Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,367 G→A 7.8% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3670GA 7.8% 527.0 / 23.0 167intergenic (+28/‑488)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (77/77);  new base A (10/3);  total (87/80)
Fisher's exact test for biased strand distribution p-value = 8.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 174 total aligned reads displayed.

ACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTAT  >  NC_000913/2653220‑2653515
                                                                                                                                                   |                                                                                                                                                    
aCGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGa                                                                                                                                                     >  4:47847/1‑149 (MQ=255)
 cGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGaa                                                                                                                                                    >  4:1596946/1‑149 (MQ=255)
       ccGTGGTTGTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGc                                                                                                                                              >  4:150126/1‑149 (MQ=255)
           ggTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                                          <  3:294148/149‑1 (MQ=255)
            gTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCAGAATTTGGCGGttt                                                                                                                                         >  4:1467104/1‑149 (MQ=255)
              tttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGttttt                                                                                                                                       <  2:43431/149‑1 (MQ=255)
                 gTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAt                                                                                                                                    >  2:251663/1‑149 (MQ=255)
                  ttAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAtt                                                                                                                                   >  3:1464782/1‑149 (MQ=255)
                   tAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTg                                                                                                                                  <  2:427974/149‑1 (MQ=255)
                    aGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttttt                                                                                                                                      >  4:834936/1‑144 (MQ=255)
                    aGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGc                                                                                                                                 <  3:1363800/149‑1 (MQ=255)
                      cACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGTTa                                                                                                                               >  2:1009522/1‑149 (MQ=255)
                          cgcgACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATACGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCt                                                                                                                           <  2:1509127/149‑1 (MQ=255)
                              aCGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGtt                                                                                                                       <  3:732582/149‑1 (MQ=255)
                                gCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcg                                                                                                                     >  3:1478141/1‑149 (MQ=255)
                                  tGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACTGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgcg                                                                                                                   <  4:665632/149‑1 (MQ=255)
                                    gATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGc                                                                                                                 >  3:1680277/1‑149 (MQ=255)
                                         gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                            <  3:1023776/149‑1 (MQ=255)
                                         gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                            >  3:62587/1‑149 (MQ=255)
                                          ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTcc                                                                                                           >  3:608497/1‑149 (MQ=255)
                                           cAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAAAAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCca                                                                                                          <  3:49405/149‑1 (MQ=255)
                                            aaaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                                                                         >  2:152255/1‑149 (MQ=255)
                                             aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagc                                                                                                        >  4:440744/1‑149 (MQ=255)
                                                gTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAgg                                                                                                     >  4:502101/1‑149 (MQ=255)
                                                gTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAgg                                                                                                     >  4:962247/1‑149 (MQ=255)
                                                 tGAAACTGTACGACGGCGAATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCCATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTCCGCGGCCTTCCCAGCAGGt                                                                                                    >  4:595797/1‑149 (MQ=255)
                                                  gAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAGATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAg                                                                                                                              >  1:157062/1‑122 (MQ=255)
                                                  gAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAg                                                                                                                              <  2:157062/122‑1 (MQ=255)
                                                  gAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                   <  3:768727/149‑1 (MQ=255)
                                                  gAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                   <  4:1478141/149‑1 (MQ=255)
                                                         tACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgt                                                                                            <  1:372583/149‑1 (MQ=255)
                                                          acgacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                                                                           <  3:962247/149‑1 (MQ=255)
                                                            gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                                                                         <  2:769343/149‑1 (MQ=255)
                                                            gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                                                                         >  2:995471/1‑149 (MQ=255)
                                                            gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                                                                         >  1:832733/1‑149 (MQ=255)
                                                                  gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                                                 <  2:378890/87‑1 (MQ=255)
                                                                  gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                   >  4:1407388/1‑149 (MQ=255)
                                                                  gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                   <  3:440744/149‑1 (MQ=255)
                                                                  gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                   <  3:215343/149‑1 (MQ=255)
                                                                       gAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAAc                                                                              >  3:1081794/1‑149 (MQ=255)
                                                                         gTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGc                                                                            >  2:121765/1‑149 (MQ=255)
                                                                         gTGAATGGGGCGCGCGGGCAGAGTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                   <  3:1128330/142‑1 (MQ=255)
                                                                                gggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCt                                                                     >  4:724968/1‑149 (MQ=255)
                                                                                    gcgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac                                                                 <  3:314412/149‑1 (MQ=255)
                                                                                    gcgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGttt                                                                                                                                         >  4:590629/1‑77 (MQ=37)
                                                                                    gcgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGttt                                                                                                                                         <  3:590629/77‑1 (MQ=37)
                                                                                     cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                                                                <  3:338999/149‑1 (MQ=255)
                                                                                          gCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgt                                                                                            >  1:456379/1‑116 (MQ=255)
                                                                                             gATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACa                                                        >  4:687007/1‑149 (MQ=255)
                                                                                               tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAAt                                                      >  3:1085811/1‑149 (MQ=255)
                                                                                                ttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCACCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATa                                                     <  3:150126/149‑1 (MQ=255)
                                                                                                  aCCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                                                                      >  1:760939/1‑114 (MQ=255)
                                                                                                    cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCa                                                 >  2:623062/1‑149 (MQ=255)
                                                                                                     ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                <  2:723836/149‑1 (MQ=255)
                                                                                                     ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                <  3:502101/149‑1 (MQ=255)
                                                                                                      gTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAcc                                               >  3:1049263/1‑149 (MQ=255)
                                                                                                          aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCa                                              <  4:789468/146‑1 (MQ=255)
                                                                                                          aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCa                                              >  3:789468/1‑146 (MQ=255)
                                                                                                          aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTa                                           >  1:1397953/1‑149 (MQ=255)
                                                                                                           gCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTaa                                          <  1:152255/149‑1 (MQ=255)
                                                                                                               gTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActct                                      <  4:365868/149‑1 (MQ=255)
                                                                                                               gTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActct                                      >  2:149448/1‑149 (MQ=255)
                                                                                                                  aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtt                                   >  1:1003089/1‑149 (MQ=255)
                                                                                                                  aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtt                                   >  1:1524689/1‑149 (MQ=255)
                                                                                                                   aaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGCTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                                                                >  2:690247/1‑119 (MQ=255)
                                                                                                                    aTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                                                                >  3:632949/1‑118 (MQ=255)
                                                                                                                     tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCTAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                >  3:271776/1‑149 (MQ=255)
                                                                                                                     tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                <  2:968419/149‑1 (MQ=255)
                                                                                                                       aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGt                              <  1:1164679/149‑1 (MQ=255)
                                                                                                                         tATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGAc                                                                                                <  3:120252/81‑1 (MQ=255)
                                                                                                                         tATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCa                            >  1:231901/1‑149 (MQ=255)
                                                                                                                          aTTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCaa                           <  1:870674/149‑1 (MQ=255)
                                                                                                                           ttttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGTAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTCACTCTCTTTTTGTCAAg                          >  3:184599/1‑149 (MQ=255)
                                                                                                                                 aGGCAATACAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAag                    >  3:1590289/1‑149 (MQ=255)
                                                                                                                                 aGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                >  4:400277/1‑121 (MQ=255)
                                                                                                                                 aGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAag                    >  3:1248144/1‑149 (MQ=255)
                                                                                                                                  gtcAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   >  1:405309/3‑149 (MQ=255)
                                                                                                                                  ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   >  4:241400/1‑149 (MQ=255)
                                                                                                                                  ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   <  4:322173/149‑1 (MQ=255)
                                                                                                                                    cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagaga                 <  2:832733/149‑1 (MQ=255)
                                                                                                                                    cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   >  4:836261/1‑147 (MQ=255)
                                                                                                                                    cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   <  3:836261/147‑1 (MQ=255)
                                                                                                                                      aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTCCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   <  2:510676/145‑1 (MQ=255)
                                                                                                                                      aTAAAAAACCGCCGAATTGGGCGGTTTTTTATTGCTAGTCGGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                   >  1:510676/1‑145 (MQ=255)
                                                                                                                                       tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaaa                                                          >  3:799593/1‑105 (MQ=255)
                                                                                                                                       tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTa              <  1:1587779/149‑1 (MQ=255)
                                                                                                                                          aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTTTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActctc                                     <  3:231814/123‑1 (MQ=255)
                                                                                                                                          aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActctc                                     >  4:231814/1‑123 (MQ=255)
                                                                                                                                          aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatt           >  4:1036140/1‑149 (MQ=255)
                                                                                                                                          aaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatt           >  4:1604587/1‑149 (MQ=255)
                                                                                                                                           aaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatta          >  4:884879/1‑149 (MQ=255)
                                                                                                                                           aaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGCTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGATAGTAatta          >  1:60871/1‑149 (MQ=255)
                                                                                                                                           aaaCCGCCAAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatta          >  4:1660213/1‑149 (MQ=255)
                                                                                                                                            aaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAattat         >  2:1703148/1‑149 (MQ=255)
                                                                                                                                            aaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAattat         >  1:250132/1‑149 (MQ=255)
                                                                                                                                            aaCCGCAAAATTCGGCGGTGTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGCAattat         >  4:1093055/1‑149 (MQ=38)
                                                                                                                                              ccgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTg       <  4:808525/149‑1 (MQ=255)
                                                                                                                                               cgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                                                                  >  4:1173186/1‑89 (MQ=255)
                                                                                                                                               cgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGt      <  4:62587/149‑1 (MQ=255)
                                                                                                                                                   gAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtttat  >  4:1697403/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTAT  >  NC_000913/2653220‑2653515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: