Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,822 A→T 5.1% intergenic (+123/‑53) ecnB → / → sugE entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,8220AT 5.1% 621.6 / 17.5 178intergenic (+123/‑53)ecnB/sugEentericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (85/83);  new base T (5/4);  total (90/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 188 total aligned reads displayed.

GCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGT  >  NC_000913/4376674‑4376970
                                                                                                                                                    |                                                                                                                                                    
gcgcAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacga                                                                                                                                                      >  1:1046295/1‑149 (MQ=255)
gcgcAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacga                                                                                                                                                      <  3:792394/149‑1 (MQ=255)
 cgcAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac                                                                                                                                                     <  2:8552/149‑1 (MQ=255)
   cagcaACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCt                                                                                                                                                   <  2:622290/149‑1 (MQ=255)
       aaCGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCaa                                                                                                                                               >  1:575204/1‑149 (MQ=255)
        aCGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCaaa                                                                                                                                              >  1:510101/1‑149 (MQ=255)
          gAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGTCGTCCTGCaaagc                                                                                                                                            <  4:607844/149‑3 (MQ=255)
          gAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACg                                                                                                                                            >  4:1329719/1‑149 (MQ=255)
           aaaGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGc                                                                                                                                           <  2:1292608/149‑1 (MQ=255)
              gcgcAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCctc                                                                                                                                        <  2:822066/149‑1 (MQ=255)
                gcggcaataagcaataaCGGTACGACAGCTGTGTCGTGCCGGTTGTTTTTTCGGCGATAGTCATAAAGGTAATAGTTGAAATTCCCCTTCCACCTGGCAAAATATCCGTTCAACCATCAGCTTGGCAGGACGACCTGCAAACGCCtttt                                                                                                                                      <  3:1043894/146‑1 (MQ=255)
                gcagcaATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCtt                                                                                                                                      <  2:799895/149‑1 (MQ=255)
                     aataagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCAc                                                                                                                                 <  1:1354793/149‑1 (MQ=255)
                       taagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCg                                                                                                                               >  3:826559/1‑149 (MQ=255)
                        aagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCgg                                                                                                                              >  4:911132/1‑149 (MQ=255)
                         agcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCggg                                                                                                                             <  3:375723/149‑1 (MQ=255)
                         agcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCggg                                                                                                                             <  2:1390297/149‑1 (MQ=255)
                            aataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGAc                                                                                                                          <  1:151127/149‑1 (MQ=255)
                            aataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGAc                                                                                                                          >  1:243526/1‑149 (MQ=255)
                             ataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACg                                                                                                                         >  4:118238/1‑149 (MQ=255)
                             ataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACg                                                                                                                         >  3:1104199/1‑149 (MQ=255)
                                aCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGcc                                                                                                                      <  4:598906/149‑1 (MQ=255)
                                   gTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCa                                                                                                                   >  1:492149/1‑149 (MQ=255)
                                    tACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCaa                                                                                                                  >  2:1070784/1‑149 (MQ=255)
                                       gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATtc                                                                                                               >  1:460468/1‑149 (MQ=255)
                                       gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTACACCGGGGACGGCCCCAATtc                                                                                                               >  4:1479655/1‑149 (MQ=255)
                                        aCAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACg                                                                                                                         >  1:33250/1‑138 (MQ=255)
                                            cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCgg                                                                                                          >  2:249175/1‑149 (MQ=255)
                                             tgtgTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGa                                                                                                         >  2:496454/1‑149 (MQ=255)
                                                   gTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGa                                                                                                   >  1:962728/1‑149 (MQ=255)
                                                    tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAATATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGat                                                                                                  >  1:1014630/1‑149 (MQ=255)
                                                    tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGat                                                                                                  >  2:1257517/1‑149 (MQ=255)
                                                         tttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTc                                                                                             >  4:530739/1‑149 (MQ=255)
                                                         tttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATACCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTc                                                                                             >  1:1264032/1‑149 (MQ=255)
                                                           tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                           <  4:1149896/149‑1 (MQ=255)
                                                           tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                           <  1:880878/149‑1 (MQ=255)
                                                           tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                           <  1:237565/149‑1 (MQ=255)
                                                            gtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                          <  1:825575/149‑1 (MQ=255)
                                                            gtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                          <  3:147090/149‑1 (MQ=255)
                                                             ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                           >  1:1307276/1‑147 (MQ=255)
                                                             ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTgg                                                                                         <  2:1046295/149‑1 (MQ=255)
                                                             ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTgg                                                                                         >  3:230352/1‑149 (MQ=255)
                                                              tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                        <  2:163945/149‑1 (MQ=255)
                                                                tttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGAtt                                                                                      <  4:747594/149‑1 (MQ=255)
                                                                   cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATc                                                                                   <  1:687071/149‑1 (MQ=255)
                                                                   cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATc                                                                                   <  4:1207301/149‑1 (MQ=255)
                                                                       gATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAg                                                                               >  2:432984/1‑149 (MQ=255)
                                                                         tAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCGAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGtt                                                                             >  1:147658/1‑149 (MQ=255)
                                                                           gTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTAt                                                                           >  4:790851/1‑149 (MQ=255)
                                                                             cacaAAGGTAATAGTTGAAATTCCCCTGCCACCCGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac                                                                                                                                                     <  1:1194820/73‑1 (MQ=255)
                                                                              acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGc                                                                        >  3:659257/1‑149 (MQ=255)
                                                                                aaaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTg                                                                      >  3:433005/1‑149 (MQ=255)
                                                                                aaaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTg                                                                      >  3:568756/1‑149 (MQ=255)
                                                                                aaaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTg                                                                      >  3:151766/1‑149 (MQ=255)
                                                                                    gTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTct                                                                  >  4:750450/1‑149 (MQ=255)
                                                                                    gTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTct                                                                  <  2:1179988/149‑1 (MQ=255)
                                                                                      aaTAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgc                                                                >  1:1022178/1‑149 (MQ=255)
                                                                                           ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGCTTATCTTCGTTATTGCTGGTCTGCTGGaa                                                           >  2:214732/1‑149 (MQ=255)
                                                                                           ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGaa                                                           >  1:474849/1‑149 (MQ=255)
                                                                                           ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGaa                                                           <  2:1436778/149‑1 (MQ=255)
                                                                                            tGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAg                                                          <  4:221230/149‑1 (MQ=255)
                                                                                              aaaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                        <  2:480989/149‑1 (MQ=255)
                                                                                               aaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTgg                                                       >  2:390976/1‑149 (MQ=255)
                                                                                               aaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTgg                                                       <  3:530739/149‑1 (MQ=255)
                                                                                                aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGt                                                      <  4:343878/149‑1 (MQ=255)
                                                                                                 ttCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTa                                                     <  1:214732/149‑1 (MQ=255)
                                                                                                  tCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTAt                                                    <  3:632919/149‑1 (MQ=255)
                                                                                                     ccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGa                                                                                                   <  4:283217/99‑1 (MQ=255)
                                                                                                     ccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGa                                                                                                   >  3:283217/1‑99 (MQ=255)
                                                                                                        gCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCg                                              >  2:715126/1‑149 (MQ=255)
                                                                                                          cACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGtt                                            <  2:1190316/149‑1 (MQ=255)
                                                                                                           aCCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTg                                           >  1:1556988/1‑149 (MQ=255)
                                                                                                             cTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                         >  1:658202/1‑149 (MQ=255)
                                                                                                             cTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                         <  3:917507/149‑1 (MQ=255)
                                                                                                              tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGTACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGcc                                        >  1:1455556/1‑149 (MQ=255)
                                                                                                              tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGcc                                        <  2:678860/149‑1 (MQ=255)
                                                                                                                   aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaaa                                   >  3:564028/1‑149 (MQ=255)
                                                                                                                   aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaaa                                   <  1:1150401/149‑1 (MQ=255)
                                                                                                                    aaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                  <  2:1007272/149‑1 (MQ=255)
                                                                                                                        tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAc                              <  3:1329719/149‑1 (MQ=255)
                                                                                                                           gTTCAACCATCAGCTTTGCAGGACGACCGGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCa                           >  4:402554/1‑149 (MQ=255)
                                                                                                                            ttCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCAc                          >  4:1465992/1‑149 (MQ=255)
                                                                                                                             tCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAAtata                               <  2:968616/143‑1 (MQ=255)
                                                                                                                              cAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACgg                        >  3:1242618/1‑149 (MQ=255)
                                                                                                                                  cATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCttt                    >  2:521895/1‑149 (MQ=255)
                                                                                                                                   aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa                                                                                <  2:1382944/88‑1 (MQ=255)
                                                                                                                                   aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa                                                                                >  1:1382944/1‑88 (MQ=255)
                                                                                                                                    tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAg                  >  4:525258/1‑149 (MQ=255)
                                                                                                                                      aGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTc                >  4:1029651/1‑149 (MQ=255)
                                                                                                                                        gtttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt              >  1:313208/2‑149 (MQ=255)
                                                                                                                                        gtttGCAGGTCGTCCTGCAAACGCCTCGTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGAGTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt              >  2:553945/2‑149 (MQ=255)
                                                                                                                                         tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt             >  2:1441894/1‑149 (MQ=255)
                                                                                                                                         tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt             >  4:82749/1‑149 (MQ=255)
                                                                                                                                          ttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt            <  2:1538269/149‑1 (MQ=255)
                                                                                                                                          ttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt            <  2:1022178/149‑1 (MQ=255)
                                                                                                                                          ttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt            <  4:1083972/149‑1 (MQ=255)
                                                                                                                                              aGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa                                                                                >  3:1168621/1‑77 (MQ=255)
                                                                                                                                                  cgacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGa    <  4:1337646/149‑1 (MQ=255)
                                                                                                                                                   gacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGTCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATCTACCCACGGCTTTAGTCGTTTGACGCCGAg   >  1:1032029/1‑149 (MQ=255)
                                                                                                                                                    acCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAgt  >  3:782925/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGT  >  NC_000913/4376674‑4376970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: