Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C96.7% 288.4 / 2.2 93intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (37/52);  total (39/54)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
*NC_0009134,296,3802.G96.7% 283.5 / 2.0 93intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (37/52);  total (39/54)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

ACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGT  >  NC_000913/4296166‑4296644
                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                            
aCATCAACGCCTGATGCGACGCTTAACG‑‑‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACG‑‑‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGTTTTTGTAGGTCGGATAAGGCGTCT‑‑ACGC‑‑CG‑CATCCGACATCCATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTCTCTTAACCGtt                                                                                                                                                                                                           >  3:173348/1‑275 (MQ=11)
           tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                                                                                                                                                                     >  2:464148/1‑276 (MQ=9)
                  aCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CGACG‑CATCCGACATAAATTCCTTATGCGACGCTTGCCGTGTCTTATTAGGCCTATTTTAACCGCTGGTTAATTTTTc                                                                                                                                                                                               >  2:630518/1‑275 (MQ=11)
                    gCTTAACGCGTCTTATCCGGCCGACGCCAGACAGTGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  2:60826/276‑1 (MQ=11)
                    cctggaCGCGTGTGAGCAGGCATACGACAGACAGCGCAAAAGCCTGATTGAGGGGGAGTTTGTAGGTCGGGTAAGGCGTTTATGCCGCATCCGAGATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  2:351114/271‑1 (MQ=11)
                       tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  3:471386/270‑1 (MQ=255)
                           ggttCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                                                                     <  2:280157/273‑1 (MQ=255)
                                ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCCTGCCCCGTCTTATTAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTcc                                                                                                                                                                                     >  3:88749/1‑270 (MQ=255)
                                ttATCAGGCCGAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  2:48897/275‑1 (MQ=255)
                                 tataAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  1:157337/274‑1 (MQ=255)
                                    gaGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                                                                                                                                                       <  1:88749/264‑1 (MQ=255)
                                        ccTACGCACGACAGCGGCGTAGCATGATTTATCGTGTTTTTGTAGGTCGAATAAGTCGTTTATGCCGCATCCTACATCAACGCCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                                                                                                                                                                         <  1:426771/275‑1 (MQ=14)
                                          ttcGCCAGTCAGAGCAAAAGCCTGATTGAGGGTGATTGTGTAGGTCGGATAAGACGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  2:191509/273‑1 (MQ=14)
                                          taagccgacaaGCGTAATAGCCTGATTTATGGTGATGTTGTAGGTCGGATAAGGCTGTTATGCCGAATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  4:600499/265‑1 (MQ=14)
                                                 gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATTCCATACCAATGCCTGCTGCGTCGCTTGCAGCGTCTTACCAGGCCTATCTCAACCGTTGGTTAGTTTCTTGAACTCTCCGCTCTCGCTATCAACCCCtcca                                                                                                                                                                >  4:614494/1‑272 (MQ=11)
                                                     gcgcAATGTCCTGATTTAGCGTGAGTGTGTAGGTCGGATAAGGCGTTTATGCCGCAACCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATTCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                                                             <  2:535006/274‑1 (MQ=17)
                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTCCG‑‑C‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCTTCTTATGAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTGCACACCCGGTATCAAGCCCGTCCCTACAg                                                                                                                                                          >  4:6972/1‑276 (MQ=14)
                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                                                                                                                           >  1:638314/1‑275 (MQ=255)
                                                        taagaGCCTGATTTAGCGTTATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                        <  2:555992/272‑1 (MQ=255)
                                                        cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                        <  2:619414/276‑1 (MQ=255)
                                                         aatatgcTGATTTGGCGTGATTTTGTAGGTGGGATAAGTCGTTTAGGCCGCAGCCGAAATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                        <  3:276135/269‑1 (MQ=17)
                                                              ccagATTTAGCATGATTTGGGTGGTCGGTAAAGGCATTTATGCCACATCAGCCGTCAACACCTGATGCGACACTTAACGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATACCCTGATTTCGCGTGATTTTGTAGGTCAGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                                                                   <  3:129701/272‑1 (MQ=17)
                                                              ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAAAGCTAAGGt                                                                                                                                                   >  2:298481/1‑275 (MQ=255)
                                                                tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAAACCTTCCTTACACCCTAGGtt                                                                                                                                                  >  4:219354/1‑274 (MQ=17)
                                                                 gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACATCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                                                                   <  1:412220/272‑1 (MQ=255)
                                                                   tttAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCTGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                                                                   <  2:219354/270‑1 (MQ=255)
                                                                        cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGc                                                                                                                                        >  2:72260/1‑276 (MQ=255)
                                                                        cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGATCAACCAGttc                                                                                                                                        >  2:464132/1‑274 (MQ=255)
                                                                           gATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                                                                      <  2:58293/275‑1 (MQ=255)
                                                                            aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACATCTACGGTCAAACCaattcaac                                                                                                                                     >  4:513569/1‑268 (MQ=17)
                                                                            aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATAAAACCCTTCCATACAGCCTAGGTTCAAcactga                                                                                                                                         >  4:58293/1‑266 (MQ=255)
                                                                               ttGTAGGTCGGAT‑AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                                                 <  3:137768/275‑1 (MQ=255)
                                                                                ggTAGGTCTGATAAGGCGGTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGAGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                                                 <  2:5636/274‑1 (MQ=255)
                                                                                  gtgatcgatgaaGGCTGTTATACCGCATCCGACAGCACGGCCTGGTTGGAGGCTGAACGGGTCTTATAAGGCGTAAGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCtttt                                                                                                                              <  4:613977/266‑1 (MQ=18)
                                                                                      tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCAAACTCTTcc                                                                                                                                                                                     >  3:370918/1‑216 (MQ=255)
                                                                                          aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                 <  1:370918/217‑1 (MQ=255)
                                                                                            aaGGCGTTTAAGCCGCGTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAGCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt                                                                                                                    <  2:425629/276‑1 (MQ=255)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGTCATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTAACCGTTGCTTAATTTTTCAACCTCTTCACACCCGGTCTCTAACCCTTCACTCCATCCAAGGTTCACCATGTTCAGCGCCTTTTCCTTCTTCTTCTCgctca                                                                                                            >  4:460384/1‑272 (MQ=17)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTATTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTCCAGCGCCTTTTGCTTGTTCTTCTCGCTa                                                                                                             >  2:257536/1‑275 (MQ=255)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCCGGTTCAACCATTGCAGCGCATTTTGTTTGTTCTTCTCGCTat                                                                                                            >  4:30581/1‑275 (MQ=18)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGCTCAGTTTCAACCATTGCAGCCCCTTTTGCTTGTTCTTCTCGaca                                                                                                             >  4:415531/1‑273 (MQ=255)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAAACCCGGTATCAAACCCTTCCATACAGCCCAGGTTCAACCAGTTCAGCGCCTTTTGCTTTTTCTTCTCGaa                                                                                                              >  3:711082/1‑273 (MQ=255)
                                                                                                    tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTa                                                                                                             >  2:589304/1‑275 (MQ=255)
                                                                                                       gCCGCATCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                                                           <  1:702564/274‑1 (MQ=255)
                                                                                                        cggCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                                                           <  1:178683/271‑1 (MQ=255)
                                                                                                              ccGAGATCAACGCCTGATGCGACGCTTAACGCGTCTAATCATGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTGAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                   <  4:464148/275‑1 (MQ=255)
                                                                                                              ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                   <  2:124904/275‑1 (MQ=255)
                                                                                                               cGAAATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCGTAAGCCAGACAGCGTCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCATCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                   <  1:229608/274‑1 (MQ=21)
                                                                                                                 acctccaCGCCTGATGCGACGCTTAGCGCGTCTTATCAGGCCTATGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGGCTGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAcc                                                                                               <  4:464132/270‑1 (MQ=25)
                                                                                                                   aTCACCGCCTGATGCGACGCTTAACGCGTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGt                                                                                             <  2:432777/276‑1 (MQ=255)
                                                                                                                    taaaCGCAGGATACGACGCTTACCGTGTCGTATAAAGCC‑ACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGGAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGTGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGttt                                                                                           <  2:314085/274‑1 (MQ=25)
                                                                                                                     cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGa                                                                                                               >  3:178683/1‑256 (MQ=255)
                                                                                                                       aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCCGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGtttc                                                                                          >  4:113330/1‑274 (MQ=255)
                                                                                                                        cGCCGGATGAGACTCTTACCGCGTTTTATAAGGGCTACGCCAGACAGCACAATAGCCTGATTTAGCGTGATTTTTGAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                        <  4:274845/276‑1 (MQ=25)
                                                                                                                         gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                        <  2:68202/275‑1 (MQ=255)
                                                                                                                              aggCGACGATTAACGCGTATTATCAGGCCTACGGCAGACAGCGCAATAGCCTGATGTAGCGTGATGTTGTAGGTCGGAGAAGGCGTTT‑‑ACGC‑‑CG‑GATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGCCCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAca‑t                                                                                  <  1:107231/273‑1 (MQ=25)
                                                                                                                                gctaCGCTTAACGCGTCTCATAAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACTGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTGCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAca‑tca                                                                                <  1:488070/272‑1 (MQ=25)
                                                                                                                                  taCGCTTAACGGGGCTTATCAGGCCCACGCAAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTc                                                                             <  4:257536/275‑1 (MQ=25)
                                                                                                                                       ttAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGcc                                                                        <  4:589304/276‑1 (MQ=255)
                                                                                                                                         taCGCTTGTTATCCGGCCTACGCCAGACAGCGCGATGGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGccc                                                                       <  3:504908/274‑1 (MQ=37)
                                                                                                                                         aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTATTCTTCTCGATAAACCCTTCTTCACCGTTTAAGACCA‑TCATCCACGccc                                                                       >  2:414572/1‑275 (MQ=25)
                                                                                                                                          aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCa                                                                      <  1:91019/275‑1 (MQ=255)
                                                                                                                                          aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCa                                                                      <  1:711082/275‑1 (MQ=255)
                                                                                                                                              gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTCCTTGTTCTTCTCGATAACACCTTCTTCACCGTTTACGAACA‑TCATTCCCGCCCACTa                                                                   >  2:26309/1‑274 (MQ=255)
                                                                                                                                               tgttATCACGCCGACGCCAGACAGCGCAATAGCCTTAGTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTAc                                                                  <  3:429334/272‑1 (MQ=37)
                                                                                                                                               tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCTATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTAc                                                                  >  1:682108/1‑274 (MQ=255)
                                                                                                                                               tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACACTTCCCTACAGCTCCGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTCATCGCTAAAACCTTCTTCCCCGATTCA‑AACACTCATTCCACCCCAATaat                                                                 >  2:665451/1‑273 (MQ=21)
                                                                                                                                                   aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGaa                                                            >  2:632139/1‑276 (MQ=255)
                                                                                                                                                       tgccggcagcAGAAAGCGCAAGAGCCTGATTTGGCGTGATTTTGTCGGTCGGATAATGCGTTT‑‑ACGC‑‑CG‑CATCGGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGNTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAAc                                                        <  4:369700/267‑1 (MQ=37)
                                                                                                                                                        gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAAc                                                        >  3:431809/1‑275 (MQ=255)
                                                                                                                                                        gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAAc                                                        <  1:454510/275‑1 (MQ=255)
                                                                                                                                                             cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCTATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCTAACTCTTCCTTATTGTTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCCCT‑AAACCTTCTTCACCGTTTATGAACT‑TCATTCCCGCCCAGTACCCGGAATACCCGGtt                                                   >  3:296176/1‑274 (MQ=25)
                                                                                                                                                              gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGtt                                                   <  3:638314/274‑1 (MQ=255)
                                                                                                                                                               ccAGACAGCGCAATAGCCTGATTTAGGATGATGTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCg                                                 <  1:671375/275‑1 (MQ=255)
                                                                                                                                                                  gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCCTTCCCGCCAAGTACTCGGAATAACCGTTTCgggc                                              >  4:655112/1‑274 (MQ=255)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                         >  1:693564/1‑275 (MQ=255)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTTTCGATAAAACCTTCTTCCCCGTTTAAGAACA‑TCATTCCCGCCCAGTCCTCGGAATAACCGGTTCGGTCAATTg                                         >  3:550384/1‑275 (MQ=37)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                         >  1:215500/1‑275 (MQ=255)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTTCCCCCCACTACTCAGAATAACCGCTTCGGAAAATTGc                                        >  4:207580/1‑276 (MQ=37)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTg                                         >  1:664428/1‑275 (MQ=255)
                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGc                                        >  2:70363/1‑276 (MQ=255)
                                                                                                                                                                            taaccTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag                                    <  1:276121/272‑1 (MQ=255)
                                                                                                                                                                            gaGCCTTATTTAGCTGGATTGTGTGTGTCGTTTAAGGCGTTT‑‑ACGC‑‑CG‑CCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAATAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag                                    <  1:649591/274‑1 (MQ=38)
                                                                                                                                                                                  gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCACCCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTCAGAACA‑TCCTTCCCGCCCAGTACTCGACATCACCGTTTCGGGAAATTCCAGAGCTTCGt                              >  3:391432/1‑275 (MQ=37)
                                                                                                                                                                                    ttttGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CC‑CCACCCACATCAATGCCTGATGCGACGCTTGCCGCCTTTTATCAGGCCTATCCTAACCGTTGGTTAATTTTTCCAACTCTTCCCCCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGttt                            <  1:173348/275‑1 (MQ=38)
                                                                                                                                                                                         cGTGATTTTGTAGGACGGATAAGGCGTTT‑‑ACGC‑‑CT‑GATCCAACAGCAATGCCTGATGCGACGCATGCCGCGTCTTAACAGGCCTATCTTAACCGTGGGTTAATTTTTCAAACTCTACACACCCAGTATCAAACCCATCCATACGGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCAGAATAACCGGTTCGAGAAATTGCAGAGCTGCGTTTGAAATa                      <  2:293745/276‑1 (MQ=255)
                                                                                                                                                                                               tttGTAGGTCGGCTAAGGCGTTT‑‑ACGC‑‑CC‑CATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGt                <  4:632139/276‑1 (MQ=255)
                                                                                                                                                                                                   tAGGTCGGATAAGGCGTTT‑‑ATGC‑‑CG‑CATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGcc            <  2:683625/276‑1 (MQ=255)
                                                                                                                                                                                                   tAGGTCGGATAAGGCGTTT‑‑ACTC‑‑CT‑CATCCGAAATCAATGCTGGATGCGACGCTTGCCGCGTCTTATCAGGCCGATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGcc            <  4:508614/276‑1 (MQ=255)
                                                                                                                                                                                                        cgtgtAAGGCGTTT‑‑A‑‑CT‑CGTCATCCGACACGCATGCGTGATGCGTCGCTTGCCACGTCTTATCAGGCCTATCTTAGCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTCGCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTc       <  4:15740/272‑1 (MQ=255)
                                                                                                                                                                                                        cGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTc       >  2:164986/1‑276 (MQ=255)
                                                                                                                                                                                                          gATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAAACGGTTCGGGAAATTGCAGAGATGCGTTTGAAATACAAGGTTGCCTTGTCAt     >  2:54653/1‑276 (MQ=255)
                                                                                                                                                                                                             aaGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAACAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgt  <  2:448080/276‑1 (MQ=255)
                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                            
ACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACA‑TCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGT  >  NC_000913/4296166‑4296644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑