Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,722,300 G→T 5.3% intergenic (+195/+28) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,722,3000GT 5.3% 2409.6 / 81.9 675intergenic (+195/+28)sokA/glySpseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (331/307);  new base T (22/14);  total (354/321)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

Only 100 of 685 total aligned reads displayed.

CCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAA  >  NC_000913/3722153‑3722448
                                                                                                                                                   |                                                                                                                                                    
ccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGa                                                                                                                                                     >  2:327219/1‑149 (MQ=255)
  gTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATg                                                                                                                                                   <  2:846970/149‑1 (MQ=255)
     gCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCa                                                                                                                                                >  2:1393913/1‑149 (MQ=255)
     gCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCa                                                                                                                                                <  3:486341/149‑1 (MQ=255)
     gCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCa                                                                                                                                                <  3:58376/149‑1 (MQ=255)
     gCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCa                                                                                                                                                <  3:886213/149‑1 (MQ=255)
         ttCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAGAAGCCTGCCAGATGGCAGGCt                                                                                                                                            >  1:1590423/1‑149 (MQ=255)
            tAGCTTAATAAGTTTTTTCCTTCTCCTTGATATAATCCCAAAAAAATCTGATAGAATTACTATATGAGATAATCTTTATTCAGCAAAAGATTATTAAAGGTTTCGGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTagt                                                                                                                                         >  4:1347402/1‑147 (MQ=255)
            tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCtttt                                                                                                                                         <  1:183539/149‑1 (MQ=255)
            tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAtt                                                                                                                                         >  4:162385/1‑149 (MQ=255)
            tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAtt                                                                                                                                         <  1:918221/149‑1 (MQ=255)
            tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAtt                                                                                                                                         <  1:398723/149‑1 (MQ=255)
             aGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAttt                                                                                                                                        >  1:859906/1‑149 (MQ=255)
               cTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                      <  1:1179476/149‑1 (MQ=255)
                  aataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGAt                                                                                                                                                    <  1:1369674/132‑1 (MQ=255)
                  aataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaataa                                                                                                                                   <  3:91242/149‑1 (MQ=255)
                    taaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACg                                                                                                                                 >  3:698718/1‑149 (MQ=255)
                       gtttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCg                                                                                                                              >  4:419264/1‑149 (MQ=255)
                        tttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATgg                                                                                                                                                  <  1:553361/128‑1 (MQ=255)
                        tttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGt                                                                                                                             >  1:254499/1‑149 (MQ=255)
                        tttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGt                                                                                                                             >  3:1379522/1‑149 (MQ=255)
                            tttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatt                                                                                                                         >  1:1224093/1‑149 (MQ=255)
                            tttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATCAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGc                                                                                                                                                 >  3:445395/1‑125 (MQ=255)
                               cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCt                                                                                                                                            <  1:947502/127‑1 (MQ=255)
                               cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTattatt                                                                                                                      <  4:1261430/149‑1 (MQ=255)
                                   tcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCATAAGATGATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCaa                                                                                                                  >  2:1265466/1‑149 (MQ=255)
                                   tcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCaa                                                                                                                  <  3:383913/149‑1 (MQ=255)
                                   tcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCaa                                                                                                                  >  1:1409570/1‑149 (MQ=255)
                                     cTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                                <  3:1191637/149‑1 (MQ=255)
                                       tGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGc                                                                                                              <  4:503460/149‑1 (MQ=255)
                                        gATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaat                                                                                                                                     <  2:472221/125‑1 (MQ=255)
                                         atatAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGa                                                                                                            >  1:631951/1‑149 (MQ=255)
                                          tataATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGaa                                                                                                           <  1:696798/149‑1 (MQ=255)
                                           ataATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGaaa                                                                                                          <  1:327219/149‑1 (MQ=255)
                                             aaTCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAata                                                                                                        >  3:948475/1‑149 (MQ=255)
                                               tCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATc                                                                                                      >  4:594796/1‑149 (MQ=255)
                                               tCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATc                                                                                                      <  2:631951/149‑1 (MQ=255)
                                                   aaaaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGCCGttattaaata                                                                                                                    <  2:1584185/131‑5 (MQ=255)
                                                     aaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTattatt                                                                                                                      <  1:749963/127‑1 (MQ=255)
                                                     aaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGCCGttattaaata                                                                                                                    >  2:1002185/1‑125 (MQ=255)
                                                        aaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTTTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAAcgcg                                                                                             <  2:258728/149‑1 (MQ=255)
                                                        aaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAAcgcg                                                                                             <  2:1452516/149‑1 (MQ=255)
                                                          tctcATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCa                                                                                           <  2:1346470/149‑1 (MQ=255)
                                                              aTAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGaa                                                                                       <  4:348668/149‑1 (MQ=255)
                                                              aTAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGACAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAg                                                                                          >  1:590920/1‑146 (MQ=255)
                                                              aTAAAATTAATATATGAGAGAATCTTAATTCAGCAGAAGATTATTAAAGGTTGCTGTGTTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTACTAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGTAGGaa                                                                                       >  2:109000/1‑149 (MQ=255)
                                                                aaaaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCt                                                                                                                                            <  3:782000/94‑1 (MQ=255)
                                                                   aTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGtt                                                                                  >  1:1497672/1‑149 (MQ=255)
                                                                    ttAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTc                                                                                 >  1:1443677/1‑149 (MQ=255)
                                                                           atGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGt                                                                          <  2:762988/149‑1 (MQ=255)
                                                                            tGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                         <  3:848648/149‑1 (MQ=255)
                                                                            tGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                         >  3:150617/1‑149 (MQ=255)
                                                                            tGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                         >  4:683187/1‑149 (MQ=255)
                                                                             gagaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGttt                                                                        >  1:446660/1‑149 (MQ=255)
                                                                                aTAATCTTTATTCAGCATAAGATGATTATAGGTTGCTGTATTATTTATCGATAAAAAAAGACTGCGAGATGGTAGGCTATTTAATAACGGCGTTATTATTGCAACAGAGAAATATCCGCAACGCGCAGAACTAGTTCGAGCAGTTtctc                                                                     >  2:584496/1‑149 (MQ=255)
                                                                                aTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGaa                                                                                       >  4:450926/1‑131 (MQ=255)
                                                                                aTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtctc                                                                     <  2:410958/149‑1 (MQ=255)
                                                                                 tAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                    >  2:1196480/1‑149 (MQ=255)
                                                                                 tAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                    <  3:277046/149‑1 (MQ=255)
                                                                                      tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatta                                                                                                                        >  4:906315/1‑92 (MQ=255)
                                                                                         aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGt                                                            <  4:1518486/149‑1 (MQ=255)
                                                                                              gcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGAc                                                       >  2:978942/1‑149 (MQ=255)
                                                                                              gcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGAc                                                       <  4:1410666/149‑1 (MQ=255)
                                                                                                  aagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATg                                                              <  2:1038404/138‑1 (MQ=255)
                                                                                                     attattAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                                >  4:1174468/1‑149 (MQ=255)
                                                                                                      ttattaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATa                                               <  1:1131939/149‑1 (MQ=255)
                                                                                                         ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGc                                            <  1:797013/149‑1 (MQ=255)
                                                                                                         ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGc                                            >  4:757159/1‑149 (MQ=255)
                                                                                                           aaaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCaa                                          >  1:1001048/1‑149 (MQ=255)
                                                                                                            aaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAAt                                         >  3:131303/1‑149 (MQ=255)
                                                                                                               gTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGt                                                                                   >  3:356502/1‑104 (MQ=255)
                                                                                                               gTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGCCAGACGGTTGATACGCAATTCt                                      <  2:1133147/149‑1 (MQ=255)
                                                                                                                   cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGt                                  <  4:839599/149‑1 (MQ=255)
                                                                                                                      tattatTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTg                                   >  2:661409/1‑145 (MQ=255)
                                                                                                                      tattatTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcat                               >  3:806665/1‑149 (MQ=255)
                                                                                                                      tattatTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcat                               >  3:218590/1‑149 (MQ=255)
                                                                                                                       attattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAAtt                                        <  3:648165/139‑1 (MQ=255)
                                                                                                                       attattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAAtt                                        >  4:648165/1‑139 (MQ=255)
                                                                                                                               gCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    >  1:1078334/1‑135 (MQ=255)
                                                                                                                                 gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                    >  2:696416/1‑149 (MQ=255)
                                                                                                                                  aTAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGt                                                    >  4:95409/1‑116 (MQ=255)
                                                                                                                                    aaaaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTc                                                                                 <  4:1078435/85‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCa                >  2:525937/1‑149 (MQ=255)
                                                                                                                                      aaataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCAc               >  3:1419022/1‑149 (MQ=255)
                                                                                                                                      aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCAc               >  4:29513/1‑149 (MQ=255)
                                                                                                                                       aataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACt              >  1:1293328/4‑149 (MQ=255)
                                                                                                                                       aaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtctc                                                                     <  4:1024493/94‑1 (MQ=255)
                                                                                                                                       aaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtctc                                                                     >  3:1024493/1‑94 (MQ=255)
                                                                                                                                       aaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACt              <  2:1534572/149‑1 (MQ=255)
                                                                                                                                        ataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACtt             >  1:476641/3‑149 (MQ=255)
                                                                                                                                         aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    >  2:919629/1‑125 (MQ=255)
                                                                                                                                         aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    >  3:1096323/1‑125 (MQ=255)
                                                                                                                                         aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACttt            >  4:367335/1‑149 (MQ=255)
                                                                                                                                          aGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTa           >  4:248251/1‑149 (MQ=255)
                                                                                                                                            ccTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATc         <  1:1560634/149‑1 (MQ=255)
                                                                                                                                               gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      >  2:1306156/1‑149 (MQ=255)
                                                                                                                                               gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      <  3:160506/149‑1 (MQ=255)
                                                                                                                                               gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      <  1:1025274/149‑1 (MQ=255)
                                                                                                                                                  aGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACtt             >  4:793908/1‑139 (MQ=255)
                                                                                                                                                   gATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGaaa  <  2:213949/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAA  >  NC_000913/3722153‑3722448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: