Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,127,251 C→T 6.3% intergenic (+26/+35) rpmE → / ← yiiX 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,127,2510CT 6.3% 463.1 / 14.6 143intergenic (+26/+35)rpmE/yiiX50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein
Reads supporting (aligned to +/- strand):  ref base C (79/55);  new base T (4/5);  total (83/60)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 146 total aligned reads displayed.

ACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTC  >  NC_000913/4127104‑4127399
                                                                                                                                                   |                                                                                                                                                    
aCCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCa                                                                                                                                                     >  2:77646/1‑149 (MQ=255)
   tGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACgg                                                                                                                                                  >  4:784479/1‑149 (MQ=255)
        ctagACGTCTGCAGCAAGTGCCACCCGTTCTTCAGTGGCAAACAGCGTGATGTTGCTACCGCTGGCCGTGTTGACCGCTTCAACAAGCGTGTCAACATCCCGGGCTGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt                                                                                                                                             <  4:967879/146‑1 (MQ=255)
        cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCtt                                                                                                                                             <  2:920517/149‑1 (MQ=255)
        cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt                                                                                                                                             >  3:1151533/1‑149 (MQ=255)
        cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt                                                                                                                                             >  3:879858/1‑149 (MQ=255)
          cGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCtttt                                                                                                                                           <  4:292809/149‑1 (MQ=255)
               tgtgCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTtgt                                                                                                                                      <  1:184350/149‑1 (MQ=255)
                 tgCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGc                                                                                                                                    <  2:696593/149‑1 (MQ=255)
                    agcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTg                                                                                                                                 >  3:160792/1‑149 (MQ=255)
                      caAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGaa                                                                                                                               <  1:95758/149‑1 (MQ=255)
                           gCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                          <  1:573603/149‑1 (MQ=255)
                           gCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGCTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttttttc                                                                                                                                        >  3:782525/1‑134 (MQ=255)
                           gCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGCTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttttttc                                                                                                                                        <  4:782525/135‑2 (MQ=255)
                             cACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGt                                                                                                                        <  3:101285/149‑1 (MQ=255)
                              aCCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTc                                                                                                                       >  1:1188632/1‑149 (MQ=255)
                              aCCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTc                                                                                                                       <  4:1422421/149‑1 (MQ=255)
                              aCCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGCTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTc                                                                                                                       >  4:707149/1‑149 (MQ=255)
                                 cGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTa                                                                                                                    >  2:1575248/1‑149 (MQ=255)
                                 cGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTa                                                                                                                    <  1:225373/149‑1 (MQ=255)
                                      ttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAcc                                                                                                               >  4:1199666/1‑149 (MQ=255)
                                       tcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              <  1:898383/149‑1 (MQ=255)
                                       tcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              <  3:517411/149‑1 (MQ=255)
                                        cACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCtt                                                                                                                                             <  2:1307561/117‑1 (MQ=255)
                                        cACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCtt                                                                                                                                             >  1:1307561/1‑117 (MQ=255)
                                        cACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCAc                                                                                                             >  4:964449/1‑149 (MQ=255)
                                           tGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaa                                                                                                          >  2:102875/1‑149 (MQ=255)
                                               aaaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACa                                                                                                      >  4:1414745/1‑149 (MQ=255)
                                                aaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAg                                                                                                     <  4:1316191/149‑1 (MQ=255)
                                                  cAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCCCGAAAACAGCg                                                                                                   <  4:152767/149‑1 (MQ=255)
                                                  cAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCg                                                                                                   <  3:1548599/149‑1 (MQ=255)
                                                  cAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCg                                                                                                   <  1:77646/149‑1 (MQ=255)
                                                   aGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCgg                                                                                                  >  1:67242/1‑149 (MQ=255)
                                                            ttGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCttt                                                                                         <  1:1037916/149‑1 (MQ=255)
                                                            ttGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCttt                                                                                         <  1:102875/149‑1 (MQ=255)
                                                                tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  4:735791/1‑149 (MQ=255)
                                                                tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  1:1253401/1‑149 (MQ=255)
                                                                tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  2:1432960/1‑149 (MQ=255)
                                                                tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  1:890152/1‑149 (MQ=255)
                                                                     gTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgt                                                                                >  4:481263/1‑149 (MQ=255)
                                                                       ggCCGTGCTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtgg                                                                              <  3:1350299/149‑1 (MQ=255)
                                                                         ccGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGACAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtggtg                                                                            >  3:325490/1‑149 (MQ=255)
                                                                          cGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGa                                                                           <  3:964449/149‑1 (MQ=255)
                                                                           gtgtTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAg                                                                          >  3:811811/1‑149 (MQ=255)
                                                                             gtTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGtt                                                                        <  3:615202/149‑1 (MQ=255)
                                                                               tGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTtgt                                                                      >  2:235365/1‑149 (MQ=255)
                                                                                gACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCAGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAtttt                                                                                                                           <  1:1201205/95‑1 (MQ=255)
                                                                                 aCCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTgg                                                                    >  2:24147/1‑149 (MQ=255)
                                                                                  ccGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              >  2:366726/1‑106 (MQ=255)
                                                                                  ccGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              <  1:366726/106‑1 (MQ=255)
                                                                                  ccGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATGCTTTGGCGAACGTGGTGAGTTGTGgc                                                                   <  2:1163398/149‑1 (MQ=255)
                                                                                   cGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgcg                                                                  >  3:1235982/1‑149 (MQ=255)
                                                                                      ttCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGc                                                                                      >  1:148389/1‑126 (MQ=255)
                                                                                      ttCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGc                                                                                      <  2:148389/126‑1 (MQ=255)
                                                                                      ttCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCAt                                                               >  1:1257662/1‑149 (MQ=255)
                                                                                          acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCGGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGtt                                                                        >  1:1389025/1‑136 (MQ=255)
                                                                                          acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGtt                                                                        <  2:1389025/136‑1 (MQ=255)
                                                                                            aaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaaaa                                                         >  1:585798/1‑149 (MQ=255)
                                                                                            aaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCGGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaaaa                                                         >  4:1027394/1‑149 (MQ=255)
                                                                                             aGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAAc                                                        >  1:1542685/1‑149 (MQ=255)
                                                                                             aGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAAc                                                        >  2:807967/1‑149 (MQ=255)
                                                                                                  ttCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCtt                                                   >  1:982365/1‑149 (MQ=255)
                                                                                                    cAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTtgt                                                 <  4:435955/149‑1 (MQ=255)
                                                                                                     aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTtgtg                                                                     <  1:407059/128‑1 (MQ=255)
                                                                                                     aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTtgtg                                                                     >  2:407059/1‑128 (MQ=255)
                                                                                                     aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACgg                                                      >  1:702120/1‑143 (MQ=255)
                                                                                                     aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTtgtg                                                >  1:1446122/1‑149 (MQ=255)
                                                                                                      aCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTgg                                               <  2:13325/149‑1 (MQ=255)
                                                                                                         tCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                            >  1:1011351/1‑149 (MQ=255)
                                                                                                         tCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                            >  2:1127226/1‑149 (MQ=255)
                                                                                                         tCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                            <  3:1207426/149‑1 (MQ=255)
                                                                                                           ccGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCgaga                                          <  1:235365/149‑1 (MQ=255)
                                                                                                           ccGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCgaga                                          <  2:649148/149‑1 (MQ=255)
                                                                                                                  gcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGt                                   >  1:1489937/1‑149 (MQ=255)
                                                                                                                   caAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGtt                                  >  3:1370067/1‑149 (MQ=255)
                                                                                                                    aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGCTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGttt                                 >  3:908491/1‑149 (MQ=255)
                                                                                                                     aaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTgg                                                                                       <  3:1448795/94‑1 (MQ=255)
                                                                                                                     aaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                <  1:1368181/149‑1 (MQ=255)
                                                                                                                     aaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                <  2:1011351/149‑1 (MQ=255)
                                                                                                                          gACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCaa                           >  2:1281652/1‑149 (MQ=255)
                                                                                                                            ccGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc                         <  2:1354214/149‑1 (MQ=255)
                                                                                                                               gATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGa                      >  4:587100/1‑149 (MQ=255)
                                                                                                                                aTTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGaa                     <  2:990334/149‑1 (MQ=255)
                                                                                                                                  ttCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAata                   >  3:348902/1‑149 (MQ=255)
                                                                                                                                    ccGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATAtt                 >  4:556351/1‑149 (MQ=255)
                                                                                                                                     cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTc                >  4:216964/1‑149 (MQ=255)
                                                                                                                                     cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTc                >  3:536632/1‑149 (MQ=255)
                                                                                                                                      gAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCt                              >  2:1202859/1‑134 (MQ=255)
                                                                                                                                      gAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAata                   >  2:942835/1‑145 (MQ=255)
                                                                                                                                       aaaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCtt              <  3:1199666/149‑1 (MQ=255)
                                                                                                                                       aaaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTCAGTTGTGGCGCATCGAAAACGCCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCtt              <  4:910207/149‑1 (MQ=255)
                                                                                                                                        aaaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGTGACCACCGTTTCCTCAAGc                         >  2:80265/1‑137 (MQ=255)
                                                                                                                                        aaaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc                         <  1:80265/137‑1 (MQ=255)
                                                                                                                                         aaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGc            >  4:439280/1‑149 (MQ=255)
                                                                                                                                          aaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGcc           >  1:907865/1‑149 (MQ=255)
                                                                                                                                          aaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCgag                                           <  4:1037691/117‑1 (MQ=255)
                                                                                                                                           aaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCg          >  3:705753/1‑149 (MQ=255)
                                                                                                                                            aGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGc                                                     >  1:1287164/1‑105 (MQ=255)
                                                                                                                                               gccgcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc      >  4:87525/1‑149 (MQ=255)
                                                                                                                                               gccgcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGGGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc      >  4:322286/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTC  >  NC_000913/4127104‑4127399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: