                                                      <  4:1041192/149‑1 (MQ=255)
  aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTTATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  4:1389703/1‑149 (MQ=255)
  aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  1:1395463/1‑149 (MQ=255)
  aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  2:539290/1‑149 (MQ=255)
  aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  3:386193/1‑149 (MQ=255)
   tGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCa                                                                                                                                                   <  3:11746/149‑1 (MQ=255)
    gAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAg                                                                                                                                                  >  4:743358/1‑149 (MQ=255)
     aaaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAgg                                                                                                                                                 >  4:668440/1‑149 (MQ=255)
     aaaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAgg                                                                                                                                                 >  3:153328/1‑149 (MQ=255)
      aaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAggc                                                                                                                                                >  3:373173/1‑149 (MQ=255)
          aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            <  1:683202/149‑1 (MQ=255)
          aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            >  2:27060/1‑149 (MQ=255)
          aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            <  2:1263222/149‑1 (MQ=255)
          aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGgcggcg                                                                                                                                            <  1:429999/149‑1 (MQ=255)
            acacTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGt                                                                                                                                          <  3:638969/149‑1 (MQ=255)
             cacTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGtt                                                                                                                                         >  4:379423/1‑149 (MQ=255)
              acTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTc                                                                                                                                        <  3:379423/149‑1 (MQ=255)
               cTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGtttt                                                                                                                                       <  3:11116/149‑3 (MQ=255)
                tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            >  1:780308/1‑143 (MQ=255)
                tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCtt                                                                                                                                      >  3:270687/1‑149 (MQ=255)
                tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCtt                                                                                                                                      >  4:310864/1‑149 (MQ=255)
                  cTTTTCAGGAGCCTGTCGCTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTaa                                                                                                                                    >  2:1261508/1‑149 (MQ=255)
                        aGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTa                                                                                                                              <  3:836716/149‑1 (MQ=255)
                          gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                                                                            <  3:743358/149‑1 (MQ=255)
                          gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                                                                            >  4:1079494/1‑149 (MQ=255)
                              ctctCGTTAACTTTTCAGGGCACGATCATTAATGAGGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTAAATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtttttt                                                                                                                        <  1:1168117/146‑1 (MQ=255)
                               tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  3:1528148/1‑149 (MQ=255)
                               tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:1528148/149‑1 (MQ=255)
                               tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:270687/149‑1 (MQ=255)
                                gTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAg                                                                                                                      >  2:301097/1‑149 (MQ=255)
                                gTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAg                                                                                                                      >  3:905727/1‑149 (MQ=255)
                                 tCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGc                                                                                                                     >  3:1224338/1‑149 (MQ=255)
                                    ttAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGa                                                                                                                  >  3:1170418/1‑149 (MQ=255)
                                         ttttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTg                                                                                                             >  3:1372303/1‑149 (MQ=255)
                                         ttttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTg                                                                                                             >  1:1536403/1‑149 (MQ=255)
                                          tttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            <  4:1229995/149‑1 (MQ=255)
                                                 gCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCGGCTCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTc                                                                                                     <  4:1535882/149‑1 (MQ=255)
                                                     gCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCAt                                                                                                 <  4:466942/149‑1 (MQ=255)
                                                       tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  3:637164/1‑125 (MQ=255)
                                                       tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:637164/125‑1 (MQ=255)
                                                       tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCa                                                                                               >  3:1509769/1‑149 (MQ=255)
                                                             atgatgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTcc                                                                                         <  1:1522377/149‑1 (MQ=255)
                                                                   cGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCttt                                                                                   <  1:539290/149‑1 (MQ=255)
                                                                       tAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCAt                                                                               <  4:373173/149‑1 (MQ=255)
                                                                         aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  3:1135499/149‑1 (MQ=255)
                                                                         aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  2:1072711/149‑1 (MQ=255)
                                                                         aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  3:134228/149‑1 (MQ=255)
                                                                           tAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGc                                                                           >  2:305149/1‑149 (MQ=255)
                                                                              aTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  3:1548905/1‑149 (MQ=255)
                                                                              aTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  3:587944/1‑149 (MQ=255)
                                                                                 aaTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCgg                                                                     <  1:301097/149‑1 (MQ=255)
                                                                                  aTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  4:747127/1‑98 (MQ=255)
                                                                                  aTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  3:747127/98‑1 (MQ=255)
                                                                                    aCAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgcgc                                                                  >  1:387541/1‑149 (MQ=255)
                                                                                      aGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTg                                                                >  1:846507/1‑149 (MQ=255)
                                                                                          gAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGttt                                                            <  4:581768/149‑1 (MQ=255)
                                                                                             gTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCt                                                                                                        <  2:864535/102‑1 (MQ=255)
                                                                                             gTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAAGCTGCGGCt                                                                                                        >  1:864535/1‑102 (MQ=255)
                                                                                                ccGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTTGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATc                                                      <  4:905727/149‑1 (MQ=255)
                                                                                                  gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCttt                                                                                   >  3:1496570/1‑118 (MQ=255)
                                                                                                  gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        <  3:225048/129‑1 (MQ=255)
                                                                                                  gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  4:225048/1‑129 (MQ=255)
                                                                                                  gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca                                                    <  4:1509769/149‑1 (MQ=255)
                                                                                                     ttCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagca                                                 <  3:683420/149‑1 (MQ=255)
                                                                                                      tCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagcag                                                <  1:305149/149‑1 (MQ=255)
                                                                                                         aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTATCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGCTTCACATCCAGCAGctc                                             >  3:625799/1‑149 (MQ=255)
                                                                                                         aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagc                                                  >  3:113688/1‑144 (MQ=255)
                                                                                                         aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagc                                                  <  4:113688/144‑1 (MQ=255)
                                                                                                         aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctc                                             <  2:1536403/149‑1 (MQ=255)
                                                                                                          gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt                                                             >  3:381500/1‑132 (MQ=255)
                                                                                                          gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATcc                                                     >  2:784355/1‑140 (MQ=255)
                                                                                                          gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAAAGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt                                                             <  4:381500/132‑1 (MQ=255)
                                                                                                           cACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTAAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            <  2:983904/148‑1 (MQ=255)
                                                                                                            aCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTAc                                          <  3:182382/149‑1 (MQ=255)
                                                                                                            aCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTAc                                          <  1:1524956/149‑1 (MQ=255)
                                                                                                              tAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            <  1:1251240/145‑1 (MQ=255)
                                                                                                                 ttATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCaa                                     <  1:826514/149‑1 (MQ=255)
                                                                                                                  tataTCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCaaa                                    <  4:386193/149‑1 (MQ=255)
                                                                                                                    tatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAaca                                  <  2:1037322/149‑1 (MQ=255)
                                                                                                                    tatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAaca                                  <  2:387541/149‑1 (MQ=255)
                                                                                                                     atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                 >  3:87277/1‑149 (MQ=255)
                                                                                                                     atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                 >  2:46425/1‑149 (MQ=255)
                                                                                                                       ccGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCa                               <  4:1170418/149‑1 (MQ=255)
                                                                                                                        cGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAg                              <  4:153328/149‑1 (MQ=255)
                                                                                                                        cGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAg                              >  3:895490/1‑149 (MQ=255)
                                                                                                                           ccTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGt                           <  1:880498/149‑1 (MQ=255)
                                                                                                                              gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca                                                    >  2:1265162/1‑121 (MQ=255)
                                                                                                                                  taaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTc                    >  4:115236/1‑149 (MQ=255)
                                                                                                                                   aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCg                   >  4:1440500/1‑149 (MQ=255)
                                                                                                                                    aaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCgg                  >  2:388623/1‑149 (MQ=255)
                                                                                                                                    aaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCgg                  >  4:862186/1‑149 (MQ=255)
                                                                                                                                     aaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGt                 <  4:1469740/149‑1 (MQ=255)
                                                                                                                                      aaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            >  1:1014122/1‑57 (MQ=255)
                                                                                                                                      aaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            <  2:1014122/57‑1 (MQ=255)
                                                                                                                                         aCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTACATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGa             >  3:1283686/1‑149 (MQ=255)
                                                                                                                                          ccgccgCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGAt            >  1:36619/1‑149 (MQ=255)
                                                                                                                                          ccgccgCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGAt            >  2:1000943/1‑149 (MQ=255)
                                                                                                                                           cgccgcCTGACAAGGCGGCGGTTTTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATc           >  1:1180276/1‑149 (MQ=255)
                                                                                                                                                ccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCgg      >  2:327754/1‑149 (MQ=255)
                                                                                                                                                    gTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAaca  >  4:253364/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACA  >  NC_000913/3476437‑3476733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: