Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,476,586 T→C 5.5% intergenic (‑146/+21) yheO ← / ← fkpA putative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,476,5860TC 5.5% 408.1 / 8.5 124intergenic (‑146/+21)yheO/fkpAputative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base T (59/58);  new base C (4/3);  total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 124 total aligned reads displayed.

ATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACA  >  NC_000913/3476439‑3476733
                                                                                                                                                   |                                                                                                                                                   
aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTTATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  4:1389703/1‑149 (MQ=255)
aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  2:539290/1‑149 (MQ=255)
aTGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                                                                    >  3:386193/1‑149 (MQ=255)
 tGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCa                                                                                                                                                   <  3:11746/149‑1 (MQ=255)
  gAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAg                                                                                                                                                  >  4:743358/1‑149 (MQ=255)
   aaaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAgg                                                                                                                                                 >  4:668440/1‑149 (MQ=255)
   aaaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAgg                                                                                                                                                 >  3:153328/1‑149 (MQ=255)
    aaaaaaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAggc                                                                                                                                                >  3:373173/1‑149 (MQ=255)
        aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            <  1:683202/149‑1 (MQ=255)
        aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            >  2:27060/1‑149 (MQ=255)
        aaaCACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            <  2:1263222/149‑1 (MQ=255)
          acacTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGt                                                                                                                                          <  3:638969/149‑1 (MQ=255)
           cacTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGtt                                                                                                                                         >  4:379423/1‑149 (MQ=255)
            acTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTc                                                                                                                                        <  3:379423/149‑1 (MQ=255)
             cTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGtttt                                                                                                                                       <  3:11116/149‑3 (MQ=255)
              tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            >  1:780308/1‑143 (MQ=255)
              tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGgcggcg                                                                                                                                            <  2:780308/143‑1 (MQ=255)
              tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCtt                                                                                                                                      >  4:310864/1‑149 (MQ=255)
              tCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCtt                                                                                                                                      >  3:270687/1‑149 (MQ=255)
                 ttttCAGGAGCTTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACGAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAAt                                                                                                                                   >  4:1078334/1‑149 (MQ=255)
                 ttttCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAAt                                                                                                                                   >  3:1178893/1‑149 (MQ=255)
                      aGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTa                                                                                                                              <  3:836716/149‑1 (MQ=255)
                        gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                                                                            <  3:743358/149‑1 (MQ=255)
                        gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                                                                            >  4:1079494/1‑149 (MQ=255)
                            ctctCGTTAACTTTTCAGGGCACGATCATTAATGAGGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTAAATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtttttt                                                                                                                        <  1:1168117/146‑1 (MQ=255)
                             tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:270687/149‑1 (MQ=255)
                             tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:1528148/149‑1 (MQ=255)
                             tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  3:581768/1‑149 (MQ=255)
                             tGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  3:1528148/1‑149 (MQ=255)
                               tCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGc                                                                                                                     >  3:1224338/1‑149 (MQ=255)
                                  ttAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGa                                                                                                                  >  3:1170418/1‑149 (MQ=255)
                                       ttttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTg                                                                                                             >  3:1372303/1‑149 (MQ=255)
                                       ttttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTg                                                                                                             >  1:1536403/1‑149 (MQ=255)
                                        tttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            <  4:1229995/149‑1 (MQ=255)
                                         ttCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCg                                                                                                           <  3:845306/149‑1 (MQ=255)
                                               gCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCGGCTCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTc                                                                                                     <  4:1535882/149‑1 (MQ=255)
                                                   gCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCAt                                                                                                 <  1:427552/149‑1 (MQ=255)
                                                   gCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCAt                                                                                                 <  4:466942/149‑1 (MQ=255)
                                                     tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       <  4:637164/125‑1 (MQ=255)
                                                         tAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCtt                                                                                           >  3:1469740/1‑149 (MQ=255)
                                                           atgatgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTcc                                                                                         <  1:1522377/149‑1 (MQ=255)
                                                                gCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCtt                                                                                    <  3:668440/149‑1 (MQ=255)
                                                                 cGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCttt                                                                                   <  1:539290/149‑1 (MQ=255)
                                                                     tAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCAt                                                                               <  4:373173/149‑1 (MQ=255)
                                                                       aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  3:1135499/149‑1 (MQ=255)
                                                                       aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  2:1072711/149‑1 (MQ=255)
                                                                       aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa                                                                             <  3:134228/149‑1 (MQ=255)
                                                                         tAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGc                                                                           >  2:305149/1‑149 (MQ=255)
                                                                            aTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  3:1548905/1‑149 (MQ=255)
                                                                            aTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  3:587944/1‑149 (MQ=255)
                                                                                aTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                                                                       >  4:747127/1‑98 (MQ=255)
                                                                                  aCAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgcgc                                                                  >  1:387541/1‑149 (MQ=255)
                                                                                    aGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTg                                                                >  1:846507/1‑149 (MQ=255)
                                                                                        gAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGttt                                                            <  4:581768/149‑1 (MQ=255)
                                                                                         aGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTc                                                           <  4:1224338/149‑1 (MQ=255)
                                                                                           gTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAAGCTGCGGCt                                                                                                        >  1:864535/1‑102 (MQ=255)
                                                                                              ccGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTTGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATc                                                      <  4:905727/149‑1 (MQ=255)
                                                                                                gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCttt                                                                                   >  3:1496570/1‑118 (MQ=255)
                                                                                                gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                                                                        >  4:225048/1‑129 (MQ=255)
                                                                                                gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca                                                    <  4:1509769/149‑1 (MQ=255)
                                                                                                   ttCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagca                                                 <  3:683420/149‑1 (MQ=255)
                                                                                                    tCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagcag                                                <  1:305149/149‑1 (MQ=255)
                                                                                                     ccAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCagcagc                                               <  3:310864/149‑1 (MQ=255)
                                                                                                      cAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGct                                              >  3:1533489/1‑149 (MQ=255)
                                                                                                       aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTATCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGCTTCACATCCAGCAGctc                                             >  3:625799/1‑149 (MQ=255)
                                                                                                       aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcagc                                                  <  4:113688/144‑1 (MQ=255)
                                                                                                       aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctc                                             >  3:1113251/1‑149 (MQ=255)
                                                                                                       aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctc                                             <  2:1536403/149‑1 (MQ=255)
                                                                                                        gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATcc                                                     >  2:784355/1‑140 (MQ=255)
                                                                                                        gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATcc                                                     <  1:784355/140‑1 (MQ=255)
                                                                                                        gCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAAAGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt                                                             <  4:381500/132‑1 (MQ=255)
                                                                                                         cACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTAAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            <  2:983904/148‑1 (MQ=255)
                                                                                                         cACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTAAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            >  1:983904/1‑148 (MQ=255)
                                                                                                          aCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTAc                                          <  3:182382/149‑1 (MQ=255)
                                                                                                          aCTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTAc                                          <  1:1524956/149‑1 (MQ=255)
                                                                                                            tAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            >  2:1251240/1‑145 (MQ=255)
                                                                                                            tAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGctct                                            <  1:1251240/145‑1 (MQ=255)
                                                                                                               ttATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCaa                                     >  1:1555819/1‑149 (MQ=255)
                                                                                                                tataTCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCaaa                                    <  4:386193/149‑1 (MQ=255)
                                                                                                                  tatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAaca                                  <  2:387541/149‑1 (MQ=255)
                                                                                                                  tatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAaca                                  <  2:1037322/149‑1 (MQ=255)
                                                                                                                   atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                 >  2:46425/1‑149 (MQ=255)
                                                                                                                   atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                 >  3:87277/1‑149 (MQ=255)
                                                                                                                      cGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAg                              <  4:153328/149‑1 (MQ=255)
                                                                                                                      cGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAg                              >  3:895490/1‑149 (MQ=255)
                                                                                                                      cGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAg                              <  2:381545/149‑1 (MQ=255)
                                                                                                                         ccTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGt                           <  1:880498/149‑1 (MQ=255)
                                                                                                                            gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca                                                    >  2:1265162/1‑121 (MQ=255)
                                                                                                                            gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca                                                    <  1:1265162/121‑1 (MQ=255)
                                                                                                                                taaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTc                    >  4:115236/1‑149 (MQ=255)
                                                                                                                                 aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCg                   >  4:1440500/1‑149 (MQ=255)
                                                                                                                                  aaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCgg                  >  4:862186/1‑149 (MQ=255)
                                                                                                                                   aaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGt                 <  4:1469740/149‑1 (MQ=255)
                                                                                                                                    aaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            <  2:1014122/57‑1 (MQ=255)
                                                                                                                                    aaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGc                                                                                                            >  1:1014122/1‑57 (MQ=255)
                                                                                                                                    aaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTg                <  2:1555819/149‑1 (MQ=255)
                                                                                                                                        ccgccgCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGAt            >  1:36619/1‑149 (MQ=255)
                                                                                                                                         cgccgcCTGACAAGGCGGCGGTTTTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATc           >  1:1180276/1‑149 (MQ=255)
                                                                                                                                              ccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCgg      >  2:327754/1‑149 (MQ=255)
                                                                                                                                                  gTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAaca  >  4:253364/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACA  >  NC_000913/3476439‑3476733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: