Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,847:1 +C 5.9% intergenic (+13/+17) yhjV → / ← dppF putative transporter/dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8471.C 5.9% 959.2 / 55.8 219intergenic (+13/+17)yhjV/dppFputative transporter/dipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base . (86/120);  new base C (4/9);  total (90/129)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

Only 100 of 222 total aligned reads displayed.

TGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAAC  >  NC_000913/3701699‑3701994
                                                                                                                                                     |                                                                                                                                                   
 gtgtCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATgg                                                                                                                                                    <  1:1180117/149‑1 (MQ=255)
 gtgtCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATgg                                                                                                                                                    <  1:439370/149‑1 (MQ=255)
  tgtCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGc                                                                                                                                                   <  2:727340/149‑1 (MQ=255)
    tCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGccc                                                                                                                                                 >  4:1005929/1‑149 (MQ=255)
    tCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGccc                                                                                                                                                 >  2:146741/1‑149 (MQ=255)
         ccTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtt                                                                                                                                            <  2:613284/149‑1 (MQ=255)
          cTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGttt                                                                                                                                           <  2:1374425/149‑1 (MQ=255)
           tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGttta                                                                                                                                          <  3:332470/149‑2 (MQ=255)
           tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                          >  4:878772/1‑149 (MQ=255)
           tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                          <  4:578258/149‑1 (MQ=255)
           tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                          <  4:1015813/149‑1 (MQ=255)
             aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttac                                                                                                                                         <  4:1666734/148‑3 (MQ=255)
              ttCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGGTTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGTGCGCATGGGCCCGGCTTtgtg                                                                                                                                       >  3:1222863/1‑149 (MQ=255)
               tCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTTTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      <  2:1324684/149‑1 (MQ=255)
               tCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      <  3:1005929/149‑1 (MQ=255)
               tCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      <  3:585133/149‑1 (MQ=255)
               tCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      >  4:1554679/1‑149 (MQ=255)
                          tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCCCTGTTGAATCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                           <  2:204590/149‑1 (MQ=255)
                          tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                        <  1:516342/136‑1 (MQ=255)
                          tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                           >  2:493586/1‑149 (MQ=255)
                          tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                           <  1:683548/149‑1 (MQ=255)
                               tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTACAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTACCCCGGAGCTCATGGCCCCGGTTTTGTGAGTTAGCGCTGCGGAtt                                                                                                                      <  3:249914/149‑1 (MQ=255)
                               tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      >  2:886525/1‑133 (MQ=255)
                               tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAc                                                                                                                                <  2:939128/139‑1 (MQ=255)
                               tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtt                                                                                                                      >  3:1580593/1‑149 (MQ=255)
                               tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtt                                                                                                                      <  3:1236371/149‑1 (MQ=255)
                                ataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTGACGCTGCGGAttt                                                                                                                     >  3:64474/1‑149 (MQ=255)
                                ataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                     <  1:1110793/149‑1 (MQ=255)
                                 taAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtttt                                                                                                                    <  1:661707/149‑1 (MQ=255)
                                 taAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtttt                                                                                                                    >  4:304418/1‑149 (MQ=255)
                                     gTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                                >  1:289280/1‑149 (MQ=255)
                                     gTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                                <  2:807966/149‑1 (MQ=255)
                                     gTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                                >  1:1583197/1‑149 (MQ=255)
                                      tCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                        >  1:814828/1‑124 (MQ=255)
                                      tCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                        <  2:814828/124‑1 (MQ=255)
                                      tCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATcc                                                                                                               >  3:1618141/1‑149 (MQ=255)
                                           caACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                                                          <  3:725676/149‑1 (MQ=255)
                                              cTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                                <  1:734140/140‑1 (MQ=255)
                                              cTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAAc                                                                                                       <  2:1714498/149‑1 (MQ=255)
                                               tGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACa                                                                                                      <  3:878772/149‑1 (MQ=255)
                                                ggAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCCGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAg                                                                                                     <  4:1271840/149‑1 (MQ=255)
                                                 gAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCTCTGTTGAAGCTGATTGAGTAAACCGTAGCGCATGGCCGCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCTTGATCAACAGc                                                                                                    <  2:337325/149‑1 (MQ=255)
                                                 gAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGc                                                                                                    >  4:785619/1‑149 (MQ=255)
                                                  aaaaaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCa                                                                                                   <  3:1710164/149‑1 (MQ=255)
                                                       cTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACa                                                                                              <  1:146741/149‑1 (MQ=255)
                                                        ttCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                                                             <  1:1211850/149‑1 (MQ=255)
                                                        ttCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                                                             <  4:79196/149‑1 (MQ=255)
                                                        ttCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                                                             <  1:865068/149‑1 (MQ=255)
                                                        ttCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                                                             <  2:543066/149‑1 (MQ=255)
                                                         tCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAg                                                                                            <  4:1132405/149‑1 (MQ=255)
                                                          cgcgGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTAGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                                                           <  1:473022/149‑1 (MQ=255)
                                                          cgcaGATTTAAAGCCTGGCTGATTCCGGTGTACGGCATTTTGCTATGCTTGTCGCCATTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCTACGGTTTTGTGAGTTAACGCTGCGGATTTTCAGCCTGATCAACAGCAAACCaaac                                                                                           <  2:120213/149‑3 (MQ=255)
                                                           gcgGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                                                          >  1:276308/1‑149 (MQ=255)
                                                                         tGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgcc                                                                            >  1:1351439/1‑149 (MQ=255)
                                                                            cTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                                                         <  4:528107/149‑1 (MQ=255)
                                                                                ttCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                           <  1:936401/111‑1 (MQ=255)
                                                                                  ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCttt                                                                   <  3:969058/149‑1 (MQ=255)
                                                                                    gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                                                 <  4:1457636/149‑1 (MQ=255)
                                                                                          cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTc                                                                      <  3:789568/138‑1 (MQ=255)
                                                                                          cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                           >  4:1585980/1‑149 (MQ=255)
                                                                                               ttttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                      >  4:348908/1‑149 (MQ=255)
                                                                                               ttttGCTATGCTTGTCGCCACTGTTGAACCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                      >  4:417618/1‑149 (MQ=255)
                                                                                                tttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                     >  3:561811/1‑85 (MQ=255)
                                                                                                  tGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAAc                                                   <  1:1624521/149‑1 (MQ=255)
                                                                                                     tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAc                                                                                                                                <  1:320483/69‑1 (MQ=255)
                                                                                                     tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTgg                                                <  2:1360635/149‑1 (MQ=255)
                                                                                                     tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTgg                                                >  3:1150891/1‑149 (MQ=255)
                                                                                                     tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTgg                                                <  3:832252/149‑1 (MQ=255)
                                                                                                     tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTgg                                                >  2:689366/1‑149 (MQ=255)
                                                                                                          ttGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaaa                                                                                                <  1:955368/96‑1 (MQ=255)
                                                                                                               gCCACTGTTGAAGCTGCTTGAGTAAACCGGAGCGCATGGCCCCGGTATTGTGAGTTAACGCTGCTGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCGACTGGGTGCAGGGGc                                      <  4:1030108/149‑1 (MQ=255)
                                                                                                                ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGcc                                     >  4:1304534/1‑149 (MQ=255)
                                                                                                                 cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCg                                    >  3:783543/1‑149 (MQ=255)
                                                                                                                 cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCg                                    >  3:307096/1‑149 (MQ=255)
                                                                                                                  aCTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                                                        <  3:1537589/96‑1 (MQ=255)
                                                                                                                      ttGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                      >  3:1357185/1‑46 (MQ=255)
                                                                                                                      ttGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcg                               >  2:431625/1‑149 (MQ=255)
                                                                                                                      ttGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcg                               >  4:1153630/1‑149 (MQ=255)
                                                                                                                      ttGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcg                               >  3:1408071/1‑149 (MQ=255)
                                                                                                                       tGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcgc                              <  2:1642708/149‑1 (MQ=255)
                                                                                                                        gAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGcc                             >  2:184914/1‑149 (MQ=255)
                                                                                                                        gAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGcc                             <  2:1116445/149‑1 (MQ=255)
                                                                                                                           gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                          >  3:457614/1‑149 (MQ=255)
                                                                                                                           gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                          <  2:1621134/149‑1 (MQ=255)
                                                                                                                             tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                                                      >  1:1695780/1‑87 (MQ=255)
                                                                                                                             tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgacga                        >  2:95624/1‑149 (MQ=255)
                                                                                                                                 ttgatAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGc                    <  1:96906/145‑1 (MQ=255)
                                                                                                                                 tcacaAAACCGGGGCCATGCGCGCCGGTGGTGTGAGTTAACGCTGCGGAGTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGc                    <  1:393802‑M1/144‑1 (MQ=255)
                                                                                                                                   acaAAACCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGtt                                                                                   >  3:25757‑M1/4‑84 (MQ=255)
                                                                                                                                   acaAAACCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtt                                                                    >  3:276228‑M1/4‑99 (MQ=255)
                                                                                                                                   aGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCgg                  <  1:1262993/149‑1 (MQ=255)
                                                                                                                                    gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCggg                 >  4:845640/1‑149 (MQ=255)
                                                                                                                                     tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCt                                                              >  2:1590987/1‑103 (MQ=255)
                                                                                                                                     tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCt                                                              <  1:1590987/103‑1 (MQ=255)
                                                                                                                                     tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGc                >  1:1040749/1‑149 (MQ=255)
                                                                                                                                           GGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGttgga          >  2:1525801‑M1/1‑147 (MQ=255)
                                                                                                                                            GAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAg         >  2:276542‑M1/1‑149 (MQ=255)
                                                                                                                                                 cATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAggg                                        >  4:1297637/1‑113 (MQ=255)
                                                                                                                                                  aTGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCaa   >  3:1271703/1‑149 (MQ=255)
                                                                                                                                                   tGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAAc  <  1:184914/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAAC  >  NC_000913/3701699‑3701994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: