Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,213,187:1 +A 5.8% intergenic (+28/‑47) rrfE → / → yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,1871.A 5.8% 970.6 / 51.3 226intergenic (+28/‑47)rrfE/yjaA5S ribosomal RNA of rrnE operon/stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base . (100/113);  new base A (6/7);  total (106/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

CTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAAC  >  NC_000913/4213043‑4213329
                                                                                                                                                 |                                                                                                                                              
cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                                                                            >  4:1353146/1‑149 (MQ=255)
cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                                                                            <  2:675149/149‑1 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCTATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                                           <  2:144197/149‑1 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATACGGCCggg                                                                                                                                           <  4:881443/149‑1 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                                           <  2:405988/149‑1 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                                           >  3:478048/1‑149 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                                           <  3:45596/149‑1 (MQ=255)
 tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                                           <  4:74587/149‑1 (MQ=255)
  ggcggcCGTAGCGCAGTGGATCCACCTGACCACCTGCCGAACTCAGATGTGTAACGCCGTCGCGCAGAAGGTAGTGTGGGGTCTCCCCATGCGAGACTAGGGAACTGCCAGTAATCAAATTAGAAAAACCCCGATCCATAAGGCCgggg                                                                                                                                          >  4:751160/1‑149 (MQ=14)
     ggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGCCTTATGGACCGGGGttt                                                                                                                                       <  4:1107693/149‑1 (MQ=14)
        aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttt                                                                                                                                    >  3:701723/1‑149 (MQ=255)
        aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAAGTAGAAAAACCCCGGCCTTATGGACCGGGGtttttc                                                                                                                                    <  3:807031/149‑2 (MQ=14)
         gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGCCTTATGGACCGGGGtttttct                                                                                                                                   <  1:1625342/149‑3 (MQ=14)
           aGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa                                                                                                                                 <  4:1044225/149‑1 (MQ=255)
            gcgcGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCat                                                                                                                                >  2:1290189/1‑149 (MQ=255)
                ggtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCa                                                                                                                            >  2:499046/1‑149 (MQ=255)
                   ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAatt                                                                                                                         <  2:1076286/149‑1 (MQ=255)
                       ccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTAttt                                                                                                                     >  4:1501235/1‑149 (MQ=255)
                         aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGc                                                                                                                   >  1:185099/1‑149 (MQ=255)
                           cTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCat                                                                                                                 <  1:1648182/149‑1 (MQ=255)
                             gACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatga                                                                                                               >  3:1634444/1‑149 (MQ=255)
                                cccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCAtgatga                                                                                                            >  3:1463596/1‑149 (MQ=255)
                                 ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAat                                                                                                                          <  2:1216013/134‑1 (MQ=255)
                                 ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTAttt                                                                                                                     >  4:705414/1‑139 (MQ=255)
                                 ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGaa                                                                                                           <  3:529504/149‑1 (MQ=255)
                                 ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGaa                                                                                                           <  1:1013943/149‑1 (MQ=255)
                                  cATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTCTTGCATATCAATTATTTGCATGATGAAg                                                                                                          <  3:1326706/149‑1 (MQ=255)
                                   aTGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAgg                                                                                                         <  2:1498693/149‑1 (MQ=255)
                                    tGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCCTGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAggg                                                                                                        <  4:1689855/149‑1 (MQ=255)
                                       cGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAt                                                                                                     <  2:177855/149‑1 (MQ=255)
                                            tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                                                >  2:855846/1‑149 (MQ=255)
                                            tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                                                >  3:123054/1‑149 (MQ=255)
                                              aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa                                                                                                                                 <  1:1579126/114‑1 (MQ=255)
                                              aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                                                                              >  2:1629691/1‑149 (MQ=255)
                                              aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                                                                              >  1:1545526/1‑149 (MQ=255)
                                                aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCa                                                                                            >  1:668407/1‑149 (MQ=255)
                                                  gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatgat                                                                                                              >  1:1638980/1‑129 (MQ=255)
                                                     aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                                                        <  1:755070/148‑1 (MQ=255)
                                                     aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                                                        >  2:1106445/1‑148 (MQ=255)
                                                     aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                                                        <  1:1106445/148‑1 (MQ=255)
                                                     aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTc                                                                                        >  2:755070/1‑148 (MQ=255)
                                                      aaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttt                                                                                                                                       >  4:1614698/1‑100 (MQ=255)
                                                      aaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTg                                                                                      >  4:279582/1‑149 (MQ=255)
                                                                      tGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                      <  2:1004649/149‑1 (MQ=255)
                                                                      tGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                      <  2:1682677/149‑1 (MQ=255)
                                                                      tGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                      <  4:1579690/149‑1 (MQ=255)
                                                                                gggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAt                                                            <  3:1551851/149‑1 (MQ=255)
                                                                                gggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAt                                                            >  3:1363810/1‑149 (MQ=255)
                                                                                    ctcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACt                                                        <  4:478048/149‑1 (MQ=255)
                                                                                       cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGtt                                                     >  4:34528/1‑149 (MQ=255)
                                                                                       cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGtt                                                     >  4:910540/1‑149 (MQ=255)
                                                                                       cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGtt                                                     >  1:264250/1‑149 (MQ=255)
                                                                                        ccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAATACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTc                                                    <  4:681098/149‑1 (MQ=255)
                                                                                        ccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTc                                                    <  1:1684810/149‑1 (MQ=255)
                                                                                        ccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATCACTGTTc                                                    <  4:1018622/149‑1 (MQ=255)
                                                                                            gCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGTGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCACGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGa                                                <  4:1266060/149‑1 (MQ=255)
                                                                                               agagTAGGGAACTGCCCGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGTGGTTTTTTGCATATCCATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAGTACTGTACGCGATCt                                             <  2:370321/149‑1 (MQ=255)
                                                                                               agagTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCt                                             <  3:1501235/149‑1 (MQ=255)
                                                                                                  gTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGa                                          <  2:264250/149‑1 (MQ=255)
                                                                                                   tAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa                                         >  1:729374/1‑149 (MQ=255)
                                                                                                    aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                                        <  3:1086972/149‑1 (MQ=255)
                                                                                                    aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                                        <  1:859623/149‑1 (MQ=255)
                                                                                                    aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                                        >  2:868738/1‑149 (MQ=255)
                                                                                                    aGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                                        <  1:1290189/149‑1 (MQ=255)
                                                                                                     gggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAg                                       >  2:1639209/1‑149 (MQ=255)
                                                                                                      ggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGc                                      >  4:1147136/1‑149 (MQ=255)
                                                                                                      ggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGc                                      <  3:579014/149‑1 (MQ=255)
                                                                                                      ggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGc                                      >  1:1230249/1‑149 (MQ=255)
                                                                                                         aCTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCaaa                                   <  3:664321/149‑1 (MQ=255)
                                                                                                          cTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTACTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAAc                                  <  4:826461/149‑1 (MQ=255)
                                                                                                             ccAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAAc                                  >  3:283335/1‑146 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             <  3:242472/149‑1 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             >  3:623707/1‑149 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             >  1:538029/1‑149 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             <  3:796818/149‑1 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             <  1:139720/149‑1 (MQ=255)
                                                                                                               aGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa                             >  2:563425/1‑149 (MQ=255)
                                                                                                                 gCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgt                           <  1:1220018/149‑1 (MQ=255)
                                                                                                                  cATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtg                          >  1:816007/1‑149 (MQ=255)
                                                                                                                  cATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtg                          >  1:608187/1‑149 (MQ=255)
                                                                                                                   aTCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtgt                         >  1:1407084/1‑149 (MQ=255)
                                                                                                                    tCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTc                        <  3:1181231/149‑1 (MQ=255)
                                                                                                                    tCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTc                        >  4:1715444/1‑149 (MQ=255)
                                                                                                                        aTTAGAAAAACCCCGGACCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGga                    >  4:1177915/1‑149 (MQ=255)
                                                                                                                          tAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGgaga                  <  3:885539/149‑1 (MQ=255)
                                                                                                                           aGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGAc                 <  4:1529116/149‑1 (MQ=255)
                                                                                                                           aGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAACTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGAc                 >  3:1609982/1‑149 (MQ=255)
                                                                                                                            gAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAg                      <  1:964470/143‑1 (MQ=255)
                                                                                                                            gAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAg                      >  2:964470/1‑143 (MQ=255)
                                                                                                                             aaaacCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATCTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGCCGc               <  2:1703793/149‑1 (MQ=255)
                                                                                                                             aaaaaCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGc               >  2:629009/1‑149 (MQ=255)
                                                                                                                             aaaaaCCCCGGCCTTATGGACCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGAc                 >  3:116540/1‑147 (MQ=255)
                                                                                                                               aaaCCTCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTg             >  4:1426389/1‑149 (MQ=255)
                                                                                                                                 aCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                                                                          <  3:1539951/54‑1 (MQ=255)
                                                                                                                                   cccGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAAt                                                                 >  1:713977/1‑93 (MQ=255)
                                                                                                                                   cccGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGATGCTGCCtt         >  2:1035569/1‑149 (MQ=255)
                                                                                                                                   cccGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCtt         >  1:465721/1‑149 (MQ=255)
                                                                                                                                      ggTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAg      <  4:1182519/149‑1 (MQ=255)
                                                                                                                                             aaGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGTGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                      <  2:1317337/78‑1 (MQ=255)
                                                                                                                                             aaGGCCGGGGTTTTGTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                                      >  1:1317337/1‑78 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAAC  >  NC_000913/4213043‑4213329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: