Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,603,724 A→C 5.2% T236P (ACC→CCC)  lsrC → autoinducer 2 import system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,7240AC 5.2% 575.3 / 21.7 173T236P (ACC→CCC) lsrCautoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base A (82/82);  new base C (3/6);  total (85/88)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

Only 100 of 173 total aligned reads displayed.

TATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCA  >  NC_000913/1603577‑1603868
                                                                                                                                                   |                                                                                                                                                
tATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGAc                                                                                                                                                 >  1:1047288/1‑149 (MQ=255)
 aTGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGAcc                                                                                                                                                <  2:1226869/149‑1 (MQ=255)
   gCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCgg                                                                                                                                              <  2:1622651/149‑1 (MQ=255)
   gCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCgg                                                                                                                                              >  1:183516/1‑149 (MQ=255)
    ccACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGt                                                                                                                                             >  4:1056461/1‑149 (MQ=255)
     aaCGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCCTTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCTACCAGACCGGTa                                                                                                                                            <  4:1457601/148‑1 (MQ=255)
         ggCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCgg                                                                                                                                        <  3:915162/149‑1 (MQ=255)
           cGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGc                                                                                                                                      <  3:531236/149‑1 (MQ=255)
            gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                     >  4:713362/1‑149 (MQ=255)
            gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                     >  4:182740/1‑149 (MQ=255)
              tAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTgg                                                                                                                                   <  4:1595614/149‑1 (MQ=255)
              tAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTgg                                                                                                                                   <  2:901932/149‑1 (MQ=255)
               aaTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                  <  2:329227/149‑1 (MQ=255)
                aTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggtt                                                                                                                                 <  1:1069697/149‑3 (MQ=255)
                aTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGgag                                                                                                                                 <  4:540146/149‑1 (MQ=255)
                 tttACAGGGCGCTCGTCAACTGGGTGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAGCCAGACCGGTACCGGGCTGgaga                                                                                                                                <  1:354890/149‑1 (MQ=255)
                  ttACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGAt                                                                                                                               >  4:1524495/1‑149 (MQ=255)
                  ttACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGAt                                                                                                                               >  1:1351691/1‑149 (MQ=255)
                    aCAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGa                                                                                                                             >  4:754235/1‑149 (MQ=255)
                    aCAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGa                                                                                                                             <  4:924061/149‑1 (MQ=255)
                      aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                           <  4:468509/149‑1 (MQ=255)
                      aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                           <  4:649791/149‑1 (MQ=255)
                        ggCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGc                                                                                                                         <  1:360410/149‑1 (MQ=255)
                          cgcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCaa                                                                                                                       <  1:1460049/149‑1 (MQ=255)
                          cgcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTGTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCaa                                                                                                                       <  3:1056461/149‑1 (MQ=255)
                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAGCCAGACCGGTACCGGGCTGGAGATGAAAGCAAt                                                                                                                      <  1:837308/149‑1 (MQ=255)
                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                  >  3:156977/1‑137 (MQ=255)
                           gcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                  <  4:156977/137‑1 (MQ=255)
                             tCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTg                                                                                                                    >  1:420621/1‑149 (MQ=255)
                               gTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCa                                                                                                                  <  1:532636/149‑1 (MQ=255)
                               gTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCAATTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCa                                                                                                                  >  3:1127500/1‑149 (MQ=255)
                                tCAACTGTGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAACAATTGCa                                                                                                                  >  2:937416/1‑148 (MQ=255)
                                tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTgg                                                                                                                                   >  2:34992/1‑131 (MQ=255)
                                    cTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTg                                                                                                             >  1:174312/1‑149 (MQ=255)
                                     tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCggg                                                                                                                                       <  3:104579/122‑1 (MQ=255)
                                     tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGGTGAAAGCAATTGCAGCCTGc                                                                                                            >  4:1323697/1‑149 (MQ=255)
                                          gTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCt                                                                                                       >  1:984794/1‑149 (MQ=255)
                                                  tGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGggcg                                                                                                  >  1:1260408/1‑146 (MQ=255)
                                                   gAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCAt                                                                                              <  2:461830/149‑1 (MQ=255)
                                                       ccATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCa                                                                                                                  >  3:1576712/1‑125 (MQ=255)
                                                         aTTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                        >  3:441174/1‑149 (MQ=255)
                                                          ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTg                                                                                       <  1:163931/149‑1 (MQ=255)
                                                          ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTg                                                                                       <  2:1178122/149‑1 (MQ=255)
                                                           tCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCCGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGc                                                                                      >  4:376978/1‑149 (MQ=255)
                                                            cGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCt                                                                                     <  2:1047288/149‑1 (MQ=255)
                                                               aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGt                                                                                                          <  2:337769/125‑1 (MQ=255)
                                                               aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGt                                                                                                          >  1:337769/1‑125 (MQ=255)
                                                                ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCggt                                                                                 <  1:1393926/149‑1 (MQ=255)
                                                                   tgGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAg                                                                                                                 <  4:1009288/114‑1 (MQ=255)
                                                                   tgGCATTTTCGCTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAg                                                                                                                 >  3:1009288/1‑114 (MQ=255)
                                                                       aTTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCg                                                                          >  1:780853/1‑149 (MQ=255)
                                                                         tttCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                        >  3:730100/1‑149 (MQ=255)
                                                                             gTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggttgg                                                                                                                               >  3:612560/1‑86 (MQ=39)
                                                                             gTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGa                                                                    >  2:1666817/1‑149 (MQ=255)
                                                                              ttGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                        <  4:1225583/144‑1 (MQ=255)
                                                                                  aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGattatt                                                               >  4:1720289/1‑149 (MQ=255)
                                                                                  aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCAtt                                                               <  3:182740/149‑1 (MQ=255)
                                                                                  aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCAtt                                                               <  4:409950/149‑1 (MQ=255)
                                                                                   cGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTg                                                              <  2:621515/149‑1 (MQ=255)
                                                                                   cGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTg                                                              <  3:1473215/149‑1 (MQ=255)
                                                                                     gCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGt                                                            <  3:1202139/149‑1 (MQ=255)
                                                                                     gCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCCTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCCGGGCGGCATTAGTTTGCGCGGTGGTTCCGGTGCGATCATTGGt                                                            <  4:1065934/149‑1 (MQ=255)
                                                                                       tGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGc                                                          <  3:764595/149‑1 (MQ=255)
                                                                                       tGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGc                                                          <  3:206947/149‑1 (MQ=255)
                                                                                                 cACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGATCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGgc                                                >  2:1501351/1‑149 (MQ=255)
                                                                                                     ggCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCAt                                            <  1:875953/149‑1 (MQ=255)
                                                                                                     ggCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCAt                                            <  2:410728/149‑1 (MQ=255)
                                                                                                      gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCCGCCTGCGTGCTCGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATg                                           >  2:1251108/1‑149 (MQ=255)
                                                                                                         ggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGtt                                                                                         >  1:185735/1‑100 (MQ=255)
                                                                                                         ggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGtt                                        >  3:741031/1‑149 (MQ=255)
                                                                                                         ggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGtt                                        <  2:769917/149‑1 (MQ=255)
                                                                                                           aaTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTcc                                      <  4:605869/149‑1 (MQ=255)
                                                                                                            aTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCACCCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCt                                     <  4:741031/149‑1 (MQ=255)
                                                                                                              tgtgTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                   <  3:982761/149‑1 (MQ=255)
                                                                                                                tgtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACg                                 >  3:911601/1‑149 (MQ=255)
                                                                                                                  tttGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCa                               >  3:1518631/1‑149 (MQ=255)
                                                                                                                    tGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGa                             <  3:1323697/149‑1 (MQ=255)
                                                                                                                      cTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGt                                                            <  2:1347721/116‑1 (MQ=255)
                                                                                                                      cTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATc                           >  1:1661160/1‑149 (MQ=255)
                                                                                                                      cTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATc                           <  4:198828/149‑1 (MQ=255)
                                                                                                                       ttCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                   <  3:295333/140‑1 (MQ=255)
                                                                                                                       ttCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                   >  4:295333/1‑140 (MQ=255)
                                                                                                                          gCAGATTGGTTTTCTCCCCGACCAGACCGGTAGCGGGCTGGAGATGAAAGCGATTGCAGCCTGCGTGCTGGGCGGCATTAATTTGCTCGGTGGTACCGGGGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                       <  2:12025/149‑1 (MQ=255)
                                                                                                                          gCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                       <  1:749876/149‑1 (MQ=255)
                                                                                                                             gATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCg                    >  3:1145139/1‑149 (MQ=255)
                                                                                                                              aTTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGc                                >  2:976509/1‑136 (MQ=255)
                                                                                                                              aTTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGc                                <  1:976509/136‑1 (MQ=255)
                                                                                                                                   ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGtt                                                                                         >  2:85300/1‑74 (MQ=255)
                                                                                                                                        acaacAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTg         >  4:239714/5‑149 (MQ=255)
                                                                                                                                          cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt       >  1:1193834/1‑149 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCAt                                                                >  4:140143/1‑91 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt       >  1:1346085/1‑148 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt       <  2:1346085/148‑1 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg      >  3:424079/1‑149 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg      >  3:959859/1‑149 (MQ=255)
                                                                                                                                           ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg      >  2:218912/1‑149 (MQ=255)
                                                                                                                                            cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc     <  2:183516/149‑1 (MQ=255)
                                                                                                                                            cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc     <  4:968670/149‑1 (MQ=255)
                                                                                                                                               ccAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCa  >  4:1171797/1‑149 (MQ=255)
                                                                                                                                                  gACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc     <  2:522133/143‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCA  >  NC_000913/1603577‑1603868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: