BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,116,536	G→T	43.4%	N229K (AAC→AAA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,536	0	G	T	43.4%	162.2 / 304.4	213	N229K (AAC→AAA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (51/69); new base T (44/48); total (96/117)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 213 total aligned reads displayed.

CTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACA  >  NC_000913/4116389‑4116683

  cGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCTGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTa                                                                                                                                                  <  4:1074393/149‑1 (MQ=255)

   gCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAg                                                                                                                                                 >  2:1248053/1‑149 (MQ=255)

   gCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAg                                                                                                                                                 >  4:1386092/1‑149 (MQ=255)

        tgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGa                                                                                                                                            <  3:1160988/149‑1 (MQ=255)

        tgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGa                                                                                                                                            <  4:469233/149‑1 (MQ=255)

        tgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGa                                                                                                                                            <  3:633953/149‑1 (MQ=255)

        tgtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTCCACTCACAACTGACCAAACAGCTCTGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGGCTTTGCCGCCAATTTTAGTCTGa                                                                                                                                            >  1:1295069/1‑149 (MQ=255)

         gtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGAc                                                                                                                                           <  3:560658/149‑1 (MQ=255)

          ttCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc                                                                                                                                          >  1:248880/1‑149 (MQ=255)

          ttCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc                                                                                                                                          >  2:747434/1‑149 (MQ=255)

             atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGta                                                                                                                                       >  1:632481/1‑149 (MQ=255)

             atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGta                                                                                                                                       >  3:532346/1‑149 (MQ=255)

                 gCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACt                                                                                                                                   >  1:347641/1‑149 (MQ=255)

                   aTAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTc                                                                                                                                 >  3:57219/1‑149 (MQ=255)

                   aTAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTc                                                                                                                                 >  1:92135/1‑149 (MQ=255)

                   aTAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGGTAGTCTGACCGTATACTTc                                                                                                                                 >  1:1416676/1‑149 (MQ=255)

                      aaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGga                                                                                                                              <  3:1386092/149‑1 (MQ=255)

                       agcagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGCATACTTCGgaa                                                                                                                             <  3:1014931/149‑1 (MQ=255)

                        gcagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaag                                                                                                                            >  1:617255/1‑149 (MQ=255)

                          agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGgaagaa                                                                                                                          <  2:1508589/149‑1 (MQ=255)

                          agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGgaagaa                                                                                                                          <  3:654377/149‑1 (MQ=255)

                          agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGgaagaa                                                                                                                          <  3:194358/149‑1 (MQ=255)

                           gcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGt                                                                                                                                                    <  4:1508084/122‑1 (MQ=255)

                              aGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgac                                                                                                                      <  1:1145984/149‑1 (MQ=255)

                              aGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgac                                                                                                                      <  2:617255/149‑1 (MQ=255)

                               gTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                     >  1:1103465/1‑149 (MQ=255)

                                   cATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt                                                                                                                 >  2:445824/1‑149 (MQ=255)

                                    aTAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACtt                                                                                                                >  2:925431/1‑149 (MQ=255)

                                       ggTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTg                                                                                                             <  4:1253282/149‑1 (MQ=255)

                                        gtgtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTgg                                                                                                            >  2:1097995/1‑149 (MQ=255)

                                        gtgtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGgaa                                                                                                                             >  1:1076071/1‑132 (MQ=255)

                                        gtgtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGgaa                                                                                                                             <  2:1076071/132‑1 (MQ=255)

                                              ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                      <  2:1281335/149‑1 (MQ=255)

                                              ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                      >  4:954791/1‑149 (MQ=255)

                                              ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                      >  2:956371/1‑149 (MQ=255)

                                              ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                      <  1:1507021/149‑1 (MQ=255)

                                              ttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                      >  1:1430337/1‑149 (MQ=255)

                                                cGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtct                                                                                                    >  3:1530273/1‑149 (MQ=255)

                                                     tCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtc                                                                                                     >  2:394291/1‑143 (MQ=255)

                                                      cccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgcgcg                                                                                              >  2:1446685/1‑149 (MQ=255)

                                                       ccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCgg                                                                                             >  3:1475842/1‑149 (MQ=255)

                                                       ccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCgg                                                                                             >  3:95351/1‑149 (MQ=255)

                                                         ttcttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGTGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCTCCAATTTTTGTCTGACCTTATACTTCGGAAGAACGACGCACTTCTGGTAGCATCTCGCGCGGaa                                                                                           >  4:1277452/1‑149 (MQ=255)

                                                              tCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATcc                                                                                      <  1:903022/149‑1 (MQ=255)

                                                              tCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATcc                                                                                      <  3:1093340/149‑1 (MQ=255)

                                                               cACGCACAACTGACCAAACAGCGCGGCCTGATGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCAGTTCTGGCAGCATCTCGCGCGGAATATCCa                                                                                     <  3:1277452/149‑1 (MQ=255)

                                                                aCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAg                                                                                    >  2:134028/1‑149 (MQ=255)

                                                                 cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGc                                                                                   >  1:1269861/1‑149 (MQ=255)

                                                                   cacaACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCAc                                                                                 >  2:1587932/1‑149 (MQ=255)

                                                                    acaACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAg                                                                                    <  1:974426/145‑1 (MQ=255)

                                                                      aaCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTc                                                                              >  1:754784/1‑149 (MQ=255)

                                                                        cggACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGAATCTCGCGCGGAATATCCAGAACTTCCa                                                                            <  4:372965/147‑1 (MQ=255)

                                                                        cTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCa                                                                            <  2:1121860/149‑1 (MQ=255)

                                                                            ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAt                                                                        >  2:556071/1‑149 (MQ=255)

                                                                             cAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                                                       <  1:1472327/149‑1 (MQ=255)

                                                                              aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTCCCAGCAttt                                                                      >  3:1192416/1‑149 (MQ=255)

                                                                              aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGTCGCCTTTGCCGCCAATTTTCGTCTGCACGTATACTTCGGGAGAACGACGCCCTTCTGGCAGCATCTCGCGCAGAATATCACGCACTTCCAGCAttt                                                                      <  4:753190/149‑1 (MQ=255)

                                                                               aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                                                     <  2:248880/149‑1 (MQ=255)

                                                                               aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                                                     <  4:941110/149‑1 (MQ=255)

                                                                               aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                                                     <  3:969405/149‑1 (MQ=255)

                                                                               aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                                                     <  1:1180169/149‑1 (MQ=255)

                                                                                aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaa                                                                                                                             >  2:693379/1‑92 (MQ=255)

                                                                                aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaa                                                                                                                             <  1:693379/92‑1 (MQ=255)

                                                                                aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                                                    <  1:1339543/149‑1 (MQ=255)

                                                                                aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                                                    <  3:346243/149‑1 (MQ=255)

                                                                                aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGTCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                                                    <  1:404344/149‑1 (MQ=255)

                                                                                  aGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                                                  <  3:1134534/149‑1 (MQ=255)

                                                                                  aGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                                                  <  2:669490/149‑1 (MQ=255)

                                                                                       ggCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                                             <  4:1530273/149‑1 (MQ=255)

                                                                                       ggCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                                             <  3:1494157/149‑1 (MQ=255)

                                                                                        gCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCa                                                            <  2:1430337/149‑1 (MQ=255)

                                                                                           tgctgGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTATGACCGTATACTTCGGAAGAacga                                                                                                                       <  3:993818/87‑1 (MQ=255)

                                                                                              tgGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAg                                                      <  3:784469/149‑1 (MQ=255)

                                                                                               ggTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAgg                                                     <  1:383768/149‑1 (MQ=255)

                                                                                               ggTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGt                                                                                                                                        <  1:45085/66‑1 (MQ=255)

                                                                                                   aCCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAt                                                 >  2:312947/1‑149 (MQ=255)

                                                                                                   aCCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAt                                                 >  3:1570224/1‑149 (MQ=255)

                                                                                                     cGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATgg                                               >  1:158166/1‑149 (MQ=255)

                                                                                                       gCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGAt                                             <  3:888157/149‑1 (MQ=255)

                                                                                                             ccGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa                                       <  1:1232068/149‑1 (MQ=255)

                                                                                                             ccGGAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa                                       <  4:1158897/149‑1 (MQ=255)

                                                                                                               ggAGATTGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaaca                                     >  1:783533/1‑149 (MQ=255)

                                                                                                                    ttGGAATACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATg                                <  1:1096206/149‑1 (MQ=255)

                                                                                                                        aaTACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAc                            >  3:469979/1‑149 (MQ=255)

                                                                                                                        aaTACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAc                            <  4:57219/149‑1 (MQ=255)

                                                                                                                          tACGCTTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACga                          >  1:840059/1‑149 (MQ=255)

                                                                                                                          tACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACga                          >  1:168692/1‑149 (MQ=255)

                                                                                                                               gTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGc                     <  4:869641/149‑1 (MQ=255)

                                                                                                                                  ccgccTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGtt                  >  2:829789/1‑149 (MQ=255)

                                                                                                                                   cgccTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTg                 >  1:1275777/1‑149 (MQ=255)

                                                                                                                                     ccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt               <  4:101679/149‑1 (MQ=255)

                                                                                                                                     ccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt               <  4:1123133/149‑1 (MQ=255)

                                                                                                                                     ccTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCCGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGgt               <  2:632481/149‑1 (MQ=255)

                                                                                                                                           ccgccAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATc         <  2:1158478/149‑1 (MQ=255)

                                                                                                                                             gccAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCgg       <  2:654065/149‑1 (MQ=255)

                                                                                                                                             gccAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCgg       >  4:328043/1‑149 (MQ=255)

                                                                                                                                              ccAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTa                             >  1:857460/1‑126 (MQ=255)

                                                                                                                                              ccAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGt      >  4:768647/1‑149 (MQ=255)

                                                                                                                                                aaTTTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTca    <  4:1475842/149‑1 (MQ=255)

                                                                                                                                                aaTGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTca    >  1:286989/1‑149 (MQ=255)

CTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACA  >  NC_000913/4116389‑4116683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`