Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 847,214 T→A 7.1% intergenic (‑95/+44) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,2140TA 7.1% 596.6 / 26.9 179intergenic (‑95/+44)glnP/glnHglutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (83/82);  new base A (7/6);  total (91/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 181 total aligned reads displayed.

ACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCT  >  NC_000913/847066‑847362
                                                                                                                                                    |                                                                                                                                                    
  cTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAg                                                                                                                                                    <  3:44304/149‑1 (MQ=255)
   ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCTTAATCCTCTCCCCATAgg                                                                                                                                                   <  2:1390146/149‑1 (MQ=255)
   ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAgg                                                                                                                                                   >  4:450917/1‑149 (MQ=255)
      aaTCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGga                                                                                                                                                <  1:1193142/149‑1 (MQ=255)
       aTCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgag                                                                                                                                               >  3:547488/1‑149 (MQ=255)
         cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  3:3582/149‑1 (MQ=255)
         cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  3:329523/149‑1 (MQ=255)
         cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  3:1248921/149‑1 (MQ=255)
           gAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                           <  4:525453/149‑2 (MQ=255)
             aGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGaa                                                                                                                                         <  4:547488/149‑1 (MQ=255)
              gCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAggatta                                                                                                                                        <  3:216140/149‑5 (MQ=255)
              gCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                           <  2:494757/146‑2 (MQ=255)
              gCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                           >  1:494757/1‑145 (MQ=255)
               cGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCCTATGGGGAGAggattag                                                                                                                                       <  1:1059586/149‑6 (MQ=255)
                     ggCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGt                                                                                                                                 <  4:282925/149‑1 (MQ=255)
                           ccAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGttt                                                                                                                           <  2:388390/149‑1 (MQ=255)
                           ccAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGttt                                                                                                                           <  4:1311103/149‑1 (MQ=255)
                           ccAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGttt                                                                                                                           <  1:282253/149‑1 (MQ=255)
                             aGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTa                                                                                                                         <  3:913319/149‑1 (MQ=255)
                               aTGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       <  2:646130/149‑1 (MQ=255)
                               aTGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       <  2:1177172/149‑1 (MQ=255)
                                tGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAg                                                                                                                      >  4:1538860/1‑149 (MQ=255)
                                      tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGc                                                                                                                                     <  1:661007/128‑1 (MQ=255)
                                      tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       <  2:824846/142‑1 (MQ=255)
                                           tCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGtta                                                                                                           >  2:213365/1‑149 (MQ=255)
                                           tCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGtta                                                                                                           >  3:740434/1‑149 (MQ=255)
                                              aaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAggg                                                                                                                                                  <  1:1181333/107‑1 (MQ=255)
                                              aaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAggg                                                                                                                                                  >  2:1181333/1‑107 (MQ=255)
                                              aaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTATCGttatta                                                                                                        >  2:1372474/1‑149 (MQ=255)
                                               aCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTtattat                                                                                                       >  2:275908/1‑149 (MQ=255)
                                                cTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGATTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAggg                                                                                                                                           <  3:1366770/112‑1 (MQ=255)
                                                   cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCg                                                                                                   <  3:825008/149‑1 (MQ=255)
                                                   cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCg                                                                                                   >  2:1009137/1‑149 (MQ=255)
                                                     tatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTc                                                                                                    >  1:1515667/1‑146 (MQ=255)
                                                     tatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTc                                                                                                    <  2:1515667/146‑1 (MQ=255)
                                                     tatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGt                                                                                                 <  1:911747/149‑1 (MQ=255)
                                                        gttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGgaga                                                                                                                                              >  4:1032389/1‑101 (MQ=255)
                                                        gttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGgaga                                                                                                                                              <  3:1032389/101‑1 (MQ=255)
                                                           gttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgaga                                                                                                                                              <  2:359586/98‑1 (MQ=255)
                                                           gttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTa                                                                                           >  2:1244619/1‑149 (MQ=255)
                                                            ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                          >  2:675814/1‑149 (MQ=255)
                                                                    aCCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAt                                                                                  <  3:437573/149‑1 (MQ=255)
                                                                     ccGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtt                                                                                 >  3:1517598/1‑149 (MQ=255)
                                                                       gtggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtttt                                                                               <  4:552259/149‑1 (MQ=255)
                                                                         ggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtttttt                                                                             <  1:516001/149‑1 (MQ=255)
                                                                         ggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtttttt                                                                             <  3:661875/149‑1 (MQ=255)
                                                                            gtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTa                                                                          >  2:526966/1‑149 (MQ=255)
                                                                             tAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAg                                                                         <  2:1466036/149‑1 (MQ=255)
                                                                               aataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatt                                                                                                         <  4:423993/115‑1 (MQ=255)
                                                                               aataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAt                                                                       <  2:983194/149‑1 (MQ=255)
                                                                               aataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAt                                                                       >  4:680220/1‑149 (MQ=255)
                                                                                 taatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAttt                                                                     >  3:221470/1‑149 (MQ=255)
                                                                                  aatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAg                                                                                                                      <  4:1402709/99‑1 (MQ=255)
                                                                                    tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGaa                                                                                      <  2:244956/129‑1 (MQ=255)
                                                                                    tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGaa                                                                                      >  1:244956/1‑129 (MQ=255)
                                                                                    tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  1:211788/1‑149 (MQ=255)
                                                                                    tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  3:335189/1‑149 (MQ=255)
                                                                                    tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  2:1317180/1‑149 (MQ=255)
                                                                                     cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                                 <  3:94849/149‑1 (MQ=255)
                                                                                     cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                                 <  1:1372474/149‑1 (MQ=255)
                                                                                     cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                                 <  2:52145/149‑1 (MQ=255)
                                                                                        aTTCAGGATAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTACATTTCGTTGTa                                                              <  3:1356720/149‑1 (MQ=255)
                                                                                         ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTaa                                                             >  4:928505/1‑149 (MQ=255)
                                                                                         ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTaa                                                             <  1:719142/149‑1 (MQ=255)
                                                                                         ttCAGGGGAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTaa                                                             >  4:1424316/1‑149 (MQ=255)
                                                                                              ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                                        <  4:293818/149‑1 (MQ=255)
                                                                                                taattaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGt                                                      >  1:688019/1‑149 (MQ=255)
                                                                                                   ttaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtctc                                                  >  4:749909/1‑149 (MQ=255)
                                                                                                    taatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGCGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtctc                                                  <  1:275908/149‑1 (MQ=255)
                                                                                                         gCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCa                                             <  3:399063/149‑1 (MQ=255)
                                                                                                              ggAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       <  4:9573/70‑1 (MQ=255)
                                                                                                                aGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGtttt                                      <  2:248762/149‑1 (MQ=255)
                                                                                                                    ggTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAc                                  <  2:211788/149‑1 (MQ=255)
                                                                                                                    ggTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAc                                  <  1:1009137/149‑1 (MQ=255)
                                                                                                                     gTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAcg                                 >  1:596810/1‑149 (MQ=255)
                                                                                                                     gTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAcg                                 >  3:168630/1‑149 (MQ=255)
                                                                                                                      tttGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAcgc                                <  3:857342/149‑1 (MQ=255)
                                                                                                                       ttGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACgcgc                              <  3:749909/149‑1 (MQ=255)
                                                                                                                            ccccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGtt                          >  1:122201/1‑149 (MQ=255)
                                                                                                                            ccccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGtt                          >  3:373018/1‑149 (MQ=255)
                                                                                                                             cccTCACCCTACTCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTg                         >  3:889274/1‑149 (MQ=255)
                                                                                                                              ccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGa                        <  3:280365/149‑1 (MQ=255)
                                                                                                                              ccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGa                        <  1:1317180/149‑1 (MQ=255)
                                                                                                                               cTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAc                                  >  4:210155/1‑138 (MQ=255)
                                                                                                                               cTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGAc                       >  2:1592709/1‑149 (MQ=255)
                                                                                                                                tCACCCTAATCCTCTCCCCATAGGGGAGAGGGCACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGAGCCGTTGACt                      >  4:1001797/1‑149 (MQ=255)
                                                                                                                                    ccTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACttt                    >  3:1422554/1‑147 (MQ=255)
                                                                                                                                      tAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCa                >  4:1271522/1‑149 (MQ=255)
                                                                                                                                         tCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatta                                                                                                        <  3:1137477/58‑1 (MQ=255)
                                                                                                                                           ctctCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGaa                                                                                      >  3:1182916/1‑74 (MQ=39)
                                                                                                                                           ctctCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg           >  4:306932/1‑149 (MQ=255)
                                                                                                                                           ctctCCCCGATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGCGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg           >  4:487687/1‑149 (MQ=255)
                                                                                                                                           ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg           >  4:414545/1‑149 (MQ=255)
                                                                                                                                            tctcCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTAc                                                                                                                        >  2:1389864/1‑39 (MQ=21)
                                                                                                                                             ctcCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCt         >  3:1099441/1‑149 (MQ=255)
                                                                                                                                                cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      <  4:197537/149‑1 (MQ=255)
                                                                                                                                                cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      >  1:363568/1‑149 (MQ=255)
                                                                                                                                                cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATCTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      <  4:1143783/149‑1 (MQ=255)
                                                                                                                                                  cATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgtcg    >  4:1282489/1‑149 (MQ=255)
                                                                                                                                                   aTAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                                <  3:459415/56‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCT  >  NC_000913/847066‑847362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: