Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 6.5% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT 6.5% 527.9 / 15.1 168intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (77/78);  new base T (3/8);  total (82/86)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 174 total aligned reads displayed.

AAGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCC  >  NC_000913/1232333‑1232626
                                                                                                                                                    |                                                                                                                                                 
     ggCAGAGGAACACTCTATTTTGCCGGGCAGGGGGTCCAGCAACAATGGTAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATCAGTATAGCGTCAGGAAAGAAGCGCTCCCGCAgg                                                                                                                                              >  2:320599/1‑149 (MQ=255)
      gCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGa                                                                                                                                             <  4:471312/149‑1 (MQ=255)
           ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgct                                                                                                                                        <  1:1566894/149‑2 (MQ=255)
             aaCACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                      <  1:405748/149‑4 (MQ=255)
             aaCACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                      <  3:805373/149‑4 (MQ=255)
             aaCACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                      <  1:1417195/149‑4 (MQ=255)
               cacTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                                                                    <  2:1115543/149‑1 (MQ=255)
               cacTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                                                                    >  2:1752062/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                                                                  <  1:1048440/148‑1 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  2:1141078/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  4:144045/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  4:59166/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  4:804636/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  3:823068/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  1:846401/1‑149 (MQ=255)
                  tctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                 >  2:1434978/1‑149 (MQ=255)
                   ctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAt                                                                                                                                <  1:197586/149‑1 (MQ=255)
                    tATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCAGCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATAGAtt                                                                                                                               >  2:1216633/1‑149 (MQ=255)
                     aTTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                                                                              <  3:676838/149‑1 (MQ=255)
                          gCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                                                                         <  1:263863/149‑1 (MQ=255)
                           ccGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                                                                        >  3:289755/1‑149 (MQ=255)
                              ggCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcgc                                                                                                                                         <  1:628959/129‑1 (MQ=255)
                              ggCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcgc                                                                                                                                         >  2:628959/1‑129 (MQ=255)
                              ggCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGaa                                                                                                                     <  3:1430676/149‑1 (MQ=255)
                                  ggggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                                                                 <  2:1381326/149‑1 (MQ=255)
                                   gggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAt                                                                                                                >  3:862843/1‑149 (MQ=255)
                                     gATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                                                                              <  1:173128/149‑1 (MQ=255)
                                     gATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                                                                              <  1:1082111/149‑1 (MQ=255)
                                      aTCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAc                                                                                                             >  1:132221/1‑149 (MQ=255)
                                             aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtttgtt                                                                                                      >  4:308194/1‑149 (MQ=255)
                                                  tGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                                                                 >  2:110279/1‑149 (MQ=255)
                                                      aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTaaa                                                                                             <  4:224701/149‑1 (MQ=255)
                                                        aTGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACg                                                                                           >  3:1103727/1‑149 (MQ=255)
                                                             gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGCACAGATCACGTTTTTTCGCTTTAAACGGCTgg                                                                                      <  4:1103727/149‑1 (MQ=255)
                                                             gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTgg                                                                                      <  3:810360/149‑1 (MQ=255)
                                                             gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGAGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTgg                                                                                      <  1:358041/149‑1 (MQ=255)
                                                                aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                                                                   >  4:698790/1‑149 (MQ=255)
                                                                  ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                                                                              <  3:451367/88‑1 (MQ=255)
                                                                  ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAAt                                                                                 <  1:89312/149‑1 (MQ=255)
                                                                   cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAACGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGTGAAATc                                                                                >  3:1193467/1‑149 (MQ=255)
                                                                    aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAc                                                                                                                    >  2:215339/1‑112 (MQ=255)
                                                                    aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca                                                                               >  2:580360/1‑149 (MQ=255)
                                                                      gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                                                                             >  1:565144/1‑149 (MQ=255)
                                                                        tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                                                                           >  2:1475108/1‑149 (MQ=255)
                                                                        tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                                                                           >  2:1734839/1‑149 (MQ=255)
                                                                          tCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                                                                         <  1:689671/149‑1 (MQ=255)
                                                                            accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGaa                                                                                                                                     <  1:1121500/87‑1 (MQ=255)
                                                                            accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                                                                       >  3:1003351/1‑149 (MQ=255)
                                                                            accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                                                                       >  1:1640231/1‑149 (MQ=255)
                                                                              cacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                                                                              <  3:34865/108‑1 (MQ=255)
                                                                              cacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAc                                                                     >  4:49486/1‑149 (MQ=255)
                                                                                 gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACa                                                                                                                   <  4:864192/100‑1 (MQ=255)
                                                                                 gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGc                                                                  <  4:915825/149‑1 (MQ=255)
                                                                                    ataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                                                               >  4:1699583/1‑149 (MQ=255)
                                                                                        aaCGGGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGCCAGGAAAAAAGCGCTCCTGCGGGAgcgctt                                                                                                                                       <  1:586939/73‑3 (MQ=38)
                                                                                        aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctt                                                                                                                                       >  2:586939/1‑71 (MQ=39)
                                                                                        aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                           >  4:415105/1‑149 (MQ=255)
                                                                                        aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                           >  3:599600/1‑149 (MQ=255)
                                                                                          cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg                                                         <  3:144045/149‑1 (MQ=255)
                                                                                           gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                        >  2:686218/1‑149 (MQ=255)
                                                                                             gAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                                                                   <  4:511014/120‑1 (MQ=255)
                                                                                             gAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                                                                   >  3:511014/1‑120 (MQ=255)
                                                                                               aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg                                                    <  4:883137/149‑1 (MQ=255)
                                                                                                gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc                                                   <  4:1134638/149‑1 (MQ=255)
                                                                                                gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc                                                   >  3:1355695/1‑149 (MQ=255)
                                                                                                    tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATa                                               >  4:1319341/1‑149 (MQ=255)
                                                                                                     gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaa                                              <  4:289755/149‑1 (MQ=255)
                                                                                                     gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaa                                              >  2:1045257/1‑149 (MQ=255)
                                                                                                      aTTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaa                                             >  3:1703620/1‑149 (MQ=255)
                                                                                                        ttACTGAGAATCAAATACATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGAATTAGCGACCGAACAGATCACGTTTATTAGGTTTAAACGGCTGGGAAATCCCACCCAGCACTGCGACAGTGAGGTAAGGGCTaaaaa                                           <  4:1632911/149‑1 (MQ=255)
                                                                                                           cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                        <  3:1049733/149‑1 (MQ=255)
                                                                                                           cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                        >  1:1450493/1‑149 (MQ=255)
                                                                                                            tGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc                                       >  3:229029/1‑149 (MQ=255)
                                                                                                              aGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGa                                     <  1:1045257/149‑1 (MQ=255)
                                                                                                               gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGag                                    <  3:113104/149‑1 (MQ=255)
                                                                                                                 gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagca                                  <  1:1180120/149‑1 (MQ=255)
                                                                                                                 gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagca                                  <  4:709879/149‑1 (MQ=255)
                                                                                                                   cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagcagc                                <  2:491457/149‑1 (MQ=255)
                                                                                                                   cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagcagc                                <  4:1548478/149‑1 (MQ=255)
                                                                                                                    aaataaaaATAGCGTCAGGAAAAAAACGCTCAAGAAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGCTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                               >  2:1275422/1‑149 (MQ=255)
                                                                                                                     aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                                                                   >  4:1638757/1‑48 (MQ=255)
                                                                                                                     aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                                                                   <  3:1638757/48‑1 (MQ=255)
                                                                                                                     aataaataTAGCGGCAGGAAAAAAGCGCGCCAGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAATGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCa                              >  4:1060026/1‑149 (MQ=255)
                                                                                                                      ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                             >  4:77859/1‑149 (MQ=255)
                                                                                                                      ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                             <  3:698790/149‑1 (MQ=255)
                                                                                                                            ataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGc                       <  4:862843/149‑1 (MQ=255)
                                                                                                                                 ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTc                  >  3:817453/1‑149 (MQ=255)
                                                                                                                                  gCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAACAGATTAGCGAAAGAACAGATCACGTTTTTTTGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATAAGGTAACCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                 <  2:1532603/149‑1 (MQ=255)
                                                                                                                                   cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATGAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGACACTGCGGCATTTCCa                <  2:730497/149‑1 (MQ=255)
                                                                                                                                         ttcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa          >  1:250092/5‑149 (MQ=255)
                                                                                                                                         aaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTaa                                                     >  3:868926/1‑106 (MQ=255)
                                                                                                                                          aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaa         >  1:1638975/1‑149 (MQ=255)
                                                                                                                                          aaaGCGCTCCCGCAGGAGCGCAGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTCTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACAGAGCAGCCACTGCGGCATTTCCAGACCTac         >  4:131010/1‑148 (MQ=255)
                                                                                                                                             gcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaaa      >  2:830214/1‑149 (MQ=255)
                                                                                                                                               gcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAAt    >  1:1494081/1‑149 (MQ=255)
                                                                                                                                                cTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATc   <  2:132221/149‑1 (MQ=255)
                                                                                                                                                 tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATcc  >  3:659340/1‑149 (MQ=255)
                                                                                                                                                   ccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                        <  4:65326/93‑1 (MQ=255)
                                                                                                                                                   ccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                        <  1:127837/109‑1 (MQ=255)
                                                                                                                                                   ccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                        >  2:127837/1‑109 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCC  >  NC_000913/1232333‑1232626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: