Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,306 A→T 5.5% intergenic (‑243/+29) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3060AT 5.5% 627.7 / 19.9 181intergenic (‑243/+29)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base A (90/81);  new base T (4/6);  total (94/87)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 182 total aligned reads displayed.

CGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGC  >  NC_000913/2704159‑2704453
                                                                                                                                                   |                                                                                                                                                   
cGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATaa                                                                                                                                                    >  3:1234430/1‑149 (MQ=255)
   tataTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACgg                                                                                                                                                 >  4:1504810/1‑149 (MQ=255)
    atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAAATTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                <  1:482249/149‑1 (MQ=255)
    atatGGAGACCAACGCTGTTTCGTGTGCCGTGGCTACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACTACGACTGGGGATGTCGTTAGCGATGGGAGATTTATCTCCTAAATAATTCACGTTTTCGCCATAAAGGc                                                                                                                                                <  2:1185278/149‑1 (MQ=255)
     tatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCg                                                                                                                                               >  1:1449271/1‑149 (MQ=255)
       tGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGAc                                                                                                                                             >  1:688861/1‑149 (MQ=255)
       tGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAAc                                                                                                                                                   >  4:1600760/1‑143 (MQ=255)
       tGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGAc                                                                                                                                             <  2:1289026/149‑1 (MQ=255)
       tGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGAc                                                                                                                                             >  4:407017/1‑149 (MQ=255)
        ggggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACa                                                                                                                                            >  3:9378/1‑149 (MQ=255)
        ggggACCAAAACTGTTTTGTGTGCCGTGGGAACCTCGGGGTGGCAAACCTAAACTTCGGGGGGATATTTTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTTTCTCATAAAAAATTCACGTTGTCGCCATAAAGGCGACa                                                                                                                                            >  2:475104/1‑149 (MQ=255)
         gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATCCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACaa                                                                                                                                           <  2:206011/149‑1 (MQ=255)
          ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAc                                                                                                                                          >  1:469745/1‑149 (MQ=255)
          ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAc                                                                                                                                          <  3:407017/149‑1 (MQ=255)
            aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGt                                                                                                                                        <  3:1674355/149‑1 (MQ=255)
            aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGt                                                                                                                                        <  2:727528/149‑1 (MQ=255)
              cAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACGTGa                                                                                                                                      <  1:1212022/149‑1 (MQ=255)
              cAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGa                                                                                                                                      <  3:1102383/149‑1 (MQ=255)
               aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGGATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGaa                                                                                                                                     >  1:196272/1‑149 (MQ=255)
               aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACGTGaa                                                                                                                                     <  3:364344/149‑1 (MQ=255)
               aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGaa                                                                                                                                     >  4:210892/1‑149 (MQ=255)
               aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGaa                                                                                                                                     >  2:1468585/1‑149 (MQ=255)
                aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                    <  4:1719647/149‑1 (MQ=255)
                 cGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACg                                                                                                                                   >  3:924134/1‑149 (MQ=255)
                   cTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGaa                                                                                                                                 <  1:163300/149‑1 (MQ=255)
                      tttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGAt                                                                                                                              <  1:575562/149‑1 (MQ=255)
                        tCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATgg                                                                                                                            <  3:1709650/149‑1 (MQ=255)
                           tgtgCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTa                                                                                                                         >  3:884323/1‑149 (MQ=255)
                                cGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAAt                                                                                                                    <  1:957363/149‑1 (MQ=255)
                                 gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                   <  1:49175/149‑1 (MQ=255)
                                 gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                   >  1:1334595/1‑149 (MQ=255)
                                  tGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATgg                                                                                                                  >  3:755093/1‑149 (MQ=255)
                                  tGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATgg                                                                                                                  >  4:743154/1‑149 (MQ=255)
                                   ggCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGa                                                                                                                 >  1:795007/1‑149 (MQ=255)
                                    gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                >  1:1194598/1‑149 (MQ=255)
                                    gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                >  1:1055971/1‑149 (MQ=255)
                                       aCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                          >  2:360099/1‑136 (MQ=255)
                                       aCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                          <  1:360099/136‑1 (MQ=255)
                                       aCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgcc                                                                                                             >  1:46386/1‑149 (MQ=255)
                                         cTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgccgc                                                                                                           >  2:1006930/1‑149 (MQ=255)
                                            gAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                          >  1:1006405/1‑115 (MQ=255)
                                               gTGCCAACCTTAAACTTCGGGGGCATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                     >  4:109593/1‑149 (MQ=255)
                                                 gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                   <  3:1462948/149‑1 (MQ=255)
                                                 gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                   >  3:1086553/1‑149 (MQ=255)
                                                 gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                   <  4:1068874/149‑1 (MQ=255)
                                                  ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAc                                                                                                                                          >  2:898358/1‑109 (MQ=255)
                                                  ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAc                                                                                                                                          <  1:898358/109‑1 (MQ=255)
                                                  ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGAc                                                                                                  >  4:323457/1‑149 (MQ=255)
                                                   cAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACt                                                                                                 >  1:604311/1‑149 (MQ=255)
                                                   cAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACt                                                                                                 >  1:1018968/1‑149 (MQ=255)
                                                      ccTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTaa                                                                                              <  4:1371291/149‑1 (MQ=255)
                                                      ccTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTaa                                                                                              >  1:55063/1‑149 (MQ=255)
                                                        ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                             >  1:485275/1‑116 (MQ=255)
                                                        ttAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                            >  3:636606/1‑149 (MQ=255)
                                                          aaaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTAt                                                                                                                        >  2:1001204/1‑119 (MQ=255)
                                                          aaaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTAt                                                                                                                        <  1:1001204/119‑1 (MQ=255)
                                                            aCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAg                                                                                        >  3:140937/1‑149 (MQ=255)
                                                              ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAc                                                                                      >  2:296542/1‑149 (MQ=255)
                                                              ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAc                                                                                      <  1:296542/149‑1 (MQ=255)
                                                                  ggggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAga                                                                                  >  3:152292/1‑148 (MQ=255)
                                                                   gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTc                                                                                 <  4:25529/149‑1 (MQ=255)
                                                                   gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTc                                                                                 >  3:1580135/1‑149 (MQ=255)
                                                                        tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCa                                                                            >  1:1185092/1‑149 (MQ=255)
                                                                          ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                            >  2:1107081/1‑131 (MQ=255)
                                                                             tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                       >  3:1382372/1‑149 (MQ=255)
                                                                             tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                       >  3:1178622/1‑149 (MQ=255)
                                                                                acacAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTg                                                                                                                                       <  2:489119/82‑1 (MQ=255)
                                                                                acacAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCctt                                                                    <  4:1322657/149‑1 (MQ=255)
                                                                                  acaACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGa                                                                                                                 <  3:507600/102‑1 (MQ=255)
                                                                                  acaACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCcttct                                                                  >  1:881933/1‑149 (MQ=255)
                                                                                  acaACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCct                                                                     <  4:1151772/146‑1 (MQ=255)
                                                                                    aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                >  4:128922/1‑149 (MQ=255)
                                                                                         cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTAt                                                           <  3:27402/149‑1 (MQ=255)
                                                                                          gggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                          <  2:1596895/149‑1 (MQ=255)
                                                                                           ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCg                                                         >  2:594173/1‑149 (MQ=255)
                                                                                              gATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAg                                                      <  2:124504/149‑1 (MQ=255)
                                                                                               aTGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGc                                                     <  1:709554/149‑1 (MQ=255)
                                                                                                tGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGTCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCt                                                    >  2:1708394/1‑149 (MQ=255)
                                                                                                  tCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCATATCGAAGCTCa                                                  <  3:128922/149‑1 (MQ=255)
                                                                                                    gTTAGCCACGGGAGATTTATCACATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTTCTTATCGAAGCTCATc                                                >  2:702673/1‑149 (MQ=255)
                                                                                                     ttAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATcc                                               <  3:210892/149‑1 (MQ=255)
                                                                                                        gCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                            >  1:1714597/1‑149 (MQ=255)
                                                                                                                    ttATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGccc                                <  2:1449271/149‑1 (MQ=255)
                                                                                                                         tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                           >  4:887257/1‑149 (MQ=255)
                                                                                                                            taaataaTTCACGTTGTCGCCATAACTGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCtt                                                             >  4:630188/1‑112 (MQ=255)
                                                                                                                             aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                       <  2:1232349/149‑1 (MQ=255)
                                                                                                                              aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAg                      >  3:535770/1‑149 (MQ=255)
                                                                                                                              aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAg                      >  1:1498978/1‑149 (MQ=255)
                                                                                                                                taatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGAt                    <  4:9378/149‑1 (MQ=255)
                                                                                                                                 aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGACGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCAGCCAGATAGCCGTTGCCCGACCGACCAGAtt                   <  3:6188/149‑1 (MQ=255)
                                                                                                                                      aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGt                                                                                  <  4:139697/81‑1 (MQ=255)
                                                                                                                                        gTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTcc            >  2:682860/1‑149 (MQ=255)
                                                                                                                                         ttGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg           >  4:102475/1‑149 (MQ=255)
                                                                                                                                           gTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGc         >  1:382844/1‑149 (MQ=255)
                                                                                                                                             cGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGc                <  2:1665219/140‑1 (MQ=255)
                                                                                                                                             cGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcac       >  3:104550/1‑149 (MQ=255)
                                                                                                                                               ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaa     >  3:868744/1‑149 (MQ=255)
                                                                                                                                                cATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa    >  2:994466/1‑149 (MQ=255)
                                                                                                                                                cATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa    >  3:1424822/1‑149 (MQ=255)
                                                                                                                                                  tAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGc  >  2:1358186/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGC  >  NC_000913/2704159‑2704453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: