Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,285,870:1 | +G | 7.3% | coding (1502/1959 nt) | acs ← | acetyl‑CoA synthetase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,285,870 | 1 | . | G | 7.3% | 803.5 / 61.1 | 191 | coding (1502/1959 nt) | acs | acetyl‑CoA synthetase |
Reads supporting (aligned to +/- strand): ref base . (85/92); new base G (5/9); total (90/101) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
Only 100 of 197 total aligned reads displayed.
ATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTC > NC_000913/4285723‑4286018 | aCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGt > 3:1604909/1‑149 (MQ=255) ccTACTACGGCGGCATCGGCAATCTTCGGATGGGCCACGAGCGCCGAATCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGA‑CGTc > 2:193678/1‑149 (MQ=255) ctactaCGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTcg > 4:926520/1‑149 (MQ=255) actacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTcgcc > 1:527166/1‑149 (MQ=255) ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga < 4:799525/149‑3 (MQ=255) ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga < 1:415598/149‑3 (MQ=255) tacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat < 1:919376/149‑4 (MQ=255) ggcggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGa < 2:58810/149‑1 (MQ=255) cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGaaa < 1:16900/149‑1 (MQ=255) cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGaaa < 3:939682/149‑1 (MQ=255) gcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATa < 3:317951/149‑1 (MQ=255) cTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATAc > 1:708088/1‑149 (MQ=255) tCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCTAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACat < 2:1059917/149‑1 (MQ=255) gCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATAtt > 3:9969/1‑149 (MQ=255) ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGaa > 2:370572/1‑149 (MQ=255) ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt < 1:1481659/149‑1 (MQ=255) gATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGaaa > 3:459934/1‑131 (MQ=255) gATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg < 2:468641/149‑1 (MQ=255) gATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg < 2:13618/149‑1 (MQ=255) gcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa > 3:800367/1‑149 (MQ=255) ccaccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat > 4:802097/1‑117 (MQ=255) caccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt < 2:1597012/149‑1 (MQ=255) caGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAg < 2:540863/136‑1 (MQ=255) caGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat > 1:250419/1‑113 (MQ=255) caGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat < 2:250419/116‑4 (MQ=255) aGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGt < 3:1641951/149‑1 (MQ=255) aGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGt < 4:5838/149‑1 (MQ=255) cGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt < 3:1502559/149‑1 (MQ=255) gACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTc < 2:527166/149‑1 (MQ=255) cTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCt > 2:946053/1‑143 (MQ=255) tCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACatat < 1:390702/118‑1 (MQ=255) tCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaaa > 3:90073/1‑149 (MQ=255) cAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaaaa < 2:324696/149‑1 (MQ=255) cAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaaaa < 1:1447282/149‑1 (MQ=255) aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc > 1:301729/1‑149 (MQ=255) aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACg > 1:180083/1‑149 (MQ=255) tctcTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga > 2:1694368/1‑99 (MQ=255) tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc < 1:1128175/149‑1 (MQ=255) tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc < 1:226788/149‑1 (MQ=255) tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc < 1:981956/149‑1 (MQ=255) tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt < 1:1615860/149‑1 (MQ=255) cccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt < 1:450536/142‑1 (MQ=255) cccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt > 2:450536/1‑142 (MQ=255) cccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGcc > 2:751979/1‑149 (MQ=255) ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa > 3:969141/1‑149 (MQ=255) ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa > 3:139544/1‑149 (MQ=255) ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc < 1:1062857/149‑1 (MQ=255) ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc < 3:282337/149‑1 (MQ=255) aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCt > 4:984765/1‑116 (MQ=255) aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCt < 3:984765/116‑1 (MQ=255) gagaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc < 2:183437/149‑1 (MQ=255) gagaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc > 1:508590/1‑149 (MQ=255) gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCt < 3:835909/149‑1 (MQ=255) gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAc > 4:668015/1‑149 (MQ=255) gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat < 1:814157/71‑4 (MQ=38) gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcgat > 2:814157/1‑68 (MQ=38) gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga < 1:814952/70‑3 (MQ=255) gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga > 2:814952/1‑68 (MQ=255) ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGga > 1:542045/1‑95 (MQ=255) cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGCAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc > 3:199803/1‑149 (MQ=255) cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc < 3:1678338/149‑1 (MQ=255) gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt > 4:213209/1‑149 (MQ=255) gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt < 3:598558/149‑1 (MQ=255) tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc > 2:1646929/1‑149 (MQ=255) tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc > 2:1336370/1‑149 (MQ=255) cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg > 2:998276/1‑149 (MQ=255) cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg > 2:140130/1‑149 (MQ=255) cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg > 1:579861/1‑149 (MQ=255) acacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt < 4:1637024/149‑1 (MQ=255) acacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt < 4:1380933/149‑1 (MQ=255) aaacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt > 1:1393797/3‑149 (MQ=255) cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg < 4:1500710/149‑1 (MQ=255) acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa < 1:650268/81‑1 (MQ=255) acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa > 2:650268/1‑81 (MQ=255) gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt < 3:1199441/149‑1 (MQ=255) ccGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc > 2:1179476/1‑149 (MQ=255) cGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAcc > 1:861089/1‑149 (MQ=255) ggTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt > 2:1468044/1‑98 (MQ=255) ggTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCa < 3:681429/149‑1 (MQ=255) gctATCCAGTAATAGTCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg < 2:509993/147‑1 (MQ=255) gTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg < 4:1584633/149‑1 (MQ=255) aTCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCt > 2:39041/1‑149 (MQ=255) ccAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAc < 1:1179476/149‑1 (MQ=255) cAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaaa < 4:1225059/83‑1 (MQ=255) cAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc < 1:345945/149‑1 (MQ=255) aGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCt > 4:750548/1‑149 (MQ=255) tAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg < 1:1364988/140‑1 (MQ=255) tAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg > 2:1364988/1‑140 (MQ=255) tAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTc > 3:864816/1‑149 (MQ=255) ctgCCATCTTCATCGCGAC‑GCTCGC‑CGTCGCCGCTGAAATAGATATTTTTGAAGGTGGAGAACTAGGTCTATACAAAACGTTCGTGATCGCCAAACAGCGTACGCACCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt < 3:1065218/147‑1 (MQ=255) gCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt > 4:946356/1‑62 (MQ=255) ccATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATGTGTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAATAGCGTACGCGCCTGACCCGGCTAGGGGTCGGTGATTACCAGGCTTCCTTCGGtaag > 4:1065218/1‑146 (MQ=255) ccATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCg > 4:196504/1‑87 (MQ=255) ccATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGc > 2:1509791/1‑149 (MQ=255) ttCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt > 1:787471/1‑56 (MQ=255) ttCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCt > 4:476277/1‑149 (MQ=255) tCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTc < 3:1229471/149‑1 (MQ=255) cggcgACGGCGCG‑‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAg > 1:474994/3‑149 (MQ=255) cgAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGGGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCgg < 4:283651/149‑1 (MQ=255) gcgcgc‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTa > 3:1289705/1‑149 (MQ=255) | ATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTC > NC_000913/4285723‑4286018 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |