Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,875,521	0	T	C	27.3%	93.4 / 21.1	67	A28A (GCT→GCC)	yidX	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (28/20);  new base C (18/0);  total (47/20)
Fisher's exact test for biased strand distribution p-value = 6.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACT  >  NC_000913/3875263‑3875795                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   cgtgTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTAGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCGATACCGc                                                                                                                                                                                                                                                                   >  2:9380/1‑276 (MQ=255)       atttagtaCAAACTTATAATTTTGTACTAAAATTTAAATCACAAAAAGAACAAATCAAAAAAAAATGAAAGGCGTCGGGAAAAAAACTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAATAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAggg                                                                                                                                                                                                                                                             <  4:431816/273‑1 (MQ=255)            gtaCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCAGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTCTCCCTTAACGCAAAGTGTatt                                                                                                                                                                                                                                                         >  1:445108/1‑273 (MQ=255)                aaaaTTAAATTTGTGTACTACAATTTAAATCAAAAAAAGAACAATGCATAAAAAAAAGACATGCGTCGGGCAGAAATATGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAgg                                                                                                                                                                                                                                                    <  4:28175/276‑1 (MQ=255)                           gttggAATAAAATTTAGATCACAAAAAGAACAATTCATAAAAAAATTACATGCGTCGGCAATAAATCTGAAAAGGGATATCATGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGACTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAt                                                                                                                                                                                                                                         <  4:297244/276‑1 (MQ=255)                            ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGTTTTCCTATACAGCAAAGGGTATTGCAGGAAAGTATAAATc                                                                                                                                                                                                                                        >  2:605827/1‑276 (MQ=255)                             tgtACTACAAATTAGATAACACAAAGAACAATGCACAAAAAAATGACAGGGGGTGGGTAGACAACTGAAAAGGTATATCAGGAGCTAAGCAGGAGGGAAAGAGGAGTATGAGTTCAACGGGTTAGCTACTAGTTTAATGGATTAATCATGAAGTTGAATTTTAAGGGATTTTATAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGTCTGTATCTCGTATGCTCTGGTTTCCCAGACCGCAAAGGTTAGTTCCGGAAAGTATCAATCg                                                                                                                                                                                                                                       <  4:582382/276‑1 (MQ=255)                              gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATc                                                                                                                                                                                                                                        >  1:591046/1‑274 (MQ=255)                                    cAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTATACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAg                                                                                                                                                                                                                                >  2:236316/1‑276 (MQ=255)                                    cAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTTTCCTTGACAAAAAAGGATAGTACAGGAAAGTAACAATCGCAGTc                                                                                                                                                                                                                                  >  1:726837/1‑274 (MQ=255)                                    cAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCAACTGGCGCTGAGGCTTTCAGTCTGTATCTCGTATGCCCTGTTTTGCTTTCCCGAAAAGGGTAGTACAGTAAAGTATCAAttggagcaa                                                                                                                                                                                                                                 >  1:666558/1‑267 (MQ=255)                                       tttAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTAAGac                                                                                                                                                                                                                              >  1:553981/1‑275 (MQ=255)                                         tAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTTTGCCTTACAGAAAAGGGTAGTTCAGGAAAGTATCTATCGCATTCATACAcc                                                                                                                                                                                                                           >  2:202646/1‑276 (MQ=255)                                            aTCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCGCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCTGAGTCAGACACCATc                                                                                                                                                                                                                        >  4:470271/1‑276 (MQ=255)                                                   aaaGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTggg                                                                                                                                                                                                                  <  1:491789/275‑1 (MQ=255)                                                           aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCCATACCTAAAAGTGAAGTTCAGGAAAGTTTCAATCGCATCCATACACCATCACTGGGcactccga                                                                                                                                                                                                          >  1:498637/1‑268 (MQ=255)                                                              cATTAAAAAATGAAATGCGTAAGGCAGAAATCTGAAAAGGTATATCAGGCACTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCAGGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCa                                                                                                                                                                                                      <  4:162702/276‑1 (MQ=255)                                                                 aaaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATCCCGCAAAGGGTAGTTCCGGAAAGTATCAAGCGCAGTCAGACACCATCCATGGGGTACCGAAGGCaaa                                                                                                                                                                                                    >  3:472046/1‑275 (MQ=255)                                                                                 tCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGCTCGTAATGGAACaa                                                                                                                                                                                   >  2:90642/1‑276 (MQ=255)                                                                                 tCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCACAACTGGGCTATCGCAGGCCAAAGATAGTAATGGAACaa                                                                                                                                                                                   >  2:416592/1‑276 (MQ=255)                                                                                 tCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCTTACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATATTAATGGAACa                                                                                                                                                                                    >  1:631207/1‑275 (MQ=255)                                                                                   gggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTCTACCACAAAGGGTAGTTCAGTAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATAGCAGGCAAAAGATAGTAATGGAACaaa                                                                                                                                                                                  >  1:478646/1‑275 (MQ=255)                                                                                    ggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATAGCAGTCAGACACCAGCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaaaa                                                                                                                                                                                 >  1:89997/1‑275 (MQ=255)                                                                                    ggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCTTCTCCCTTACAGCAAAGAGTATTTCAGGAAAGTATCAATCGCAGTCAGACACCATCCCTGGGCTATCGCCGGCAACAGTTAGTACTGGAACAAAAg                                                                                                                                                                                >  2:600904/1‑276 (MQ=255)                                                                                         aaaTCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCTGGAAAGTATCAATCGCAGTCATACCCCATCACTGGTCTTTCGCAGGCACAAGATAGTAATGGAACAAAAGGCTAt                                                                                                                                                                           >  4:64099/1‑276 (MQ=255)                                                                                                               cgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCTATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGATATTGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCgt                                                                                                                                                        >  1:160984/1‑272 (MQ=255)                                                                                                                 cTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAGGATAGTAATGGAACAAACGGCTATGTTTTTGTTGGGGAATCGCTGGAt                                                                                                                                                   >  4:152827/1‑276 (MQ=255)                                                                                                                    aagaggaggGAAAGGAGTGTATGCTTTACACGGCTTAAGTACTGGTTATAAGGATTAATCATGGAGTTGAATTTTAAGGGATTTTATAGGGCTGCCGTTTTATTCCGACTGGCGCTGATGCTTTCAGGCTGTATCGCGTATGCTCTGGGGTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                   <  3:45800/270‑1 (MQ=255)                                                                                                                      cAGGAGGGAAAGAAGGTGATTCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATTAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTAATGCTTTCAGACTGTATCTCGAATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAc                                                                                                                                                <  3:89997/274‑1 (MQ=255)                                                                                                                              aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTACGTTTTTGTATGGGAATCGCTGGATTACCTTAtgcatg                                                                                                                                      >  4:66951/1‑271 (MQ=255)                                                                                                                                       tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAcc                                                                                                                                               >  1:421290/1‑258 (MQ=255)                                                                                                                                       tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCg                                                                                                                             >  2:126712/1‑276 (MQ=255)                                                                                                                                           cTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTAt                                                                                                                                           <  3:421290/258‑1 (MQ=255)                                                                                                                                            tttCAAAGGCTTAGATACTAGTTTAAAAGATTAATAATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTTCCGGTTTATTCCCACTGTCGCTGATGCGTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                         <  3:397291/275‑1 (MQ=255)                                                                                                                                            tgtaaaCGTCTTATCTACTTGTTTAAAGGATGAAGCATGAAGTTTAATGTTAAGGGATTTTTTAAGGCTGCCGGTGTATTCCCACTGTCGCTGATGCTTTCAGGCTGTATCTCGGATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                         <  4:90642/271‑1 (MQ=255)                                                                                                                                             ttCAACTGCTTAGCTACTTGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTTCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                         <  3:134357/274‑1 (MQ=255)                                                                                                                                             ttCAACGGGTTAGATTATCGTTTAAAGAATTAATCATGAAGTTGAATTTTAAGGGATTGTTTAAGGCTGCCGATTTATTGCCCCATGGGCTGAGGCTTTCAGGAGATAGCTCGGATGCTCTGGATACCCATAATGTAAAGGGTAGTTCAGGAAAATATCAAACGCAGTCAGACACCAGCACTGGGCTATAGCAGGCAAAAGATAGTAATGGTACAAAAGGCTATGTTTTTGTATGTGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                         <  3:554340/274‑1 (MQ=255)                                                                                                                                               cAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACAcc                                                                                                                                                                                                                           >  1:407082/1‑174 (MQ=255)                                                                                                                                                   ggCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCa                                                                                                                                                                                                                       <  3:407082/174‑1 (MQ=255)                                                                                                                                                     cTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACAGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTTGATTACCTTATCACCGATGGTGCCGATGACATCGTTaa                                                                                                                >  2:432156/1‑275 (MQ=255)                                                                                                                                                     cTTAGCTACACGTATAAAGGATTAATCAGGAAGTTGAAGTTTAAGGGATTTTTTATGGCTGCCGGTTTATTACGACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                                                 <  2:64099/274‑1 (MQ=255)                                                                                                                                                      ctAGCTAATCGTTTAAAGGATTAATCATGAAGTTGAATGTTAAGGGATTTGTTAAGGATTCTGTTTTATTCCCACTGGCGATGATGCTTTCAGGCTGTATCTCGTATGATCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAg                                                                                                               <  3:591046/274‑1 (MQ=255)                                                                                                                                                                   tAAAGGATTAAGCATGAAGTTGTATTTGAAGGGATTGTTTAAGGCTGCGGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACAAGCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATc                                                                                                  <  2:470271/275‑1 (MQ=255)                                                                                                                                                                          ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCCGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTACCGATGACATCGTTAAGATGCTAAATTATCCAGAACtt                                                                                          >  4:564500/1‑276 (MQ=255)                                                                                                                                                                                  gAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATAGCTGGCTTACCTTAACACTGATGGTTCCGCAGCCAACGTTAAGATGCTCAATGCTCCCATACCTAACCGGcc                                                                                  >  4:110206/1‑275 (MQ=255)                                                                                                                                                                                  gAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGca                                                                                  >  2:391491/1‑276 (MQ=255)                                                                                                                                                                                   aaGTTGCAATGTAAGGGATTTTTTCAGGCTCACGGTTTATTACCACTGGGGATGATGCTTTCAGGCTGGATCACGTATGCTCTGGTTTCCAAGACCACAAAGGGTAGGTCAGGAAAGTATCAATCGCAGTCAGACGCCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGcac                                                                                 <  4:416592/276‑1 (MQ=255)                                                                                                                                                                                        gAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAGAAAAGATTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAACGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTACGGAGGTCAATGATCCATCACTTACCCGGCACAata                                                                             >  1:272731/1‑275 (MQ=255)                                                                                                                                                                                        gAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCCCAatat                                                                            >  2:525763/1‑276 (MQ=255)                                                                                                                                                                                         aaTTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTCTCCCATACAGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGTCGATGACATCGTTAAGATGCTCCATGATCCAGCACTTAACCGGCACAATAtt                                                                           >  2:561898/1‑276 (MQ=255)                                                                                                                                                                                              tAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGCCATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAAAATTCAgg                                                                       >  1:27695/1‑275 (MQ=255)                                                                                                                                                                                              tAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGTGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGt                                                                      >  2:254796/1‑276 (MQ=255)                                                                                                                                                                                                             ggCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTCTGCCATACCGCAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCCCTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTCTGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGTCGATGACATCCTTAAGATGCTCAATGATCCAGCCCTTAACCGGCACAATATTCAGTTTGCCGCTGACGCAAg                                                       >  2:661478/1‑276 (MQ=255)                                                                                                                                                                                                                 gCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACACTGGTAAGATGCTCAATGATCCAGCACTTAACAGACACAATATTCAGGTTGCCGATGACGCAAGAttg                                                   >  4:56911/1‑275 (MQ=255)                                                                                                                                                                                                                  ccGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTTGATTACCTTATCACTTATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGtttt                                                   >  1:430855/1‑275 (MQ=255)                                                                                                                                                                                                                  ccGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCCTTAAAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTTCCGATGACGCATGACTTg                                                  >  2:573834/1‑276 (MQ=255)                                                                                                                                                                                                                               aCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGCTAAGATGCTAAAGGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTTGTTTAAATgggg                                      >  3:291835/1‑275 (MQ=255)                                                                                                                                                                                                                               aCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTCGGCTATCGCAGGCAAACGATAGTAATGGAACCAAAGGCTATGTTTTTGTAGGGGAATCGATGGATTATCTTATCACTTATGGTGCCGATTACATCGTTAAGCTTCTCAATGCTCCAGCACTTAATCGGCCCAATATTCATGTTGCCTCTTGCCCAAGATTTTTTTTTAATACgtg                                     >  4:298416/1‑274 (MQ=255)                                                                                                                                                                                                                               aCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTCTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATGACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGAACAATATTCAGGTTGCCGATGACTCAAGATTTGTTTTAAATGCgg                                      >  1:78641/1‑275 (MQ=255)                                                                                                                                                                                                                                     gcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGCGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCTAAAGATCGTATTGGAACAAAAGGCTATGTTTTTGTAGGGGAATTGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGAGTCTCAATGAACCAGCACTTAACCGGCACCATATTCAGGATGCCGATGCCGCATGATGTGGTTTACATGCGTGGacaaa                               >  2:154176/1‑276 (MQ=255)                                                                                                                                                                                                                                         aTGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGa                                                                                                                            >  1:441123/1‑179 (MQ=255)                                                                                                                                                                                                                                             tttCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGAc                                                                                                                        <  3:441123/179‑1 (MQ=255)                                                                                                                                                                                                                                                   gCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAAAAATTTACCgg                   >  1:237302/1‑274 (MQ=255)                                                                                                                                                                                                                                                      gTATCTCGTATGCCGTCGTCTGCCTTACAGAAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGTCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTTCCGATGACATCGTTAAGATGCTCAATGATCAAGAACTTAACCGGCACACTATACAGGTTGACGCGCACGCAAGAGTTGTTTTAAATGAGGGGAACAAGAAATTTACCGACACaa              >  2:573081/1‑276 (MQ=255)                                                                                                                                                                                                                                                            cGTATGATATGGTTGCCCATACGGCAAAGTGTAGTTCAGGAAAGTAGCAATCGCAGTCCGACACCATCACTGGGCTATCGCAGTAAAAAGATAGTAATGGAACAAAAGGCTATGTGTTTGTAGGGGAATCGCTGGATGACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGc        <  4:254796/276‑1 (MQ=255)                                                                                                                                                                                                                                                             gTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATTACATCGGTAAGATGCTAAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGGTTTGTTTTAAATGCGGGGAAAAAAAAATTTAACGGAACAATACCGc        >  3:551174/1‑275 (MQ=255)                                                                                                                                                                                                                                                                 gCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCt                                                                                                                                                       >  3:653099/1‑128 (MQ=255)                                                                                                                                                                                                                                                                  ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTtac   >  3:536447/1‑275 (MQ=255)                                                                                                                                                                                                                                                                   tctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAAGGCGGGGAAAAAGAAATTTACCGGCAAAATATCGCTTtact  >  4:16464/1‑275 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   CGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACT  >  NC_000913/3875263‑3875795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑