Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,008,416 T→A 5.8% intergenic (+39/+23) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4160TA 5.8% 597.4 / 20.6 172intergenic (+39/+23)rhtC/rhtBthreonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
Reads supporting (aligned to +/- strand):  ref base T (86/76);  new base A (3/7);  total (89/83)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 177 total aligned reads displayed.

CCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGC  >  NC_000913/4008272‑4008564
                                                                                                                                                |                                                                                                                                                    
     cGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttat                                                                                                                                             <  1:523785/149‑5 (MQ=255)
      gCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttatc                                                                                                                                            <  1:959125/149‑6 (MQ=255)
         aaTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGa                                                                                                                                         <  2:748646/149‑1 (MQ=255)
          aTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                        <  1:925847/149‑1 (MQ=255)
               ccGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttat                                                                                                                                             <  1:104764/139‑5 (MQ=255)
               ccGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttat                                                                                                                                             >  2:104764/1‑135 (MQ=255)
               ccGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACattat                                                                                                                                   >  3:1056996/1‑149 (MQ=255)
                  tGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTAtttt                                                                                                                                <  2:1394471/149‑1 (MQ=255)
                    gTTATCAACGTCTGGCGAAGTGTATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTTGCATTCATTTGATTATTTCGCGGTTATGCCAGACGCATCTTCAGAGTAAATCGGATAAGGCGTTTACGCCGCACCCGACTtttttttta                                                                                                                              <  4:540675/149‑2 (MQ=255)
                      tATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCAc                                                                                                                            >  4:763790/1‑149 (MQ=255)
                        tCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGc                                                                                                                          >  1:1241572/1‑149 (MQ=255)
                            cGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGc                                                                                                                      <  4:1204267/149‑1 (MQ=255)
                             gTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGcc                                                                                                                     >  4:1346438/1‑149 (MQ=255)
                             gTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGcc                                                                                                                     >  1:1310133/1‑149 (MQ=255)
                                tGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCg                                                                                                                  >  4:155967/1‑149 (MQ=255)
                                  gCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGcc                                                                                                                >  3:552803/1‑149 (MQ=255)
                                  gCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGcc                                                                                                                <  4:921863/149‑1 (MQ=255)
                                    gAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGa                                                                                                              <  2:807367/149‑1 (MQ=255)
                                      aGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATg                                                                                                            <  2:299671/149‑1 (MQ=255)
                                         ggATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTa                                                                                                         >  1:562159/1‑149 (MQ=255)
                                         ggATTGATGGTTTTGCCGGGGAGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGATa                                                                                                         >  4:792396/1‑149 (MQ=255)
                                          gATTGATGGTTTTGCCGGGGTGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTaa                                                                                                        >  2:346606/1‑149 (MQ=255)
                                           aTTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAAc                                                                                                       >  4:828425/1‑149 (MQ=255)
                                           aTTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAAc                                                                                                       >  3:324546/1‑149 (MQ=255)
                                            ttGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACa                                                                                                      >  1:280212/1‑149 (MQ=255)
                                            ttGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACa                                                                                                      >  3:1436968/1‑149 (MQ=255)
                                            ttGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACa                                                                                                      >  3:852834/1‑149 (MQ=255)
                                            ttGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATGCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACa                                                                                                      >  2:17887/1‑149 (MQ=255)
                                              gATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgc                                                                                                    >  3:772301/1‑149 (MQ=255)
                                               aTGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgcg                                                                                                   >  4:1259351/1‑149 (MQ=255)
                                               aTGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgcg                                                                                                   >  1:725321/1‑149 (MQ=255)
                                               aTGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgcg                                                                                                   >  4:1348723/1‑149 (MQ=255)
                                                tGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgcgc                                                                                                  <  3:1206498/149‑1 (MQ=255)
                                                 ggTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCt                                                                                                 <  1:879634/149‑1 (MQ=255)
                                                 ggTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCt                                                                                                 <  2:1168527/149‑1 (MQ=255)
                                                  gTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGAGGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttatc                                                                                                                                            >  2:1255138/1‑100 (MQ=255)
                                                   ttttGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTcc                                                                                               <  3:1348723/149‑1 (MQ=255)
                                                      tGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCccac                                                                                            <  3:1516355/149‑1 (MQ=255)
                                                        ccGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCccacca                                                                                          <  3:1241379/149‑1 (MQ=255)
                                                               gTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATaaa                                                                                   >  4:1109193/1‑149 (MQ=255)
                                                                ttATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaac                                                                                  >  1:1511202/1‑149 (MQ=255)
                                                                   tttGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaac                                                                               <  4:1165773/149‑1 (MQ=255)
                                                                   tttGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaac                                                                               <  3:1259351/149‑1 (MQ=255)
                                                                   tttGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaac                                                                               <  3:386406/149‑1 (MQ=255)
                                                                    ttGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACg                                                                              <  4:1436968/149‑1 (MQ=255)
                                                                     tGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGa                                                                             <  3:821147/149‑1 (MQ=255)
                                                                     tGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGa                                                                             <  4:250625/149‑1 (MQ=255)
                                                                     tGCCGGATATGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGa                                                                             <  2:1305534/149‑1 (MQ=255)
                                                                       ccGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCAt                                                                                                                        <  2:74697/104‑1 (MQ=255)
                                                                            tttGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGaaa                                                                      >  4:778434/1‑149 (MQ=255)
                                                                                 cattcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCtt                                                                 <  1:1185650/149‑1 (MQ=255)
                                                                                  attcattTGCTTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTCAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                                >  4:453244/1‑149 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGc                                                                                                           <  3:1519833/106‑1 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGc                                                                                                           >  4:1519833/1‑106 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGa                                                                             >  2:142879/1‑136 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                                >  1:1153751/1‑149 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                                >  2:1382534/1‑149 (MQ=255)
                                                                                  attcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                                >  3:243404/1‑149 (MQ=255)
                                                                                   ttcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTAt                                                               >  1:763351/1‑149 (MQ=255)
                                                                                   ttcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTAt                                                               >  1:375540/1‑149 (MQ=255)
                                                                                       tttGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                           >  1:1429203/1‑149 (MQ=255)
                                                                                       tttGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACCCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                           >  2:24432/1‑149 (MQ=255)
                                                                                          gATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAgcgc                                                        <  3:1348969/149‑1 (MQ=255)
                                                                                            ttattTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCt                                                      >  1:967098/1‑149 (MQ=255)
                                                                                            ttattTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCt                                                      >  3:165677/1‑149 (MQ=255)
                                                                                              attTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTc                                                    <  3:47466/149‑1 (MQ=255)
                                                                                                  cgcgGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTaa                                                                                                        <  3:1419810/93‑1 (MQ=255)
                                                                                                      gTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCtt                                            <  4:324546/149‑1 (MQ=255)
                                                                                                           gCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTaa                                                                                                        <  1:1015347/84‑1 (MQ=255)
                                                                                                              aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCtt                                    <  4:1518874/149‑1 (MQ=255)
                                                                                                              aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCtt                                    <  2:1161191/149‑1 (MQ=255)
                                                                                                              aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCtt                                    >  1:1478808/1‑149 (MQ=255)
                                                                                                              aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCtt                                    <  4:53715/149‑1 (MQ=255)
                                                                                                                aCGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTa                                  <  3:258343/149‑1 (MQ=255)
                                                                                                                  gcgTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaac                                                                               <  3:1517158/102‑1 (MQ=255)
                                                                                                                  gcgTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAAt                                <  2:562159/149‑1 (MQ=255)
                                                                                                                  gcgTCTTCAGAGTAAGTCGGATAAGCCGTTTACGACGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGGCCTTTAAt                                <  2:967384/149‑1 (MQ=255)
                                                                                                                   cgTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCCGCGCCTTCATCTGCTTTGGTCCTTTAATc                               <  3:1346438/149‑1 (MQ=255)
                                                                                                                       ttCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACTAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATa                           >  3:1138894/1‑149 (MQ=255)
                                                                                                                       ttCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACACCGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATa                           <  2:1445828/149‑1 (MQ=255)
                                                                                                                       ttCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATa                           >  2:1229770/1‑149 (MQ=255)
                                                                                                                         cAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATaga                         <  3:255091/149‑1 (MQ=255)
                                                                                                                           gagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGc                       >  4:1390951/1‑149 (MQ=255)
                                                                                                                             gTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCCTTAATCCATAGAGCaa                     >  2:896747/1‑149 (MQ=255)
                                                                                                                               aaGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATc                   <  2:379154/149‑1 (MQ=255)
                                                                                                                                 gTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCg                 >  1:671125/1‑149 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGtt               <  1:1392591/149‑1 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGtt               <  4:629201/149‑1 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGtt               <  1:1425287/149‑1 (MQ=255)
                                                                                                                                     gacaaGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATc                               <  3:1289558/128‑1 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGc                                                                           >  2:697604/1‑87 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                                <  1:1271123/98‑1 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATc                               >  4:1289558/1‑131 (MQ=255)
                                                                                                                                        aaGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCtt                                    <  3:962259/123‑1 (MQ=255)
                                                                                                                                        aaGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCa          >  3:671313/1‑149 (MQ=255)
                                                                                                                                         aGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCGCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCaa         <  1:1137943/149‑1 (MQ=255)
                                                                                                                                          ggCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAg        <  2:671125/149‑1 (MQ=255)
                                                                                                                                           gCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTg                                          >  3:78034/1‑114 (MQ=255)
                                                                                                                                             gTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGt     <  2:967098/149‑1 (MQ=255)
                                                                                                                                             gTTTACCCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGt     <  1:24432/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGC  >  NC_000913/4008272‑4008564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: