Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,954,256 T→A 6.4% I272N (ATT→AAT)  cmoB → tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,954,2560TA 6.4% 693.2 / 27.7 204I272N (ATT→AAT) cmoBtRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (93/98);  new base A (8/5);  total (101/103)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 206 total aligned reads displayed.

CTGGAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGC  >  NC_000913/1954111‑1954402
                                                                                                                                                 |                                                                                                                                                  
cTGGAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGc                                                                                                                                                 <  1:107139/149‑1 (MQ=255)
   gAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGa                                                                                                                                              >  1:1036782/1‑149 (MQ=255)
    aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                                                                                                             <  1:1127879/149‑1 (MQ=255)
    aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                                                                                                             <  3:621431/149‑1 (MQ=255)
    aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                                                                                                             <  1:171604/149‑1 (MQ=255)
      aCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAtgt                                                                                                                                           <  3:864801/149‑1 (MQ=255)
      aCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAtgt                                                                                                                                           <  4:688153/149‑1 (MQ=255)
       cGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCAATGCGGAtatc                                                                                                                                          <  3:842328/149‑4 (MQ=255)
       cGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCAATGCGGAtatc                                                                                                                                          <  2:730942/149‑4 (MQ=255)
        gCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa                                                                                                                                         <  4:1445071/149‑1 (MQ=255)
        gCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa                                                                                                                                         >  4:1086575/1‑149 (MQ=255)
        gCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa                                                                                                                                         <  2:1706895/149‑1 (MQ=255)
          tGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGc                                                                                                                                       <  1:1002131/149‑1 (MQ=255)
          tGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGc                                                                                                                                       <  2:1287956/149‑1 (MQ=255)
            gTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGt                                                                                                                                     <  4:1166061/149‑1 (MQ=255)
                ttGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGCAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTacc                                                                                                                                 >  4:1137377/1‑149 (MQ=255)
                         atgaGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaa                                                                                                                        <  1:163589/149‑1 (MQ=255)
                         atgaGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAGGTGAGCGTTACCACCACagaa                                                                                                                        <  1:1258749/149‑1 (MQ=255)
                            agaACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAACAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAagag                                                                                                                     >  3:335152/1‑149 (MQ=255)
                             gaAGACGGTGCTTGTACCCAGCGGTCGTTACGCGCACATGCGCAATGGCTAGTTCATCCCTTCCGCGCTGTCCCTGAAAAACTGGCTGAAGAAGTGTTGATTGGTAGATATCCGCATTGCGGCTGTGAGCGTTACCACCACAGAACagc                                                                                                                    >  4:35204/1‑149 (MQ=255)
                             gaACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagc                                                                                                                    >  4:856769/1‑149 (MQ=255)
                              aaCACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagca                                                                                                                   >  2:547459/1‑149 (MQ=255)
                               acacGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagcag                                                                                                                  <  3:6669/149‑1 (MQ=255)
                                   ggTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGAc                                                                                                              >  3:503870/1‑149 (MQ=255)
                                            aCCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAat                                                                                                     <  4:265050/149‑1 (MQ=255)
                                             ccAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg                                                                                                    <  2:142659/149‑1 (MQ=255)
                                               aGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatgga                                                                                                  >  1:1469436/1‑149 (MQ=255)
                                               aGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatgga                                                                                                  >  1:160426/1‑149 (MQ=255)
                                               aGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatgga                                                                                                  >  4:1324053/1‑149 (MQ=255)
                                                ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTacc                                                                                                                                 >  2:314622/1‑117 (MQ=255)
                                                ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatggat                                                                                                 >  4:43409/1‑149 (MQ=255)
                                                 gCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatggatg                                                                                                <  2:1139381/149‑1 (MQ=255)
                                                     tCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagc                                                                                                                    >  2:840699/1‑125 (MQ=255)
                                                     tCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagc                                                                                                                    <  1:840699/125‑1 (MQ=255)
                                                       gTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTAcc                                                                                          >  3:588760/1‑149 (MQ=255)
                                                       gTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTAcc                                                                                          <  4:1253798/149‑1 (MQ=255)
                                                            gcgcAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATc                                                                                     >  4:836751/1‑149 (MQ=255)
                                                                  aTGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGc                                                                               >  2:1325958/1‑149 (MQ=255)
                                                                       tAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTg                                                                                 >  3:1277057/1‑142 (MQ=255)
                                                                       tAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGAtt                                                                          >  2:1081529/1‑149 (MQ=255)
                                                                       tAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGAtt                                                                          >  2:758149/1‑149 (MQ=255)
                                                                        aaTGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGAttt                                                                         >  4:1523696/1‑149 (MQ=255)
                                                                         aTGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTc                                                                        >  4:1424091/1‑149 (MQ=255)
                                                                            tCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTc                                                                     <  4:595212/149‑1 (MQ=255)
                                                                            tCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTc                                                                     >  3:891686/1‑149 (MQ=255)
                                                                             cTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg                                                                                                    <  1:331384/117‑1 (MQ=255)
                                                                              tATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTcc                                                                       >  2:160682/1‑145 (MQ=255)
                                                                              tATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa                                                                   >  1:668811/1‑149 (MQ=255)
                                                                               aTTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGCGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAc                                                                  <  3:173788/149‑1 (MQ=255)
                                                                                tttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAcc                                                                 >  2:1127848/1‑149 (MQ=255)
                                                                                 ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAccc                                                                <  3:1324053/149‑1 (MQ=255)
                                                                                  tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCca                                                               >  1:789114/1‑149 (MQ=255)
                                                                                    aTCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg                                                                                                    <  3:950419/110‑1 (MQ=255)
                                                                                    aTCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCcaca                                                             >  1:219963/1‑149 (MQ=255)
                                                                                     tCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCAcc                                                                                                         <  4:1348257/104‑1 (MQ=255)
                                                                                     tCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACAt                                                            <  1:180682/149‑1 (MQ=255)
                                                                                      cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGGAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaa                                                                                                                        <  3:789147/88‑1 (MQ=255)
                                                                                      cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATg                                                           >  4:204096/1‑149 (MQ=255)
                                                                                       ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTcc                                                                       <  3:1052/136‑1 (MQ=255)
                                                                                        cTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg                                                                                                    >  4:1675582/1‑106 (MQ=255)
                                                                                        cTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGAt                                                         >  1:897449/1‑149 (MQ=255)
                                                                                         ttCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATc                                                        >  3:1584153/1‑149 (MQ=255)
                                                                                            cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg                                                     <  3:204096/149‑1 (MQ=255)
                                                                                            cgcgCTGGCGCTGAAAAACTGGCAGAAGAAGTATGGTTTTGTAGATATCAGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg                                                     <  1:637889/149‑1 (MQ=255)
                                                                                             gcgctggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCggg                                                    <  2:943607/149‑1 (MQ=255)
                                                                                             gcgctggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCggg                                                    <  2:91218/149‑1 (MQ=255)
                                                                                             gcgctggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg                                                     <  2:1035809/148‑1 (MQ=255)
                                                                                                      ctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt                                           <  4:1158579/149‑1 (MQ=255)
                                                                                                       tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTg                                          >  3:1112461/1‑149 (MQ=255)
                                                                                                       tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCAGCATTTTGAATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTACTCGACCCACATGATCCGGGTAAAACGGTg                                          >  4:929744/1‑149 (MQ=255)
                                                                                                          aaaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGaa                                       <  3:43409/149‑1 (MQ=255)
                                                                                                          aaaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATAGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTACAACGGTGGaa                                       <  4:1498442/149‑1 (MQ=255)
                                                                                                            aaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt                                           >  3:399021/1‑143 (MQ=255)
                                                                                                            aaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt                                           <  4:399021/143‑1 (MQ=255)
                                                                                                            aaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAgg                                     <  1:1150495/149‑1 (MQ=255)
                                                                                                            aaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAgg                                     <  2:335432/149‑1 (MQ=255)
                                                                                                                 gCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg                                                     >  3:488189/1‑128 (MQ=255)
                                                                                                                 gCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg                                                     <  4:488189/128‑1 (MQ=255)
                                                                                                                  cTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATcc                               <  4:771706/149‑1 (MQ=255)
                                                                                                                   tGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCg                              >  2:1151594/1‑149 (MQ=255)
                                                                                                                    gaagaaGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCgg                             >  4:1639687/1‑149 (MQ=255)
                                                                                                                    gaagaaGTGTGGTTTTGTAGATATACGCATTGCGGATGTTAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGCAGGTTATCCgg                             <  3:1137377/149‑1 (MQ=255)
                                                                                                                               gTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGc                                                                                   <  2:793089/84‑1 (MQ=255)
                                                                                                                               gTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGc                                                                                   >  1:793089/1‑84 (MQ=255)
                                                                                                                               gTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgc                  >  1:991372/1‑149 (MQ=255)
                                                                                                                                 tttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc                <  2:160426/149‑1 (MQ=255)
                                                                                                                                    gTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTc                                                                        >  1:1400812/1‑90 (MQ=255)
                                                                                                                                     tAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCgg              >  3:1354013/1‑147 (MQ=255)
                                                                                                                                     ctcacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTg            >  4:79279/6‑149 (MQ=255)
                                                                                                                                      tcacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc           >  3:769226/5‑149 (MQ=255)
                                                                                                                                      tcacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc           >  2:1391070/5‑149 (MQ=255)
                                                                                                                                      acacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGTGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc           >  2:1490952/5‑149 (MQ=255)
                                                                                                                                      aGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc           >  2:1207893/1‑149 (MQ=255)
                                                                                                                                       gATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCt          <  4:1713956/149‑1 (MQ=255)
                                                                                                                                         tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGa        <  1:313690/149‑1 (MQ=255)
                                                                                                                                         catCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGa        >  2:484189/2‑149 (MQ=255)
                                                                                                                                          atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAt       >  1:1569618/1‑149 (MQ=255)
                                                                                                                                           tCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAtt      >  1:36481/1‑149 (MQ=255)
                                                                                                                                           tCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAtt      >  2:3579/1‑149 (MQ=255)
                                                                                                                                               cATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgc  <  3:1453603/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGGAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGC  >  NC_000913/1954111‑1954402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: