Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,110,717 A→T 6.7% intergenic (+32/+23) cdh → / ← tpiA CDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,110,7170AT 6.7% 568.2 / 21.7 163intergenic (+32/+23)cdh/tpiACDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (82/70);  new base T (8/3);  total (90/73)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

ATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAC  >  NC_000913/4110569‑4110863
                                                                                                                                                    |                                                                                                                                                  
aTGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGata                                                                                                                                                    <  2:1057078/149‑1 (MQ=255)
      cGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAAt                                                                                                                                              >  4:660527/1‑149 (MQ=255)
        gCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATcc                                                                                                                                            <  3:660527/149‑1 (MQ=255)
        gCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATcc                                                                                                                                            >  2:1015673/1‑149 (MQ=255)
         cAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCg                                                                                                                                           <  2:1267580/149‑1 (MQ=255)
          agagTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCgg                                                                                                                                          <  3:48923/149‑1 (MQ=255)
           gagTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGc                                                                                                                                         <  1:1035740/149‑1 (MQ=255)
              tGATAACTCATTTATATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCAcc                                                                                                                                      >  2:850783/1‑149 (MQ=255)
                   aCTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCa                                                                                                                                 <  4:517213/149‑1 (MQ=255)
                    cTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAg                                                                                                                                >  4:1438718/1‑149 (MQ=255)
                     tCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGa                                                                                                                               >  4:1150183/1‑149 (MQ=255)
                       aTTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACt                                                                                                                             >  1:459847/1‑149 (MQ=255)
                        tttGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATCCGGCActtac                                                                                                                                   <  2:560774/142‑5 (MQ=255)
                        tttGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATCCGGCActtac                                                                                                                                   >  1:560774/1‑138 (MQ=255)
                        tttGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACtt                                                                                                                            >  4:861869/1‑149 (MQ=255)
                            tATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGc                                                                                                                        <  4:537484/149‑1 (MQ=255)
                              ttGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCt                                                                                                                      >  4:1148560/1‑149 (MQ=255)
                                 cTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGtt                                                                                                                   <  3:1433758/149‑1 (MQ=255)
                                   ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTa                                                                                                                 >  1:1408578/1‑149 (MQ=255)
                                   ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTa                                                                                                                 <  4:149523/149‑1 (MQ=255)
                                   ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTa                                                                                                                 <  1:1424564/149‑1 (MQ=255)
                                    gCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAg                                                                                                                <  3:777517/149‑1 (MQ=255)
                                    gCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAg                                                                                                                >  3:1412746/1‑149 (MQ=255)
                                         acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCATGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCt                                                                                                                                     >  3:550217/1‑123 (MQ=255)
                                         acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCATGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCt                                                                                                                                     <  4:550217/123‑1 (MQ=255)
                                         acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCGGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCt                                                                                                           >  4:1166798/1‑149 (MQ=255)
                                           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTc                                                                                                         <  4:182203/149‑1 (MQ=255)
                                           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTc                                                                                                         >  2:666833/1‑149 (MQ=255)
                                           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTc                                                                                                         >  3:407831/1‑149 (MQ=255)
                                           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTc                                                                                                         >  3:667957/1‑149 (MQ=255)
                                            aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCt                                                                                                        >  4:1354453/1‑149 (MQ=255)
                                                 aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGc                                                                                                   <  1:170864/149‑1 (MQ=255)
                                                    tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGtt                                                                                                                   <  1:55782/130‑1 (MQ=255)
                                                    tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCttt                                                                                                >  4:1013121/1‑149 (MQ=255)
                                                      cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGc                                                                                                                        >  3:1421064/1‑123 (MQ=255)
                                                       tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTaa                                                                                              <  3:1192875/148‑1 (MQ=255)
                                                       tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                             >  3:1102522/1‑149 (MQ=255)
                                                         aCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGa                                                                                           >  2:1110491/1‑149 (MQ=255)
                                                           gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTaa                                                                                              >  1:715037/1‑144 (MQ=255)
                                                           gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGAtt                                                                                         <  4:418726/149‑1 (MQ=255)
                                                             ttAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTAc                                                                                       >  1:897051/1‑149 (MQ=255)
                                                               taCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATg                                                                                                                                                  <  3:978419/87‑1 (MQ=255)
                                                               aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGa                                                                                                                                                 <  2:1479128/89‑1 (MQ=255)
                                                               aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGa                                                                                                                                                 >  1:1479128/1‑89 (MQ=255)
                                                                aCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGc                                                                                    <  3:179442/149‑1 (MQ=255)
                                                                    tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTAAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                             >  3:1079503/1‑136 (MQ=255)
                                                                    tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTAAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                             <  4:1079503/136‑1 (MQ=255)
                                                                      cTTCAGCCGAAGAAAATCAGGAATCTCAGTGTGAGAATTTGCCGTAAGGGGGAGAGTTAAGGAAAGTAAGTGCCGGAAAAGAAATCCGGCAACTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGc                                                                              <  1:454862/149‑1 (MQ=255)
                                                                       ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCg                                                                             >  3:1168290/1‑149 (MQ=255)
                                                                       ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCg                                                                             >  2:826654/1‑149 (MQ=255)
                                                                       ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCg                                                                             >  4:1457423/1‑149 (MQ=255)
                                                                       ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTAATGCGaa                                                                                 >  3:997937/1‑145 (MQ=255)
                                                                         cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATAGGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACGGCGAAGGCGTc                                                                           >  2:1069906/1‑149 (MQ=255)
                                                                          aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCa                                                                          >  1:1424454/1‑149 (MQ=255)
                                                                          aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCa                                                                          <  4:1232734/149‑1 (MQ=255)
                                                                            ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGaa                                                                                 <  2:1525448/140‑1 (MQ=255)
                                                                            ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGaa                                                                                 >  1:1525448/1‑140 (MQ=255)
                                                                              gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCtt                                                                      <  1:1116909/149‑1 (MQ=255)
                                                                                 gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCa                                                                   <  2:97504/149‑1 (MQ=255)
                                                                                 gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCa                                                                   <  2:1424454/149‑1 (MQ=255)
                                                                                      tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGaa                                                              <  1:1069906/149‑1 (MQ=255)
                                                                                      tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGaa                                                              >  1:850010/1‑149 (MQ=255)
                                                                                       cAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAg                                                             <  1:1345600/149‑1 (MQ=255)
                                                                                        aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTc                                                                    >  1:533938/1‑141 (MQ=255)
                                                                                        aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTc                                                                    <  2:533938/141‑1 (MQ=255)
                                                                                        aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGc                                                            <  2:544120/149‑1 (MQ=255)
                                                                                          gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGctc                                                          >  4:824899/1‑147 (MQ=255)
                                                                                                 aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCaccaac                                                   <  4:1412746/149‑1 (MQ=255)
                                                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACtt                                                                                                                            >  4:126383/1‑72 (MQ=255)
                                                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACtt                                                                                                                            <  3:126383/72‑1 (MQ=255)
                                                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgc                                               >  1:633837/1‑149 (MQ=255)
                                                                                                       agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcgc                                             <  3:1013121/149‑1 (MQ=255)
                                                                                                          ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGcc                                          >  2:524703/1‑149 (MQ=255)
                                                                                                              gCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCg                                      <  3:1461538/149‑1 (MQ=255)
                                                                                                                        gAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCt                            >  3:710394/1‑149 (MQ=255)
                                                                                                                        gAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCt                            <  2:1415797/149‑1 (MQ=255)
                                                                                                                        gAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCt                            <  1:1015673/149‑1 (MQ=255)
                                                                                                                          agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGa                          <  4:676689/149‑1 (MQ=255)
                                                                                                                          agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGa                          <  3:1148560/149‑1 (MQ=255)
                                                                                                                              ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCaa                      >  1:200901/1‑149 (MQ=255)
                                                                                                                              ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCaa                      >  1:89410/1‑149 (MQ=255)
                                                                                                                               tAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCCGCGCGCCGTCGATATCCGGCTGAGCaaa                     <  1:396198/149‑1 (MQ=255)
                                                                                                                                  ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGat                                    <  2:724547/131‑1 (MQ=255)
                                                                                                                                  ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGat                                    >  1:724547/1‑131 (MQ=255)
                                                                                                                                   gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGt                 >  1:372371/1‑149 (MQ=255)
                                                                                                                                     aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGaa                                                                                 >  1:1172598/1‑83 (MQ=255)
                                                                                                                                     aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGaa                                                                                 <  2:1172598/83‑1 (MQ=255)
                                                                                                                                     aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTc               >  2:179869/1‑149 (MQ=255)
                                                                                                                                     aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTc               <  3:861869/149‑1 (MQ=255)
                                                                                                                                      agtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCt              <  4:799957/149‑1 (MQ=255)
                                                                                                                                       gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATGTCCGGCTGAGCAAACAGTTCTg             >  1:1366536/1‑149 (MQ=255)
                                                                                                                                          ggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAg          >  2:800061/2‑149 (MQ=255)
                                                                                                                                           gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGaa                                                              >  2:1388347/1‑96 (MQ=255)
                                                                                                                                            tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCttt                                                                     <  4:1197504/88‑1 (MQ=255)
                                                                                                                                            tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg        >  4:1312/1‑149 (MQ=255)
                                                                                                                                            tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg        >  2:237392/1‑149 (MQ=255)
                                                                                                                                            tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg        >  3:1439434/1‑149 (MQ=255)
                                                                                                                                             gCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGt       >  1:335747/1‑149 (MQ=255)
                                                                                                                                                 gATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGa   >  4:578929/1‑149 (MQ=255)
                                                                                                                                                  atatGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAc  <  4:994293/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAC  >  NC_000913/4110569‑4110863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: