Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,886,756 G→A 7.1% intergenic (+34/‑72) yidC → / → mnmE membrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,886,7560GA 7.1% 589.1 / 22.1 183intergenic (+34/‑72)yidC/mnmEmembrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base G (88/82);  new base A (8/5);  total (96/87)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 189 total aligned reads displayed.

AGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGG  >  NC_000913/3886608‑3886904
                                                                                                                                                    |                                                                                                                                                    
aGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                      <  3:368622/149‑1 (MQ=255)
  gTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                    >  1:666610/1‑149 (MQ=255)
   tCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTg                                                                                                                                                   >  4:90162/1‑149 (MQ=255)
   tCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTg                                                                                                                                                   >  3:173631/1‑149 (MQ=255)
        tGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGc                                                                                                                                              <  4:165044/149‑1 (MQ=255)
         gCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGcc                                                                                                                                             <  4:301954/149‑1 (MQ=255)
          cTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                            <  4:742442/149‑2 (MQ=255)
            gTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttt                                                                                                                                          <  1:94297/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         <  3:1298507/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         <  3:764714/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         <  1:1343783/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGCCctta                                                                                                                                         >  3:297703/1‑148 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATGCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         <  4:1465194/149‑1 (MQ=255)
                tataTCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttc                                                                                                                                      <  1:52555/149‑1 (MQ=255)
                   atCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttt                                                                                                                                   >  3:708639/1‑149 (MQ=255)
                    tCGTCAGCAACCTGGTAGCCATTATTCAGCAGCAGCCGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctttt                                                                                                                                  <  4:1061664/149‑1 (MQ=255)
                    tCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctttt                                                                                                                                  >  4:204702/1‑149 (MQ=255)
                         aGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAg                                                                                                                             <  2:864423/149‑1 (MQ=255)
                         aGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAg                                                                                                                             >  1:856035/1‑149 (MQ=255)
                          gCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAgg                                                                                                                            >  1:329417/1‑149 (MQ=255)
                             aCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCg                                                                                                                         >  1:1293000/1‑149 (MQ=255)
                              ccTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCgg                                                                                                                        <  4:266483/149‑1 (MQ=255)
                                   tAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAg                                                                                                                   <  2:512412/149‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         >  3:1412294/1‑123 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAg                                                                                                                   <  3:588710/145‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                               >  2:1541572/1‑149 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                               >  2:1316595/1‑149 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                                <  3:479450/148‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                                >  4:479450/1‑148 (MQ=255)
                                         taaatCAGCTGCTGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcgc                                                                                                             >  1:750490/5‑149 (MQ=255)
                                           attCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCgcgc                                                                                                           <  3:90162/149‑1 (MQ=255)
                                            ttCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCa                                                                                                          >  1:1409041/1‑149 (MQ=255)
                                            ttCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCa                                                                                                          >  2:398217/1‑149 (MQ=255)
                                              cagcagcagCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATc                                                                                                        <  4:1144612/149‑1 (MQ=255)
                                                gcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCg                                                                                                      >  1:1308438/1‑149 (MQ=255)
                                                 cagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGc                                                                                                     >  4:1135363/1‑149 (MQ=255)
                                                  agcagcTGATTTACCGGGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCg                                                                                                                         <  2:712343/128‑1 (MQ=255)
                                                     agcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcaca                                                                                                 <  1:1230050/149‑1 (MQ=255)
                                                         gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                             <  3:1076153/149‑1 (MQ=255)
                                                         gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                             <  1:774531/149‑1 (MQ=255)
                                                          aTTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAg                                                                                            <  1:881037/149‑1 (MQ=255)
                                                          aTTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAg                                                                                            >  1:1199351/1‑149 (MQ=255)
                                                           tttACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGc                                                                                           >  2:88231/1‑149 (MQ=255)
                                                             tACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                                         <  3:576066/149‑1 (MQ=255)
                                                              aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAAc                                                                                        <  1:1524445/149‑1 (MQ=255)
                                                                cGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAg                                                                                      >  2:297057/1‑149 (MQ=255)
                                                                 gTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAgg                                                                                     >  3:939816/1‑149 (MQ=255)
                                                                    gTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAc                                                                                  >  4:1313031/1‑149 (MQ=255)
                                                                    gTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAc                                                                                  <  4:389351/149‑1 (MQ=255)
                                                                    gTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATACTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCTTAGGGCGGATAAGCACCACGCATCCGCCACATAAAGCAACAGAAAc                                                                                  >  2:1411377/1‑149 (MQ=255)
                                                                       tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatc                                                                               <  3:447259/149‑1 (MQ=255)
                                                                       tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGACGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatc                                                                               >  1:39563/1‑149 (MQ=255)
                                                                         gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                             <  2:856035/149‑1 (MQ=255)
                                                                         gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                             >  2:1224045/1‑149 (MQ=255)
                                                                         gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                             >  1:1511040/1‑149 (MQ=255)
                                                                         gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                             <  4:666857/149‑1 (MQ=255)
                                                                         gAAAAACGTGGCCTGCATACCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttct                                                                                                                                     >  3:1482682/1‑93 (MQ=255)
                                                                            aaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                          <  4:549331/149‑1 (MQ=255)
                                                                            aaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                          <  4:939816/149‑1 (MQ=255)
                                                                                gTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGc                                                                         >  2:241859/1‑146 (MQ=255)
                                                                                       gCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGaca                                                               <  1:100202/149‑1 (MQ=255)
                                                                                         aTAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                          <  3:611402/136‑1 (MQ=255)
                                                                                           aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttttt                                                                                                                                        >  1:843172/1‑72 (MQ=255)
                                                                                               gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACATTATCGTa                                                       <  2:966174/149‑1 (MQ=255)
                                                                                               gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                       <  3:248556/149‑1 (MQ=255)
                                                                                               gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGACTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGGCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                       <  4:168171/149‑1 (MQ=255)
                                                                                                     agaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGACAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAg                                                 >  3:1004716/1‑149 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         >  3:1382681/1‑60 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                         <  4:1382681/60‑1 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                                >  1:488022/1‑149 (MQ=255)
                                                                                                       aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCTCCACACAAAGCAACa                                                                                       >  1:1178781/1‑109 (MQ=255)
                                                                                                       aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                       <  2:1178781/109‑1 (MQ=255)
                                                                                                            aTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGc                                          <  4:6005/149‑1 (MQ=255)
                                                                                                             tCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGcc                                         >  4:1527128/1‑149 (MQ=255)
                                                                                                                  aTTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                             <  4:222894/108‑1 (MQ=255)
                                                                                                                  aTTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCgg                                    <  3:347030/149‑1 (MQ=255)
                                                                                                                    catgTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGa                                  <  4:1227529/146‑1 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCa                                             >  1:1057156/1‑134 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCa                                             <  2:1057156/134‑1 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTg                              >  4:483322/1‑149 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTg                              >  2:1278336/1‑149 (MQ=255)
                                                                                                                          aGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTggc                            <  2:781964/149‑1 (MQ=255)
                                                                                                                                cGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                      <  2:627395/149‑1 (MQ=255)
                                                                                                                                 gCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                     <  4:249061/149‑1 (MQ=255)
                                                                                                                                     aaaTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCAt                 >  2:1396606/1‑149 (MQ=255)
                                                                                                                                       aTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATcc               <  4:1413228/149‑1 (MQ=255)
                                                                                                                                         aaaggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACATAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                       >  4:161813/4‑107 (MQ=255)
                                                                                                                                         aaaggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                       <  3:161813/104‑1 (MQ=255)
                                                                                                                                         aaGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGa                                  <  4:71378/128‑1 (MQ=255)
                                                                                                                                          aGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgc            <  4:708639/149‑1 (MQ=255)
                                                                                                                                           gggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGt                                                        <  3:1202411/104‑1 (MQ=255)
                                                                                                                                           aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                      >  1:235720/2‑138 (MQ=255)
                                                                                                                                           aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                     >  3:218223/2‑139 (MQ=255)
                                                                                                                                           aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcg           >  1:1570394/2‑149 (MQ=255)
                                                                                                                                            ggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcgc          >  4:432819/1‑149 (MQ=255)
                                                                                                                                              cGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAt        <  3:717611/149‑1 (MQ=255)
                                                                                                                                              cGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAt        <  3:12352/149‑1 (MQ=255)
                                                                                                                                                 tCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAtctc     >  1:964090/1‑149 (MQ=255)
                                                                                                                                                 tCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAtctc     >  2:447768/1‑149 (MQ=255)
                                                                                                                                                    gTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCgg  >  2:1014659/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGG  >  NC_000913/3886608‑3886904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: