Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,496,512 C→T 5.8% intergenic (+23/‑163) leuX → / → intB tRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,496,5120CT 5.8% 686.4 / 20.8 208intergenic (+23/‑163)leuX/intBtRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
Reads supporting (aligned to +/- strand):  ref base C (96/100);  new base T (5/7);  total (101/107)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

Only 100 of 209 total aligned reads displayed.

GACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAA  >  NC_000913/4496364‑4496659
                                                                                                                                                    |                                                                                                                                                   
 aCAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt                                                                                                                                                    <  3:18250/149‑1 (MQ=255)
   aagTATGATCTACAACATTTTCCGCATACCGCTTCATTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACTTGCCATTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGAACTCAACTGa                                                                                                                                                  >  2:1153793/3‑149 (MQ=255)
   aGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGa                                                                                                                                                  <  4:552899/149‑1 (MQ=255)
    ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 <  3:243066/149‑1 (MQ=255)
    ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 <  3:576045/149‑1 (MQ=255)
    ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGCGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 <  2:1191805/149‑1 (MQ=255)
       ataATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTc                                                                                                                                              >  2:638177/1‑149 (MQ=255)
        taATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCt                                                                                                                                             >  3:370567/1‑149 (MQ=255)
        taATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCt                                                                                                                                             >  3:959360/1‑149 (MQ=255)
        gaaTCCACACCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAATTAGGTAGACGCATTTCGTTCAAAATCAAGCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGCCAACAAATGTATGTAAATAGACCTCAACTGAGGTCt                                                                                                                                             <  2:1138972/148‑1 (MQ=255)
          aTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttt                                                                                                                                           >  2:1434767/1‑149 (MQ=255)
          aTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttt                                                                                                                                           <  4:1460831/149‑1 (MQ=255)
           tCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtatt                                                                                                                                          <  2:470606/149‑1 (MQ=255)
             cacaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt                                                                                                                                        >  4:1197835/1‑149 (MQ=255)
             cacaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt                                                                                                                                        >  4:298915/1‑149 (MQ=255)
              acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  3:861934/1‑149 (MQ=255)
              acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  1:405694/1‑149 (MQ=255)
              acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  4:477328/1‑149 (MQ=255)
                aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTAt                                                                                                                                     >  3:479105/1‑149 (MQ=255)
                aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTAt                                                                                                                                     >  2:691245/1‑149 (MQ=255)
                aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTAt                                                                                                                                     <  3:1078815/149‑1 (MQ=255)
                  cGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGc                                                                                                                                   >  3:406302/1‑149 (MQ=255)
                  cGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGc                                                                                                                                   <  2:778197/149‑1 (MQ=255)
                   gTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtatttacatact                                                                                                                                  <  1:782882/149‑8 (MQ=255)
                        ccGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCTTGaaa                                                                                                                             <  4:52480/149‑1 (MQ=255)
                        ccGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGaaa                                                                                                                             <  1:1046694/149‑1 (MQ=255)
                            aTACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCa                                                                                                                         >  1:435123/1‑149 (MQ=255)
                               ccTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCa                                                                                                                         <  1:521912/146‑1 (MQ=255)
                               ccTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCa                                                                                                                         >  2:521912/1‑146 (MQ=255)
                                ctctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     >  4:451045/1‑149 (MQ=255)
                                ctctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     >  4:628034/1‑149 (MQ=255)
                                 tctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                    >  3:110295/1‑149 (MQ=255)
                                 tctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                    >  3:1218145/1‑149 (MQ=255)
                                 tctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                    >  2:1385192/1‑149 (MQ=255)
                                  ctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt                                                                                                                   <  4:1077154/149‑1 (MQ=255)
                                   ttCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTta                                                                                                                  >  3:814217/1‑149 (MQ=255)
                                       gTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatcta                                                                                                              <  2:831216/149‑1 (MQ=255)
                                           cGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCtt                                                                                                          >  4:719987/1‑149 (MQ=255)
                                              aGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc                                                                                                       <  1:1385192/149‑1 (MQ=255)
                                                    gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  3:467789/1‑149 (MQ=255)
                                                    gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  1:1591727/1‑149 (MQ=255)
                                                    gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  1:485176/1‑149 (MQ=255)
                                                    gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  3:1248730/1‑149 (MQ=255)
                                                    gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  1:68457/1‑149 (MQ=255)
                                                      aaTCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTatat                                                                                               >  4:1490820/1‑149 (MQ=255)
                                                      aaTCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTatat                                                                                               >  4:1343602/1‑149 (MQ=255)
                                                          ggTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                           <  2:735796/149‑1 (MQ=255)
                                                                    gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAgg                                                                                 >  1:200490/1‑149 (MQ=255)
                                                                    gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAgg                                                                                 <  3:1416747/149‑1 (MQ=255)
                                                                    gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAgg                                                                                 >  3:1058488/1‑149 (MQ=255)
                                                                     ttGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGt                                                                                >  2:329633/1‑149 (MQ=255)
                                                                     ttGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGt                                                                                >  1:1440604/1‑149 (MQ=255)
                                                                       gATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt                                                                                                                   >  3:423625/1‑112 (MQ=255)
                                                                        aTTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat                                                                                                                                           <  1:123397/87‑3 (MQ=255)
                                                                        aTTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat                                                                                                                                           >  2:123397/1‑85 (MQ=255)
                                                                           cAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCg                                                                          >  4:1569480/1‑149 (MQ=255)
                                                                           cAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCg                                                                          <  1:126761/149‑1 (MQ=255)
                                                                             aaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGat                                                                        <  2:1092277/149‑1 (MQ=255)
                                                                              aaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGata                                                                       >  4:1376328/1‑149 (MQ=255)
                                                                               aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                           <  1:1562984/128‑1 (MQ=255)
                                                                               aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGatat                                                                      >  2:745321/1‑149 (MQ=255)
                                                                                   aCCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtat                                                                                                                 <  1:735964/102‑1 (MQ=255)
                                                                                   aCCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAAc                                                                  <  1:1434767/149‑1 (MQ=255)
                                                                                      gTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTa                                                               >  1:1363598/1‑149 (MQ=255)
                                                                                            aTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatct                                                                                                               <  1:240612/95‑1 (MQ=255)
                                                                                             tACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTAc                                                        <  3:628034/149‑1 (MQ=255)
                                                                                                   ccGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACat                                                  <  2:418812/149‑1 (MQ=255)
                                                                                                       ttCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATAAACGTACATCTACCAACATATGt                                              <  2:1591727/149‑1 (MQ=255)
                                                                                                            gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTa                                         <  3:1490820/149‑1 (MQ=255)
                                                                                                             tCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTag                                        <  3:1476882/149‑2 (MQ=255)
                                                                                                             tCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTAc                                        >  1:2649/1‑149 (MQ=255)
                                                                                                               cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAg                                      >  4:364043/1‑149 (MQ=255)
                                                                                                                   cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  1:234753/149‑1 (MQ=255)
                                                                                                                   cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  4:659856/149‑1 (MQ=255)
                                                                                                                    ttCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatgat                                 <  1:138402/149‑1 (MQ=255)
                                                                                                                     tCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAGGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGatgatg                                <  2:1363598/149‑1 (MQ=255)
                                                                                                                     tCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGatgatg                                >  4:827548/1‑149 (MQ=255)
                                                                                                                        gCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGCTACAGATGATGGTa                             <  1:1194339/149‑1 (MQ=255)
                                                                                                                         cACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTTGTACAgatgat                                 <  3:894006/144‑1 (MQ=255)
                                                                                                                         cACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatgat                                 >  4:894006/1‑144 (MQ=255)
                                                                                                                          aCCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     <  2:1449255/59‑1 (MQ=255)
                                                                                                                          aCCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     >  1:1449255/1‑59 (MQ=255)
                                                                                                                          aCCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTAtt                           >  1:101467/1‑149 (MQ=255)
                                                                                                                            cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTc                         >  2:954944/1‑149 (MQ=255)
                                                                                                                               aaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTCTCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCgg                      <  4:869322/149‑1 (MQ=255)
                                                                                                                               aaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCgg                      <  2:101467/149‑1 (MQ=255)
                                                                                                                                     gTAAATAGCCCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGAt                >  1:1532740/1‑149 (MQ=255)
                                                                                                                                     gTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTAc                                                        <  1:1094972/109‑1 (MQ=255)
                                                                                                                                       aaaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATaa              <  3:537630/149‑1 (MQ=255)
                                                                                                                                         aaaGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATg            >  4:212684/3‑149 (MQ=255)
                                                                                                                                          aaGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCgg                      >  4:338943/2‑138 (MQ=255)
                                                                                                                                          aaGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCgg                      <  3:338943/137‑1 (MQ=255)
                                                                                                                                            gACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCtt         <  2:1440604/149‑1 (MQ=255)
                                                                                                                                            gACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCtt         <  3:1343602/149‑1 (MQ=255)
                                                                                                                                             aCCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCaa                                                                   <  2:1522018/90‑1 (MQ=255)
                                                                                                                                              ccTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGt       <  3:719987/149‑1 (MQ=255)
                                                                                                                                               cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTa      >  2:251005/1‑149 (MQ=255)
                                                                                                                                                tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACatat                                                <  2:234979/106‑1 (MQ=255)
                                                                                                                                                tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGt                                          >  4:587962/1‑112 (MQ=255)
                                                                                                                                                tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGt                                          <  3:587962/112‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAA  >  NC_000913/4496364‑4496659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: