Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,886,757 T→C 7.1% intergenic (+35/‑71) yidC → / → mnmE membrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,886,7570TC 7.1% 573.6 / 24.4 182intergenic (+35/‑71)yidC/mnmEmembrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base T (87/82);  new base C (8/5);  total (95/87)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 187 total aligned reads displayed.

GTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGC  >  NC_000913/3886610‑3886905
                                                                                                                                                   |                                                                                                                                                     
gTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                      >  1:666610/1‑149 (MQ=255)
 tCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTg                                                                                                                                                     >  4:90162/1‑149 (MQ=255)
 tCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTg                                                                                                                                                     >  3:173631/1‑149 (MQ=255)
      tGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGc                                                                                                                                               <  4:165044/149‑1 (MQ=255)
       gCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGcc                                                                                                                                              <  4:301954/149‑1 (MQ=255)
        cTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                             <  4:742442/149‑2 (MQ=255)
          gTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttt                                                                                                                                           >  3:1061664/1‑149 (MQ=255)
          gTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttt                                                                                                                                           <  1:94297/149‑1 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  1:1343783/149‑1 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  3:1298507/149‑1 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  3:764714/149‑1 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGCCctta                                                                                                                                          >  3:297703/1‑148 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATGCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  4:1465194/149‑1 (MQ=255)
            aCTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGCCcttat                                                                                                                                         <  2:285336/149‑3 (MQ=255)
             cTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttttt                                                                                                                                        <  3:912766/149‑1 (MQ=255)
                 atCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttt                                                                                                                                    <  3:568343/149‑1 (MQ=255)
                 atCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttt                                                                                                                                    >  3:708639/1‑149 (MQ=255)
                  tCGTCAGCAACCTGGTAGCCATTATTCAGCAGCAGCCGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctttt                                                                                                                                   <  4:1061664/149‑1 (MQ=255)
                    gTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCg                                                                                                                                 <  4:192708/149‑1 (MQ=255)
                        gCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAgg                                                                                                                             >  1:329417/1‑149 (MQ=255)
                           aCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCg                                                                                                                          >  1:1293000/1‑149 (MQ=255)
                            ccTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCgg                                                                                                                         <  4:266483/149‑1 (MQ=255)
                                 tAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAg                                                                                                                    <  2:512412/149‑1 (MQ=255)
                                   aCCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCa                                                                                                                  <  2:1043053/149‑1 (MQ=255)
                                    ccATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                                 <  2:332507/149‑1 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          >  3:1412294/1‑123 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  4:1412294/123‑1 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAg                                                                                                                    >  4:588710/1‑145 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAg                                                                                                                    <  3:588710/145‑1 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                                >  2:1316595/1‑149 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                                >  1:781964/1‑149 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                                 <  3:479450/148‑1 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                                 >  4:479450/1‑148 (MQ=255)
                                       taaatCAGCTGCTGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcgc                                                                                                              >  1:750490/5‑149 (MQ=255)
                                             agcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATcc                                                                                                        <  1:1395432/149‑1 (MQ=255)
                                              gcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCg                                                                                                       >  1:1308438/1‑149 (MQ=255)
                                               cagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGc                                                                                                      >  4:1135363/1‑149 (MQ=255)
                                                   agcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcaca                                                                                                  <  1:1230050/149‑1 (MQ=255)
                                                    gcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcacac                                                                                                 >  2:774531/1‑149 (MQ=255)
                                                    gcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcacac                                                                                                 <  1:1431520/149‑1 (MQ=255)
                                                    gcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcacac                                                                                                 >  4:248556/1‑149 (MQ=255)
                                                       gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                              <  3:1076153/149‑1 (MQ=255)
                                                        aTTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAg                                                                                             >  1:1199351/1‑149 (MQ=255)
                                                          ttACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCa                                                                                           >  2:1524445/1‑149 (MQ=255)
                                                           tACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                                          <  4:1359080/149‑1 (MQ=255)
                                                           tACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                                          <  4:520282/149‑1 (MQ=255)
                                                            aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAAc                                                                                         <  1:1524445/149‑1 (MQ=255)
                                                               gTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAgg                                                                                      >  3:939816/1‑149 (MQ=255)
                                                                tGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGa                                                                                     <  2:1409041/149‑1 (MQ=255)
                                                                  gTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAc                                                                                   <  4:389351/149‑1 (MQ=255)
                                                                   tCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                              >  3:350301/1‑137 (MQ=255)
                                                                     tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatc                                                                                <  3:447259/149‑1 (MQ=255)
                                                                     tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGACGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatc                                                                                >  1:39563/1‑149 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              >  3:1547404/1‑149 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              >  1:505818/1‑149 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              >  2:1224045/1‑149 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              <  4:666857/149‑1 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATACCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttct                                                                                                                                      >  3:1482682/1‑93 (MQ=255)
                                                                          aaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                           <  4:939816/149‑1 (MQ=255)
                                                                                   cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                                  >  2:873109/1‑149 (MQ=255)
                                                                                   cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                                  <  1:88231/149‑1 (MQ=255)
                                                                                   cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                                  >  2:1070636/1‑149 (MQ=255)
                                                                                    tGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGac                                                                 >  1:966174/1‑149 (MQ=255)
                                                                                     gCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGaca                                                                <  1:100202/149‑1 (MQ=255)
                                                                                       aTAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                           <  3:611402/136‑1 (MQ=255)
                                                                                         aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttttt                                                                                                                                         <  2:843172/72‑1 (MQ=255)
                                                                                             gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACATTATCGTa                                                        <  2:966174/149‑1 (MQ=255)
                                                                                             gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                        <  3:248556/149‑1 (MQ=255)
                                                                                             gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                        >  4:1234434/1‑149 (MQ=255)
                                                                                                    gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          >  3:1382681/1‑60 (MQ=255)
                                                                                                    gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                                 >  1:488022/1‑149 (MQ=255)
                                                                                                    gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                                 >  2:672162/1‑149 (MQ=255)
                                                                                                     aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCTCCACACAAAGCAACa                                                                                        >  1:1178781/1‑109 (MQ=255)
                                                                                                     aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGc                                                >  1:843341/1‑149 (MQ=255)
                                                                                                      aaaaaTCCTGATTCGGTGAGTTTTCGTTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGcc                                               >  2:1378687/1‑149 (MQ=255)
                                                                                                          aTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGc                                           <  4:6005/149‑1 (MQ=255)
                                                                                                           tCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGcc                                          >  4:1527128/1‑149 (MQ=255)
                                                                                                                      tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCa                                              >  1:1057156/1‑134 (MQ=255)
                                                                                                                      tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTg                               >  2:1278336/1‑149 (MQ=255)
                                                                                                                               gCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                      <  4:249061/149‑1 (MQ=255)
                                                                                                                                 tAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGc                    <  2:566908/149‑1 (MQ=255)
                                                                                                                                   aaaGAAGGGCGGTCAGTTGACCGCCTATTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCAt                  >  2:863108/1‑149 (MQ=255)
                                                                                                                                    aaTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAg                                                                                       >  3:897553/1‑79 (MQ=255)
                                                                                                                                     aTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATcc                <  4:1413228/149‑1 (MQ=255)
                                                                                                                                      aaaaggCGGTCAACTGACCGCCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATcc                >  1:868460/5‑149 (MQ=255)
                                                                                                                                       aaaggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACATAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                        >  4:161813/4‑107 (MQ=255)
                                                                                                                                       aaGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGa                                   <  4:71378/128‑1 (MQ=255)
                                                                                                                                        aGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgc             <  4:708639/149‑1 (MQ=255)
                                                                                                                                         gggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGt                                                         >  4:1202411/1‑104 (MQ=255)
                                                                                                                                         gggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGt                                                         <  3:1202411/104‑1 (MQ=255)
                                                                                                                                         aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                       <  2:235720/137‑1 (MQ=255)
                                                                                                                                         aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                       >  1:235720/2‑138 (MQ=255)
                                                                                                                                         aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                      <  4:218223/138‑1 (MQ=255)
                                                                                                                                         aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                      >  3:218223/2‑139 (MQ=255)
                                                                                                                                         aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcg            >  1:1570394/2‑149 (MQ=255)
                                                                                                                                          ggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcgc           >  4:432819/1‑149 (MQ=255)
                                                                                                                                            cGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAt         <  3:717611/149‑1 (MQ=255)
                                                                                                                                            cGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAt         <  3:12352/149‑1 (MQ=255)
                                                                                                                                               tCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAtctc      >  2:447768/1‑149 (MQ=255)
                                                                                                                                                   ttGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGc  >  1:571010/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGC  >  NC_000913/3886610‑3886905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: