Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,588,479 T→A 5.7% N121Y (AAT→TAT)  ydeS ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,588,4790TA 5.7% 603.6 / 20.4 174N121Y (AAT→TAT) ydeSputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (79/85);  new base A (6/4);  total (85/89)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

Only 100 of 174 total aligned reads displayed.

GCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTAATCT  >  NC_000913/1588334‑1588625
                                                                                                                                                 |                                                                                                                                                  
   gAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTc                                                                                                                                              >  2:821170/1‑149 (MQ=255)
   gAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCAAATATGCGTc                                                                                                                                              >  1:1120413/1‑149 (MQ=255)
    aaGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTcc                                                                                                                                             >  1:379798/1‑149 (MQ=255)
    aaGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCAAATATGCGTcc                                                                                                                                             <  4:1034980/149‑1 (MQ=255)
     aGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCAAATATGCGTccg                                                                                                                                            <  2:1001377/149‑2 (MQ=255)
        ccTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                                                                                                                         >  4:668818/1‑149 (MQ=255)
           cACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATAttt                                                                                                                                      <  3:1703101/149‑1 (MQ=255)
           cACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATAttt                                                                                                                                      >  4:2073/1‑149 (MQ=255)
            aCCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTc                                                                                                                                     >  2:1430524/1‑149 (MQ=255)
              cAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCta                                                                                                                                   >  4:1234912/1‑149 (MQ=255)
               aGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCtat                                                                                                                                  <  1:821170/149‑1 (MQ=255)
                gTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCtata                                                                                                                                 <  1:1575416/149‑1 (MQ=255)
                   ccGGATTTACGGACTTCTGAGTTGACTCCATACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACGAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGCCGTAAATTTGCGTCCAATATTTCTATAccc                                                                                                                              >  4:1318167/1‑149 (MQ=255)
                   ccGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATAccc                                                                                                                              >  3:802063/1‑149 (MQ=255)
                   ccGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATAccc                                                                                                                              >  4:923187/1‑149 (MQ=255)
                          gACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCgg                                                                                                                       <  4:1616073/149‑1 (MQ=255)
                           aCGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAATCGGa                                                                                                                      <  4:883884/149‑1 (MQ=255)
                           aCGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGa                                                                                                                      >  2:588010/1‑149 (MQ=255)
                           aCGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGa                                                                                                                      >  2:1100065/1‑149 (MQ=255)
                            cGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATGTTGTTCTGTTCTGGTACCAGTGGGAGCCACTGCCTCCCGGCATGAAGATGATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAg                                                                                                                     <  4:1447502/149‑1 (MQ=255)
                            cGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAg                                                                                                                     <  2:1695023/149‑1 (MQ=255)
                               aCTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGcc                                                                                                                  <  4:1279294/149‑1 (MQ=255)
                               aCTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGcc                                                                                                                  <  4:689788/149‑1 (MQ=255)
                                  tCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTa                                                                                                               <  4:37331/149‑1 (MQ=255)
                                    tGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTAtt                                                                                                             <  3:1276101/149‑1 (MQ=255)
                                      aGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGc                                                                                                           <  1:108898/149‑1 (MQ=255)
                                        ttGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGctt                                                                                                         <  1:991796/149‑1 (MQ=255)
                                          gACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttcc                                                                                                       <  3:1435082/149‑1 (MQ=255)
                                           aCTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttcct                                                                                                      <  1:1027052/149‑1 (MQ=255)
                                           aCTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttcct                                                                                                      <  2:1509286/149‑1 (MQ=255)
                                            cTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttcctt                                                                                                     <  4:390107/149‑1 (MQ=255)
                                             ttCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCgg                                                                                                                       >  3:215025/1‑130 (MQ=255)
                                             ttCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttccttc                                                                                                    >  4:28806/1‑149 (MQ=255)
                                             ttCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttccttc                                                                                                    >  3:485038/1‑149 (MQ=255)
                                             ttCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttccttc                                                                                                    >  3:634152/1‑149 (MQ=255)
                                               cAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATTAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCAt                                                                                                  >  2:1274432/1‑149 (MQ=255)
                                               cAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCAt                                                                                                  <  4:1585278/149‑1 (MQ=255)
                                                    gAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGGCGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGt                                                                                             >  3:827553/1‑149 (MQ=255)
                                                     aGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGtt                                                                                            >  2:336379/1‑149 (MQ=255)
                                                         gAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGcttcct                                                                                                      <  2:521546/135‑1 (MQ=255)
                                                         gAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTcaa                                                                                        <  4:1648011/149‑1 (MQ=255)
                                                            tAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTcaacaa                                                                                     >  4:213892/1‑149 (MQ=255)
                                                              aGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAg                                                                                   >  1:1224545/1‑149 (MQ=255)
                                                               ggCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGt                                                                                  >  4:414431/1‑149 (MQ=255)
                                                                 cAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGtat                                                                                <  2:1321283/149‑1 (MQ=255)
                                                                   aaaTATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGtatta                                                                              >  1:740476/1‑149 (MQ=255)
                                                                     atatTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTattatt                                                                            <  4:562773/149‑1 (MQ=255)
                                                                      tagtgttctgttcTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATGGCTTCCTTCATCCAGTTTCAACAAAGTATTAttt                                                                           >  2:161801/4‑149 (MQ=255)
                                                                        ttGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAGACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTc                                                                         <  1:353093/149‑1 (MQ=255)
                                                                         tgttctgttcTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCt                                                                        <  3:696025/149‑1 (MQ=255)
                                                                         tgttctgttcTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCt                                                                        <  2:1198046/149‑1 (MQ=255)
                                                                           ttctgttctgGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCAGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGc                                                                      >  2:1059385/1‑149 (MQ=255)
                                                                               gttctgGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATcc                                                                  <  4:1699020/149‑1 (MQ=255)
                                                                                   tgGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCaa                                                              >  1:1471121/1‑149 (MQ=255)
                                                                                   tgGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCaa                                                              >  3:231921/1‑149 (MQ=255)
                                                                                   tgGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTACACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCaa                                                              <  1:819940/149‑1 (MQ=255)
                                                                                      tACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACaaa                                                                                    <  2:1466560/124‑1 (MQ=255)
                                                                                      tACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACaaa                                                                                    >  1:1466560/1‑124 (MQ=255)
                                                                                        ccAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCg                                                         >  1:283973/1‑149 (MQ=255)
                                                                                           gTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTa                                                      <  3:414431/149‑1 (MQ=255)
                                                                                            tGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTaa                                                     >  1:355722/1‑149 (MQ=255)
                                                                                             gggATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTaaa                                                    <  4:1334761/149‑1 (MQ=255)
                                                                                              ggATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAAt                                                   <  2:117803/149‑1 (MQ=255)
                                                                                              ggATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATAATTCTATAACCAAACCAGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATCACGTGAAAt                                                   >  1:1366950/1‑149 (MQ=255)
                                                                                                 tCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGTGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCa                                                >  3:867237/1‑149 (MQ=255)
                                                                                                 tCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCa                                                >  1:340344/1‑149 (MQ=255)
                                                                                                  ccACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCaa                                               <  3:1336868/149‑1 (MQ=255)
                                                                                                   cACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAAc                                              <  4:757153/149‑1 (MQ=255)
                                                                                                    aCTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAAcc                                             >  3:595101/1‑149 (MQ=255)
                                                                                                    aCTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAAcc                                             >  3:879149/1‑149 (MQ=255)
                                                                                                      tGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCg                                           >  2:590266/1‑149 (MQ=255)
                                                                                                            ccGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAAcccc                                     <  3:500484/149‑1 (MQ=255)
                                                                                                            ccGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAAcccc                                     >  4:804106/1‑149 (MQ=255)
                                                                                                            ccGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAAcccc                                     >  1:216026/1‑149 (MQ=255)
                                                                                                             cGGCATGAAGAACATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAAccccc                                    >  4:1217576/1‑149 (MQ=255)
                                                                                                                  tGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGt                               <  3:19737/149‑1 (MQ=255)
                                                                                                                      gATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTcaa                                                                                        <  1:1759125/88‑1 (MQ=255)
                                                                                                                      gATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCt                                                                        >  2:905845/1‑104 (MQ=255)
                                                                                                                      gATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCt                                                                        <  1:905845/104‑1 (MQ=255)
                                                                                                                         cATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTtg                        <  1:1100065/149‑1 (MQ=255)
                                                                                                                         cATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTtg                        <  4:826739/149‑1 (MQ=255)
                                                                                                                          aTTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTg                                >  3:360419/1‑140 (MQ=255)
                                                                                                                           ttCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTtgct                      >  2:296907/1‑149 (MQ=255)
                                                                                                                               gTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCAt                  <  4:231921/149‑1 (MQ=255)
                                                                                                                                     ttggACGCAAATATGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACGCGAACCCCCGTTGTCCCTTTGCTGCATTCACtt            >  4:1140714/3‑149 (MQ=255)
                                                                                                                                     ttggACGCAAATATGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACtt            >  2:365724/3‑149 (MQ=255)
                                                                                                                                     ttggACGCAAATATGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACtt            >  1:1239597/3‑149 (MQ=255)
                                                                                                                                     ccGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACtt            <  3:392283/149‑1 (MQ=255)
                                                                                                                                       ggACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTaa          <  4:1358664/149‑1 (MQ=255)
                                                                                                                                       ggACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTaa          <  1:1689943/149‑1 (MQ=255)
                                                                                                                                       ggACGCAAATATGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTaa          >  1:491841/1‑149 (MQ=255)
                                                                                                                                        ggcGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAAc         <  4:827553/147‑1 (MQ=255)
                                                                                                                                        gACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAAc         <  1:1047846/149‑1 (MQ=255)
                                                                                                                                           gCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTa      >  2:1671363/1‑149 (MQ=255)
                                                                                                                                            cATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTaa          <  1:1332469/144‑1 (MQ=255)
                                                                                                                                            cATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTaa     <  2:740476/149‑1 (MQ=255)
                                                                                                                                            cATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTaa     <  3:1554632/149‑1 (MQ=255)
                                                                                                                                             atatTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCa               <  2:33667/138‑1 (MQ=255)
                                                                                                                                             atatTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTAAt    >  2:216479/1‑149 (MQ=255)
                                                                                                                                               atTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTAATCt  >  1:1568398/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCATCCAGTTTCAACAAAGTATTATTTTCTGCATCCTCAATACCGTTAAATGCAACCCGAACCCCCGTTGTCCCTTTGCTGCATTCACTTAACGTAATCT  >  NC_000913/1588334‑1588625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: