Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,858 G→C 5.7% intergenic (‑100/+15) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,8580GC 5.7% 739.7 / 28.1 210intergenic (‑100/+15)yecJ/azuCDUF2766 family protein/acid‑inducible small membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (104/94);  new base C (3/9);  total (107/103)
Fisher's exact test for biased strand distribution p-value = 7.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 214 total aligned reads displayed.

CAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTG  >  NC_000913/1987710‑1988002
                                                                                                                                                     |                                                                                                                                                 
caaCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                   <  4:1337891/149‑1 (MQ=255)
 aaCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCgg                                                                                                                                                  >  4:1167575/1‑149 (MQ=255)
       aTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAgg                                                                                                                                            <  1:1671724/149‑1 (MQ=255)
          aGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttt                                                                                                                                        <  1:1055454/149‑1 (MQ=255)
          aGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttt                                                                                                                                        >  4:1053161/1‑149 (MQ=255)
           gAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGtttt                                                                                                                                       <  3:419995/149‑1 (MQ=255)
            aaaCTAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttttt                                                                                                                                      >  2:360463/1‑149 (MQ=255)
            aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttttt                                                                                                                                      <  2:1542004/149‑1 (MQ=255)
            aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttttt                                                                                                                                      <  4:1292482/149‑1 (MQ=255)
             aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                     <  4:1559122/149‑1 (MQ=255)
             aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAACCTGCTCCGGCAGGttttttt                                                                                                                                    <  4:1610349/149‑2 (MQ=255)
             aaCCAATTCCTGATCCGCATTCCGCAGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCCTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                     <  2:1141893/149‑1 (MQ=255)
              aCCAATTCGTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTg                                                                                                                                    >  3:1212885/1‑149 (MQ=255)
               ccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                   >  1:1580098/1‑149 (MQ=255)
               ccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                   >  2:883404/1‑149 (MQ=255)
               ccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                   >  1:1199285/1‑149 (MQ=255)
                 aaTTCCTGATCAGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGACGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTa                                                                                                                                 >  1:1432108/1‑149 (MQ=255)
                   ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                               <  1:691185/149‑1 (MQ=255)
                   ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                               >  1:820232/1‑149 (MQ=255)
                   ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                               <  4:1219990/149‑1 (MQ=255)
                      cTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttt                                                                                                                                        <  3:1165127/137‑1 (MQ=255)
                       tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCGGGTTTTTTGTTATCGGaa                                                                                                                           <  2:1027767/149‑1 (MQ=255)
                       tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGaa                                                                                                                           <  2:985654/149‑1 (MQ=255)
                           cGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACata                                                                                                                       <  4:167048/149‑1 (MQ=255)
                                ttCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGcc                                                                                                                  >  1:648024/1‑149 (MQ=255)
                                 tCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCt                                                                                                                 <  3:1479873/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                         <  3:1499475/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                         <  2:1648758/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                         <  4:836156/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                         <  2:1391693/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                         <  2:147872/149‑1 (MQ=255)
                                          gggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACg                                                                                                        >  2:408716/1‑149 (MQ=255)
                                          gggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACg                                                                                                        >  1:1743235/1‑149 (MQ=255)
                                           ggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGt                                                                                                       >  4:907242/1‑149 (MQ=255)
                                           ggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTAACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAATACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTActt                                                                                                       <  2:1199285/149‑3 (MQ=255)
                                               aTCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt                                                                                                   <  4:1409277/149‑1 (MQ=255)
                                               aTCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttg                                                                                                   >  1:1551551/1‑148 (MQ=255)
                                                tCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTACAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTg                                                                                                  <  2:425333/149‑1 (MQ=255)
                                                 cGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGa                                                                                                 <  4:1683003/149‑1 (MQ=255)
                                                  gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGtttttt                                                                                                                                     >  4:346904/1‑112 (MQ=255)
                                                  gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                     <  1:1345719/112‑1 (MQ=255)
                                                  gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                               >  2:1062105/1‑118 (MQ=255)
                                                   tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                               <  3:1123838/149‑1 (MQ=255)
                                                        tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCtt                                                                                          >  1:1169515/1‑149 (MQ=255)
                                                        tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCtt                                                                                          >  3:923106/1‑149 (MQ=255)
                                                             aTAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAAt                                                                                     >  1:1112768/1‑149 (MQ=255)
                                                                gCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGt                                                                                  <  1:940335/149‑1 (MQ=255)
                                                                   ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                               >  2:123922/1‑149 (MQ=255)
                                                                         aaGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                                         >  2:1455795/1‑149 (MQ=255)
                                                                          aGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACAt                                                                        >  2:330956/1‑149 (MQ=255)
                                                                            ccGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATAc                                                                      >  4:681114/1‑149 (MQ=255)
                                                                             cGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACt                                                                     >  1:26082/1‑149 (MQ=255)
                                                                              gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtt                                                                    >  1:1012554/1‑149 (MQ=255)
                                                                                  tagtagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa                                                                <  3:1053161/149‑1 (MQ=255)
                                                                                    gtagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAgg                                                              >  3:960682/1‑149 (MQ=255)
                                                                                      agCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAtt                                                                              <  1:596186/131‑1 (MQ=255)
                                                                                       gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtt                                                           >  3:94455/1‑149 (MQ=255)
                                                                                       gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtt                                                           >  2:1314080/1‑149 (MQ=255)
                                                                                         gACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                         >  2:595865/1‑149 (MQ=255)
                                                                                         gACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGATATTGAACATACTTTTCAGGAtttt                                                         <  4:144374/149‑1 (MQ=255)
                                                                                               acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg                                                                                         <  2:67904/111‑1 (MQ=255)
                                                                                                caTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGt                                                  >  4:142981/1‑149 (MQ=255)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                 <  4:825220/149‑1 (MQ=255)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                 >  4:558401/1‑149 (MQ=255)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                 >  3:6200/1‑149 (MQ=255)
                                                                                                   cTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                                            <  3:214490/104‑1 (MQ=255)
                                                                                                   cTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                                            >  4:214490/1‑104 (MQ=255)
                                                                                                   cTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTc                                               <  1:1517529/149‑1 (MQ=255)
                                                                                                      cAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATc                                            >  3:1443249/1‑149 (MQ=255)
                                                                                                           aTAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGc                                       >  1:67734/1‑149 (MQ=255)
                                                                                                                 acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCg                                 >  3:1262360/1‑149 (MQ=255)
                                                                                                                   aTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                               >  4:405732/1‑149 (MQ=255)
                                                                                                                    tCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc                              >  2:600283/1‑149 (MQ=255)
                                                                                                                       aCCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAtt                           <  3:1379293/149‑1 (MQ=255)
                                                                                                                        ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                          <  3:681114/149‑1 (MQ=255)
                                                                                                                         cGTCATCAAGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTg                         <  2:1432108/149‑1 (MQ=255)
                                                                                                                          gTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGt                        <  3:1339592/149‑1 (MQ=255)
                                                                                                                           tcatcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                       <  3:907242/149‑1 (MQ=255)
                                                                                                                           tcatcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATACTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                       <  4:1057134/149‑1 (MQ=255)
                                                                                                                              tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata                    >  3:815358/1‑149 (MQ=255)
                                                                                                                                  gACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAgg                >  1:1325226/1‑149 (MQ=255)
                                                                                                                                   acacAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTTAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt               <  2:820232/149‑1 (MQ=255)
                                                                                                                                   acacAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt               <  3:1359914/149‑1 (MQ=255)
                                                                                                                                     aacaaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGAAGCACTCCGACCATTTGTTATACAGgtgt             >  3:487436/4‑149 (MQ=255)
                                                                                                                                      caAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGtt            <  2:1112768/149‑1 (MQ=255)
                                                                                                                                       aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa           >  4:946815/1‑149 (MQ=255)
                                                                                                                                       aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa           <  1:960254/149‑1 (MQ=255)
                                                                                                                                       aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa           >  3:269219/1‑149 (MQ=255)
                                                                                                                                        aaaaaaCCTGCTCCGGCAGGTTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa           >  3:1553599/1‑149 (MQ=255)
                                                                                                                                        aaaaaaCCTGCTCCGGCAGGTTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                       <  3:76910/137‑1 (MQ=255)
                                                                                                                                        aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt          <  3:558401/149‑1 (MQ=255)
                                                                                                                                         aaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTg                                                                             >  2:490957/1‑81 (MQ=255)
                                                                                                                                         aaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTg                                                                             <  1:490957/81‑1 (MQ=255)
                                                                                                                                         aaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt         <  2:648024/149‑1 (MQ=255)
                                                                                                                                         aaaaaCCTGCCGGAGCAGCTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTTATCGCGGCACTCCGACCATTTGt                        >  4:134853/1‑134 (MQ=255)
                                                                                                                                          aaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTg        >  1:1134470/1‑149 (MQ=255)
                                                                                                                                               cTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCttt   <  3:144984/149‑1 (MQ=255)
                                                                                                                                               cTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTAATTGTCttt   <  4:1485877/149‑1 (MQ=255)
                                                                                                                                                tGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATATTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTg  >  2:289606/1‑149 (MQ=255)
                                                                                                                                                tGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTg  <  1:422369/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTG  >  NC_000913/1987710‑1988002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: