Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,047 T→G F72V (TTT→GTT)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,0470TG98.5% 465.7 / NA 136F72V (TTT→GTT) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base G (65/66);  minor base A (0/2);  total (66/70)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 136 total aligned reads displayed.

AAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACC  >  NC_000913/2535777‑2536319
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                
     aCAATCTGCTAATCCACGAGATGCGGCCCAATTTACGGCTTAGGAGAAGATCATTGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAaacca                                                                                                                                                                                                                                                                         <  1:34458/275‑1 (MQ=255)
         tCTGGTAATCCAGGGGGTGCGGCCCAATTTACTGGTTAGGGGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCAc                                                                                                                                                                                                                                                                    <  2:76773/276‑1 (MQ=255)
            gCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGc                                                                                                                                                                                                                                                                  <  3:75279/275‑1 (MQ=255)
                   cACGAGATGCGTCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTTTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTa                                                                                                                                                                                                                                                          <  2:330968/276‑1 (MQ=255)
                     cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAGCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGAATTCTCTATc                                                                                                                                                                                                                                                        >  2:139911/1‑276 (MQ=255)
                         aTGCGGCCAAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGAGACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGaa                                                                                                                                                                                                                                                     <  1:610751/275‑1 (MQ=255)
                            cGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAAACGTTGAAACCAACCACGCATTCTCTATCGAATCTg                                                                                                                                                                                                                                                 >  2:30472/1‑276 (MQ=255)
                               cccAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTATCTATCGAATCTGAt                                                                                                                                                                                                                                               >  3:497747/1‑275 (MQ=255)
                                ccAATTTACTACTTAAGAGAAGATCATTGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTAGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTCTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                                                                                                             <  2:330676/276‑1 (MQ=255)
                                   aTTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAgcg                                                                                                                                                                                                                                           <  1:415752/275‑1 (MQ=255)
                                         tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTCTCGAATCTTATAGTGGCGt                                                                                                                                                                                                                                       >  1:480482/1‑273 (MQ=255)
                                          ggttAGGGGAAGATTACTAGTTTGTTCGATCAACTGAATTCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAGCGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGa                                                                                                                                                                                                                                    <  4:416570/273‑1 (MQ=255)
                                              aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGt                                                                                                                                                                                                                               >  2:513780/1‑276 (MQ=255)
                                                   aagagaaTGGGTTTGTTCGATAAACTGAAATCGCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCACTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGGGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTc                                                                                                                                                                                                                          <  4:303509/270‑1 (MQ=255)
                                                    aaaTCATTGGTTTGTTCGATAAGCTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAAGTATTGGGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTc                                                                                                                                                                                                                          <  1:677674/273‑1 (MQ=255)
                                                     gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTTCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATTGTTGAACCCAACAACGCCTTCTCTATCGAATCTGATAGCGGCGTTGAAATTTTCGTca                                                                                                                                                                                                                         >  3:177453/1‑274 (MQ=255)
                                                     gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTGTCGAATCTGAAAGCGGGCTTTAACTGTTcagcc                                                                                                                                                                                                                         >  3:224793/1‑271 (MQ=255)
                                                     gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATACCGGCGTTGAACTGTTCTTCCa                                                                                                                                                                                                                        >  2:162284/1‑276 (MQ=255)
                                                     gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCCCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGTGTTGCACTGTTCGttc                                                                                                                                                                                                                         >  1:173552/1‑273 (MQ=255)
                                                     gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGAACACTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAACCCAACCACGCATTCTCTATCGAATTCGACAGCGGCGTTGAATTGTTctgtc                                                                                                                                                                                                                         >  3:293147/1‑271 (MQ=255)
                                                        cATGTGTTTGTTCTATAAACTGAAATCTCTGGTTTCCGTCGACAAGAAGGATACCGGAACTATTGAGATCATTCCTCCGCTCTCTTGCGAGATCGTCAATATCGAAGACGGGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt                                                                                                                                                                                                                      <  3:292506/275‑1 (MQ=255)
                                                          tGGGTTTGTTTGTGAAACTGAAATCTCTGGTGTCCGTCGACAAGAAGGATACCGGAACTATTGAGAGCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAGTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATGCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc                                                                                                                                                                                                                    <  3:388898/275‑1 (MQ=255)
                                                          tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGCCAATATCGAAGACGTGCCGGCTGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAAAGGTGGCGCCAGTAGTAGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATATGATAGCGGCGTTTAACTGTCCGTCCACTTc                                                                                                                                                                                                                    >  1:570617/1‑275 (MQ=255)
                                                          tGGGTTTGTTACATAACCTGAAAGCTCTGGTTTCAGACGAAAAGAAGGATACCGGAACTATGGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCGATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc                                                                                                                                                                                                                    <  3:461204/275‑1 (MQ=255)
                                                                tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCgct                                                                                                                                                                                                                 >  3:35170/1‑270 (MQ=255)
                                                                tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCGATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCCAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc                                                                                                                                                                                                              >  3:120569/1‑275 (MQ=255)
                                                                    cGATAAACTGAAATCTCTGGTTCAGGACGACAAGAAGAATACATGAACTATTGAGATCATTGCTCCGCTCTCTGGCTAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGaca                                                                                                                                                                                                          <  4:503766/275‑1 (MQ=255)
                                                                       tAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACACAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGAAACCACGGTTGAACTTTTCCGCCACCTCgtttctgccaccgt                                                                                                                                                                                                      >  4:58188/1‑263 (MQ=255)
                                                                            tGAAATCTTTGGTTTACGACGAAAAGAACGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCTTCAAGATCGAAGATGTTCCGGATGTCGTTTGTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGGCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGTCGTTGAACTGTTCGTCCACTTCGGTATCTACACCGTTGaa                                                                                                                                                                                                  <  2:624045/275‑1 (MQ=255)
                                                                                 tctcTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTACCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGcaa                                                                                                                                                                                            >  2:432409/1‑276 (MQ=255)
                                                                                                 acAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTTAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTCTCGACACCGTTGAACTGAAAGGCGGAGGCttgcagc                                                                                                                                                                            >  4:217552/1‑271 (MQ=255)
                                                                                                   aagaagAATACAGGAACTATTTATATCATTGCTCCGCTCTCTGGCAAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGt                                                                                                                                                                          <  2:224524/276‑1 (MQ=255)
                                                                                                    agaagGATACCCGAACACTTTAGATCCTTTCTCCGCTATCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGCTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTa                                                                                                                                                                         <  4:432409/276‑1 (MQ=255)
                                                                                                      aagTATACCGGAAATTTTGAGATCAGTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTa                                                                                                                                                                         <  1:150955/274‑1 (MQ=255)
                                                                                                      aaagATACCGGAACTATTGGGATCATTGCTCCGCTCTCTGGCGAAATCGTCAATATCGAAGACGTGACGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATGGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAt                                                                                                                                                                        <  3:9042/272‑1 (MQ=255)
                                                                                                                   cTATTGAGATCATTTCTCCGCTCTCTGGCGAGCTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGCAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTTAACTGATAGGCGAAGGCTTCAAGCTTATTCCTGAAGACGGTc                                                                                                                                                          >  2:519146/1‑276 (MQ=255)
                                                                                                                   cTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGCTAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGACGGCTTCAAGCGTATTGCGTAAGAAGGTc                                                                                                                                                          >  2:635417/1‑276 (MQ=255)
                                                                                                                      ttGAGATCATTGCTCCGCTACTTGGCGGGAACGTCAATAGCGAAGACGTGCCGGATGTCTGTTTTGCCGAAAAAAGCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCCTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAg                                                                                                                                                        <  3:161592/275‑1 (MQ=255)
                                                                                                                        gagaGCATTGGTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAg                                                                                                                                                        <  3:49268/273‑1 (MQ=255)
                                                                                                                           aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCCTTCTCTATCCAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTCCACGCGTCTTGCCGAAGAACGTCAGcgcgc                                                                                                                                                   >  3:387457/1‑274 (MQ=255)
                                                                                                                           aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATAAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGAGCCATTGGGAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTACGGTATCGACACCGTTGAACTGACAGGCGAAGGCTTACAGCGTATTGCTGAAGAAGTTCAGCGCGt                                                                                                                                                   >  2:416807/1‑275 (MQ=255)
                                                                                                                             cATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTGTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTGTCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGa                                                                                                                                                 <  1:224793/275‑1 (MQ=255)
                                                                                                                              aTTGCTCCGCTCTCTGGCGAGATCGTCAATGTCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCCCCAGTAGACGGCACCATTGGTAAACTCGTAGAAACCCACCACGCATTACCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTACACTTCGGTATACACACCGTTACAATGAAAGGCGAAGGCTTCAAGCGTCTTTCTGACAAAGGTCAGCGCGt                                                                                                                                                   >  4:241430/1‑272 (MQ=255)
                                                                                                                              aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACAATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATACAATCTGATAGCGGCGTTGAAATGTTCGTCCACTTCGGTATCGACCTCGGTGAACTGAAAGGCGCAGGCTTACAGCGTCATGCTGAAGAACGTGAgccaggtgaa                                                                                                                                               >  4:674571/1‑268 (MQ=255)
                                                                                                                                tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGTGTTGAACTGTTCTTCCACTTCGGTATCGACACCGTCGAACTGAAAGGCTAAGGCTTTAAGCGTATTTCTAATGACGGTCAGCTCTTGAAAg                                                                                                                                              >  1:526349/1‑275 (MQ=255)
                                                                                                                                tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGAATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTTCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                                                                                                                                              >  1:64888/1‑275 (MQ=255)
                                                                                                                                 gctcagccatctGGCGAAATCGTGAATATCGAAGACGTGCCTGATGTCGTTTTTTCTGAAAAAATGGTTTGTGGTGGTATTGCTATCAAACCAACTGGTATCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGTCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGt                                                                                                                                             <  3:369403/266‑1 (MQ=255)
                                                                                                                                     cGCTCCCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACTGTTGAACTGAAAGGCGAAGGCTTACAGCGTATTGCTGAATACGGTCAGCCCGGGAACGTTGGc                                                                                                                                         >  3:410739/1‑275 (MQ=255)
                                                                                                                                        tctgtGGCGAGATCGGCAATTTCGAAGACGTGCCGGAAGTCATTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAAACGGCACCAGTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                                                                                                       <  3:173552/274‑1 (MQ=255)
                                                                                                                                        tctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTGTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                                                                                                       <  1:564649/274‑1 (MQ=255)
                                                                                                                                         ctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCTTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                                                                                                     <  2:24869/275‑1 (MQ=255)
                                                                                                                                              gCGAGATCGTAAATATCGAAGACGGGCCGGATGTCGGTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCa                                                                                                                               <  2:217552/276‑1 (MQ=255)
                                                                                                                                               cgtgctCGTCAATATCGAAAACGTGCCGAATGTCGTTTTAGGGGAAAAAATCGTTGGTGATGGTATTGATATCTAACCAACGGGTAACAGAATAGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCa                                                                                                                               <  3:526349/270‑1 (MQ=255)
                                                                                                                                                ggggacGTCAATATCGAAGTCTGGCCCGATGTGATTTTGGCGGAAAACATCGTTGGCGAGGTTATGGCGATCAAACCAACGGGTAACAGAAGGGTCGCGCCAGTATACGGCACCAGTGGTGAAATCGTTGATACCAACCACGCAATCTCTATCGGATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACAACGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCGGAAGAAGGTCAGCGCTTGAAAGTTGGCGATACTGTCa                                                                                                                               <  3:175712/269‑1 (MQ=255)
                                                                                                                                                 agaTCGTCAATATCGAAACCGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAt                                                                                                                              <  1:374717/274‑1 (MQ=255)
                                                                                                                                                    tCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGACCTGAAAGGCGACGGCTTCAAGCGTATTGCTGAAGAAAGTCAGCGCGTGAAGATTGGCCATAcgttcctttaat                                                                                                                         >  4:679341/1‑265 (MQ=255)
                                                                                                                                                      gTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAtt                                                                                                                        <  1:722278/275‑1 (MQ=255)
                                                                                                                                                        cAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGAGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGAGTGAAAGTTGGCGATACTGTCATTGAATTTg                                                                                                                      >  1:477892/1‑275 (MQ=255)
                                                                                                                                                                aaGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCg                                                                                                              >  3:515819/1‑275 (MQ=255)
                                                                                                                                                                      taccggttggtttttttACGAAAAAAATTGTTGGTTCTGGTAGTGCTCTCAAATCAACGGTGAACAAAATGACCGCGCCATTAGACGGCACCATTGGTCAATTCGTTGCACCCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCTACTTCTGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAATAAGGTCTGCGCTTGAATGTTGGCGATACTTTCATTGAATTTGATCTGCCGCTGCTgg                                                                                                       <  4:28189/264‑1 (MQ=255)
                                                                                                                                                                               acggttttGCGGAAAAAATCGTTGATGATGTGATTGCTATCAAACCAACAGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAgaga                                                                                                 <  3:64888/269‑1 (MQ=255)
                                                                                                                                                                                 gTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGCTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                             <  2:108402/275‑1 (MQ=255)
                                                                                                                                                                                 gTTTTAGCGGAAAAAAAAGTAGGTGATGGTATTGCTTTCAAACCAACGAGGAACAAAAGGGTCGGGCCAGTAGACGGGACCATTGGTAAAATCATGGAAACCAACCACGCATGCACTATCGGATCTGATAGCGGCGTTGAACTGTTCGTCAACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                             <  4:578787/275‑1 (MQ=255)
                                                                                                                                                                                    tttGCGGAGAAAAGCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                             <  4:49190/272‑1 (MQ=255)
                                                                                                                                                                                      tGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGCATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTTCTGGAACAGAAAGACAAGt                                                                                        >  3:42855/1‑275 (MQ=255)
                                                                                                                                                                                           aaaaaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTAGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTAcc                                                                                   <  1:42855/275‑1 (MQ=255)
                                                                                                                                                                                             aaaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCt                                                                                 <  3:321975/275‑1 (MQ=255)
                                                                                                                                                                                                    ttGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTAAGCGCGTGAAAGTTGGCGATACGGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCGAACTCCg                                                                          >  4:523349/1‑275 (MQ=255)
                                                                                                                                                                                                     tGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTTTCTACCGAATCTGATAGCGGCGTTGAACTGTTCGTCCTCTTCGGTATCGACACCGTTGAACTGAACGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTCGCGATACTGCCATTGATTCTGCTTTTCCGCCGCCGGAACAGTAAGCAAAGTCTACCTTGACTCCgt                                                                         >  3:233750/1‑274 (MQ=255)
                                                                                                                                                                                                      ggTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGctgctg                                                                                                        >  1:497896/1‑243 (MQ=255)
                                                                                                                                                                                                        tGATGGTAGTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGGTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTTGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt                                                                     <  2:58188/276‑1 (MQ=255)
                                                                                                                                                                                                         gaaggTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt                                                                    <  2:572389/273‑1 (MQ=255)
                                                                                                                                                                                                               aTTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATGGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAtctc                                                               <  3:251283/275‑1 (MQ=255)
                                                                                                                                                                                                                      tCAAACCAACGGGTAACACAATGGTCGCGACAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGACTCTGATAGCGACGTTGAACTGTAAGTCCACTTCGGTATCCACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATAATGTCATTGAATTTGATATGCCGCTGCTGGAAGAGACAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCCACATgg                                                       >  4:546405/1‑276 (MQ=255)
                                                                                                                                                                                                                      tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATgg                                                       >  2:157454/1‑276 (MQ=255)
                                                                                                                                                                                                                       cAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATgg                                                       >  2:60950/1‑275 (MQ=255)
                                                                                                                                                                                                                        aaaCCAACGTGTAAAAAAATGTTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAGCTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGa                                                      <  4:513780/275‑1 (MQ=255)
                                                                                                                                                                                                                        aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTCCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGCGAAAGTTGGCGATACGGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGCCTACCCTGACTCCGGTTGTTATATCCAACATgg                                                       >  2:256216/1‑274 (MQ=255)
                                                                                                                                                                                                                           ggacagggTAACAAAATGGTCGCGTCAGTAGACGGGACAATTGGTAAAATCGTTTAAACCAACCAAGCATTTTCTATCGAAACTGAAAGCAGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGa                                                   <  3:560373/270‑1 (MQ=255)
                                                                                                                                                                                                                                gggTAACAAAATGGTCTCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCa                                              <  1:387457/275‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTTTCGAATCTGATAGCGGCGTTGAACTGTTCGGCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                          <  1:576576/275‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCAAAATGGGCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                          <  1:166608/275‑1 (MQ=255)
                                                                                                                                                                                                                                     aCAAAATGGTCGCGCCAGTAGACAGCACCATGGGTAAAATCAGTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGGTGAACTGTTCGTCCACTGCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAAc                                        <  4:519146/276‑1 (MQ=255)
                                                                                                                                                                                                                                           tGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGCAATCAAAGAACTGaac                                   >  2:362318/1‑273 (MQ=255)
                                                                                                                                                                                                                                             gTCGGGCCAGTAAACGGCACCATTGGTAAATTCGTTTAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                <  4:224097/276‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTg                             >  2:509484/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 cgcCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACACATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGCAATCAAAGAACTGATCACACt                              >  1:599279/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            ggCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCTTTGCACTGTTCGTCCACTTCGGCATCCACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAACTTGATCTGCCGCTGCTGGAAGAGAAAGCCAATTCTACCCTGATCCCGGTTGTTATCTCTCACATGGACGAAAGCAAAGAACTGATCAAACTGTCCGGTAGCg                   >  1:107696/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            ggCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAACGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGt                  >  2:143736/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            ggCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCGATCGAAACGGATAGCGGCGTTGAACTGTTCGTCCACTGCGGTATCGACACCGTTGAAATGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTGGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCg                   <  1:410739/274‑1 (MQ=255)
                                                                                                                                                                                                                                                              cACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTa                 >  1:121727/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                ccATTGGTAAGATCGTTGAAACCAACCACGGATTCTCTGTCGAATCAGATAGCGGCGTTGAACTGTTCGTCCACGTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAAc               <  1:305152/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTTTCCGGTAGCGTAAccct            >  2:29457/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                 cATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGGTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGAGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCGGGAAGAGAAAGCCAAGTTTACTCTGACTCCGGTTGTTATCTCTAACATGGACGAAAACAAAGAACCGATCAAACTGTCCGGTAGCGTAAcag             >  1:310073/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                  atttggaaaaCCGTTGGAACCAACCAAGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCCGCCACTTCGGTAACGACACCGTTGAACTGAAAGGCGAAGGTTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGCTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGt            <  1:293147/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aTTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCg             <  1:515819/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCCAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAATAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGGTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCCCCAACATGGACGAAATCAAAGAACAGATCAAACTGTCCGGTAGCGTAACCGttg          >  2:414867/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                        aaaaTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGa      >  3:421691/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           aTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGa                                                      >  4:620174/1‑224 (MQ=255)
                                                                                                                                                                                                                                                                           aTCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTGGTTATCTCCAACATGGACGAAATCAACGAACTGATCAAACTGTCAGGTAGCGTAACCGTTGGTGAAAcc  >  4:304328/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                
AAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACC  >  NC_000913/2535777‑2536319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: