BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,991,866	T→A	6.3%	noncoding (36/87 nt)	leuZ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,991,866	0	T	A	6.3%	659.2 / 24.4	191	noncoding (36/87 nt)	leuZ	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base T (79/100); new base A (8/4); total (87/104)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 195 total aligned reads displayed.

AAGGAGGGTGTAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAG  >  NC_000913/1991718‑1992011

     gggTGTAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATc                                                                                                                                              <  1:1281663/149‑1 (MQ=255)

        tgtAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCt                                                                                                                                           <  4:522484/149‑1 (MQ=255)

        tgtAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCt                                                                                                                                           <  2:689812/149‑1 (MQ=255)

          tAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtg                                                                                                                                         >  2:637438/1‑149 (MQ=255)

          tAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtcg                                                                                                                                         <  4:225237/149‑3 (MQ=255)

           aaCGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtgt                                                                                                                                        <  3:523066/149‑1 (MQ=255)

                cAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTa                                                                                                                                   <  2:1223847/149‑1 (MQ=255)

                 aaGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTAc                                                                                                                                  <  2:385411/149‑1 (MQ=255)

                 aaGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACAATGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTAc                                                                                                                                  <  4:1136652/149‑1 (MQ=255)

                  aGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTAcc                                                                                                                                 <  3:1213041/149‑1 (MQ=255)

                    ttGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGa                                                                                                                               >  1:666950/1‑149 (MQ=255)

                      gCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGAtt                                                                                                                             >  2:1155487/1‑149 (MQ=255)

                        aGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                           >  1:1362114/1‑149 (MQ=255)

                        aGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                           <  2:399010/149‑1 (MQ=255)

                            acaAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtgt                                                                                                                                        <  4:786912/132‑1 (MQ=255)

                            acaAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccacca                                                                                                                       >  3:345781/1‑149 (MQ=255)

                              aaagaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATc                                                                                                                     >  1:1103287/1‑149 (MQ=255)

                               ataaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATcc                                                                                                                    >  2:1396247/3‑149 (MQ=255)

                               aaaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATcc                                                                                                                    >  2:928497/1‑149 (MQ=255)

                                aaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCg                                                                                                                   >  4:1384764/1‑149 (MQ=255)

                                aaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCg                                                                                                                   <  2:1340997/149‑1 (MQ=255)

                                   aaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGc                                                                                                                >  1:365105/1‑149 (MQ=255)

                                      caccCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCg                                                                                                             >  1:1043851/1‑149 (MQ=255)

                                       accCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCgg                                                                                                            >  4:734518/1‑149 (MQ=255)

                                        cccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtgt                                                                                                                                        >  3:795683/1‑120 (MQ=255)

                                        cccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCggg                                                                                                           <  3:1493910/149‑1 (MQ=255)

                                         ccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGga                                                                                                          >  1:1043602/1‑149 (MQ=255)

                                          cGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaa                                                                                                         <  3:1384764/149‑1 (MQ=255)

                                          cGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaa                                                                                                         <  3:221684/149‑1 (MQ=255)

                                          cGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaa                                                                                                         >  2:1455569/1‑149 (MQ=255)

                                                tggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt                                                                                                   <  1:488428/149‑1 (MQ=255)

                                                  gtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg                                                                                                 >  3:1682387/1‑149 (MQ=255)

                                                   tttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa                                                                                                >  3:1164601/1‑149 (MQ=255)

                                                   tttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa                                                                                                <  1:698902/149‑1 (MQ=255)

                                                    ttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAg                                                                                               <  4:1357708/149‑1 (MQ=255)

                                                     tCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAgg                                                                                              <  3:621043/149‑1 (MQ=255)

                                                       aCGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcg                                                                                            >  2:1598583/1‑149 (MQ=255)

                                                        cGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                           <  4:345781/149‑1 (MQ=255)

                                                           cacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGtt                                                                                        >  1:910561/1‑149 (MQ=255)

                                                            acTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTc                                                                                       >  1:1758489/1‑149 (MQ=255)

                                                               gCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCgg                                                                                    <  1:138815/149‑1 (MQ=255)

                                                                cTTATTGCTTTTATTGTATTCTTATCTTTCCCGTGGTACCCGGAGCGAGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTTTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  4:924075/149‑1 (MQ=255)

                                                                cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  4:1488049/149‑1 (MQ=255)

                                                                cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  1:637438/149‑1 (MQ=255)

                                                                cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  1:1614682/149‑1 (MQ=255)

                                                                 ttATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAg                                                                                  >  1:157371/1‑149 (MQ=255)

                                                                        tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                           >  3:257044/1‑149 (MQ=255)

                                                                        tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                           <  4:77135/149‑1 (MQ=255)

                                                                        tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                           >  2:1743550/1‑149 (MQ=255)

                                                                        tctGATTTTATTCTTATCTTTCCCCTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                           >  3:178612/3‑149 (MQ=255)

                                                                           gATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                        <  2:1187978/149‑1 (MQ=255)

                                                                            aTTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGAc                                                                       <  1:1598583/149‑1 (MQ=255)

                                                                            aTTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGAc                                                                       <  2:1628008/149‑1 (MQ=255)

                                                                                 aTTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGAc                                                                  <  3:503435/149‑1 (MQ=255)

                                                                                     ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                              >  3:30536/1‑149 (MQ=255)

                                                                                     ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                              >  4:1415117/1‑149 (MQ=255)

                                                                                     ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                              <  1:1669522/149‑1 (MQ=255)

                                                                                      tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAtt                                                             >  3:21731/1‑149 (MQ=255)

                                                                                       aTCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTg                                                                                                  >  4:606113/1‑111 (MQ=255)

                                                                                       aTCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAttt                                                            >  2:811805/1‑149 (MQ=255)

                                                                                         cTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGc                                                          >  3:1686902/1‑149 (MQ=255)

                                                                                         cTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGc                                                          <  4:30536/149‑1 (MQ=255)

                                                                                             cccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATc                                                      <  1:811805/149‑1 (MQ=255)

                                                                                             cccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATc                                                      >  1:996408/1‑149 (MQ=255)

                                                                                               cATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                              <  3:1138111/139‑1 (MQ=255)

                                                                                                aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGc                                                   <  1:961193/149‑1 (MQ=255)

                                                                                                aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGc                                                   >  4:667673/1‑149 (MQ=255)

                                                                                                 tGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGTAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTGCAAATCCgtc                                                  <  3:1348522/149‑3 (MQ=255)

                                                                                                           aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGc                                        <  3:734518/149‑1 (MQ=255)

                                                                                                             cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                      <  4:1151092/149‑1 (MQ=255)

                                                                                                              gggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                                     >  3:1014862/1‑149 (MQ=255)

                                                                                                              gggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                                     >  1:379023/1‑149 (MQ=255)

                                                                                                              gggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                                     >  4:843239/1‑149 (MQ=255)

                                                                                                              gggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                                     >  1:203172/1‑149 (MQ=255)

                                                                                                               ggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGAtt                                                                                                                             <  3:681338/60‑1 (MQ=255)

                                                                                                               ggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGAtt                                                                                                                             >  4:681338/1‑60 (MQ=255)

                                                                                                                gACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  4:1210301/101‑1 (MQ=255)

                                                                                                                gACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGt                                   >  2:1315887/1‑149 (MQ=255)

                                                                                                                gACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGt                                   <  2:161450/149‑1 (MQ=255)

                                                                                                                         ccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGTGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGcc                          >  2:1377341/1‑149 (MQ=255)

                                                                                                                         ccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                   >  1:1121267/1‑108 (MQ=255)

                                                                                                                         ccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                   <  2:1121267/108‑1 (MQ=255)

                                                                                                                         ccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGcc                          <  2:1103287/149‑1 (MQ=255)

                                                                                                                           gCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  2:1240105/90‑1 (MQ=255)

                                                                                                                                 cgcgAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttctt                  <  1:141218/149‑1 (MQ=255)

                                                                                                                                  gcgAACGCCGAGGGATTTTAAATCGCTTGTGTGTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGTAGTCGAACCGGACTAGACGTATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGTCAAAttcttc                 <  4:944148/149‑1 (MQ=255)

                                                                                                                                   cgAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCa                <  2:157371/149‑1 (MQ=255)

                                                                                                                                    gAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAg               >  4:215143/1‑149 (MQ=255)

                                                                                                                                    gAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAg               <  4:1002152/149‑1 (MQ=255)

                                                                                                                                       cGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGcc            <  2:1068925/149‑1 (MQ=255)

                                                                                                                                         acaaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCtt          >  1:1514078/4‑149 (MQ=255)

                                                                                                                                           gAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc        <  4:1556529/149‑1 (MQ=255)

                                                                                                                                           gAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc        >  1:1179580/1‑149 (MQ=255)

                                                                                                                                           aaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc        >  1:1070648/2‑149 (MQ=255)

                                                                                                                                            aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  2:256363/73‑1 (MQ=255)

                                                                                                                                            aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                   <  4:567796/89‑1 (MQ=255)

                                                                                                                                            aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                   >  3:567796/1‑89 (MQ=255)

                                                                                                                                              ggATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGc     >  3:1143156/1‑149 (MQ=255)

                                                                                                                                               gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGcc    <  3:958294/149‑1 (MQ=255)

                                                                                                                                                aTTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                   <  3:774296/69‑1 (MQ=255)

AAGGAGGGTGTAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAG  >  NC_000913/1991718‑1992011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`